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Release of Renal Dipeptidase from Rabbit Renal Proximal Tubules and Its Inhibition by Gentamicin
Kang, Bok-Yun,We, Jeoung-Soon,Choi, Kyong,Lee, Hwanghee-Blaise,Han, Ho-Jae,Park, Haeng-Soon The Pharmaceutical Society of Korea 1999 Archives of Pharmacal Research Vol.22 No.4
Effects of several durgs on rabbit renal proximal tubules were examined for the applicability of renal dipeptidase (RDPase, EC 3. 4. 13. 11) release as a model system to study nephrotoxicity. The proximal tubule prepared by the method of Taub (1990) released RDPase spontaneously in the control experiment which was confirmed by Western blotting. RDPase was also released form cisplatin, lipopolysaccardie (LPS), and indomethacin-treated tubules. Gentamicin inhibited RDPase release in a concentration-dependent manner. This RDPase release system may not be a general model to screen nephrotoxicity but could be a useful source of RDPase purification in a simple and inexpensive way.
Release of Renal Dipeptidase from Rabbit Renal Proximal Tubules and Its Inhibition by Centamicin
Kang, Bok Yun,We, Jeoung Soon,Choi, Kyong,Lee, Hwanghee blaise,Han, Ho Jae,Park, Haeng Soon 전남대학교 약품개발연구소 1999 약품개발연구지 Vol.8 No.1
Effects of several drugs on rabbit renal proximal tubules were examined for the applicability of renal dipeptidase (RDPase, EC 3. 4. 13. 111 release as a model system to study nephrotoxicity. The proximal tubule prepared by the method of Taub (1990) released RDPase spontaneously in the control experiment which was confirmed by Western blotting. RDPase was also released from cisplatin, lipopolysaccharide (LPS), and indomethacin-treated tubules. Gentamicin inhibited RDPase release in a concentration-dependent manner. This RDPase release system may not be a general model to screen nephrotoxicity but could be a useful source of RDPase purification in a simple and inexpensive way.
Release of Renal Dipeptidase from Rabbit Renal Proximal Tubules and Its Inhibition by Gentamicin
Kang, Bok Yun,We, Jeoung Soon,Choi, Kyong,Lee, Hwanghee blaise,Han, Ho Jae,Park, Haeng Soon 전남대학교 약품개발연구소 2000 약품개발연구지 Vol.9 No.1
Effects of several drugs on rabbit renal proximal tubules were examined for the applicability of renal dipeptidase (RDPase, EC 3. 4. 13. 11) release as a model system to study nephrotoxicity. The proximal tubule prepared by the method of Taub (1990) released RDPase spontaneously in the control experiment which was confirmed by Westem blotting. RDPase was also released from cisplatin, lipopolysaccharide (LPS), and indomethacin-treated tubules. Gentamicin inhibited RDPase release in a concentration-dependent manner. This RDPase release system may not be a general model to screen nephrotoxicity but could be a useful source of RDPase purification in a simple and inexpensive way.
인삼이 적혈구세포의 해당과정 및 막 투과도에 미치는 영향
강복순(Kang, Bok-Soon),한경희(Han, Kyung-Hee) 대한생리학회 1983 대한생리학회지 Vol.17 No.2
Red cell glycolytic intermediates, metabolites and metabolic ratios were studied. Glycolytic intermediates were measured in neutralized perchloric acid extracts of red cell suspensions after 3 hr incubation at 37℃ in the presence and absence of saponin. Adenosine triphosphate(ATP), adenosine diphosphate(ADP), pyruvate and lactate were measured by enzymatic procedures involving stoichiometric oxidation or reduction of a pyridine nucleotide. Glucose was determined using glucose oxidase after zinc hydroxide extraction. The redox state was calculated from the lactate dehydrogenase equilibrium. Adenosine triphosphatase activity(ATPase) was measured by determining the amount of phosphate released from ATP by washed erythrocyte membranes(ghost) during 20 min. incubation. Both total hydrolysis and the amount of hydrolysis that occured in the presence of ouabain were measured. The second measurement yields Mg-ATPase and represents nonspecific ATPase activity of the membranes. The difference between total and Mg-ATPase activity can be attributed to Na-K-ATPase. For the measurement of sodium fluxes, human erythrocytes were preincubated in <sup>22</sup>Na for 3 hr at 37℃, washed and suspended in a tracer-free medium. The amount of <sup>22</sup>Na transported out of cells at any time was determined by analysis of supernatant samples taken at various time after addition of the labeled cells to isotope-free medium. The cells and medium were separated and the radioactivity appearing in the medium was measured. From the total radioactivity in the suspension and the radioactivity appearing in the medium at known time, the rate constant for sodium release was computed. The results are summarized as follows: 1) ATP and ATP/ADP were found to increase at every concentration of saponin tested whereas ADP declined at every cone. of saponin. The increase in pyruvate and lactate were observed at every cone, of saponin and thus NAD<sup>+</sup>/NADH computed from pyruvate/lactate also increased. Glucose utilization was stimulated by saponin. 2) Na<sup>+</sup>-K<sup>+</sup>-ATPase activities showed a biphasic response to saponin, first increasing in lower concentration and then decreasing in higher concentration of saponin. 3) The efflux of sodium was significantly increased by saponin in the range of 5 to 10 mg%. The stimulatory effect of saponin on the rate constants for active(ouabain-sensitive) sodium efflux was inhibited by addition of ouabain.
Dae-Weon Lee,Jong Bok Seo,Jae Soon Kang,A-YoungKim,Won Tae Kim,Yeon Ho Je,Kook Jin Lim,Si Hyeock Lee,Young Ho Koh 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.10
There are increasing interests in developing methods specifically detecting pathogenic Bursaphelenchus xylophilus. In order to develop a detecting method for B .xylophilus, at first we generated monoclonal antibodies (MAbs) specific to B. xylophilus, discriminating from other pine tree resident nematodes. Among 2304 hybridoma fusions screened. We finally selected a MAb clone, 9F10 and used for further study. To identify the antigenic target of MAb-9F10, we employed several biochemical methods such as SDS-PAGE, 2 dimensional electrophoresis, anion exchange chromatography, and immunoprecipitation to separate and isolate an antigenic target. Proteins from above methods were analyzed via nano-LC-ESI-Q-IT-MS. Peptides of GaLECtin were always detected from several proteomic analyses, suggesting that GaLECtin is the antigenic target of MAb-9F10.
범가자미 , Verasper variegatus 의 연령과 성장
강용주,박병하,전복순,전임기 한국어류학회 1996 韓國魚類學會誌 Vol.8 No.1
1992년 12월부터 1993년 12월까지 매월 1회씩 남해안에서 어획된 범가자미를 대상으로 연령과 성장을 조사하였다. 연령사정은 이식으로 하였으며, 윤문은 투명대에서 불투명대로 이행하는 경계로 하였다. 윤문은 2월부터 3월 사이에 연 1회 형성되었다. 이석경(R)에 대한 전장(TL)의 관계를 보면 암컷은 TL=18.0329R- 11.6879, 수컷은 TL=15.1458R-5.6902였다. 전장(TL)에 대한 체중(BW)의 관계는 암컷은 BW=0.0065TL^(3.2042), 수컷은 BW=0.0146TL^(2.9620)이었다. 연령(t)에 대한 체장(Lt)의 Von Bertalanf 성장식은 암컷은 L_t= 71.0783(1-e^(-0.3038(t-0.1173))), 수컷은 L_t=57.4414(1-e^(0.2981(t+0.1590)))이었다. 채집된 표본 중 암컷은 6세, 수컷은 4세까지 있었지만 대부분 3세 이하였다. Age and growth of spotted halibut, Verasper variegatus were determined using samples collected from commercial catch in the Southern Sea, Korea. Age was determined by counting the otolith annuli which were clearly defined between translucent and opaque zones, and formed between February and March. Growth of spotted halibut was expressed by the von Bertalanffy's equation as L_t= 71.07(1-e^(-0.3036(t-0.1173))) for females and L_t=57.44(1-e^(0.2981(t+0.1590))) for males. Almost all the fish examined were younger than 3 years old. The oldest fish was 6 years old in female and 4 years old in male.