韓國のオブバッタおよびショウリョウバッタモドキ集團における染色體變異

茅野 博,吳文儒,茅野愛子 濟州大學校 基礎科學硏究所 1994 基礎科學硏究 Vol.7 No.1

Chromosomes of primary spermatocytes of 17 males of Atractomorpha bedeli collected in a population at Haenam, Chollanam Do, Republic of Korea, were observed. Supernumerary chromosomes (B-chromosomes) were found in five males (2n♂=18₁×10~3 B,Figs.1ㆍ6). The numbers of B's were constant among the cells within the follicles but were variable among the follicles within the testes of the males (Table 1). The characteristics of the B's were the same as those found in the populations in Kyushu, Japan (Sannomiya 1973). The 9th chromosomes (the smallest among the complement) of A. bedeli were of two types, S with a distal supernumerary segment, I. e., segmented, and N, non-segmented (Figs. 1-6, 7-10, 11-16). The supernumerary segment was euchromatic (Figs. 11 and 12). Three kinds of karyomorphs were distinguishable as follows: S/S (a single male), S/N (five males), and N/N (11 males). Chromosomes of primary spermatocytes of 14 males of Gonista bicolor collected in a population in Chejudo, Cheju Do, Republic of Korea, were observed. Seven males were found to have B-chromosomes of two types, B₁and B₂(2n♂=22+X+0~1B₁+0~4B₂;Figs.17-22). The B₁'s were found in two males in which all the primary spermatocytes had a single B₁. The B₂'s were found in five males. The numbers of B₂'s were variable among the follicles of the testes, while the primary spermatocytes within the follicles had the same number of B₂'s (Table 3). The characteristics of B₁and B₂were the same as those found in the populations in Kyushu, Japan (Sannomiya 1974). 1. 韓國全羅南道海南において採集したオンブバッタの雄17個體の第一精母細胞の染色體を觀察した. 12個體では9個の2價染色體と1個の1價染色體(X染色體)が見られたが, 5個體では, 前記の染色體の外にB染色體をもっていた(2n♂=18+X+0~3B). B染色體の數は同一の個體の精巢でも, フォリクルによって異なっていた. しかし. 1つのフォリクルの中では, どの第一精母細胞にも同じ數のB染色體が見られた. 5個體のそれぞれにおけるB染色體の數の變動の範圍は, 0∼2, 0∼2, 1∼2, 0∼3, および, 0∼3であった. これらのB染色體の細胞學的特徵は, Sannomiya (1973) が日本 (九州) の集團について報告したものと同じであった. 2. 韓國海南のオンブバッタ集團では, 常染色體の第9染色體に, 過剩部分をもつS型と, 過剩部分をもたないN型とが區別された. この過剩部分は眞正染色質であった. 二價染色體の特徵から, 接合體の染色體型として, S/S(1個體), S/N(5個體), および, N/N(11個體)の3型が識別された. 3. 韓國濟州島のショウリョウバッタモドキ集團から雄14個體を採集し, 第一精母細胞の染色體を觀察した. 7個體では11個の二價染色體と1個の一價染色體(X染色體)をもっていたが, 他の7個體には前記の染色體の外に, B染色體が含まれていた(2n♂=22+X+0~1B₁+0~4B₂). B₁は2個體に, また, B₂は5個體に見られた. B₁はどの第一精母細胞にも1個見られた. しかし, B₂の數は同一の個體の精巢の中でも, フォリクルによって異なっていた. 5個體のそれぞれにおけるB₂染色體の數の變動の幅は, 1∼2, 1∼2, 1∼2, 1∼3, および, 2∼4であった. B₁およびB₂の特徵は, 日本(九州)のショウリョウバッタモドキの集團について, Sannomiya (1974)が報告したB染色體と同じであった.

韓國のオンブバッタおよびショウリョウバッタモドキ 集團における 染色體變異

茅野愛子,茅野博,吳文儒 濟州大學校 基礎科學硏究所 1993 基礎科學硏究 Vol.6 No.1

Chromosomes of primary spermatocytes of 17 males of Atractomorpha bedeli collected in a population at Haenam, Chollanam Do. Republic of Korea, were observed. Supernumerary chromosomes (B-chromosomes) were found in five males (2n??=18+X+0~3B, figs, 1-6). The numbers of B's were constant among the cells within the follicles but were variable among the follicles within the testes of the males (Table 1). The characteristics of the B's were the same as those found in the populations in Kyushu, Japan (Sannomiya 1973). The 9th chromosomes (the smallest among the complement) of A. bedeli were of two types, S with a distal supernumerary segment, i.e. segmented, and N, non-segmented (Figs, 1-6, 7-10, 11-16). The supernumerary segment was euchromatic (Figs, 11 and 12). Three kinds of karyomorphs were distinguishable as follows: S/S (a single male), S/N (five males), and N/N (1] males). Chromosomes of primary spermatocytes of 14 males of Gonista bicolor collected in a population in Chjudo, Cheju Do, Republic of Korea, were observed. Seven males were found to have B-chromosomes of two type, B₁ and B₂ (2n??=22+X+0~1B₁+0~4B₂; Figs. 17~22). The B₁'s were found in two males in which all the primary spermatocytes had a single B₁. The B₂'s were found in five males. The numbers of B₂'s were variable among the follicles of the testes, while the primary spermatocytes within the follicles had the same number of B₂'s (Table 3). The characteristics of B₁and B₂were the same as those found in the populations in Kyushu, Japan (Sannomiya 1974). 1.韓國全羅南道海南において授集したオンブバッタの雄17個體の第一精母細胞の染色體を觀察した. 12個體では9個の2價染色體と1個の1價染色體(X染色體)が見られたが, 5個體では, 前記の染色體の外にB染色體をもっていた(2n?=18+X+0~3B). B染色體の數は同一の個體の精巢でも, フォリクルによって異なっていた. しかし. 1つのフォリクルの中では, どの第一精母細胞にも同じ數のB染色體が見られた. 5個體のそれぞれにおけるB染色體の數の變動の範圍は, 0~2, 0~2, 1~2, 0~3, および, 0~3であった. これらのB染色體の細胞學的特徵は, Sannomiya(1973)が日本(九州)の集團について報告したものと同じであった. 2.韓國海南のオンブバッタ集團では, 常染色體の第9染色體に, 過剩部分をもつS型と, 過剩部分をもたないN型とが區別された. この過剩部分は眞正染色質であった. 二價染色體の特徵から, 接合體の染色體型として, S/S(1個體), S/N(5個體), および, N/N(11個體)の3型が識別された. 3.韓國濟州島のショウリョウバッタモドキ集團から雄14個體を採集し, 第一精母細胞の染色體を견察した. 7個體では11個の二價染色體と1個の一價染色體(X染色體)をもっていたが, 他の7個體には前記の染色體の外に, B染色體が含まれていた(2n=22+X+0~1 B₁+0~4 B₂). B₁は2個體に, また, B₂は5個體に見られた. B₁はどの第一精母細胞にも1個見られた. しかし, B₂の數は同一の個體の精巢の中でも, フォリクルによって異なっていた. 5個體のそれぞれにおけるB₂染色體の數の變動の幅は, 1~2, 1~2, 1~2, 1~3, および, 2~4であった. B₁およびB₂の特徵は, 日本(九州)のショウリョウバッタモドキの集團について, Sannomiya(1974)が報告したB染色體と同じであった.

Current Consensus on I-131 MIBG Therapy

Daiki Kayano,Seigo Kinuya 대한핵의학회 2018 핵의학 분자영상 Vol.52 No.4

Metaiodobenzylguanidine (MIBG) is structurally similar to the neurotransmitter norepinephrine and specifically targets neuroendocrinecells including some neuroendocrine tumors. Iodine-131 (I-131)-labeled MIBG (I-131 MIBG) therapy for neuroendocrinetumors has been performed for more than a quarter-century. The indications of I-131 MIBG therapy include treatmentresistantneuroblastoma (NB), unresectable or metastatic pheochromocytoma (PC) and paraganglioma (PG), unresectable ormetastatic carcinoid tumors, and unresectable or metastatic medullary thyroid cancer (MTC). I-131 MIBG therapy is one of theconsiderable effective treatments in patients with advanced NB, PC, and PG. On the other hand, I-131 MIBG therapy is analternative method after more effective novel therapies are used such as radiolabeled somatostatin analogs and tyrosine kinaseinhibitors in patients with advanced carcinoid tumors and MTC. No-carrier-aided (NCA) I-131 MIBG has more favorablepotential compared to the conventional I-131 MIBG. Astatine-211-labeled meta-astatobenzylguanidine (At-211 MABG) hasmassive potential in patients with neuroendocrine tumors. Further studies about the therapeutic protocols of I-131 MIBGincluding NCA I-131 MIBG in the clinical setting and At-211 MABG in both the preclinical and clinical settings are needed.

Al-4 % Cu 합금의 석출에 미치는 중성자조사의 효과

송진태,모야수부 대한금속재료학회(대한금속학회) 1974 대한금속·재료학회지 Vol.12 No.1

Al-4%Cu 合金의 析出過程에 미치는 高速性中子의 照射效果를 Vickers 硬度의 測定 및 透過電子顯微鏡으로 관측 고찰하였다. 中性子照射는 日本原子力硏究所材料試驗爐(JMTR)內에서 行하였고 照財中性子量은 各各 2.8×10^(19), 3.4×10^(20), 1.2×10^(21)nvt였다. θ'折出粒子의 成長速度의 觀測은 電子顯微鏡의 hot stage를 사용하였고 測定資料는 析出物의 成長機構에 관한 最近의 ledge 理論式들에 적용 검토하였다. 그 結果 照射한 試料의 擴散係數 및 Arrhenius 振動數項은 未照射試料보다 훨씬 컸고 이것은 照射에 依해서 生成된 過剩空孔(excess vacancies) 또는 interstitials에 기인하는 것이라 믿어진다. 實驗結果는 中性子의 照射가 不均一核生成을 증가시킬 뿐만 아니라 溶質原子의 擴散을 促進시키며 이러한 效果는 照財線量이 많을수록 크며 析出物 成長速度는 보다 커진다는 것을 보여주고 있다. In order to examine the effects of fast neutron irradiation on the precipitation process in Al-4%Cu alloy, hardness measurement and transmission electron microscope were employed. Specimens were irradiated to 2.8×10^(19), 3.4×10^(20) and 1.2×10^(21) nvt neutron doses in the Japan Materials Testing Reactor. Their electron micrographs prior to annealing or at the early stage of annealing at 200℃ showed G. P. 2 and even θ' plates, which it can not normally expect to form. The kinetics of growing of θ'plates in Al-4%Cu alloy were measured by means of hot stage transmission electron microscopy, and the lengthening data of θ'plate measured at each temperature and at each neutron dose have been interpreted in terms of the various equations for recent ledge theories of growth. The calculated diffusivity and frequency factor of Arrhenius' equation of specimen irradiated to 2.3×10^(19) nvt neutron are much larger than the un-irradiated one. This may be attributed to excess vacancies or interstitials produced by irradiation. It is concluded that neutron irradiation does not only bring the enhancement of heterogeneous nucleation, but also give rise to enhanced diffusion of solute atom, and the more the neutron dose increases, the greater the growth rate becomes.

Restriction Enzyme Analysis of Mitochondrial DNA in Cheju and Tsushima Native Horses and Evolutionary Relationships

Oh,Y.S.,Kayano, Hiroshi,Jung,Y.H.,Oh,M.Y.,Hong,S.S.,Kim,S.J.,Kim,G.O.,Ko,M.H. 濟州大學校 基礎科學硏究所 1997 基礎科學硏究 Vol.10 No.2

네 개의 다른 말 집단(Cheju native horses I; Cheju Racing horses, Cheju native horses II; Chejudo Livestock Promotion Institute horses, Tsushima native horses, Thoroughbreds)에서 mitochondrial DNA(mtDNA)의 제한효소 단편양상을 연구하였다. 122개체의 total DNA는 말의 mtDNA probe를 사용하여 18개의 제한효소 (Bam HⅠ, EcoRⅠ, HindIll, A vall,HpaⅠ,BglⅡ, XbaⅠ,PstⅠ,pvuⅡ,SacⅠ, KpnI, XhoI, AvaI, HincII, Bst EII, NotI, AccI, and HaeII)에 대한 분석을 하였다. 네 집단에서 16개의 다른 단편양상(mtDNA types)을 조합한 결과 12개의 Morphs를 관찰하였다. 네 집단에서 12개의 restriction types를 갖고 UPGMA 방법에 의하여 계통수를 작성한 결과 2개의 clusters로 분산되었다. 첫 번째 cluster는 주로 1, 6, 3, 8, 9, 2 types, 두 번째 cluster는 4, 7, 10, 11, 12 types들로 각각 구성되었다. 쓰시마재래마는 mtDNA types의 동질성이 높은 빈도를 보였다. 제한 효소 단편양상에서 제주재래마 두 집단과 Thoroughbreds 집단은 polymorphic한 반면, 쓰시마재래마는 monomorphic(1-2-1-1 mtDNA type)함을 보였다. 제주재래마집단 II와 쓰시마재래마가 진화학적 유연관계가 가장 가깝게 나타났으며, 두 집단간에 유전적거리는 1.5×10-3으로 추정되었다. Mitochondrial DNA(mtDNA) restriction endonuclease fragment patterns were studied in four different equine populations(Cheju native horses I; Cheju Racing horses, Cheju native horses II; Chejudo Livestock Promotion Institute horses, Tsushima native horses, Thoroughbreds). Total DNA samples from 122 individuals were analysed with 18 restriction enzymes, Bam HI, EcoRI, HindlⅡ,AvaⅡ,HpaⅠ, BglⅡ,XbaⅠ,PstⅠ,PvuⅡ,SacⅠ, KpnⅠ, XhoI, AvaI, HincII, BstEII, NotI, AccI, and HaeII using the horse mtDNA probe. Twelve morphs were observed yielding sixteen different combinations of fragment patterns(mtDNA types) in four populations. The phylogenetic tree constructed from the 12 restriction types by the UPGMA method indicated that mtDNA types in four populations were divided into two clusters. In this tree, the clear separation into the two clusters was observed, namely one with types 1, 6, 3, 8, 9, 2, and 5, and the other one with types 4, 7, 10, 11, and 12. Tsushima native horses were found to be highly homogeneous by the frequencies of mtDNA types. The populations of Cheju native horses I, II, and Thoroughbreds showed polymorphism, while the population of Tsushima native horses was monomorpic(only 1-2-1-1 type), acording to the restriction enzymes. Cheju native horses II were shown to be closely related to the Tsushima native horses, and the genetic distance between these two populations was 1.5×10-3.

Polymorphism of the MtDNA Cytochrome B and NADH Dehydrogenase 6 Genes in Tsushima and Juju Native Horses

한상현,김재환,송지훈,오주형,오유성,정용환,Hiroshi Kayano,오문유 한국유전학회 2004 Genes & Genomics Vol.26 No.1

Polymorphism of the MtDNA Cytochrome B and NADH Dehydrogenase 6 Genes in Tsushima and Jeju Native Horses

Han, Sang-Hyun,Kim, Jae-Hwan,Song, Ji-Hoon,Oh, Ju-Hyung,Oh, You-Sung,Jung, Yong-Hwan,Kayano, Hiroshi,Oh, Moon-You 한국유전학회 2004 Genes & Genomics Vol.26 No.1

We analyzed the mitochondrial DNA (mt DNA) of horses using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and DNA sequencing. The mtDNA fragment containing the NADH dehydrogenase subunit 6 (ND6), cytochrome B (cytB), tRNA-Glu, and tRNA-Thr genes, was analyzed. We boserved polymorphic patterns using four restriction enzymes: BamHI, Hinf1, Msp1, and Rsa1. Nine mtDNA haplotypes were found in Jeju native horses, while no haplotype was specific for thoroughbreds of Tsushima native horses. The mtDNA of Jeju native horses, which is maternally inherited, was found to be highly polymorphic. This result was supproted by previous reports on the genetic diversity of extant Juju horses. Recent archaeological reports confirmed that horses other than those extant on Jeju were present before the Mongol invasion. We suggest that horse were present of Jeju Island in ancient times, and then Mongolian and other lorses were introduced to the island, where they had the opportunity to interbreed, resulting in the present high level of mtDNA polymorphism. Moreover, our results should prove useful for identifying maternity and determining the evolutionary relationships among horse populations.