VP2 capsid domain of the H-1 parvovirus determines susceptibility of human cancer cells to H-1 viral infection

Cho, I-R,Kaowinn, S,Song, J,Kim, S,Koh, S S,Kang, H-Y,Ha, N-C,Lee, K H,Jun, H-S,Chung, Y-H Nature America, Inc. 2015 Cancer gene therapy Vol.22 No.5

Although H-1 parvovirus is used as an antitumor agent, not much is known about the relationship between its specific tropism and oncolytic activity. We hypothesize that VP2, a major capsid protein of H-1 virus, determines H-1-specific tropism. To assess this, we constructed chimeric H-1 viruses expressing Kilham rat virus (KRV) capsid proteins, in their complete or partial forms. Chimeric H-1 viruses (CH1, CH2 and CH3) containing the whole KRV VP2 domain could not induce cytolysis in HeLa, A549 and Panc-1 cells. However, the other chimeric H-1 viruses (CH4 and CH5) expressing a partial KRV VP2 domain induced cytolysis. Additionally, the significant cytopathic effect caused by CH4 and CH5 infection in HeLa cells resulted from preferential viral amplification via DNA replication, RNA transcription and protein synthesis. Modeling of VP2 capsid protein showed that two variable regions (VRs) (VR0 and VR2) of H-1 VP2 protein protrude outward, because of the insertion of extra amino-acid residues, as compared with those of KRV VP2 protein. This might explain the precedence of H-1 VP2 protein over KRV in determining oncolytic activity in human cancer cells. Taking these results together, we propose that the VP2 protein of oncolytic H-1 parvovirus determines its specific tropism in human cancer cells.

Relationship between Stress Gene Polymorphisms and Litter Size by AI in Pigs

H. J. Jin,I. C. Kim,M. S. Wee,S. H. Yeon,C. D. Kim,S. S. Lee,C. Y. Cho,S. R. Cho,D. S. Son,C. K. Park,Z. D. Li 사단법인 한국동물생명공학회 2007 한국동물생명공학회지 Vol.22 No.4

This study was performed to investigate the relationship between PSS-HSP70 gene polymorphism and artificial insemination (AI) reproductivity in the pigs. The RFLP polymorphism of PSS and the SSCP polymorphisms of HSP70 K1, K3 and K4 PCR product were detected different patterns. In the experiment for AI of fresh semen, spring and fall season showed higher litter size born of 10.89 head than 10.47 head of summer season. Landrace was showed higher litter size of 9.96 head than that of Duroc and Yorkshire(p<0.05). Stress relating PSS and HSP70 polymorphism of PSS-Normal, HSP70 K1-BB, K3-AB, K4-AA showd a highest litter size born of 10.97 head and litter size born alive of 10.69 head than that of the other polymorphisms(p<0.05). In the experiment for AI of frozen semen, effects of season and pig breeds were not showed for litter size born. The stress relating polymorphism of PSS-Carrier, HSP70 K1-BB, K3-BB, K4-AB showed highest litter size born of 11.29 head and litter size born alive of 10.82 head and PSS-Normal, HSP70 K1-BB, K3-AB, K4-AA showed the lowest litter size born of 8.48 head and litter size born alive of 7.33 head than that of the other polymorphisms(p<0.05). These results suggest that AI litter size born for the stress of forzen thawed semen may be affected by PSS and HSP70 polymorphism in pigs.

Relationship between Stress Gene Polymorphisms and Litter Size by AI in Pigs

Jin, H.J.,Kim, I.C.,Wee, M.S.,Yeon, S.H.,Kim, C.D.,Lee, S.S.,Cho, C.Y.,Cho, S.R.,Son, D.S.,Park, C.K. 韓國受精卵移植學會 2007 한국동물생명공학회지 Vol.22 No.4

This study was performed to investigate the relationship between PSS-HSP70 gene polymorphism and artificial insemination (AI) reproductivity in the pigs. The RFLP polymorphism of PSS and the SSCP polymorphisms of HSP70 K1, K3 and K4 PCR product were detected different patterns. In the experiment for AI of fresh semen, spring and fall season showed higher litter size born of 10.89 head than 10.47 head of summer season. Landrace was showed higher litter size of 9.96 head than that of Duroc and Yorkshire (p<0.05). Stress relating PSS and HSP70 polymorphism of PSS-Normal, HSP70 K1-BB, K3-AB, K4-AA showd a highest litter size born of 10.97 head and litter size born alive of 10.69 head than that of the other polymorphisms(p<0.05). In the experiment for AI of frozen semen, effects of season and pig breeds were not showed for litter size born. The stress relating polymorphism of PSS-Carrier, HSP70 K1-BB, K3-BB, K4-AB showed highest litter size born of 11.29 head and litter size born alive of 10.82 head and PSS-Normal, HSP70 K1-BB, K3-AB, K4-AA showed the lowest litter size born of 8.48 head and litter size born alive of 7.33 head than that of the other polymorphisms(p<0.05). These results suggest that AI litter size born for the stress of forzen thawed semen may be affected by PSS and HSP70 polymorphism in pigs.

The Effects of Stress Related Genes on Carcass Traits and Meat Quality in Pigs

H. J. Jin,B. Y. Park,J. C. Park,I. H. Hwang,S. S. Lee,S. H. Yeon,C. D. Kim,C. Y. Cho,Y. K. Kim,K. S. Min,S. T. Feng,Z. D. Li,C. K. Park,C. I. Kim 아세아·태평양축산학회 2006 Animal Bioscience Vol.19 No.2

The current study was conducted to investigate the relationship between stress related gene and meat quality in pigs. A total number of 212 three-way cross bred (Landrace-Yorkshire횞Duroc) and 38 Duroc were sampled from the Korean pig industry to determine genotype requency of porcine stress syndrome (PSS) and heat shock protein 70 kDa (HSP70) genes and their relationship with carcass traits and longissimus meat quality. Screen of HSP70 was performed by the single strand conformation polymorphism (SSCP) technique. Based on the analysis of restriction fragment length polymorphism (RFLP) in ryanodine receptor 1 (RYR1) gene, genetic disorder of PSS was related to a mutation at 18,168th (C to T) of exon 17. There was no significant difference in ultimate meat pH and backfat thickness between HSP70 K1-AA type and -BB type in pure Duroc breed. In Landrace-Yorkshire횞Duroc (L-YxD) cross bred pig, our results indicated that HSP70 derivate type in Duroc had a limited effect on backfat thickness, but L-YxD type had a noticeable linkage with HSP70 K1-AA and K3-AB. This tendency was also observed in hot carcass weight where HSP70 K1-AA and K3-AB resulted in heavier weight with 86.3 kg compared to HSP70 K1-AB and K3-BB of 74.3 kg. Results imply that stress related HSP70 genotype has a potential association with backfat thickness and carcass weight.

Antiviral phenolics from the leaves of Cleistocalyx operculatus

Ha, T.K.Q.,Dao, T.T.,Nguyen, N.H.,Kim, J.,Kim, E.,Cho, T.Oh.,Oh, W.K. Elsevier 2016 Fitoterapia Vol.110 No.-

<P>During the screening program for anti-influenza agents from medicinal plants, the ethanolic extract of Cleistocalyx operculatus leaves was found to exhibit potential neuraminidase (NA) inhibitory activity. Bioassay directed fractionation led to the isolation of two new acetophenones (1 and 2) and one new flavanone (3), along with six known compounds (4-9). The structures of all isolated compounds were elucidated using various spectroscopic methods and through comparison with the previous literature. Compounds 6 and 8 exhibited strong enzymatic inhibition on various neuraminidases from different influenza viruses, including H1N1, H9N2, novel H1N1, and oseltamivir-resistant novel H1N1 (H274Y mutation) expressed in HEM293 cells (IC50 values ranging from 5.07 +/- 0.94 mu M to 9.34 +/- 2.52 mu M, respectively). Kinetic experiments revealed the non-competitive inhibitory mode of both compounds 6 and 8. Furthermore, these flavonoids reduced the cytopathic effect of the H1N1 virus in MDCK cells. The present study suggests the potential of two flavonoids (6 and 8) as new lead compounds for the development of novel NA inhibitors in the future. (C) 2016 Elsevier B.V. All rights reserved.</P>

k-NN 분류를 이용한 유압유 미립자 검출 기반 연구

최병희(B. H. Choi),조예찬(Y. C. Jo),박정현(J. H. Park),최성운(S. W. Choi),조상훈(S. H. Cho),김바다(B. D. Kim),박기협(K. H. Park),김주호(J. H. Kim),이대엽(D. Lee) 유공압건설기계학회 2023 유공압건설기계학회 학술대회논문집 Vol.2023 No.5

In many equipment, including construction machinery that operates with hydraulic oil, it is very important to solve the problem of the generation of fine particles in the hydraulic circuit and the inability to use the equipment due to causes such as damage of parts and repair. In this study, as a base study to solve these problems, a study that can detect the color change of hydraulic oil due to particles mixed in hydraulic oil in real time using machine learning was conducted. Using the k-NN classification algorithm, the color change of hydraulic oil is classified into ten classes so that the color can be recognized in real time. It is planned to carry out the development of the sensor system in the following study.

Attenuation of oxidative neuronal cell death by coffee phenolic phytochemicals

Cho, E.S.,Jang, Y.J.,Hwang, M.K.,Kang, N.J.,Lee, K.W.,Lee, H.J. Elsevier 2009 Mutation research Vol.661 No.1

Neurodegenerative disorders such as Alzheimer's disease (AD) are strongly associated with oxidative stress, which is induced by reactive oxygen species (ROS) including hydrogen peroxide (H<SUB>2</SUB>O<SUB>2</SUB>). Recent studies suggest that moderate coffee consumption may reduce the risk of neurodegenerative diseases such as AD, but the molecular mechanisms underlying this effect remain to be clarified. In this study, we investigated the protective effects of chlorogenic acid (5-O-caffeoylquinic acid; CGA), a major phenolic phytochemical found in instant decaffeinated coffee (IDC), and IDC against oxidative PC12 neuronal cell death. IDC (1 and 5μg/ml) or CGA (1 and 5μM) attenuated H<SUB>2</SUB>O<SUB>2</SUB>-induced PC12 cell death. H<SUB>2</SUB>O<SUB>2</SUB>-induced nuclear condensation and DNA fragmentation were strongly inhibited by pretreatment with IDC or CGA. Pretreatment with IDC or CGA also inhibited the H<SUB>2</SUB>O<SUB>2</SUB>-induced cleavage of poly(ADP-ribose) polymerase (PARP), and downregulation of Bcl-X<SUB>L</SUB> and caspase-3. The accumulation of intracellular ROS in H<SUB>2</SUB>O<SUB>2</SUB>-treated PC12 cells was dose-dependently diminished by IDC or CGA. The activation of c-Jun N-terminal protein kinase (JNK) and p38 mitogen-activated protein kinase (MAPK) by H<SUB>2</SUB>O<SUB>2</SUB> in PC12 cells was also inhibited by IDC or CGA. Collectively, these results indicate that IDC and CGA protect PC12 cells from H<SUB>2</SUB>O<SUB>2</SUB>-induced apoptosis by blocking the accumulation of intracellular ROS and the activation of MAPKs.

Solubility of oxcarbazepine in eight solvents within the temperature range T=(288.15-308.15)K

Nam, K.,Ha, E.S.,Kim, J.S.,Kuk, D.H.,Ha, D.H.,Kim, M.S.,Cho, C.W.,Hwang, S.J. Academic Press 2017 The Journal of chemical thermodynamics Vol.104 No.-

In this study, the solubility of oxcarbazepine in pure methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, acetone, acetonitrile, and tetrahydrofuran was analysed across the temperature range of 288.15-308.15K under atmospheric pressure by using a solid-liquid equilibrium method. The experimental values obtained data were correlated using the modified Apelblat model at each temperature. The mole fraction solubility of oxcarbazepine in all eight pure solvents increased gradually in a temperature-dependent manner. The highest mole fraction solubility of 3.08x10<SUP>-3</SUP> at 308.15K was observed for tetrahydrofuran, followed by acetone (1.82x10<SUP>-3</SUP> at 308.15K), acetonitrile (1.22x10<SUP>-3</SUP> at 308.15K), methanol (1.11x10<SUP>-3</SUP> at 308.15K), ethanol (6.17x10<SUP>-4</SUP> at 308.15K), 1-butanol (6.17x10<SUP>-4</SUP> at 308.15K), 1-propanol (6.16x10<SUP>-4</SUP> at 308.15K), and 2-propanol (4.13x10<SUP>-4</SUP> at 308.15K). The experimental solubility in all solvents correlated well with that calculated using the modified Apelblat equation across the temperature range of (288.15-308.15)K. Therefore, the experimental solubility and correlation equations established in this study could be useful during the crystallization/purification, pre-formulation, and formulation stages of oxcarbazepine production in laboratories and related industries.

A study on Driving of 35W(T5) fluorescent lamp by the electronic ballast using piezoelectric transformer

L H Hwang,J H Yoo,H S Song,S K Na,H S Kim,H S Oh,S H Lee,K H Choi,M T Cho 전력전자학회 2007 ICPE(ISPE)논문집 Vol.- No.-

Recently, 35W class Fluorescent lamps with 32㎜ tube diameter are replaced with 32W one with 26 ㎜ in diameter to conserve lamp materials and to increase luminance efficiency. Moreover, 35, 28 and 14 W fluorescent lamps with 16㎜ (T5) in diameter, which are nowadays developed, also may replace 32 W lamps again. Application of slim lamps, however, requires small sized electronic ballast to full fill the design philosophy of miniaturizing. However, the traditional magnetic ballasts operated at 50-60㎐ have been suffered from noticeable flicker, high loss, large crest factor and heavy weight. In this study, in order to solve these problems, a new type of electronic ballast, which is composed of rectifier, active power factor corrector, series resonant half bridge inverter and piezoelectric transformer, was proposed for driving T5 fluorescent lamp. Driving of piezoelectric transformer was carried out with input region for the ring electrode and output region for the dot electrode. A 35W (T5) fluorescent lamp is successfully driven by the fabricated ballast with piezoelectric transformer. After driving the lamp using the proposed electronic ballast for 20 min, the input power factor and efficiency of ballast shown 0.95 and 86%, respectively, at operating frequency of 81 k㎐. And also, the output power and temperature rise of piezoelectric transformer showed 35.07W and 20.5 , respectively.

WO₃ nanofibers functionalized by protein-templated RuO₂ nanoparticles as highly sensitive exhaled breath gas sensing layers

Kim, K.H.,Kim, S.J.,Cho, H.J.,Kim, N.H.,Jang, J.S.,Choi, S.J.,Kim, I.D. Elsevier Sequoia 2017 Sensors and actuators. B, Chemical Vol.241 No.-

<P>In this work, a novel catalytic synthesis and functionalization method using apoferritin is used to fabricate RuO2 nanoparticles (NPs) loaded WO3 nanofibers (NFs) for potential diagnosis of diabetes. Catalytic ruthenium (Ru) NPs with very small average diameters of 1.8 +/- 0.9 nm were synthesized using apoferritin which is a hollow protein cage, and were easily functionalized on WO3 NFs by introducing electrospinning solution with W precursor and polyvinylpyrrolidone (PVP). As-spun Ru NPs-loaded W precursor/PVP composite NFs were calcined at 600 degrees C for 1 h in air atmosphere to achieve RuO2-functionalized WO3 NFs. The small size and uniform distribution of catalytic RuO2 NPs were well maintained due to hollow nature of apoferritin cages after calcination. The chemo-resistive sensors using RuO2-functionalized WO3 NFs showed significantly enhanced acetone (CH3COCH3) sensing response (R-air/R-gas = 78.61-5 ppm), which was 7.4 times higher than the response (R-air/R-gas =10.61-5 ppm) of pristine WO3 NFs at highly humid atmosphere (95% RH). In addition, the RuO2-functionalized WO3 NFs showed outstanding selectivity toward acetone gas in comparison with other gases such as hydrogen sulfide (H2S), toluene (C6H5CH3), ethanol (C2H5OH), pentane (C5H12), ammonia (NH3), hydrogen (H-2), and water vapor (H2O) at 5 ppm. These results represent potential feasibility for the detection of acetone in exhaled breath for diagnosis of diabetes. (C) 2016 Elsevier B.V. All rights reserved.</P>