HL-60 세포의 성장에 대한 제주자생 해조류의 효과

김상철,박수영,김병석,강지훈,김세재 제주대학교 생명과학기술혁신센터 2002 제주생명과학연구 Vol.5 No.-

The purpose of the present study is to examine the inhibitory effect of extracts of marine algae growing in Jeju on the growth of HL-60 leukemia cells. The effect was observed by measurement of metabolic activity and counting of viable cells. The methanol extracts on 36 species of marine algae growing in Jeju were screened for cytotoxic effect at the concentration of 100㎍/㎖. In results, the inhibitory effect on the growth was observed in the treatment of crude extracts on 7 species of marine algae. In particular, Dictyota dichotoma, Myelophycus simplex, Laurencia okamurae, Plocamium telfairiae, Petalonia binghamiae, Codium minus marketdly inhibited the growth of HL-60 cells.

제주자생 해조류의 세포성장 억제효과

김상철,박수영,김병석,강희경 제주대학교 생명과학연구소 2002 제주생명과학연구 Vol.5 No.6

The purpose of the present study is to examine the inhibitory effect of extracts of marine algae growing in Jeju on the growth of leukemia cells, adenocarcinoma cells, normal cell. The effect was observed by measurement of metabolic activity using colorimetric MTT assay. The methanol extracts on 36 species of marine algae growing in Jeju were screened for cytotoxic activity against HL-60 cells was found in 5 specices of Marine algae. In particular, Scytosiphon lomentaria, Lithophyllum okamurae. Hydroclathrus clathratus, Dictyopteris undulata, also showing similar cytotoxity to KG-1 cells. But, they did not show similar cytotoxicity to SNU-16 cells, HCT-15 cells. Lithophyllum okamurae, Dictyopteris undulata markedly inhibited the growth of CCD-25Lu normal cell.

3차 의료기관 응급실로 내원한 안면부 열상 환자에 대한 임상적 고찰

김양원,안성훈,류석용,김홍용,전병민,김기태 대한응급의학회 2001 대한응급의학회지 Vol.12 No.2

Background: Facial laceration is a common clinical problem in the emergency room that is frequently associated with other craniofacial injuries. It elicits an emotional response from the patient because of the underlying concern of permanent scar and secondary facial disfigurement, so they demand prompt and adequate treatment. The aim of this study is to analyze the incidence, etiology, depth, and site of facial lacerations to provide basic data for further understanding. Method: This study reviewed 1,043 facial-laceration patients treated in the emergency room at Pusan Paik Hospital between March 1999 and February 2000. This retrospective study was done by reviewing and analyzing the sex and age distributions, the monthly and daily distributions, the causes of injury, the types of injury, the sites of injury, the lengths of the lacerations, and associated injuries. Results: The sex ratio of men to wemen was 2.4:1, and the 0~10 age group was at the top of the age distribution. The number of patients was the highest on sundays and during March. About 9.2% of the patients visited the emergency room between 10:00 P.M. and 11:00 P.M. Falls(38.1%) were the most common cause of facial laceration, and deep laceration(51.8%) was the most common type of facial laceration. The most common laceration site was the forehead, followed by the chin and the nose. The most common associated craniofacial injury was facial bone fracture(39%), Facial-lacerations were sutured by plastic surgeons(80%), emergency physicians(10%), and oromaxillofacial surgeons(10%). We found statistical significance in the types and lengths of lacerations between men and women, among age groups(p<0.05). Conclusion: Facial laceration is one of the most common craniofacial injuries in the emergency room. By now, most lacerations have been repaired by plastic surgeons, But, the causes of lacerations are diverse and many patients are admitted due to the associated injuries. Therefore, emergency physicians should participate more aggressively in the care of facial-laceration patients to improve the outcome of the patients.

하악골 골절의 임상적 연구

양병은,김용관,유준영,장현석 大韓顎顔面成形再建外科學會 1997 Maxillofacial Plastic Reconstructive Surgery Vol.19 No.2

The mandible plays a crucial role in the vital human functions of breathing, eating, and speaking. No other bone in the body has such a distinctive shape and function. Because of its prominence in the facial structure, the mandible is highly prone to fracture. A chinical study on 122 patients with mandibular fracture who visited Kangnam General Hospital during 4 year(1992-1995) was done by analysing sex, age, cause, fracture site, teeth involvement, treatment method, complication and involvement of other body part. The results obtained were as follows : 1. The occurrence was more frequent in male than in female with the ratio of 5.4 : 1 and most frequently in twenties. 2. Violence was the most common cause of facial mandibular fracture. 3. In mandibular fracture, fracture site was average 1.5 sites, most frequently in symphysis and simple fracture was 86.1%. 4. As treatment methods, open reduction(78.6%) was used more frequently than closed reduction(21.4%) 5. Post-operational complication occurred in 27.0% of the cases. 6. Other injuries that were related to maxillofacial fracture occurred in 25.4%.

제7차 교육과정 중 ICT 활용이 수업에 미치는 영향 및 개선

조병제,김하석 대불대학교 2003 論文集 Vol.9 No.1

With the introduction of ICT of the 7th curriculum this study is to analyze the process of spreading ICT in Korea and the appearance of execution now from more concete and positive point of view through the case study by the teachers who tried to change and improve the whole schools. And this study is to attain the ultimate goal that the education if ICT utility pursues and to suggest the direction to the substantial progress. From the result of this research above, it may be summed up as follows. 1. As for ICT utility instruction, the guide for ICT utility by the subject on the national level was reinfoeced. So, it needs the force of curriculum, and it should be applied to the first-line schools. 2. The utilization could be increased by only making the teachers easy to reedit adequate to the school and personal level at first hand after developing the materials for the instruction of ICT utility by the subject and constructing the utility system. 3. The policy of amount-centered use of information technology in education brought about the waste of a lot of budgets. Also, it brought about the result of mass production of the defective personal computers, we can not use them now, because of absence of personnel who could control the instruments and of being restricted by time for the teachers. 4. In addition, the mind of use of information technology in education still lacks for the whole teachers and students in that the disharmony with the traditional infrastructure and information infrastructure is importantly connected with the improvement of the quality of instriction and the learning effect for the students. Hereafter, the capacity of ICT utlility for the teachers is short of only training the information technology in teachers. And it should be applied to the teachers' promotion and personal affairs complexly. The first-line schools are available in utility effectively only when the teachers who lead the information technology in education should be continuously trained. It presented that overcoming the utility education of functional ICT maximizes the effect only when it a uniform instruction should be sublated and ICT is utilized in all the subjects. In particular, the effect on a dance and instruction in the arts high schools is great. This study is to present clearly that we can get sufficient effect only when it grafts that to the field of schools together through the steady development and improvement of the teaching-learning method as well as the curriculum on a basis of analysis of ICT utility in several literatures and the 7th curriculum till now.

12주간 축구훈련이 정신지체아의 심박수에 미치는 영향

이창규,김병완,현광석,조병준 대전대학교 기초과학연구소 1997 自然科學 Vol.8 No.1

12주간의 축구훈련이 정신지체아의 심박수에 미치는 영향을 알아보기 위하여 훈련되지않은 14명의 정신지체 남자 중학생들을 각 7명씩 축구훈련군과 비교군으로 나누었다. 훈련군은 최대심박수의 62.7%의 강도로 1일에 30분씩의 축구훈련을 1주에 3일의 빈도로 12주 동안 실시하였다. 트레드밀에서의 런닝 중에 나타나는 심박수의 반응을 12주간의 훈련 준후에 각각 측정하였다 12주간의 축구훈련 후에 트레드밀 런닝의 각 단계에서 정신지체 남자 중학생의 심박수가 유의하게 감소했으며 특히 트레드밀에서의 런닝 5분 경과시에는 훈련 전 152bpm에서 훈련 후에는 129.8bpm으로 현격한 감소를 나타냈다. 최대심박수의 50-70% 정도의 강도로 훈련을 실시하는 것이 정신지체 남자중학생들에게는 안전하고 효율적인 것으로 나타났다. The purpose of this study was to identify the effects of 12 weeks of soccer training. on heart rate in mentally retarded children. Fourteen untrained middle school boys were randomly selected and divided into 2 groups, exercise group(N=7) and non-exercise group(N=7). The exercise group had 30 minute-soccer training 3 days a week with intensity of 62.7% of HR? for 12 weeks. The heart rate responses to treadmill running were measured before and after 12 weeks of soccer training. The data were tested(p<.05) with paired t-test and independent t-test. The heart rate responses were improved significantly when mentally retarded boys were subjected to 12 weeks of soccer training. It is recommended to use exercise intensity within moderated range (50%-70% HR?) for mentally retarded boys.

미약한 경부기종에 의해 발생된 개의 부분적 기관협착

이성호,박영석,박병권,김병수,정지윤,박창식,김상기 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10

A 10-year old male Pomerarian was presented with about a ten day history of stridor and severe paroxysmal honkinglike coughing which were aggravated with excitement and exercise. Auscultation revealed prominent inspiratory and expiratory crackles and wheezes over the caudal cervical trachea which were heard equally over both side of the chest, and a hemogram showed mild leucocytosis with mild dehydration. On the lateral radiographs of the neck and thorax, the cervical trachea was displaced ventrally by the air trapped between fascial planes of the cervical area near the thoracic inlet. It also revealed a marked narrowing of the tracheal lumen extending from the level of the fifth cervical to the second thoracic vertebra, and the lung field was judged to be within normal limits. There was no evidence of pneumomediastinum. A diagnosis of segmental tracheal stenosis was made. The emphysema of the retrotracheal area was thought to be a traumatic origin. The dog was treated successfully with strict cage confinement for a week. The tracheal stenosis caused by mild emphysema between the soft tissue of the cirvical area or mediastinum in the dog is very rare.

한국에서 동물간호사제도의 필요성과 자격시험에 대한 분석 및 평가

이성호,박영석,박병권,곽명순,강종일,조성진,김선균,김병수 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10

The study examined qualification test of the 1st term Animal Nurse Technician and conducted questionnaire survey as well as site investigation to settle Animal Nurse Technician system in Korea. The objects were undergraduates, graduates and director of animal hospital. 115 applicants applied to the qualification test of animal specialists, and 50 applicants passed the test to record 43.47% of ratio of successful applicants. The subjects having relative difficulty were anatomy physiology and public hygiene to record 19 points in average: In particular, the anatomy physiology recorded 35 points of the highest score and 6 points of the lowest score to have the highest deviation and to require adjustment of difficulties. 89.2% of the interviewee supported needs of animal nurse technician system in Korea to show positive response. At the survey of actual conditions of recruitment of animal nurse technicians at animal hospitals, 28.6% of the interviewee was employing the technicians, and 35.7% had employment plan, and 32.1% wanted to employ them under good conditions. In other words, 96 4% of the interviewee responded positively, while no more than 3.6% did negatively. In Korea, the animal nurse technicians had roles as follows: The animal hospitals gave priority to reception service and customer controls followed by medical examination support,whilethecollegesdidtomedicalexaminationsupport. To differentiate hospitals in accordance with the increase of number of animal hospitals, the animal hospitals should employ animal nurse technicians having specialty and qualifications on animal nurse technician examination. In particular, the roles and demand of animal nurse technicians were expected to grow because of developed technologies of veterinary medical science, development of medical supplies and instrument, and the increase of aged animals, etc. Therefore, job analysis on animal nurse technicians should be done to adjust the subjects of qualification test and to develop questions.

암환자의 혈청 중 Putrescine, Spermidine 및 Spermine의 분리 정량

신태용,이석봉,김대근,채병숙 우석대학교 의약품개발연구소 1997 藥學硏究誌 Vol.2 No.-

A high performance liquid chromatographic method developed for the determination of polyamines(putrescine, spermidine and spermine) in the serum of normal human and cancer patients. The benzoyi chloride derivatives of polyamines are separated on a uBondapak C_18 reverse-phase column with methanol/water(50v/50v) as the mobile phase. The results show that the mean level of polyamines in concer patients serum is much higher than that in normal human serum.

유한요소법에 의한 헬리컬기어의 맞물림 강도특성에 관한 연구

박성환,유용석,김병석,최명진,오택열 慶熙大學校 大學院 1993 高凰論集 Vol.12 No.-

In this study, the mesh stiffness characteristics of helical gears are evaluated by applying the finite element method. The bending and shear deflections on the contact line are obtained by the finite element method, and the contact deflections are obtained using the equation of Weber and Banaschek. Finally, using the influence method, contact ratio and load sharing ratio, the mesh stiffness characteristics of helical gears are evaluated. The results obtained could be summarized as follows: 1) By increasing the facewidth and helix angle of helical gears, a more constant mesh stiffness and transmission errors are obtained. 2) m_p+m_f〈2.0 The characteristics of this class helical gear pair are similar to that of spur gear. 3) m_p+ 1.0m_f≥2.0 This class helical gear pair gives a good performance as expected from helical gears.