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Lee, Ok-Sun,Kang, Young-Min,Jung, Hee-Young,Min, Ji-Yun,Kang, Seung-Mi,Chandrakant S. Karigar,D. Theertha Prasad,Bahk, Jung-Dong,Choi, Myung-Suk Plant molecular biology and biotechnology research 2005 Plant molecular biology and biotechnology research Vol.2005 No.
In wild-type Scopolia parviflora (Solanaceae) tissues, only the roots express the enzyme putrescine N-methyltransferase (PMT; EC 2.1.1.53), which is the first specific precursor of the tropane alkaloids. Moreover, the tropane alkaloid levels were the highest in the root (0.9mg g^(-1) on a dry weight basis), followed by the stem and then the leaves. We metabolically engineered S. parviflora by introducing the tobacco pmt into its genome by a binary vector system that employs disarmed Agrobacterium rhizogenes. The kanamycin-resistant hairy root lines were shown to bear the pmt gene and to overexpress its mRNA and protein product by at least two-fold, as determined polymerase chain reaction (PCR) and Northern and Western blottings, respectively. The transgenic lines also showed higher PMT activity and were morphologically aberrant in terms of slower growth and the production of lateral roots. The overexpression of pmt markedly elevated the scopolamine and hyoscyamine levels in the transgenic lines that showed the highest pmt mRNA and PMT protein levels. Thus, overexpression of the upstream regulator of the tropane alkaloid pathway enhanced the biosynthesis of the final product. These observations may be useful in establishing root culture systems that generate large yields of tropane alkaloids.
Enzymatic Saccharification of Salix viminalis cv. Q683 Biomass for Bioethanol Production
Kim, Hak-Gon,Song, Hyun-Jin,Jeong, Mi-Jin,Sim, Seon-Jeong,Park, Dong-Jin,Yang, Jae-Kyung,Yoo, Seok-Bong,Yeo, Jin-Ki,Karigar, Chandrakant S.,Choi, Myung-Suk Institute of Forest Science 2011 Journal of Forest Science Vol.27 No.3
The possibility of employing biomass of Salix viminalis cv. Q683 as a resource of bio-energy was evaluated. The chemical analysis of S. viminalis cv. Q683 leaf biomass showed components such as, extractives (2.57%), lignin (39.06%), hemicellulose (21.61%), and cellulose (37.83%), whereas, its stem was composed of extractives (1.67%), lignin (23.54%), hemicellulose (33.64%), and cellulose (42.03%). The biomass of S. viminalis cv. Q683 was saccharified using two enzymes celluclast and viscozyme. The saccharification of S. viminalis cv. Q683 biomass was influenced by enzymes and their strengths. The optimal enzyme combination was found to be celluclast (59 FPU/g substrate) and viscozyme (24 FBG/g substrate). On saccharification the glucose from leaf and stem biomass was 7.5g/L and 11.7g/L, respectively after 72 hr of enzyme treatment. The biomass and enzyme-treated biomass served as the feedstock for ethanol production by fermentation. The ethanol production from stem and leaf biomass was 5.8 g/L and 2.2 g/L respectively, while the fermentation of the enzymatic hydrolysates yielded 5 g/L to 8 g/L bioethanol in 72 hours.
Enzymatic Saccharification of Salix viminalis cv. Q683 Biomass for Bioethanol Production
Hak-Gon Kim,Hyun-Jin Song,Mi-Jin Jeong,Seon-Jeong Sim,Dong-Jin Park,Jae-Kyung Yang,Seok-Bong Yoo,Jin-Ki Yeo,Chandrakant S. Karigar,Myung-Suk Choi 강원대학교 산림과학연구소 2011 Journal of Forest Science Vol.27 No.3
The possibility of employing biomass of Salix viminalis cv. Q683 as a resource of bio-energy was evaluated. The chemical analysis of S. viminalis cv. Q683 leaf biomass showed components such as, extractives (2.57%), lignin (39.06%), hemicellulose (21.61%), and cellulose (37.83%), whereas, its stem was composed of extractives (1.67%), lignin (23.54%), hemicellulose (33.64%), and cellulose (42.03%). The biomass of S. viminalis cv. Q683 was saccharified using two enzymes celluclast and viscozyme. The saccharification of S. viminalis cv. Q683 biomass was influenced by enzymes and their strengths. The optimal enzyme combination was found to be celluclast (59 FPU/g substrate) and viscozyme (24 FBG/g substrate). On saccharification the glucose from leaf and stem biomass was 7.5g/L and 11.7g/L, respectively after 72 hr of enzyme treatment. The biomass and enzyme-treated biomass served as the feedstock for ethanol production by fermentation. The ethanol production from stem and leaf biomass was 5.8 g/L and 2.2 g/L respectively, while the fermentation of the enzymatic hydrolysates yielded 5 g/L to 8 g/L bioethanol in 72 hours.
김용덕,윤재길,Chandrakant S. Karigar,최명석 한국원예학회 2015 원예과학기술지 Vol.33 No.1
Effects of mineral salts (N, P, K, Ca2+, Mg2+, and Fe3+) on the shoot growth and metabolite production of tea tree were studied u sing in vitro culture techniques. Among mineral s alts, H2PO4- was the most important for enhanced growth rate of tea tree, while Mg2+ and Ca2+ did not affect plant growth. Removal of NH4+ and NO3 from the culture medium enhanced shoot multiplication compared to other treatments. Metabolite production was variable depending on mineral types and concentration. Removal of Ca2+ decreased the production of caffeine; however, other treatments did not influence its production. Ca2+, NH4+ and Fe3+ were important factors for catechin production in tea tree. These results can be used as the basis for development of technical soil controls suitable for tea tree cultivation in the future.
Efficient Release of Ferulic Acid from Sweet Potato (Ipomoea batatas) Stems by Chemical Hydrolysis
최명석,이종윤,이병현,정강원,Chandrakant S. Karigar,Ji-Hyun Park,Ji Yun Min,Bu-Kug Lim,양재경 한국생물공학회 2008 Biotechnology and Bioprocess Engineering Vol.13 No.3
An efficient method for release of ferulic acid from sweet potato stems was developed. Ferulic acid along with phenolic compounds were released from stems by acid and alkaline treatments. The base hydrolysis with 0.1 N NaOH yielded the highest quantity of total extracts (471.1 mg/g). The stems released more phenolic compounds when 0.0125~0.025 N NaOH was employed. Where as ferulic acid release was maximal with 0.05 N H₂SO₄ (0.32 mg/g). Ferulic acid was separated from phenolics by column chromatography. Among the elution solvents, ethyl acetate fractions (80%) contained ferulic acid. Ethyl acetate eluants were further fractionated with n-hexane/ethyl acetate/formic acid (100/50/0.5, v/v/v). All fractions showed ferulic acid and phenolic compounds. Fraction V among them was ascribed to ferulic acid with an yield of 5.41 mg/g of dry sweet potato tissue.
강승미,민지연,박동진,송현진,허창미,문현식,김종갑,Chandrakant S. Karigar,최명석,정미진 경상대학교 농업생명과학연구원 2011 농업생명과학연구 Vol.45 No.1
An efficient method for the rapid micropropagation of Camptotheca acuminata from axillary buds was established by application of various plant growth regulators. Among various cytokinins, 0.5 mg L^(-1) BA showed the best performance on shoot multiplication, number average multiple shoots up to 10.8. The propagated shoot cuttings in vitro were elongated on NN basal medium without plant growth regulators. The secondary multiple shoots were induced at the site of initially induced buds. Rooting was induced directly near the base of the shoot on half-strength NN medium containing 0.5 mg L^(-1) of IBA, whereas high concentration of 1.0 mg L^(-1) IBA could induce callus at the base of the shoot. The camptothecin content, anticancer compound of the micropropagated plants was contained in various tissues. Camptothecin contents were 1.8 and 2.5 mg g^(-1) dry weight in stems from propagated in vitro and mother plant, respectively. This result may be used to develop strategies for large-scale propagation of elite C. acuminata trees.
In vitro selection of salt-tolerant Ailanthus altissima Swingle
강영민,송현진,문현식,이철호,김종갑,Chandrakant S. Karigar,최명석 한국산림과학회 2012 Forest Science And Technology Vol.8 No.1
Salt-tolerant cell lines of Ailanthus altissima were selected from callus derived protoplasts. Murashige–Skoog (MS) liquid medium incorporated with various concentrations of NaCl was employed to enrich salt-tolerant A. altissima cell lines. Salt-resistant A. altissima cells were transferred on MS solid medium supplemented with 2.5 μM 2,4- dichlorophenoxy acetic acid (2,4-D), 0.5 μM benzyl adenine (BA) and various NaCl concentrations. The callus was cultured on MS medium containing NaCl for 5 months, to determine the survival rate as an index of salt tolerance. The measurement of growth parameters for salt-tolerant cells showed that the selected plant cell lines grew better than the unselected ones at all levels of NaCl tested. The salt-tolerant callus accumulated proline in correlation to the concentration of salts. Media supplemented with BA induced shoot differentiation of salt-resistant A. altissima cells.
Kim, Yong Duck,Yun, Jae Gill,Seo, Yeong Rong,Karigar, Chandrakant S.,Choi, Myung Suk Korean Society of Horticultural Science 2015 원예과학기술지 Vol.33 No.1
Effects of mineral salts (N, P, K, $Ca^{2+}$, $Mg^{2+}$, and $Fe^{3+}$) on the shoot growth and metabolite production of tea tree were studied using in vitro culture techniques. Among mineral s alts, ${H_2PO_4}^-$ was the most important for enhanced growth rate of tea tree, while $Mg^{2+}$ and $Ca^{2+}$ did not affect plant growth. Removal of ${NH_4}^+$ and $NO_3$ from the culture medium enhanced shoot multiplication compared to other treatments. Metabolite production was variable depending on mineral types and concentration. Removal of $Ca^{2+}$ decreased the production of caffeine; however, other treatments did not influence its production. $Ca^{2+}$, ${NH_4}^+$ and $Fe^{3+}$ were important factors for catechin production in tea tree. These results can be used as the basis for development of technical soil controls suitable for tea tree cultivation in the future.
Yong Duck Kim,Jae Gill Yun,Yeong Rong Seo,Chandrakant S. Karigar,Myung Suk Choi 한국원예학회 2015 원예과학기술지 Vol.33 No.1
Effects of mineral salts (N, P, K, Ca<SUP>2+</SUP>, Mg<SUP>2+</SUP>, and Fe<SUP>3+</SUP>) on the shoot growth and metabolite production of tea tree were s tudied u sing in v itro culture techniques. Among mineral s alts, H₂PO₄? was the most important for enhanced growth rate of tea tree, while Mg<SUP>2+</SUP> and Ca<SUP>2+</SUP> did not a ffect plant growth. Remov al o f NH₄? and NO₃ from the culture medium enhanced shoot multiplication compared to other treatments. Metabolite production was variable depending on mineral types and concentration. Removal of Ca<SUP>2+</SUP> decreased the production of caffeine; however, other treatments did not influence its production. Ca<SUP>2+</SUP>, NH₄? and Fe<SUP>3+</SUP> were important factors for catechin production in tea tree. These results can be used as the basis for development of technical soil controls suitable for tea tree cultivation in the future.
Rapid Micropropagation of Hovenia dulcis Thunb. Through in vitro Stem Nodal Cultures
Park, Dong-Jin,Kang, Young-Min,Jung, Ha-Na,Min, Ji-Yun,Kim, Yong-Duck,Karigar, Chandrakant S.,Choi, Myung-Suk Korean Society of Forest Science 2006 한국산림과학회지 Vol.95 No.2
An efficient method for in vitro propagation of the medicinal plant Hovenia duleis, was established. Plantlets for micropropagation of H. dulcis were obtained from in vitro germinated seeds. The effectiveness of various levels of cytokinins (BAP, Kinetin and TDZ) on multiple shoot formation from stem nodes was tested. BAP (1.0 mg/L) treatment induced highest number of multiple shoots. The growth pattern of plantlet on various culture media was undertaken. The shoot elongation was optimal on 2MS basal medium without growth regulators. The in vitro rooting ability of H. dulcis shoots was examined with two-auxins IAA and IBA. The IAA (1.0 mg/L) treatments induced earliest rooting with maximum number of roots and root growth. Rooted shoots were transferred directly to small pots with artificial soil and such established plant exhibited a normal growth pattern similar to wild plantlet.