스테로이드로 유발된 쥐의 근골격계 변화에대한 성장호르몬의 치료 효과

최기섭,박창일,정우성,이상건,박용욱 大韓再活醫學會 2000 Annals of Rehabilitation Medicine Vol.24 No.5

흡연에 의한 급성 호산구성 폐렴 2예

박종빈,김학렬,주현준,유태양,신성남,신정현,송정섭,황기은,김소영,양세훈,정은택 圓光大學校 醫科學硏究所 2008 圓光醫科學 Vol.23 No.2

최근 흡연을 시작한 젊은 성언에서 급성 호산구성 폐렴의 발생에 대한 증례가 보고되고 있다. 급성 호산구성 폐렴은 대개 발열을 동반하기 때문에 초기에는 감염성 폐렴으로 잘못 진단, 치료되는 경우가 많다. 아직 정확한 병태 생리 및 조직학적 소견은 밝혀져 있지 않지만 병력 청취 및 임상 양상, 방사선 소견의 관찰을 통해 급성 호산구성 폐렴을 의심하고, 진단을 위해 기관지 폐포 세척액 검사를 시행한다면 좀 더 쉽게 진단 내릴 수 있을 것이다. 본 저자들은 최근 처음 시작한 흡연에 의해 발생한 급성 호산구성 폐렴을 진단하고, 스테로이드를 투여하여 성공적으로 치료한 환자 2예를 경험하였기에 이를 문헌 고찰과 함께 보고하는 바이다. Acute eosinophilic pneumonia(AEP) is characterized by acute febrile respiratory illness associated with diffuse pulmonary infiltration and pulmonary eosinophilia. The specific etiology for acute eosinophilic pneumonia is elusive. By some investigators, cigarette smoking is suggested as a causative substance which can cause AEP. In recent, the authors experienced two cases of AEP following cigarette smoking. Both cases had characteristic features including age around 20 years, new onset smoking before occurance of AEP, diffuse infiltration on chest radiography, pulmonary eosinophilia based on bronchoalveolar lavage and acute improvement after steroid therapy. These clinical features are resemble with previous smoking induced AEP case reports. Base on these clinical features, cigarette smoking associated AEP could be diagnosed more easily.

전자빔 증착법으로 제조된 박막 태양전지용 CuGaS₂ 박막의 특성

정운조 ( Woon-jo Jeong ),문인섭 ( In-seob Moon ),조순계 ( Soon-kye Cho ),김민기 ( Min-ki Kim ),김운섭 ( Woon-sub Kim ),부수일 ( Su-il Boo ),안호근 ( Ho-geun Ahn ),양현훈 ( Hyeon-hun Yang ),박계춘 ( Gye-choon Park ) 한국환경기술학회 2008 한국환경기술학회지 Vol.9 No.2

전자빔 증착법에 의해 70℃의 기판온도와 350℃에서 60분 동안 열처리한 경우 단상의 CuGaS₂ 박막이 얻어졌으며, 이때의 XRD 회절 피크는 회절각 28.8°에서 (112)방향으로 가장 강한 피크가 나타났고, 두 번째로 강한 피크는 회절각 49.1°에서 (204)방향을 나타났다. 또한 CuGaS₂ 박막의 격자상수 a와 c는 각각 5.37Å과 10.54Å이었다. 그리고 CuGaS₂ 박막의 그레인 사이즈는 최대 1μm 정도였다. 또한 황이 과잉 공급된 CuGaS₂ 박막의 (112) 피크가 황이 추가로 공급되지 않은 경우에 비해 약 10% 정도 더 강하게 나타남을 알 수 있었다. 그리고 이와 같은 제조된 CuGaS₂ 박막의전기저항률, 홀 이동도 및 캐리어 농도는 각각 1.4Ω-cm, 15㎠/V·sec and 2.9×10¹⁷cm^-3이었다. 본 연구에서 CuGaS₂ 박막의 저항률은 홀 이동도보다 캐리어 농도에 더 지배적임을 알 수 있었고, 상기와 같이 제조된 CuGaS₂ 박막은 모두 p-type 반도체 특성을 나타냈다. By EBE(Electron Beam Evaporation) method, Single phase CuGaS₂ thin film with the highest diffraction peak of (112) at diffraction angle(2θ) of 28.8˚ was made at substrate temperature of 70℃, annealing temperature of 350℃ and annealing time of 60min. And second highest (204) peak was shown at diffraction angle of (2θ) of 49.1˚. Lattice constant of a and c of that CuGaS₂ thin film was 5.37Å and 10.54Å respectively. The greatest grain size of the thin film was about 1㎛. The (112) peak of single phase of CuGaS₂ thin film at annealing temperature of 350℃ with excess S supply was appeared with a little higher about 10% than that of no excess S supply. And the resistivity, Hall mobility and carrier concentration at room temperature of p-type CuGaS₂ thin film was 1.4Ω-cm, 15㎠/V·sec and 2.9×10¹⁷cm^-3 respectively. It was known that carrier concentration had considerable effect than mobility on variety of resistivity of the fabricated CuGaS₂ thin film, and the polycrystalline CuGaS₂ thin films were made at these conditions were all p-type.

Regulation of toll-like receptors expression in muscle cells by exercise-induced stress

Park, Jeong-Woong,Kim, Kyung-Hwan,Choi, Joong-Kook,Park, Tae Sub,Song, Ki-Duk,Cho, Byung-Wook Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.10

Objective: This study investigates the expression patterns of toll-like receptors (TLRs) and intracellular mediators in horse muscle cells after exercise, and the relationship between TLRS expression in stressed horse muscle cells and immune cell migration toward them. Methods: The expression patterns of the TLRs (TLR2, TLR4, and TLR8) and downstream signaling pathway-related genes (myeloid differentiation primary response 88 [MYD88]; activating transcription factor 3 [ATF3]) are examined in horse tissues, and horse peripheral blood mononuclear cells (PBMCs), polymorphonuclear cells (PMNs) and muscles in response to exercise, using the quantitative reverse transcription-polymerase chain reaction (qPCR). Expressions of chemokine receptor genes, i.e., C-X-C motif chemokine receptor 2 (CXCR2) and C-C motif chemokine receptor 5 (CCR5), are studied in PBMCs and PMNs. A horse muscle cell line is developed by transfecting SV-T antigen into fetal muscle cells, followed by examination of muscle-specific genes. Horse muscle cells are treated with stressors, i.e., cortisol, hydrogen peroxide (H₂O₂), and heat, to mimic stress conditions in vitro, and the expression of TLR4 and TLR8 are examined in stressed muscle cells, in addition to migration activity of PBMCs toward stressed muscle cells. Results: The qPCR revealed that TLR4 message was expressed in cerebrum, cerebellum, thymus, lung, liver, kidney, and muscle, whereas TLR8 expressed in thymus, lung, and kidney, while TLR2 expressed in thymus, lung, and kidney. Expressions of TLRs, i.e., TLR4 and TLR8, and mediators, i.e., MYD88 and ATF3, were upregulated in muscle, PBMCs and PMNs in response to exercise. Expressions of CXCR2 and CCR5 were also upregulated in PBMCs and PMNs after exercise. In the muscle cell line, TLR4 and TLR8 expressions were upregulated when cells were treated with stressors such as cortisol, H₂O₂, and heat. Migration of PBMCs toward stressed muscle cells was increased by exercise and oxidative stresses, and combinations of these. Treatment with methylsulfonylmethane (MSM), an antioxidant on stressed muscle cells, reduced migration of PBMCs toward stressed muscle cells. Conclusion: In this study, we have successfully cultured horse skeletal muscle cells, isolated horse PBMCs, and established an in vitro system for studying stress-related gene expressions and function. Expression of TLR4, TLR8, CXCR2, and CCR5 in horse muscle cells was higher in response to stressors such as cortisol, H₂O₂, and heat, or combinations of these. In addition, migration of PBMCs toward muscle cells was increased when muscle cells were under stress, but inhibition of reactive oxygen species by MSM modulated migratory activity of PBMCs to stressed muscle cells. Further study is necessary to investigate the biological function(s) of the TLR gene family in horse muscle cells.

막과 AOP 공정을 이용한 난분해성 산업폐수 재이용 연구

김성준 ( Sung-joon Kim ),김명희 ( Ming-ji Jin ),전용태 ( Yong-tae Jeon ),정태섭 ( Tae-sub Jeong ),박영기 ( Young-ki Park ),원찬희 ( Chan-hee Won ) 한국환경기술학회 2012 한국환경기술학회지 Vol.13 No.1

본 연구는 UV, O₃, 촉매로 이루어진 물 재이용 시스템을 연구하였고 본 시스템 실플랜트를 제작하여 실험을 실시하였다. 이 시스템은 하이브리드 방식으로 막기술과 산화과정을 조합하였으며 폐수종말처리장 유출수에 적용한 결과 저비용 고효율의 효과를 얻을 수 있었다. 대상폐수의 성상과 재이용 하고자 하는 양에 따라 UV와 O₃량 등을 변경 가능토록 설계되었으며 8주동안 본 시스템을 운전한 결과 SS, BOD₅, COD_Cr, COD_Mn, T-N, T-P, 색도의 처리효율은 각각 92, 92, 90, 90, 70, 63, 90%를 보였고 총대장균과 일반세균은 모두 제거되었다. 환경부의 용도별 하수처리수 재이용 수질권고기준의 공업용수기준을 충분히 만족하고 있어 본 시스템이 산업폐수의 재이용을 위한 폐수 재이용 처리시스템으로 충분히 활용 가능하다는 결론을 얻었다. 추가적으로 본시스템으로 잘 처리가 되지 않은 인 혼합물의 처리를 위한 연구가 필요할 것이다. In this study, the water reuse system consisting of UV, O₃, and catalyst was developed and its performance was evaluated using a pilot plant in the treatment of the discharged water from the existing water treatment plant. Our system adopted the hybrid system, the combination of membrane technology and advance oxidation process, which results in low operation cost and high efficiency in the treatment of wastewater. The unique feature of our process is that the system was designed to easily adjust the operational conditions such as the amount of feeding UV and O₃ based on the quaility of target water. During the operation of pilot plant for 8 weeks, the average removal efficiencies for SS, BOD₅, COD_Cr, COD_Mn, T-N, T-P, and color were investigated to be 92, 92, 90, 90, 70, 63 and 90%, respectively. No bacteria were detected during the operation of the pilot plant. Consequently the quality of treated water was satisfied with the standard for water reuse issued by Ministry of Environment of Korea. It means that the treated water from our water process could be re-supplied as industrial water. The results obtained from our lab and pilot scale experiments showed sufficient evidence to prove that the treated wastewater from our system was good enough to be reused for the purpose of industrial water supply usage. More researches are necessary in the future to treat phosphoric compounds which were not effectively treated in out process.

호안블록용 박테리아 기반 악취저감 키트의 악취제거 성능평가

양근혁,문주현,정기태,윤현섭,심재일,Keun-Hyoek Yang,Ju-Hyun Mun,Ki-Tae Jeong,Hyun-Sub Yoon,Jae-Il Sim 한국건설순환자원학회 2024 한국건설순환자원학회 논문집 Vol.12 No.2

이 연구에서는 박테리아 기반 악취저감 키트의 악취제거 성능을 평가하였다. 박테리아는 악취오염원인 암모니아(NH₃), 황화수소(H₂S), 총질소(T-P) 및 총인(T-N)을 제거할 수 있는 Rhodobacter capsulatus, Paracoccus limosus 및 Brevibacterium hankyongi를 사용하였다. 사용된 소재들은 박테리아와 다공성골재(팽창질석, 제올라이트 비드, 활성탄)이며, 제거 메커니즘에 따라 소재들의 융합을 달리하였다. 물리적 흡착 메커니즘을 갖는 소재들(제올라이트 비드 및 활성탄)은 악취오염원(NH₃, H₂S, T-P 및 T-N)의 농도 저감율이 점차 둔화되어 48시간 이후부터 더 이상의 악취오염원의 농도의 저감 효과가 없었다. 생물학적 흡착 메커니즘으로 악취를 제거하는 박테리아가 고정화된 팽창질석은 지속적인 농도 저감으로 108시간 이후에 악취오염원의 농도가 0 ppm에 도달하였다. 결과적으로 실제 하천수에서 물리적 흡착 메커니즘을 갖는 소재들의 악취제거 성능은 환경부에서 제시하고 있는 악취 배출허용 기준을 만족하지 못한 반면, 박테리아가 고정화된 팽창질석은 악취 배출허용 기준을 만족하였으며, 수질도 1등급으로 평가되었다. This study evaluated the odor removal performance of a bacteria-based odor reduction kit. The bacteria used were Rhodobacter capsulatus, Paracoccus limosus, and Brevibacterium hankyongi, which can remove ammonia (NH₃), hydrogen sulfide (H₂S), total nitrogen (T-P), and total phosphorus (T-N), which are odor pollutants. The materials used were bacteria and porous aggregates (expanded vermiculite, zeolite beads, activated carbon), and the combination of the materials varied depending on the removal mechanism. Materials with a physical adsorption mechanism (zeolite beads and activated carbon) gradually slowed down the concentration reduction rate of odor pollutants (NH₃, H₂S, T-P, and T-N), and had no further effect on reducing the concentration of odor pollutants after 60 hours. Expanded vermiculite, in which bacteria that remove odors through a bio-adsorption mechanism were immobilized, had a continuous decrease in concentration, and the concentration of odor pollutants reached 0 ppm after 108 hours. As a result, the odor removal performance of materials with physical adsorption mechanisms in actual river water did not meet the odor emission standard required by the Ministry of Environment, while the expanded vermiculite immobilized with bacteria satisfied the odor emission permissible standard and achieved water quality grade 1.

식물추출물 싸임화이트, 클로브버드, 계피, 라벤더, 레몬 유칼립투스 정유의 생태독성평가

유아선(Are-Sun You),최영웅(Young-Woong Choi),정미혜(Mi-Hye Jeong),홍순성(Soon-Seong Hong),박연기(Yeon-Ki Park),장희섭(Hui-Sub Jang),박재읍(Jae-Yup Park),박경훈(Kyung-Hun Park) 한국농약과학회 2011 농약과학회지 Vol.15 No.4

Environment-friendly agro-materials tend to be preferred to chemical insecticides recently. For this reason, many studies were conducted to develop environment-friendly insecticides containing natural materials. The purpose of this study was to assess ecotoxicity for 5 plant essential oils (Thyme white, Clove bud, Cassia, Lavender, Lemon eucalyptus) expected to prevent from pests and be used for agro-materials. Target species used to assess acute toxicity were aquatic invertebrate (Daphina magna), fish (Oryzias latipes), honeybee (Apis mellifera L.) and earthworm (Eisenia fetida). The EC50 value, toxicological responses of thyme white, clove bud, and cassia to Daphina magna were 2.5, 2.8, and 6.9 mg L<SUP>-1</SUP> respectively and these values were moderately toxic according to standard of USEPA. EC<SUB>50</SUB> of Lavender and lemon eucalyptus were >10 mg L<SUP>-1</SUP> then they were considered as slightly toxicity. In case of acute toxicity test to fish, LC<SUB>50</SUB> of thyme white and cassia were 6.7 and 7.5 mg L<SUP>-1</SUP> each other. The other plant essential oils indicated LC<SUB>50</SUB> >10 mg L<SUP>-1</SUP>. Acute contact and oral toxicity test to Honeybee were conducted. As a result, LD<SUB>50</SUB> of all essential oils were >100 ㎍ a.i. bee<SUP>-1</SUP> in both of tests. In case of acute toxicity test to earthworm, LC<SUB>50</SUB> of thyme white, clove bud, cassia, lavender, and lemon eucalyptus were 149,230, 743, 234, and 635 mg kg<SUP>-1</SUP>, respectively. In conclusion, if the safety for earthworm is confirmed, 5 plant essential oils are expected to be use for environment-friendly insecticide materials with low risk against ecosystem and contribute to developing environment-friendly agro-materials.

Self-Assembly of CdTe Nanoparticles Into Nanowires by a Specific Wavelength of Light.

Kim, Ki-Sub,Lee, Ki Sun,Kang, Jeong Won,Park, Byung Heung American Scientific Publishers 2015 Journal of Nanoscience and Nanotechnology Vol.15 No.7

<P>CdTe nanowires were synthesized from individual nanoparticles via self-assembly at a specific wavelength of light. The wavelength of 500 nm resulted in a self-assembly of nanoparticles into nanowires. Most of the produced nanowires were straight and long in shape and their length ranged from 300 nm to 20 µm. The oxidation of Te2- in CdTe nanoparticles under the visible light resulted in the assembly of nanowires consisting of several layers of individual nanoparticles. Transmission electron microscopy and scanning electron microscopy were performed to characterize the synthesized nanostructures. Energy-dispersive X-ray demonstrated the atomic percentage of nanowires. Photoluminescence showed that the wavelength of the nanostructures is slightly blue-shifted from 555 to 548 nm.</P>

RESEARCH ARTICLE : Role of metabolish by intestinal microbiota in pharmacokinetics of oral baicalin

( Mi Jeong Kang ),( Gyu Sub Ko ),( Do Gyeong Oh ),( Jin Sung Kim ),( Keumhan Noh ),( Wonku Kang ),( Won Ki Yoon ),( Hyoung Chin Kim ),( Hye Gwang Jeong ),( Tae Cheon Jeong ) 영남대학교 약품개발연구소 2014 영남대학교 약품개발연구소 연구업적집 Vol.24 No.0

Baicalin (baicalein-7-glucuronide) is a flavonoid purified from Scutellaria baicalensis Georgi that has traditionally been used for treatment of hypertension, cardiovascular diseases, and viral hepatitis. In this study, the effects of intestinal microbiota on the pharmacokinetics of baicalin were investigated in normal and antibiotic-pretreated rats following p.o. administration of 100 mg/kg baicalin by using liquid chromatography/ion trap mass spectrometry. When rats were pretreated orally with cefadroxil, oxytetracycline and erythromycin for 3 days to control the number of intestinal bacteria, the pharmacokinetic parameters of oral baicalin were significantly affected by antibiotics: Cmax, T1/2(β), Kel and AUC values were significantly changed compared to those in normal rats. These results indicate that intestinal microbiota might play a key role in the oral pharmacokinetics of baicalin.

Role of metabolism by intestinal microbiota in pharmacokinetics of oral baicalin

Kang, Mi Jeong,Ko, Gyu Sub,Oh, Do Gyeong,Kim, Jin Sung,Noh, Keumhan,Kang, Wonku,Yoon, Won Ki,Kim, Hyoung Chin,Jeong, Hye Gwang,Jeong, Tae Cheon 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.3

Baicalin (baicalein-7-glucuronide) is a flavonoid purified from Scutellaria baicalensis Georgi that has traditionally been used for treatment of hypertension, cardiovascular diseases, and viral hepatitis. In this study, the effects of intestinal microbiota on the pharmacokinetics of baicalin were investigated in normal and antibiotic-pretreated rats following p.o. administration of 100 mg/kg baicalin by using liquid chromatography/ion trap mass spectrometry. When rats were pretreated orally with cefadroxil, oxytetracycline and erythromycin for 3 days to control the number of intestinal bacteria, the pharmacokinetic parameters of oral baicalin were significantly affected by antibiotics: $C_{max}$, $T_{1/2(\beta)}$, $K_{el}$ and AUC values were significantly changed compared to those in normal rats. These results indicate that intestinal microbiota might play a key role in the oral pharmacokinetics of baicalin.