세포질내 정자주입법(ICSI)에 있어서 정자흡입 및 난자내 주입방법에 관한 연구

이택후,김항진,송건호,김대근,전상식,박윤규,서태광,전병균,류은경,이은숙,문진수,김광철 경북대학교 의학연구소 2000 경북대학교병원의학연구소논문집 Vol.4 No.1

Study on Method of Sperm Aspiration and Injection into an Oocyte in Intracytoplasmic Sperm Injection(ICSI) Immobilization of spermatozoa prior to intracytoplasmic sperm iniection(ICSI) sometimes results in crooked tail and this makes it difficult to aspirate sperm into an injection pipette tail first. Head-first sperm aspiration into an injection pipette avoid this problem due to the bigger size of the sperm head. The effect of head or tail-first sperm injection into an oocyte on fertilization cleavage, percentage of grade I embryos and development to blastocyst stage in ICSI program has been studied. A single living immobilized spermatozoa from oligoasthenozoospermic patient was injected into an oocyte head-first or tail-first according to the treatment. Eighteen hours after microinjection, oocytes ware inspected for survival and fertilization Fertilized oocytes with two pronuclei were cultured in 30μl drop of mHTF supplemented with 10% heat-inactivated follicular fluid(FF) at 37℃. On day 2. embryo transfer was performed with cleaved embryos. The remaining 2-8 cell stage embryos were co-cultured with BRL cells in mHTF + 10% FF for 72 hours and the developmental stage was observed. The data were analyzed by Analysis of Variance. A total of 164 oocytes from 36 cycles were assigned to earth treatment and ICSI was performed(88 head-first, tail-first). The rates of normal fertilization were 81.8% and 76.3% for head-first and tail-first, respectively. Of the fertilized oocytes, the percentage of cleaved embryos and the percentage of grade 1 embryo among cleaved embryos were 88.9% and 68.8%, 93.1% and 74.1% for head-first and tail-first, respectively. Of the 2-8 cell embryos cultured, 44.4%(16/36) and 50.0%(10/20) for head first and tail first, respectively developed to blastocyst stage. There were no differences in fertilization, cleavage, rates of grade 1 embryos, and development to blastocyst stage. In conclusion, head-first or tail-first sperm injection into an oocyte in ICSI program does not affect fertilization and subsequent embryo development to blastocyst stage in vitro.

확대된 여가제약 척도의 최적범주

이창진(Lee, Chang-Jin),김응준(Kim, Eung-Joon) 한국웰니스학회 2017 한국웰니스학회지 Vol.12 No.1

본 연구는 김응준(2012)이 Rasch 모형을 적용하여 선행연구에서 개발된 확대된 여가 제약 척도의 심리측정학적 특성 결과를 바탕으로 최적범주를 탐색하는데 목적이 있었다. 이 목적을 달성하기 위해 대학교 410명의 학생들을 대상으로 조사를 실시하고 통계분석하였다. 사용된 척도는 Crawford와 Godbey(1987)에 의해 제시된 여가제약 척도를 지현진과 이철원(2003)이 수정 보완하고, 김응준(2006)에 의하여 타당화 시킨 확대된 여가 제약 척도를 조사도구로 사용하였다. 수거된 자료 중 410명의 자료를 SPSS 23.0프로그램을 이용하여 빈도분석, 기술통계분석을 실시하였다. 최적범주를 탐색하고자 WINSTEPS 3.92 (Linacre, 2016)을 사용하여, 척도수의 적절성, 문항적합도와 문항난이도 등을 산출하였다. 연구목적에 따른 연구결론으로는 첫째, 문항의 반응범주는 문제가 있는 것으로 확인되어 기존 5점 범주 대신 4점 범주로 수정되는 것이 타당하였다. 둘째, 문항적합도를 분석한 결과 22문항은 적합하였다. 셋째, 척도의 응답자 신뢰도가 낮게 확인되어 문항난이도를 고려한 문항순서의 변경이 필요하였다. 넷째, 개인 능력점수와 난이도를 교차하여 비교한 결과, 개인 능력점수 분포와 문항난이도 분포는 적절하였다. The purpose of this study was to investigate the extended leisure constraint questionnaire’s optimal categorization in undergraduate students. To accomplish study purpose, complete enumeration survey and statistical analysis were conducted for 410 students enrolled university. A research tool used in this study was an extended leisure constraint scale developed by Eung-Joon KIM (2012), which was originally from Crawford & Godbey(1987). Total 22 questions were used with 5 point Likert scale and high point stands for higher the leisure constraint. Frequency analysis was conducted with SPSS 23. An assumption to test Rasch Model, which is satisfaction of unidimensionality, is regarded through theoretical aspects, and WINSTEPS 3.92(Linacre, 2016) program is used to test the goodness-of-fit of items. Statistical analysis showed results as follows. First, 4-point rating scale was appropriate for the extended leisure constraint scale. Second, 22 items of the extended leisure constraint scale were suitable in item fitness and item difficulty to undergraduate students. Third, the extended leisure constraint scale was somewhat suitable in person reliability of item separation. Fourth, the person ability score of the extended leisure constraint scale was distributed appropriately.

인대내 거대자궁 근종 증례

김윤진,전명권,이응수,최형민 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.4

Uterine myomas are the most common pelvic tumor in women. These benign tumor generally arise in the uterinie wall and intraligamentary myomas are relatively rare cases. We experienced intraligamentary huge myoma. Preoperative GnRH(Gonadotropin Releasing Hormone) agonist therapy was done but the effect was nearly absent. Hystrectomy was done without complication. We report this case with brief review of literatures.

직장암 수술 후 국소재발과 예후

김태윤,백무준,김성용,신응진,박내경,이문수,김창호,송옥평 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1

Locally recurrent rectal cancer is a difficult clinical problem. Local recurrence following treatment for rectal cancer is a major cause of morbidity and mortality. The reported range of local recurrence rates following surgical treatment for rectal cancer has varied from 4% to 50%. We retrospectively reviewed the charts of 142 rectal cancer patients from January 1991 to December 1999. Analysis of patients factors included age, gender, type of surgery, and adjuvant therapy. Tumors were assessed for level, stage, and grade. Local recurrence and distant metastases were also documented. Variables influencing local recurrence in this group were identified and disease-free survival is determined. Of the 142 patients(74 males, 68 females; mean age 62.6 years), 137 consecutive patients operated on for rectal cancer and followed up to December 2000 (range 23-118months) were analyzed. Local recurrence rate was 18.3%. Age and sex of patients, type of surgery, location of tumor in the rectum, size, morphology and grading of the tumor were all unrelated to the event under investigation. At Cox regression, the Dukes stage and the postoperative radiotherapy were the only independent prognostic factors for local recurrence, and Dukes stage (Hazard ratio=2.89, p<0.001) and local recurrence (Hazard ratio=3.31, p<0.01) were the only independent factors associated with improved survival.

대동맥판막에 발생한 유두상 섬유탄력종 1례

김응중,최재성,이명묵,김어진 동국대학교 의학연구소 2009 東國醫學 Vol.16 No.1

유두상 섬유탄력종은 주로 심장판막에 발생하는 원발성 종양으로 심장종양 중 두 번째로 많은 양성종양이나 전체적으로 그 빈도는 매우 드물다. 78세 여자 환자가 흉부 불편감을 주소로 내원해서 대동맥판막에 위치한 유두상 섬유탄력종이 발견되어 개심술 하에 대동맥판막의 손상 없이 종양을 제거하였기에 문헌고찰과 더불어 이를 보고하는 바이다. Cardiac papillary fibroelastoma is the rare primary cardiac tumor, though it is the second most common benign cardiac tumor. It usually involves the cardiac valve. Papillary fibroelastoma of the aortic valve was found in a 78-year-old woman with chest discomfort and it was excised without valvular injury under cardiopulmonary bypass. Thus we report the present case with relevant review of the literatures.

오존層保護를 위한 分爭解決節次

김효진,엄응용 경운대학교 산업기술연구소 1999 産業技術硏究論文誌 Vol.1 No.1(B)

This Article explores state responsibility to the international community as a whole and injured states in particular for the damage occurring from the production and use of ozone depleting substances. This Article further explores the remedies available to the international community and to individual states injured by ozone layer depletion. The Article describes international dispute resolution techniques that states may employ in the face of continued polluting activities by other states.

중복 자궁 경부, 맹낭성 종질중격 및 동측 신장발생부전을 가진 교통성 중복자궁 1례

김윤진,전명권,이응수,최형민 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.4

The class of uterine malformation known as communicating uteri is characterized by two uterocervical cavities connected by an isthmic communication. We experienced case of communicating double uterus with double cervix, blind hemivagina and ipsilateral renal agenesis. The complaint of the patient was odorous vaginal discharge with lower abdominal pain unrelated with menstrual cycle. We confirmed these Mullerian anomalies by transvaginal ultrasound. IVP(intravenous pyelography), MRI(magnetic resonance imaging), hysterosalpingography and hysteroscopy. After vaginal septoplasty, her complaint was subsided. We report this case with brief review of literatures.

"人類의 共同遺産" 槪念의 法的 意味

김효진,엄응용 경운대학교 산업기술연구소 2001 産業技術硏究論文誌 Vol.3 No.2(B)

After the world war Ⅱ, an international society has been concerned about the outside region of national jurisdiction(Deep seabed, Outer Space, Moon, South Pole etc), and the development and conservation of its resources. As a result, Antarctic Treaty(1959), Outer Space Treaty(1967), the Moon Treaty(1979), and UNCLOS(1982) were concluded. Above all, through the treaties common Heritage of Mankind(CHM) was induced expressly or analogically as a new approach for the development and conservation of International Common Spaces. However, the question at issue is that the concept, character , extent, and regulations of CHM which was induced by the treaties have been interpreted and applied according to each treaty or standpoint of each nation. This lead to denunciation on the status of the CHM in international law. By the way, the core cause of this denunciation could be that there's no right legal definition on the terms of the CHM. It means that a prior question on the status of the CHM from the point of the international law is to analyze and examine closely the terms of this concept and define the legal implication. Therefore, I am willing to describe the appearance and development of the concept of CHM, analyze the legal significance on each term of the concept and evaluate generally the legal position of it through this study.

비장애 아동과 전반적 발달장애 아동에서 회전 후 안구진탕 기간의 비교

김진미,송지원,홍은경,김성희,김경미 대한감각통합치료학회 2005 대한감각통합치료학회지 Vol.3 No.1

Objective : The purpose of this study was to get the mean of duration of post-rotatory nystagmus test in normal children and to differentiate the duration of post-rotatory nystagmus test between normal children and children with pervasive developmental disorder. Method : 84 subjects were between 3 and 5 years of age and consisted of 64 normal children and 20 children with the pervasive developmental disorder. Analysis of the data was done by using t-test and ANOVA. Results : The results were as follows: 1. Range of duration of post-rotatory nyatagmus test in normal children was 5-22second on left and 7-21 second on right and the mean was 12.63 second on left and 12.59 second on right. 2. Range of duration of post-rotatory nystagmus test in children with the pervasive developmental disorder was 3-11 second on both and the mean was 5.65 second on left and 5.60 second on right. 3. There was no significant difference between males and females with normal children in duration of post-rotatory nystagmus test. However, there was a significant difference of the mean duration between 3 and 5 years old normal group. 4. Children with pervasive developmental disorder significantly have relatively lower duration than the duration of post-rotatory nystagmus test of normal children. Conclusions : The results of the study showed significant difference between normal children and children with pervasive developmental disorder in duration of post-rotatory nystagmus test and suggest that they could be applied to the baseline of clinical therapy.

참치 뼈를 이용한 Hydroxyapatite 세라믹 복합체의 합성 및 생체 친화성(제3보) : 인공체액에서의 Hydroxyapatite 세라믹 복합체간의 결합의 전자현미경 관찰 SEM Photographs of Bonding Properties between Hydroxyapatite Ceramics Composites in the Simulated Body Fluid

김세권,최진삼,이창국,변희국,전유진,이응호,박인용 대전산업대학교 반도체기술연구소 1999 半導體技術硏究所報 Vol.1 No.-

참치 뼈에서 추출한 hydroxyapatite를 출발물질로 여러 가지 세라믹 복합체를 제조하여 인공체액에서의 hydroxyapatite 복합체간의 화학결합을 조사하였다. hydroxyapatite 복합체들은 인공체액에서 4주 후부터 화학 결합성질을 나타내었다. 결합강도는 bioglass가 가장 강하게 나타났으며, 조성에 따른 의존성은 관찰할 수 없었다. 인공체액에서 복합체들은 이들의 경계면에서 불균일 핵생성 및 성장에 의해 화학적 결합으로 이루어졌다. Chemical bonding was investigated in the simulated body fluid of several selected hydroxyapatite-containing composites. The hydroxyapatite-containing composites chemically bonded with each other in the simulated body fluid after 4 weeks. Bioglass was strongly bonded in the simulated body fluid, but bonding strength was not depended on composition. Their composite bodies were chemically bonded by heterogeneous nucleation and growth at the interfaces of the specimens in the simulated body fluid.