금식시 흰쥐 소장 점막 유당분해효소에 대한 갑상선 호르몬의 영향 및 이의 유전적 조절 기전

이국래,이동호,이한주,윤정환,김재규,정운태,정현채,송인성,김정룡 大韓消化器病學會 1996 大韓消化器病學會誌 Vol.28 No.6

수용성 항진균성 항생물질을 생산하는 방선균 LAM-593의 분리 및 물질생산조건

이동희,김중배,이노운 建國大學校 附設 産業技術硏究所 1993 논문집 Vol.18 No.-

토양에서 분리 선별한 수용성 항진균성 물질을 생산하는 Streptomyces속 균주 LAM-593에 의한 항진균성 물질의 생산조건을 검토한 결과는 다음과 같다. 물질생산을 위한 탄소원으로는 soluble starch가, 질소원으로는 soybean meal과 peptone이 가장 우수하였으며 glycerol의 첨가로 물질 생성량이 현저히 증가하였으나 인산염에 의해서는 저해되었다. 생산 최적온도와 초발 pH는 각각 30℃와 9.5였으며, soluble starch 1.0%, glycerol 1.0%, soybean meal 0.3%, peptone 0.1%, MgSO₄·7HO 0.05%, NaCI 0.05%, silicone oil 0.01% 조성의 배지(pH 9.5) 2.5L를 5L fermenter에 넣고 전배양한 배양액을 3% 수준으로 접종하여 통기량 0.9vvm, 교반속도 200rpm으로 30℃ 에서 2일간 배양하였을 때 비지내의 항진균성 물질의 축적량이 최대에 달하였다. Cultural conditions for production of water soluble antifungal antibiotic by Streptomyces sp, strain LAM-593, isolated from soil, were studied. For the antibiotic production, soluble starch and soybean meal was favorable as carbon and nitrogen source, respectively. The antibiotic production was stimulated by the addition of Mg? and glycerol. Optimal temperature and initial pH for production of the antibiotic was 30℃ and pH 9.5, respectively. Accumlation of the antibiotic in th culture broth reached at maximum level after 2 days cultivation under aeration rate 0.9 vvm and impeller speed 200rpm in 5L-fermenter with 3L of medium containing soluble starch 1.0%, glycerol 1.0%, soybean meal 0.3%, peptone 0.1%, MgSO₄·7H₄O 0.05% and Nacl 0.05%.

제주도산 가무락, Cyclina sinensis의 생식주기에 관한 조직학적 연구

이정재,조운삼 제주대학교 해양연구소 1985 해양자원연구소연구보고 Vol.9 No.-

티타늄 레이저 용접부의 기계적 성질 평가

이중재,김길수,안승근,배태성,박찬운 大韓齒科器材學會 2003 대한치과재료학회지 Vol.30 No.2

This study was performed to examine the effects of laser output energy on the mechanical properties of laser-welded titanium joints. Titanium rods of 3 ㎜ in diameter were perpendicularly cut to 30 ㎜ in length for the laser welding. Two rods were fixed to each other with a jig and welded vertically using a Nd:YAG laser at output current of 210-270 A. The penetration depth of the laser beam against the titanium was measured with the changes of output current, pulse duration, and spot diameter. Mechanical tests included tensile testing and Vickers microhardness of the joints. Tensile testing was performed at the crosshead 00speed of 1.0 ㎜/min and a gauge length of 30 ㎜ with a universal testing machine. Vickers microhardness was mesured under 9.8 N load for 15 seconds. The results obtained were summarized as follows; With increasing the output current, the penetration depth of the laser to titanium was increased. Tensile strength values of laser-welded groups were the relatively higher than that of non-welded parent material group. There were significant differences in tensile strength between the specimens of laser-welded at output currents 250 A, 270 A and non-welded parent material group(p<0.05). Microhardness values were increased in the heat-affected zone. Brittle fracture regions were observed in the specimens of laser-welded at output currents 270 A.

박막 크로멜-알루멜 열전퇴형 교류-직류 변환기

정인식,김호운,김진섭,이정희,이종현,신장규,박세일,권성원 경북대학교 센서기술연구소 1998 센서技術學術大會論文集 Vol.9 No.1

A planar chromel-alumel multijunction ac-dc converter was fabricated on a LPCVD Si_(3)N_(4)/SiO_(2)/Si_(3)N_(4)-diaphragm, prepared by silicon bulk micromachining, which thermally isolated a bifilar evanohm-heater and the hot junctions of a chromel-alumel thermopile from the silicon substrate. The voltage responsivity, the ac-dc transfer error, and the fluctuation of the output thermoelectric voltage of the converter were investigated. The respective voltage responsivities in air and in a vacuum of the converter were about 3.16 mV/mW and 9.41 mV/mW. The ac-dc voltage and the current transfer errors in air were about ±1.5 ppm and ±0.7 ppm in a frequency range from 10 Hz to 10 kHz, respectively. The fluctuation of the output thermoelectric voltage from 5 seconds to 120 seconds after preheating in air for 5 seconds was about 0.06 % for a heater input of dc 1 V.

Hall 소자를 이용한 승강기용 로프의 평가

이종오,윤운하,손영호,김정우,이종규 한국비파괴검사학회 2003 학술대회 논문집 Vol.- No.1

홀 소자를 이용한 누설자속 측정으로 승강기용 와이어 로프의 결함 검출에 대한 연구를 수행하였다. 소선파단, 마모, 내부 소선파단 형태의 인공결함을 제작하여 시험편에 영구자석으로 자장을 걸어 결함에서 누설되는 누설자속을 홀 센서를 사용하여 검출하였다. 로프의 소선은 0.5, 0.8mm 두 종류로 시험결과 단선모델 시험에서 0.4mm, 내부의 소선 파단의 경우 1mm 정도 깊이를 가진 인공결함까지 검출이 가능하여 1개의 소선 파단도 검출할 수 있는 것으로 보인다. 마모모델에서 결함의 폭이 작은 경우 검출이 불가능하였으나, 폭이 4mm이상인 경우 자극이 결함을 통과할 때 자속의 변화에 의해 깊이가 0.2mm 정도의 작은 결함도 구별이 가능하였다. 따라서 Hall 센서에 의한 누설자속 측정이 wire rope의 결함 검출에 좋은 도구가 된다고 본다. Defect detection of wire rope for an elevator was investigated through the measurement of magnetic flux leakage. The types of defect usually found in wire rope categorized such as inner and outer wire breakage and wear. The specimens that has artificial defects were magnetized via permanent magnet, and measurement of magnetic flux leakage on the defects was performed with Hall sensor. In wire broken model, a defect smaller than 0.4mm and 1mm in depth on outer and inner wire rope, respectively, could be detected well. In wear model, smaller defect could not be detected clearly, however, appearance of changing of total magnetic flux during magnetic pole of the sensor passing through a defect 0.2mm in depth at 4mm or above width could make possible to detect it. From the results, the measurement via Hall sensor might be useful tool for defect detection of wire rope.

Canine Distemper Virus 검출을 위한 정량 Real-time PCR

이승관,이창규,정운원,조경진,김현숙,강영대,서장훈 高麗大學校 倂設 保健大學 保健科學硏究所 2002 保健科學論集 Vol.28 No.2

Canine distemper virus(CDV) infection causes a fatal systemic disease in wide range of carnivore species, including domestic dogs. It has been a highly contagious disease of veterinary importance for centurise. Recently many researches show that qualitative RT-PCR for the detection of CDV was sensitive and specific method for the early and safe antemortem diagnosis of distemper by using whole blood regardless of clinical sign and pathological finding. Recently, results were correlated to clinical findings, anti-CDV neutralizing antibody titers, postmortem findings, and demonstration of CDV NP(nucleoprotein) antigen by immunohistochemistry. We have studied a quantitative real-time PCR assay for CDV in order to improve and broaden the clinical usefulness of RT-PCR assay. Total 37 samples including 9 clinically positive and 29 randomly selected ones have investigated. CDV RNA was extracted from whole blood. The isolated RNA was reverse-transcripted into cDNA followed by PCR amplification. The assay based on real-time PCR using SYBR Green I dye as quantitative method with a LightCycler. Calibration curves covered 30-3×10^(8) copies of CDV were created using cloned 240bp CDV cDNA of viral RNA. CDV was detected all in 8 positive samples whereas 8 in 29 randomly collected ones. The detection ranges of clinical CDV specimens were various from 3×10² to 3×10⁴ copies. Comparision of real-time PCR to conventional PCR showed equal sensitivity and specificity. But real-time PCR method has a merit of quantification as well as more rapid results than those of common RT-PCR. CDV can be accurately detected and quantified in the whole blood from the dog infected with CDV. This quantitative technique may improve the clinical utility of RT-PCR for the detection of CDV.

DNA Microarray를 이용한 인유두종 바이러스(Human Papilloma Virus:HPV)의 진단

정운원,이승관,이창규,조경진,김성욱 高麗大學校 倂設 保健大學 保健科學硏究所 2001 保健科學論集 Vol.27 No.1

Human papillomavirus(HPV) has been known as one of the important pathogenic agent in uterine cervical carcinoma. The molecular works such as PCR enable the detection of large number of HPV genotypes obtained from viginal swab. Many of the PCR-based methods for HPV detection involve an amplification step followed by any of a number of methods for distinguishing different HPV types. In this study, we adopted the DNA chip technology enabling a HPV type-specific differentiation both low-risk group(type-6, 11, 34, 40, 42, 43, and 44) and high-risk group(type-16, 18, 31, 33, 35, 39, 45, 51,52,54, 56, and 58). MY09/MY11 and GP5+/GP6+primers covered LI region are used in nested PCR to improve PCR amplification. HPV type-specific probes for DNA chip were modified with NH2-C6, followed by spotting on silylated slides, washing slides and hybridization with each PCR products. Of 163 DNA samples chosen randomly, 42 samples were negative, 8 ones for low-risk group of HPV and 96 ones for high-risk group of HPV. Especially, co-infections with various HPVs were shown in 17 samples. A recent study found that multiple HPV is a factor in persistent HPV infection, resulting in the development of cervical dysplasia. This result emphasized the necessity to detect multiple HPV infection. The application of DNA-chip to determinate specific HPV typing will be a stronger candidate than any other PCR-based methods. Furthermore, the sequencing data of the positive PCR products were shown no discrepancy with DNA chip results. This means that DNA chip is very useful tool for both HPV detection and typing.

부산광역시의 대기질 변화경향 및 특성에 관한 연구

이화운,김유근,원경미,홍정혜,문윤섭,장난심,정우식 부산대학교 환경문제연구소 1997 環境硏究報 Vol.15 No.1

스포츠 센터 수영장의 경영관리(1)

이동운,이정섭,조연철 계명대학교 생활과학연구소 2000 科學論集 Vol.26 No.-

This is research results which can service to technique study of administration management and groping for method which can much intensify function of facilities, according to the introduction of new sports facilities which can be sufficient needs of consumer and conversion. First, The local residents' opinion has to be reflected to make the most of the swimming pool in sports center thus preferentially it is taken enough consideration not only space guarantee but also arrangement of facilities. second, It doesn't have any difference in the scale of Taegu downtown many swimming pool facilities under residents' disposition. It showed that the scale of change room and shower bath are influenced in Business sales. the last thing is especially, it showed that The scale of sauna facilities have an effect on Business.