Effect of ER signal peptides (KDEL) on expression and function of anti-virus monoclonal antibody in plant

Da-Young Park,Yangkang So,Kyung-Jin Lee,Zhe Lu,Eun-Yi Oh,Kwang-Wok Min,Seul-Ki Lee,Jung-Hwan Lee,Mi-Hyun Ahn,Mira Song,Doo-Byoung Oh,Youngkwan Kim,Kisung Ko 한국당과학회 2009 한국당과학회 학술대회 Vol.2009 No.1

Plant genetic engineering has led to the production of plant-derived mAb (mAbP), which provides a safe and economically feasible alternative to the current antibody expression systems. In this study, the expression levels of mAbP SO57 with or without ER-signal peptides (Lys-Asp-Gly-Leu;KDEL) in transgenic tobacco plants were analysed in transgenic plant. PCR and Reverse Transcription-PCR analyses showed existence of heavy and light chain genes of mAb with or without KEDL and their transcription in plant, respectively. Western blot showed that the expression levels of mAbP SO57 with KDEL were significantly higher than that without KDEL. Flow cytometry analysis showed that the Fc domains of both purified mAbP and mammalian-derived mAb have similar binding activity to the FcγRI receptor. High-performance liquid chromatography analysis showed that the mAbP SO57 with KDEL had glycan profile with both oligomannose and golgi type, whereas the mAbP SO57 without KDEL had only golgi type glycans. Neutralizing analysis with rabies virus CVS-11 showed the similar neutralizing activity between mAbP SO57 with and without KDEL. These results suggest that the potential of mAbP SO57 for rabies immunotherapy is regardless of plant specific glycan structures.

Interacelluar reprogramming of expression, glycosylation, and function of plant-derived anti-virus therapeutic monoclonal antibody

Da-Young Park,Kyung-Jin Lee,Yangkang So,Zhe Lu,Seulki Lee,Jung-Hwan Lee,Seung Han Oh,Yeon Soo Han,Doo-Byoung Oh,Youngkwan Kim,Kisung Ko 한국당과학회 2010 한국당과학회 학술대회 Vol.2010 No.1

Plant genetic engineering has led to production of plant-derived mAb (mAbP), which can provides a safe and economically effective alternative to the conventional antibody expression methods. In this study, the expression levels of mAbP SO57 with or without ER-retention peptide extensions signal (Lys-Asp-Glu-Leu; KDEL) in transgenic tobacco plants were analysed in transgenic plant. The expression levels of mAbP SO57 with KDEL were significantly higher than that without KDEL regardless of transcription level. mAbP SO57 with KDEL localized surround to the nucleus suggesting that the mAbP with KDEL is localized in ER. The mAbP without KDEL and mAbH had mainly Golgi type glycans, whereas the ER-localized mAbP with KDEL had glycan profile with both oligomannose type (47.6%) and Golgi type (52.4%). The Fc domains of both purified mAbP (with and without KDEL) and human-derived mAb (mAbH) had similar bingding activity to the Fcγ RI receptor (CD64). Both mAbP (with and without KDEL) had a shorter half-life than mAbH. However, both mAbP with and without KDEL was as effective at neutralizing activity of the rabies virus CVS-11 as the mAbH. These results suggest that ER localization of recombinant mAbP by KDEL reprograms glycosylation and enhances production of the functional antivirus therapeutic antibody in the plant.

연령대별 선호 안경테와 안경원 인테리어에 대한 연구

한선희(Sun-Hee Han),김봉환(Bong-Hwan Kim),김소희(So-Hee Kim),주지윤(Ji-Yoon Joo),황슬아(Seul-Ah Hwang),박소연(So-Yeon Park),서현정(Hyun-Jung Seo),구수연(Soo-Yeon Koo) 한국안광학회 2019 한국안광학회지 Vol.24 No.2

Purpose: As the frequency of people wearing glasses has increased recently owing to the surge of different kinds of visual media, glasses are playing an important role not only in comforting the eye but also as a tool of fashion and personality. Therefore, we studied the age-wise preference pattern of the customers for the different types of glass frames and interior designs of optical shops. Methods: A questionnaire-based survey on preferred glass frames was conducted on 164 subjects (83 males and 81 females) visiting an optical shop in Ulsan. Another questionnaire-based survey on the interior designs of optical shops was conducted on 40 subjects (27 males and 13 females) by optometrists who work at 40 optical shops located at Jung-gu, Buk-gu, Nam-gu, and Dong-gu in Ulsan. Results: The survey showed that metal frames are preferred by individuals in their 10s, 20s, and 50s, whereas plastic frames are most preferred by individuals in their 30s and 40s. Among the different frame shapes, the round style is preferred by individuals in their 10s and 50s, the big frame style is preferred by individuals in their 20s and 30s, and the small frame style is preferred by individuals in their 40s. In addition, regarding the interior design of optical shops, subjects participating in the survey at Dong-gu preferred showcases, those participating at Nam-gu and Buk-gu preferred restrooms, and those participating at Jung-gu preferred refraction rooms. Conclusions: We anticipate that the sales can be augmented by improvising a better sales strategy in which the optometrists need to invest in altering the interior designs of the optical shops and pre-arranging the products according to the age-wise preference of the customers visiting the shops.

국내 하천 퇴적물 건강성평가를 위한 Microtox 독성시험 조건확립 연구

정홍배,박정규,문성환,류태권,김소정,배철한,황인영 한국환경독성학회 2001 환경독성보건학회지 Vol.16 No.4

1. 하천 퇴적물 공극수, 증류수 추출용액 및 유기용매 추출액을 pH6.0~6.5 범위로 조절한 후 Microtox 독성을 측정하는 것이 퇴적물내의 암모니아 독성이 배제된, 유해화학물질 오염에 의한 퇴적물 독성을 평가하는 방안임을 확인하였다. 2. 퇴적물에 대해 공극수, 증류수 추출물, 유기용매 추출물 구분하여 Microtox 독성을 구하는 것이 퇴적물 내 유해화학물질의 독성발현 특성을 예측할 수 있는 기법이라고 사료된다. 3. 조사정점 내 시료 채취 지점간 독성에 편차로 인하여, 동일 정점의 반경 50~100m 내에서 최소 4지점 이상의 퇴적물을 확보하는 것이 해당 정점의 독성 대표값을 산출할 수 있는 방안이 된다고 판단된다. 4. 한강수계인 남한강 지천인 복하천과 양화천, 금강수계인 청주 미호천, 대전 갑천, 낙동강수계인 대구 금호강, 영산강수계인 황룡강 그리고 익산 만경강을 대상으로 한 예비 조사한 결과, 집중연구대상 하천으로의 조건은 금호강이 가장 우수하였다. 5. 하천 퇴적물의 증류수 추출액과 유기용매 추출액의 Microtox 독성값 간의 상호 상관성이 높았다. 그러나, 농축 과정이 용이하므로 정점간 비교를 위한 독성 측정시 유기용매 추출액을 사용하는 것이 바람직하다고 사료되었다. Six rivers were selected as preliminary screening sites to determine the test conditions of Microtox in assessing the toxicity of the sediment. In addition, a pH range of 6.0--6.5 was established in testing pore water, aqueous extracts and organic extracts. Each extractable fraction of sediment showed different toxicities. Therefore, in order to properly examine the toxicity in the sediment, all extractable fractions of sediment samples needed to be tested with Microtox. Thus, sediment samples were additionally collected from at least 4 secondary sites within 50~100 m area of the primary sampling site to reduce any variation or deviation in toxicity assessment. From all sediment toxicity data that was collected from this study, it was concluded that the Keumho river was the most polluted with the highest sediment toxicity of all the rivers analyzed and needed further detailed research on its pollution problem.

유방수술환자의 성역할정체감에 따른 자아개념 적응

소희영,김정환 충남대학교 의과대학 지역사회의학연구소 1992 충남의대잡지 Vol.19 No.2

The present study was designed to investigate the influence of sex role identity on adaptation after breast surgery, and to intervene on self concept for improving adaptation process by understanding & describing about psychological and emotional aspects of who had breast surgery. Self concept is the one of 4 adaption form including physical, personal, and interpersonal self according to Roy's adaption model. The subjects in this study were 107 patients being operated at general surgery deparment of Chungnam University Hospital in Daejon. Data was collected at postoperative 7-10th day using the Short Form Bem Sex Role Identity Iventory, Jacox & Stewart's Health Self Concept, Beck's Depression Inventory, Zung SPAS. and Osgood's Semantic Differential Method from Mar. 1989 to Feb. 1992. The data were analysed by use of frequency, ANOVA, t-test, Pearson Correlation Coefficients of SPSS program. The results of the study were as follows 1. There was no significant difference of degree of health self concept, depression, anxiety, body image according to sex role identity. 2. Among the demographic characteristics of subjects, level of health self concept was statistically significant difference in age(F=5.1349, P=.0007), marital periods(F=4.4120, P=0058), and educational level(F=8.4928, P=0004), level of depression was statistically significant difference in educational level(F=5.0424, P=0081), levle of anxiety was statistically significant difference in age(F=8.0675, P=0006), marital status(t=-2.40, p.018), marital periods(F=3.1459, P=.0283), and educational level (F=3.2507, P=.0427), and level of body image was statistically significant difference in age(F=7.7233, P=0007), marital periods(F=2.8781, P=.0369), and occupation(t=-1.99, 3. There was no significant correlation between health self concept, depression, anxiety, and body image. Considering these survey results, there is no discrimination of self concept according to sex role identity of the patient after breast surgery. This means, cancer diagnosing it self and surgical experience it self are might be conceived as much more influence on patient than the sex role identity. Accordingly, we could see that sex role identity is not affect the self concept(adaption) after breast surgery. When we do as a psycho-emotional nursing intervention for the patient of breast surgery, they could be positive and undistressed self concept during adaption process if we approach her with demographic characteristics rather than sex role identity.

말티즈견에서 발생한 승모판폐쇄부전 1례

전소분,박혜연,김선아,정금선,이영원,박성준,김덕환,송근호 충남대학교 수의과대학 동물의과학연구소 2004 動物醫科學硏究誌 Vol.12 No.-

A 10 years old, intact male, Maltese dog with clinical signs of labored respiration, exercise intolerance and increasing of coughing was referred to the Veterinary Medical Teaching Hospital of Chungnam National University. Physical examination revealed tachycardia, panting and mild cyanosis. This case was diagnosed as mitral valve insufficiency by clinical signs, ascultation, blood chemistry, radiography and electrocardiography. This dog was treated with ACE inhibitor (enalapril), diuretics (furosemide) and bronchodilator (aminophylline) for 14 days. After treatment, clnical signs were disappeared and also this dog was needed to be continuous monitoring and sodium restricted diet.

LP강판의 두께변화에 따른 강거더의 효율성 평가

양경환,정경섭,이종선,권소진 충북대학교 건설기술연구소 2003 建設技術論文集 Vol.22 No.1

There has been an increased interest in the Longitudinally Profiled(LP) plate as a structural element for bridges, due to its advantage to efficiently distribute the sectional force applied to the structure. The advantage also includes the reduction of weight and the number of welding points. The LP plate was originally developed in Europe, and the application has since increased rapidly on steel bridges in Europe and Japan. Though widely used internationally, much of the study for the structural application of the LP plate is yet to be performed. In this paper, the workability of the LP plate was evaluated by the tests for tensile and bending. In addition, the stability was reviewed and verified by a comparison between experimental and analytical results. The economical efficiency was also examined for different section types by finite element analysis.

Wavelet 변환 영역에서 특징을 이용한 영상 검색 시스템의 구현

정소영,정성환 國立 昌原大學校 精報通信硏究所 1999 精報通信論文集 Vol.3 No.-

For the efficient image retrieval, we propose a retrieval method in wavelet transform domain, using color and texture feature. After performing the 2-level wavelet transform on Hue value of image, we extract color feature in low-level subband and texture feature in other subbands. And then, we use the extracted features as index keys. We retrieve the candidate images from the image database by matching the index key of a query image with the ones of images in the database. We implemented the proposed image retrieval system in WWW Client/Server environment. In the experimental results using 250 images, the proposed method shows about 93%, 82% in term of Recall and Precision.

간세포암환자에서 간동맥 화학 색전술 후 발생한 리피오돌에 의한 폐렴 1예

김소이,김유리,허현미,배서은,이명원,최윤정,김고흔,김태헌,유 권,문일환 이화여자대학교 의과대학 2009 EMJ (Ewha medical journal) Vol.32 No.2

Hepatocellular carcinoma(HCC)is one of common causes of cancer-related death in Korea where the majority of HCC patients were Hepaitc B virus(HBV)carriers and have cirrhosis. Transarterial chemoembolization(TACE)is commonly applied to the treatment of multinodular HCC in Korea and careful selection of candidate is important for the risk of various side effects. Besides common side effects as fever, nausea, abdominal pain and elevation of liver enzyme, TACE may predispose to hepatic failure, ischemic cholecystitis, pulmonary embolism, cerebral embolism and pneumonitis. In previous studies, some cases of pulmonary and cerebral embolism cases were reported but lipiodol pneumonitis after TACE was rarely reported. A 65-year-old woman with a multinodular HCC associated with HBV infection, was treated with TACE. Seven days after the procedure, nonspecific respiratory symptoms such as dyspnea and dry cough developed. Chest X-ray and chest computed tomography showed diffuse ground glass opacities in whole lung fields, suggestive of lipiodol pneumonitis. After several days of supportive care with steroid administration, radiologic abnormalities and subjective symptoms were much improved, considered that the disease was compatible with lipiodol pneumonitis.

내용기반 영상검색을 위한 칼라와 질감 특징 추출

정소영,정성환 昌原大學校 基礎科學硏究所 1997 基礎科學硏究所論文集 Vol.9 No.-

For the content-based image database retrieval, we proposed a hierarchical retrieval method in transform domain using color and texture feature. First, we extract color feature in low-level subband of wavelet transform, and then retrieve the images within color boundary by the color feature. Next, we extract texture feature in other subbands for the hierarchical retrieval, and retrieve the candidate images from the images within color boundary. In the experimental results using 200 images, the performace of proposed method is better than Tamura's method.