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- 저자 : Keun Pill Kim (검색결과 24 건)
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불완전 전뇌 허혈후 소생의 백서 모델을 이용한 재관류 뇌혈류량과 허혈성 뇌조직 손상도와의 관계
서정필,송근정,황태식,정연권,신백효,김승호 大韓應急醫學會 1999 대한응급의학회지 Vol.10 No.2
Background: Experimental data indicate that low-flow reperfusion following prolonged cardiocirculatory arrest may aggravate early cerebral microcirculatory reperfusion disorders. We investigated the influence of cerebral reperfusion flow change to the ischemic histopathologic damage of brain tissue after incomplete forebrain ischemia in rats. Materials and method: Anesthetized Sprague-Dawley rats were undergone ligation of both internal carotid artery by microvascular clamp for 10 minutes. After release of the clamp, reperfusion was started with several different flow levels (0, 10, 20, 30, 50, and 100%) of internal carotid artery comparing to pre-clamping phase using flowmeter. After 15minutes of reperfusion, rat brains were prepared by perfusion-fixation with 3% formaldehyde. Under light microscopic examination of Hematoxylin-Eosin stained tissue slide, histopathologic damage was examined at cortex, putamen, and hippocampus regions. Categorical hisotopathologic damage scores were derived in each regions by manual counts of ischemic neurons. Result: The histopathologic damage scores were 0, 10.2±0.5, 7.6±1.5, 5.9±1.4, 5.0±2.8, 3.5±0.7, and 1.0±0.0 in control, 0, 10,20, 30, 50, and 100% reperfusion groups, respectively(p<0.05). Conclusion: Our results showed significant increment of brain histopathologic damage scores along with decreasing amount of cerebral reperfusion flow after incomplete forebrain ischemia. We believe restoration of reperfusion flow to pre-ischemic level would be a critical component in attenuation of brain ischemic damage.
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( Keun Pill Kim ),( Ji Hee Yu ),( Soo Min Park ),( Hyun Jo Koo ),( Choo Bong Hong ) 생화학분자생물학회 2011 BMB Reports Vol.44 No.12
There is a broad range of different small heat shock proteins (sHSPs) that have diverse structural and functional characteristics. To better understand the functional role of mitochondrial sHSP, NtHSP24.6 was expressed in Escherichia coli with a hexahistidine tag and purified. The protein was analyzed by non-denaturing PAGE, chemical cross-linking and size exclusion chromatography and the H6NtHSP24.6 protein was found to form a dimer in solution. The in vitro functional analysis of H6NtHSP24.6 using firefly luciferase and citrate synthase demonstrated that this protein displays typical molecular chaperone activity. When cell lysates of E. coli were heated after the addition of H6NtHSP24.6, a broad range of proteins from 10 to 160 kD in size remained in the soluble state. These results suggest that NtHSP24.6 forms a dimer and can function as a molecular chaperone to protect a diverse range of proteins from thermal aggregation. [BMB reports 2011; 44(12): 816-820]
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Flooding Stress-Induced Glycine-Rich RNA-Binding Protein from Nicotiana tabacum
이미옥,Keun Pill Kim,Byung-gee Kim,Ji-Sook Hahn,Choo Bong Hong 한국분자세포생물학회 2009 Molecules and cells Vol.27 No.1
A cDNA clone for a transcript preferentially expressed during an early phase of flooding was isolated from Nicotiana tabacum. Nucleotide sequencing of the cDNA clone identified an open reading frame that has high homology to the previously reported glycine-rich RNA-binding proteins. The open reading frame consists of 157 amino acids with an N-terminal RNA-recognition motif and a C-terminal glycine-rich domain, and thus the cDNA clone was designated as Nicotiana tabaccum glycine-rich RNA-binding protein-1 (NtGRP1). Expression of NtGRP1 was upregulated under flooding stress and also increased, but at much lower levels, under conditions of cold, drought, heat, high salt content, and abscisic acid treatment. RNA homopolymer-binding assay showed that NtGRP1 binds to all the RNA homopolymers tested with a higher affinity to poly r(G) and poly r(A) than to poly r(U) and poly r(C). Nucleic acid-binding assays showed that NtGRP1 binds to ssDNA, dsDNA, and mRNA. NtGRP1 suppressed expression of the fire luciferase gene in vitro, and the suppression of luciferase gene expression could be rescued by addition of oligonucleotides. Collectively, the data suggest NtGRP1 as a negative modulator of gene expression by binding to DNA or RNA in bulk that could be advantageous for plants in a stress condition like flooding.
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Establishment of Waternode Stations for Water Quality Monitoring by IP-USN
Kwak, Pill Jae,Kim, Seog Ku,Yun, Sang Leen,Kang, Sung Won,Lee, Hyun Dong,Lee, Joo Yeon,Ban, Hyo Jin,Yang, Keun Ho,Oh, Hee Jae,Kim, Young Sung Trans Tech Publications, Ltd. 2011 Materials science forum Vol.695 No.-
<P>Waternode station is a floating composite water quality monitoring device. The waternode station houses the multiple water quality measurement devices, control panel for wireless communication and solar cells for applying electric power. The floating devices are classified into general model with vertical dropping system and river model without vertical dropping system. The water quality measurement device measures pH, water temperature, conductivity, dissolved oxygen, turbidity and nitrate. And it measures all parameters simultaneously. The control panel is used for IP-USN system. USN (Ubiquitous Sensor Network) is a network system by water quality measurement devices. IP-USN is a USN system that identifies particular sensor connected to the internet. The data information is transferred by wireless modem based on TFB (Triple Frequency Band). Field application results showed that the waternode station is a reliable and cost-effective water quality monitoring solutions.</P>
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Development of Multiple Water Quality Measurement Devices
Kwak, Pill Jae,Kim, Seog Ku,Yun, Sang Leen,Kang, Sung Won,Lee, Hyun Dong,Yang, Keun Ho,Oh, Hee Jae,Kim, Young Sung Trans Tech Publications, Ltd. 2011 Materials science forum Vol.695 No.-
<P>The water quality measurement device that we developed measures pH, water temperature, conductivity, dissolved oxygen, turbidity and nitrate. And it measures all parameters simultaneously. The water resistant and screw packing technology also applied for improved mechanical reliability during water quality monitoring. A comparison between the performances of major company products (YSI, Hydrolab etc.) and this device don't provide a stark contrast. This device was verified through the KOREA’s Environmental Examination Methods. This device is offered reliable and cost-effective water quality monitoring solutions. Upgrades will be available and will include the technologies that are self-cleaning optical sensors with integrated wipers remove biofouling and maintain high data accuracy and optimal power management and built-in battery compartment extends in situ monitoring periods.</P>
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