http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Karwasara, Vijai Singh (검색결과 3 건)
- 무료
- 기관 내 무료
- 유료
-
A simple, rapid and sensitive spectrofluorimetric method for the determination of camptothecin
Karwasara, Vijai Singh,Nahata, Alok,Dixit, Vinod Kumar 경희한의학연구센터 2012 Oriental Pharmacy and Experimental Medicine Vol.12 No.2
Camptothecin (CPT) represents a clinically useful class of anticancer agent. Proper identification and quantitation of the CPT in the plant extracts and in-vitro cell culture extracts is fundamental to assess the CPT content and its biosynthetic potential in plants. A simple, sensitive and rapid, spectrofluorimetric method has been developed and validated for the quantitative estimation of camptothecin. The method was validated in terms of linearity (2-20 ng/ml), precision (intra-day variation below 0.15, interday variation below 1.2), and accuracy (98.0 to 100.2%). The limit of detection and limit of quantification for CPT were found to be 0.10 ng/ml and 0.36 ng/ml, respectively. The developed spectrofluorimetric method provides a rapid and cost effective method for the routine analysis of CPT in plant extracts and tissue culture samples. The developed method was successfully used for the estimation of CPT in natural plant extracts and cell culture extracts. The Nothapodytes nimmoniana callus cells having nearly 3-fold higher CPT content over the leaf (0.005%) explant of the plant. The highest CPT content was found in the stem part (0.092%) followed by the fruit (0.088%). The method is simple, sensitive and precise; it can be used for the routine quality control testing of formulations containing CPT.
-
Karwasara, Vijai Singh,Dixit, Vinod Kumar 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.3
The effect of culture medium nutrients on growth and alkaloid production by plant cell cultures of Nothapodytes nimmoniana (J. Grah.) Mabberley (Icacinaceae) was studied with a view to increasing the production of the alkaloid camptothecin, a key therapeutic drug used for its anticancer properties. Amongst the various sugars tested with Murashige and Skoog (MS) medium, such as glucose, fructose, maltose, and sucrose, maximum accumulation of camptothecin was observed with sucrose. High nitrate in the media supports the biomass, while high ammonium enhances the camptothecin content. Selective feeding of 60 mM total nitrogen with a $NH_4{^+}/NO_3{^-}$ balance of 5/1 on day 15 of the culture cycle results in a 2.4-fold enhancement in the camptothecin content over the control culture ($28.5{\mu}g/g$ DW). Furthermore, the sucrose feeding strategy greatly stimulated cell biomass and camptothecin production. A modified MS medium was developed in the present study, which contained 0.5 mM phosphate, a nitrogen source feeding ratio of 50/10 mM $NH_4{^+}/NO_3{^-}$ and 3 % sucrose with additional 2 % sucrose feeding (added on day 12 of the cell culture cycle) with $10.74{\mu}M$ naphthaleneacetic acid and $0.93{\mu}M$ kinetin. Finally, the selective medium has 1.7- and 2.3-fold higher intracellular and extracellular camptothecin content over the control culture (29.2 and $8.2{\mu}g/g$ DW), respectively.
-
Vijai Singh Karwasara,Vinod Kumar Dixit 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.3
The effect of culture medium nutrients ongrowth and alkaloid production by plant cell cultures ofNothapodytes nimmoniana (J. Grah.) Mabberley (Icacinaceae)was studied with a view to increasing the productionof the alkaloid camptothecin, a key therapeutic drug usedfor its anticancer properties. Amongst the various sugarstested with Murashige and Skoog (MS) medium, such asglucose, fructose, maltose, and sucrose, maximum accumulationof camptothecin was observed with sucrose. Highnitrate in the media supports the biomass, while highammonium enhances the camptothecin content. Selectivefeeding of 60 mM total nitrogen with a NH4?/NO3- balanceof 5/1 on day 15 of the culture cycle results in a 2.4-fold enhancement in the camptothecin content over thecontrol culture (28.5 lg/g DW). Furthermore, the sucrosefeeding strategy greatly stimulated cell biomass and camptothecinproduction. A modified MS medium was developedin the present study, which contained 0.5 mMphosphate, a nitrogen source feeding ratio of 50/10 mMNH4?/NO3- and 3 % sucrose with additional 2 % sucrosefeeding (added on day 12 of the cell culture cycle) with10.74 lM naphthaleneacetic acid and 0.93 lM kinetin. Finally, the selective medium has 1.7- and 2.3-fold higherintracellular and extracellular camptothecin content overthe control culture (29.2 and 8.2 lg/g DW), respectively.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
