Comparison of Proliferative Activity in Each Histological Subtypes of Benign and Atypical Intracranial Meningiomas by PCNA and Ki-67 Immunolabeling

최승진,장은덕,권성오,계대곤,박춘근,이상원,강준기,Choi, Seung Jin,Chang, Eun Deok,Kwon, Seung Oh,Kye, Dae Kon,Park, Choon Keun,Lee, Sang Won,Kang, Joon Ki The Korean Neurosurgical Society 2000 Journal of Korean neurosurgical society Vol.29 No.9

목 적 : 양성 뇌수막종에 비하여 이형성 및 악성 뇌수막종이 나쁜 임상적인 예후 및 양상을 보이는 것은 잘 알려져 있으나, 양성 뇌수막종에 있어서 각각의 병리조직학적 아형에 따른 생물학적 양상의 차이에 대해서는 잘 알려지지 않거나 일부 논란이 되고있다. 본 연구에서는 이형성 뇌수막종 및 양성 뇌수막종의 각각의 병리조직학적 아형에 따른 증식능의 차이여부를 알아보고자 PCNA와 Ki-67표지지수를 분석하였다. 방 법 : 본원에서 뇌수막종으로 수술을 시행하여 얻은, 재발을 보여 재수술을 시행한 2례를 포함하여, 파라핀에 포매시킨 27개의 조직을 대상으로 병리학적인 증식능을 분석하기 위해, PCNA에 대한 단일항체 및 MIB-1 단일항체를 이용한 면역조직화학적 염색을 시행하였다. 조직학적 분류상 meningothelial type이 8례, transitional type이 9례, fibroblastic type이 5례였으며, 이형성 수막종이 5례였다. 결 과 : PCNA표지지수의 평균값은 양성 수막종에서 meningothelial type이 $4.82{\pm}5.10%$, transitional type이 $9.01{\pm}4.25%$, fibroblastic type이 $5.66{\pm}5.32%$를 보였으나 이형성 수막종에서는 $27.62{\pm}19.67%$의 높은 지수를 나타냈고, Ki-67 표지지수의 평균값은 양성 수막종의 아형에서 각각 $0.43{\pm}0.85%$, $0.44{\pm}1.08%$, $0.24{\pm}0.18%$를 보이고, 역시 이형성 수막종에서는 $0.84{\pm}0.59%$의 높은 지수를 보였다. 즉, 양성 수막종에서 각각의 아형에 따른 PCNA 및 Ki-67 표지지수는 통계학적으로 의미있는 차이는 없었으나(p>0.05), 이형성 수막종에서는 의미있는 높은 표지지수를 보여(p<0.05) 양성 수막종에서 보다 높은 증식능을 보임을 알 수 있었다. 결 론 : PCNA 및 Ki-67 표지지수를 이용한 증식능의 비교결과, 양성 뇌수막종에서는 각각의 아형에 따른 생물학적 양상이나 예후는 차이가 없을것으로 생각되나, 이형성 수막종에서는 높은 증식능을 보여 이에 대한 예후를 예상할 수 있을것으로 생각되며, 또한 이러한 표지지수가 병리조직학적으로 양성과 이형성의 감별에 많은 도움이 될것으로 사료된다. Objective : The clinical prognosis and biological behavior of atypical and especially malignant meningiomas are well known to be worse than benign meningioma, but the degree of biological aggressiveness in each classical subtypes of benign meningioma is controversy. This study was performed to see whether there is a difference in the proliferative activity between each different histological subtypes of benign meningioma as well as atypical meningioma. Methods : Paraffin-embedded surgical specimens of 27 meningiomas, including two recurrent tumors, were studied to evaluate proliferative activity by immunohistochemical method with monoclonal antibodies to proliferating cell nuclear antigen(PCNA) and MIB-1. The specimens consisted of 8 cases of meningothelial, 9 cases of transitional, 5 cases of fibroblastic subtypes and 5 cases of atypical meningiomas. Results : Mean PCNA labeling indices of meningothelial, transitional and fibroblastic meningiomas were $4.82{\pm}5.10%$, $9.01{\pm}4.25%$ and $5.66{\pm}5.32%$, but that of atypical meningiomas was $27.62{\pm}19.67%$, noting a higher value compared to all three subtypes of benign meningiomas. Mean Ki-67 labeling indices of the above 3 subtypes were $0.43{\pm}0.85%$, $0.44{\pm}1.08%$ and $0.24{\pm}0.18%$, and that of atypical meningiomas was also revealed to be of higher value ($0.84{\pm}0.59%$). PCNA and Ki-67 labeling indices were not statistically different between histological subtypes of benign meningioma(p>0.05), but the differences of both immunolabeling between benign and atypical meningiomas were statistically significant(p<0.05). Conclusion : Immunolabeling of PCNA and Ki-67 in intracranial meningiomas reveals no prognostic difference between meningothelial, transitional and fibroblastic subtypes in classical benign meningiomas by measuring expression of PCNA and Ki-67, but it seems to be helpful in differentiating benign and atypical meningioma, later showing more proliferative activity and biological aggressiveness.

광주광역시 도시기능의 공간분포패턴에 관한 연구

권준오,원열,강기수 조선대학교 국토개발연구소 1997 국토개발연구 Vol.17 No.1

This study has been carried out in order to offer basic data necessary to effective planning in space structure of city by examining the concentration degree of city functions in administrative parts through analyzing space distribution of Kwangju City. This study has been resulted in ; First, service works are mostly distributed in down areas(43.2%), and particularly high in Dong-Ku which has least space for administrative part and Seo-Ku, Puk-Ku and Kwangsan-Ku was in order. It showed that the functions of the city is preponderated. Second, the area with high rate of population allotment is relatively far from downtown areas and one with high rate of indusrtrial allotment is relatively near from downtown areas. It showed us the internal space structure in Kwangju shaping a concentric circle. Third, the following areas show specialization in particular works ; ⓐ Ariculture; Tae-Ye, Ssang-Chon2, Nong-Seong, ⓑ Mining Industry; Tae-Kum, ⓒ Manufacturing Industry; Ha-Nam, You-Deuk, Bon-Chon, ⓓ Elecrical Industry; Yong-Bong, Seo-San, So-Chon, ⓔ Construction; Tae-Kum, ⓕ Wholesacle and detail; Chung-Kum, Tae-Kum, ⓖ Transportation business; Shin-An, Tae-Kum, ⓗ Financial business; Chung-Kum, Tae-Kum, ⓘ Social works; Seo-Seuk, Fourth, manufacturing industry was concentrated in industrial complex area and transportation industry in the area of gateway to Kwangju and social works in the area of educational facilities respectively.

백두대간 관리범위 설정에 관한 연구(Ⅱ) : 준산악형 구간을 대상으로 In the Case of Semi-mountainous District

권태호,최송현,유기준 한국지리정보학회 2004 한국지리정보학회지 Vol.7 No.1

백두대간은 국토의 근간이자 중요한 생태축이다. 그러나 최근 백두대간 지역을 대상으로 한 각종 개발요구가 증가함에 따라 이에 적절히 대응하기 위한 보전 전략 수립과 체계적인 관리의 필요성이 부각되고 있다. 이 연구는 백두대간 지역을 효율적으로 보호하기 위한 관리구역 설정을 제안하고자 수행하였다. 접근 방법은 백두대간의 적절한 관리범위 설정을 위해 수계 및 유역을 바탕으로 인간 간섭의 영향정도를 파악하는 것으로, 수치지도, Landsat TM 영상 등이 사용되었다. 준산악지형의 특성을 갖는 남덕유산~소사고개 구간을 대상으로 유역확장방식을 사용하여 유역차수에 따른 경작지, 도시, 나지, 수계, 산림 등의 토지이용상태의 변화를 추적하였다. 그 결과 유역확장방식은 백두대간 관리범위 설정기법으로 효율적이었으며, 관리범위는 마루금을 중심으로 무주(8차)와 거창 (5차) 방향이 상이하게 설정되었다. Baekdu-daegan is the greatest mountain chain as well as the major ecological axis of the Korean Peninsula. In recent year, however, this area is faced with the various kinds of developmental urge. To cope adequately with these problems, this study was executed to prepare synthetic and systematic management with conservation-oriented strategy for Baekdu-daegan and to suggest spatially definite zoning for the managerial area. This study is to take into consideration the traditional concepts of stream and watershed as well as the actual disturbance on Baekdu-daegan area. The study area is selected with semi-mountainous type, from Namdeokyusan to Sosagogae. To propose the process for reasonably establishing the managerial boundary adjacent to the ridges, the analysis was carried out that ArcGIS was mainly used for its analysis with digital maps, Landsat TM image and ArcGIS Hydro Model. Landsat TM image was classified by 5 land use types such as cultivated land, urban area, barren area, water body and forest. Based on these analyses results, the managerial boundaries as alternatives from the Ridges were produced by watershed expansion process, and used for tracing the changes of areal ratio of various land use types to the relevant watersheds to search out the adequate managerial boundary. The results show that watershed expansion process could be effective tool for establishing the managerial boundary, and eighth expanded watershed toward Muju-Gun(west) and fifth expanded watershed toward Geochang-Gun (east) might be included for the adequate managerial boundary of the case site.

방사선 조사선량에 따른 백서 악하선의 caspase-3 발현양상

권기정,최용석,황의환,이상래,고광준 대한구강악안면방사선학회 2006 Imaging Science in Dentistry Vol.37 No.1

Purpose : To investigate the caspase-3 expression in the acinar and ductal cells of rat submandibular glands after the irradiation of various doses. Materials and Methods: The male Sprague-Dawley rats weighing approximately 250 gm were used for this study. The experimental group was irradiated with a single absorbed dose of 2, 5, 10, and 15 Gy on the head and neck region. The rats were sacrificed on the 1st, 3rd, 7th, 14th, 21st, and 28th day after irradiation. The specimens including the submandibular gland were sectioned and observed using histopathological and immunohistochemical methods. Results : The local destruction of the acinar and ductal cells and the karyopyknotic nuclei of the acinar cells were observed in the 2 Gy and 5 Gy irradiation groups later than in the 10 Gy and 15 Gy irradiation groups. And the expression of caspase-3 was prominent only in the ductal cells in the 2 Gy and 5 Gy irradiation groups. Conclusion : This experiment suggests that radiation-induced apoptosis in the ductal cells of rat submandibular glands was induced by a low dose radiation associated with the activation of caspase-3 and radiation-induced necrosis was induced by a high dose radiation. (Korean J Oral Maxillofac Radiol 2006; 36 : 7-15)

중추성 요붕증으로 발현된 랑거한스세포 조직구증식증 1례

김준성,윤원상,김근모,최영권,박기원,김병희,유용상 대한소아내분비학회 2004 Annals of Pediatirc Endocrinology & Metabolism Vol.9 No.1

인공 연못의 부영양화 원인 및 저감대책 : - 현충사 경내 연못을 중심으로 -

장봉기,권용순,박종안,이종화,허준무 순천향대학교 기초과학연구소 2001 순천향자연과학연구 논문집 Vol.7 No.1

The field investigation including survey of water quality and sediment was conducted to evaluate trophic level and to find out appropriate control measures of artificially eutrophicated pond. The pond is located in the Hyunchoongsa, Asan, and has been a major water quality problem which has received considerable attention over the last decades because of accelerated aging of pond waters may be resulting from elevated loading of nutrients. Comprehensive survey of water quality and bottom sediment was carried out for one year to find out history of general characteristics, from November 1999 to July 2000. Average concentration of total nitrogen and phosphorus in the pond water was 25.43mg/ℓ and 0.2mg/ℓ respectively, and other characteristics showed higher trophic level. Chlorophyll-a concentration was 24.43mg/ℓ, and major group of algae was green algae of Closterim, which occupied 97.7%. The eutrophication rate index of one-year average was 70.91, and this level meant the pond was severe eutrophication state. Higher concentration of nutrient was detected in sediment of the pond, which was 2,717.06mg/ℓ and 83.20mg/ℓ, and these estimated values also meant extreme eutrophication state of the pond. Inflowing water quality was also monitored to find out the nutrient source. Results showed that inflowing water was not good for the supplying water for the pond since Total nitrogen and phosphorus concentration of inflowing water were 28.87mg/ℓ and 0.25mg/ℓ, respectively, and these nutrient concentrations were very high leading to algae bloom of the pond. Therefore it was concluded that supply of clean water is good for remedial actions to the pond, and flushing is a appropriate measures to control this eutrophicated pond. Simulation using modified simple model showed the trophic level of the pond will be change to water quality level of lake and pond I after clean water supply of 356m3/day for sixty-days.

한국産 황어의 종묘생산에 관한 연구 1. 産卵習性 및 初期生活史

조재권,오성현,노병율,한경호,성기백,박준택 麗水大學校 産業基術硏究所 1999 産業基術硏究所 論文集 Vol.8 No.-

강원도 양양군에 위치한 남대천과 전라남도 섬진강에 분포하고 있는 황어의 종료생산을 위한 기초자료로 産卵習性과 初期生活史에 관하여 관찰한 결과는 다음과 같다. 황어의 産卵期는 자연상태에서 3∼6月이고, 産卵은 흐르는 하천의 자갈 밑에 하였으며, 受精된 卵은 작은 자갈의 표면에 붙어있었다. 알의 크기는 2.70∼3.20㎜(평균 3.00㎜)로 油球는 없었다. 孵化는 평균 사육수온 10.60℃에서 受精 後 300시간부터 시작되었으며, 312시간만에 완료되었다. 孵化 直後의 仔魚는 전장 6.35∼6.87㎜(평균 10.67㎜)로 난황이 거의 흡수되어 後期仔魚期로 접어들며, 입과 항문이 열려있었다. 孵化 後 7∼9일째 後期仔魚는 전장 11.35∼12.30㎜(평균 11.65㎜)로 등지느러미가 생길 부분이 융기하기 시작하였다. 孵化 後 12∼13일째 개체는 전장 12.65∼13.15㎜(평균 12.80)로 이때부터 Daphnia sp.와 Brachionus plicatilis를 攝餌하였으며, 성장이 다소 빨라졌고, 脊索의 끝부분은 45°위로 굽어져 있었다. 孵化 後 43∼45일째의 개체는 전장이 19.55∼22.85㎜(평균 21.95㎜)로 모든 지느러미 줄기가 정수에 달하여 稚魚期로 이행하였다. 孵化 後 50∼52일째의 稚魚들은 전장이 21.54∼27.35㎜(평균 24.55㎜)로 몸의 형태, 체색 및 옆줄이 완전하게 형성되어 成魚에 닮아 있었다. The spawning behavior and early life history of sea rundace, Tribolodon hakonensis were studied. Spawning of T. hakonensis occurs enmasse on bottoms of stream with a spawning season of March to June. The eggs are attached to the under surface of the gravel. The eggs riped were demersal and adhesive, and their diameter were 2.70∼3.20㎜(mean 3.00㎜). Hatching was started at 300 hours after fertilization at mean water temperature 10.60℃. Newly-hatched larvae were 6.35∼6.87㎜ in total length(TL, Mean 6.65㎜), and mouth and anus were not yet opened. 4∼5 days old larvae transformed to postlarva stage and they were 10.25㎜∼11.15㎜ in TL (mean 10.67㎜). As yolk sac were nearly absorbed, mouth and anus were opened. In 7∼9 days after hatching, total length 11.35∼12.30㎜(mean 11.65㎜). The part of the fin-fold at dorsal fin became high. 12∼13 days old larvae were 12.65∼13.15㎜ in TL(mean 12.80㎜), and they fed Daphnia sp. and Brachionus plicatilis actively. In this time the growth rate slightly increased and tip of notocord was flesed on upward. Aggregate numbers of all fin rays were completed at over 19.55∼22.85㎜ in TL(43∼45 days after hatching), at which time the larvae reached the juvenile stage. 50∼52 days old larvae(21.54∼27.35㎜ in TL) were similar in body from, lateral line and color with adult.

하악에 전이된 위 선양암종

이지운,권기정,안현숙,고광준 대한구강악안면방사선학회 2005 Imaging Science in Dentistry Vol.35 No.3

Metastatic tumors to the jaw bones are uncommon. The most common metastatic tumors to the jaw bones are the breast, lung and kidney. In the jaw bones, the common location of the lesions is the mandible, and the posterior area of the mandible is more commonly affected. The radiographic appearance is quite variable. In this report, a very rare case of metastatic gastric adenocarcinoma to the mandible is presented. The patient had undergone a gastrectomy 3 years ago.

MC3T3-E1 조골세포주의 osteonectin과 bone sialoprotein mRNA 발현에 미치는 방사선의 영향

하쌍용,강기현,이상래,권기정,고광준 대한구강악안면방사선학회 2004 Imaging Science in Dentistry Vol.34 No.2

Purpose : To investigate the effects of irradiation on the phenotypic expression of the MC3T3-E1 osteoblastic cell line, especially on the osteonectin and bone sialoprotein. Materials and Methods : Cells were irradiated with a single dose of 0.5, 1, 4 and 8 Gy at a dose rate of 5.38 Gy/min using Cs-137 irradiator. After specimens were harvested, total RNA was extracted on the 3rd, 7th, 14th, 21st day after irradiation. The total RNA was reverse-transcribed and the resulting cDNAs were subjected to amplification by PCR with a pair of primers. Results : The irradiated cells showed a dose-dependent increase in osteonectin mRNA expression when compared with the unirradiated control group. The irradiated cells showed no difference in bone sialoprotein mRNA expression when compared with the unirradiated control group. In accordance with the duration of culture period after irradiation, the level of osteonectin mRNA expression showed no difference, but it increased a little at the 21st day in the 4 and 8 Gy exposure groups. In the case of bone sialoprotein, however, the level of mRNA expression increased significantly at the 3rd and 7th day after irradiation, but it showed no difference at the 14th and 21st day when compared with the control group. Conclusion : These results showed that each single dose of 0.5, 1, 4 and 8 Gy influenced the mRNA expression of osteonectin and bone sialoprotein at the calcification stage of osteoblastic cells, suggesting that single dose of irradiation affected the osteoblastic bone formation at the cell level.

서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1996)

김재홍,황동규,전재홍,김윤석,김중환,김용준,이창균,임동진,김현수,조창근,김경문,박상훈,전우형,김희성,이호정,차명수,김갑형,김형석,김석우,황지환,박병순,권오상,이민수,송기훈,성소영,이인섭,부태성 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2

Background : In recent years, gonorrhea has been panedemic and remains one of the most commom STDs in the world, especially in developing countries. Objective & Methods: For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results: In 1996, 139 strains of N. gonorrhoeae were isolated, among which 53(39.0%) were PPNG. Conclusion: Our results suggests that after a peak of 74.3% in 1993, the prevalence of PPNG in Seoul is gradually declining.