Culture conditions of gametes and their evaluating systems in mouse /

박기상,송해범 대구대학교 생명과학연구소, 2003 생명과학연구 Vol.2 No.2

3차원 전산화단층촬영 영상을 이용한 안면 연조직 두께 계측의 임상적 유용성

정호걸,김기덕,한승호,허경석,이제범,박혁,최성호,김종관,박창서 대한구강악안면방사선학회 2006 Imaging Science in Dentistry Vol.36 No.2

Purpose : To evaluate clinical usefulness of facial soft tissue thickness measurement using 3D computed tomographic images. Materials and Methods : One cadaver that had sound facial soft tissues was chosen for the study. The cadaver was scanned with a Helical CT under following scanning protocols about slice thickness and table speed; 3 mm and 3 mm/sec, 5 mm and 5 mm/sec, 7 mm and 7 mm/sec. The acquired data were reconstructed 1.5, 2.5, 3.5 mm reconstruction interval respectively and the images were transferred to a personal computer. Using a program developed to measure facial soft tissue thickness in 3D image, the facial soft tissue thickness was measured. After the ten-time repeation of the measurement for ten times, repeated measure analysis of variance (ANOVA) was adopted to compare and analyze the measurements using the three scanning protocols. Comparison according to the areas was analyzed by Mann-Whitney test. Results : There were no statistically significant intraobserver differences in the measurements of the facial soft tissue thickness using the three scanning protocols (p>0.05). There were no statistically significant differences between measurements in the 3 mm slice thickness and those in the 5 mm, 7 mm slice thickness (p>0.05). There were statistical differences in the 14 of the total 30 measured points in the 5 mm slice thickness and 22 in the 7mm slice thickness. Conclusion : The facial soft tissue thickness measurement using 3D images of 7 mm slice thickness is acceptable clinically, but those of 5 mm slice thickness is recommended for the more accurate measurement.

3차원 전산화단층촬영 영상을 이용한 얼굴 연조직 두께 계측

정호걸,김기덕,한승호,신동원,허경석,이제범,박혁,박창서 대한구강악안면방사선학회 2006 Imaging Science in Dentistry Vol.37 No.1

Purpose : To evaluate accuracy and reliability of program to measure facial soft tissue thickness using 3D computed tomographic images by comparing with direct measurement. Materials and Methods : One cadaver was scanned with a Helical CT with 3 mm slice thickness and 3 mm/sec table speed. The acquired data was reconstructed with 1.5 mm reconstruction interval and the images were transferred to a personal computer. The facial soft tissue thickness were measured using a program developed newly in 3D image. For direct measurement, the cadaver was cut with a bone cutter and then a ruler was placed above the cut side, The procedure was followed by taking pictures of the facial soft tissues with a high-resolution digital camera. Then the measurements were done in the photographic images and repeated for ten times. A repeated measure analysis of variance was adopted to compare and analyze the measurements resulting from the two different methods. Comparison according to the areas was analyzed by Mann-Whitney test. Results : There were no statistically significant differences between the direct measurements and those using the 3D images (p>0.05). There were statistical differences in the measurements on 17 points but all the points except 2 points showed a mean difference of 0.5 mm or less. Conclusion : The developed software program to measure the facial soft tissue thickness using 3D images was so accurate that it allows to measure facial soft tissues thickness more easily in forensic science and anthropology. (Korean J Oral Maxillofac Radiol 2006; 36 : 49-54)

수영이 비만 여중생들의 혈중 렙틴농도, 지질수준과 체지방률과 미치는 영향

곽용순,김문희,김기범,박온서 한국스포츠리서치 2003 한국 스포츠 리서치 Vol.14 No.2

This study was to varify the effects of swimming on leptin level, lipid components (TC, TG, LDL-C, HDL-C) and percent body fat in girl's middle school students during 12 weeks. The subjects of study were divided into obese women group (n=9) and control group (n=9) randomly selected. Exercise intensity of swimming in this study was HRmax 50~80%, exercise duration 50min per day, exercise frequency during 12 weeks. Statistical method for data processing produced mean and SD of total variable. To analyze difference between groups and plasma variables level had executed 2×3 ANOVA. This study was given conclusion as follows: In exercise group, we showed that plasma HDL-C was increased but plasma leptin, TG and LDL-C were decreased during 12wks swimming. Also, %fat were decreased during 12wks swimming.

A New Sperm Preparation Method for Testicular Sperm Extraction - Intracytoplasmic Sperm Injection(TESE-ICSI) Cycles : Simple, Effective and Rapid Method

Park, Kee Sang,Park, Yoon Kyu,Song, Hai Bum,Lee, Taek Hoo,Chun, Sang Sik 경북대학교 병원 2003 경북대학교병원의학연구소논문집 Vol.7 No.1

The aim of this study was to recover spermatozoa easily from fresh or trozen-thawed testicular sperm extraction(TESE) samples. We simply minced and washed the TESE samples in medium. Spermatozoa were recovered and washed in the bottom of a PVP droplet with an injection pipette and injected into oocytes. In all cycles 100% of spermatozoa(motile and/or immotile) could be recovered. Injected oocytes were fertilized(100% per cycle and in 41.9% of oocytes) and fertilized oocytes were cleaved(100% per cycle; 38.5% of injected oocytes; 91.8% of fertilized oocytes) after ICSI with fresh or frozen-thawed TESE spermatozoa. The rates of progression to the embryo stage(2-8 cell) and blastocyst formation and their quality were similar in both the fresh sample group and the frozen-thawed sample group. Both of embryo transfer(73.5%) and embryo-blastocyst transfer(26.5%) were performed successfully. Thirty-four cycles of ICSI with testicular spermatozoa resulted in 23.5% pregnancy rates(fresh sample group, 22.2%; frozen-thawed sample group, 24%) with both embryo transfer(8%) and embryo-blastocyst transfer(66.7%). This new sperm preparation method is very simple, easy, effective and rapid for recovering spermatozoa from TESE samples for ICSI.

Reference ranges for autonomic function tests in healthy korean adults

Park, Kee Hong,Kim, Byoung Joon,Kang, Sa-Yoon,Oh, Sun-Young,Sohn, Eun Hee,Song, Kyeong-jin,Shin, Jin-Hong,Kang, Kyoung Hwa,Cho, Eun Bin,Jeong, Heejeong,Lee, Hyung,Kim, Hyun Ah,Kim, Rock Bum,Park, Ki-J The Korean Society of Clinical Neurophysiology 2019 Annals of Clinical Neurophysiology Vol.21 No.2

Background: The standardized autonomic function test has become widely available. However, there are no reference data for this test for the Korean population. This study explored reference data for sudomotor and cardiovagal function tests for the Korean population. Methods: The sweat volume by quantitative sudomotor axon reflex test, heart-rate response to deep breathing (HRdb), expiration:inspiration (E:I) ratio, and Valsalva ratio (VR) were measured in 297 healthy Korean volunteers aged from 20 to 69 years. Multivariate regression analysis was performed to evaluate the effects of age, sex, and body mass index on these variables. The 2.5th, 5th, 10th, 90th, 95th, and 97.5th percentile values were obtained for each investigation. Results: The sweat volume was higher in males than in females. The HRdb and E:I ratio were negatively correlated with age, and were higher in males than in females. The VR was negatively correlated with age, but it was not correlated with sex. Conclusions: This study has provided data on the reference ranges for sudomotor and cardiovagal function tests in healthy Korean adults.

Is High-Dose Nafamostat Mesilate Effective for the Prevention of Post-ERCP Pancreatitis, Especially in High-Risk Patients?

Park, Kee Tae,Kang, Dae Hwan,Choi, Cheol Woong,Cho, Mong,Park, Su Bum,Kim, Hyung Wook,Kim, Dong Uk,Chung, Chung Wook,Yoon, Ki Tae Lippincott Williams Wilkins, Inc. 2011 PANCREAS Vol.40 No.8

OBJECTIVE:: Infusion of the protease inhibitor nafamostat mesilate (20 mg) effectively prevents post-ERCP pancreatitis, but only in low-risk groups. This study was performed to evaluate the use of high-dose nafamostat mesilate (50 mg) for prevention of post-ERCP pancreatitis (PEP), especially in high-risk groups. METHODS:: A total of 608 patients who underwent ERCP were included; 13 patients were excluded. Patients were divided into 3 groups: controls (group A), infusion with 20 mg of nafamostat mesilate (group B), or infusion with 50 mg of nafamostat mesilate (group C). The incidence of PEP was analyzed. RESULTS:: The overall incidence of acute pancreatitis was 7.4% (44/595). There was a significant difference in the incidence of PEP with or without nafamostat mesilate (13.0% vs 4.0% and 5.1%, respectively; P < 0.0001). Subgroup analysis showed that in low-risk patients, the rate of PEP was significantly different with nafamostat (11.9% vs 2.7% and 4.0%, respectively; P = 0.007). In high-risk patients, the rate of PEP was not significantly different among treatment groups (14.6% vs 5.9% vs 6.9%, respectively; P = 0.108). CONCLUSIONS:: Nafamostat mesilate prophylaxis (20 or 50 mg) is effective in preventing post-ERCP pancreatitis. However, the preventive effect of high-dose nafamostat mesilate (50 mg) is not significant in high-risk patients.

아몬드 크래커의 과산화 억제에 있어서 천연 항산화제의 활용

박기범(Kee-Bum Park),한규홍(Gyu-Hong Han),김병용(Byung-Yong Kim) 한국식품영양과학회 2003 한국식품영양과학회지 Vol.32 No.1

천연 항산화제인 tocopherol과 rosemary, tea polyphenol을 아몬드 크래커에 첨가하여 25℃와 35℃에서 70일 동안 저장하면서 저장기간에 따른 항산화 효과를 살펴보았다. 과산화물가를 측정하여 품질평가를 한 결과 25℃에서는 rosemary가 가장 우수한 항산화력을 보여주었고, 35℃에서는 tea polyphenol이 우수하였다. 저장하는 동안 아몬드 크래커의 관능 검사에서는 rosemary와 tea polyphenol이 냄새와 맛에 좋은 영향을 주었고, 25℃에서 항산화제를 첨가하지 않은 blank와 tocopherol은 42일, rosemary와 tea polyphenol은 56일이 지난 후에 제품으로서 품질을 잃는 것으로 나타났다. 35℃에서는 25℃에 비하여 모든 기호도 면에서 저하가 빠르게 일어났고, rosemary와 tea polyphenol이 blank와 tocopherol간의 차이가 유의적으로 크게 나타났다. 천연 항산화제의 첨가에 따른 산화정도를 저장기간 동안 과산화물가의 변화를 이용하여 반응속도식를 구하였을 때, 25℃에서는 1차 반응식으로 나타났고, 35℃에서는 0차 반응식이 결정되었다. 이 때 반응속도 상수는 25℃에서 rosemary가 -0.0299, 35℃에서 tea polyphenol이 -0.4038로 가장 낮은 수치를 보여주었다. 그러나 아몬드 크래커를 위한 항산화제 선택에 있어서는 저장온도에 따라 각각의 다른 천연 항산화제가 영향을 주는 만큼 rosemary와 tea polyphenol을 함께 첨가하여 그 synergy 효과를 살피는 연구가 이루어져야 할 것이다. This research was aimed to evaluate the inhibition of oxidative changes of almond crackers by natural antioxidants (tocopherol, rosemary, and tea polyphenol). Almond crackers stored at 25℃ and 35℃ for 70 days were evaluated with peroxide value, sensory and kinetic parameters. Results revealed that the antioxidative activity of rosemary was highly effective in inhibiting lipid oxidation at 25℃ and tea polyphenol highly effective at 35℃. Sensory evaluation indicated that the properties of odor and taste of almond crackers tended to positive effect with the addition of rosemary and tea polyphenol. Kinetic study in lipid oxidation showed the first order reaction at 25℃ and the zero order reaction at 35℃. It was found that the kinetic parameter of oxidation reaction predicted for the shelf-life of almond cracker.

Viral Loads and E2/NS1 Region Sequences of Hepatitis C Virus in Hepatocellular Carcinoma and Surrounding Liver

( Choong Kee Park ),( Tae Rim Park ),( Yong Bum Kim ),( Hak Yang Kim ),( Jae Young Yoo ),( Chun Hyung Kim ),( Sung Ho Choo ),( Joong Myung Cho ) 대한내과학회 1997 The Korean Journal of Internal Medicine Vol.12 No.1

한국의 발톱진균증 환자의 삶의 질에 관한 연구

조백기,박종갑,김형옥,김성욱,백승철,김진우,김시용,안규중,전재복,오지원,김낙인,이규석,오칠환,김수남,김상태,손숙자,신용우,김동석,이원우,권경술,서대헌,황규왕,이종석,고재경,강원형,정기양,최응호,김기홍,박석돈,강승주,함정희,명기범,김방순,구상완,김병수,원영호,김한욱,송은섭,정병수,노병인,홍창권,박장규,한지윤,김광중,구대원,김종민,김재홍,유희준,양경미 대한의진균학회 1998 대한의진균학회지 Vol.3 No.2

Background: Onychomycosis, especially toenail onychomycosis has become one of the common fungal infection and has historically been regarded as a cosmetic rather than medical problem by many patients, even by physicians. Recently, however, there are several reports that is a refractory disease which may cause a deleterious effect on patiets quality of life (QOL). Objective: The purpose of this study was to investigate the impact of toenail onychomycosis on QOL in Korea and to assess the chandes of QOL after treatment. Methods: Total 1004 patients with toenail onychomycosis which was confirmed by clinical findings and KOH preparation were were enrolled at 47 dermatologic centers in Korea, and interviewed with standardized QOL questionnaire before and after sytemic antifungal treatment. Responses to the questionnaire were scored by 5-point scale (1∼4) and averaged, and were analyzed for 5 dimensions of emotional impact, social impact, symptorn and functional impact, patients views concerning treatment, and relationship with doctor. Results: 1. Before and after treatment, the most serious impact was emotional dimension showing 1.90 and 1.30 in average score (AS), and social (AS: 1.14 and 0.83) and symptom and functional impact (AS: 1.05 and 0.92) was also affected. 2. In female rather than male, statistically more significant impact on patients QOL was observed in all dimensions. 3. After treatment, 3 of 5 dimensions were improved significantly - emotional dimension (AS : from 1.90 to 1.30), social dimension (AS: from 1.14 to 0.83), patients view concerning treatment (AS: from 1.34 to 1.02). 4. The drgree of patients satisfaction at the therapeutic effect was very high- 62.4% (immediately after treatment) and 65.8% (9 months affer initiation of treatment) of patients answered excellent or good. Conclusion: This study confirms that toenail onychomycosis has significant impact on the overall QOL of patients. Also the effect of antifungal therapy on patients QOL were watisfactory. Therefore, both doctor and patient should pay more attention to the treatment of onychomycosis. [Kor J Med Mycol 3(1): 115∼124]