장내의 탄수화물과 지방 흡수 억제를 통한 체지방 및 비만 개선 효과에 관한 연구

정은희,윤승원,이홍석,윤유식,유경미,황인경 한국조리과학회 2003 한국식품조리과학회지 Vol.19 No.1

In a previous study, a dietary supplement was developed in our lab using natural herbal extracts against digest enzyme activity in GI tract for weight control. This natural herbal extracts could regulate absorption of glucose and lipid by the inhibition of digest enzyme activity. In this study, we screened the natural herbs that inhibit glucoamylase activity and developed an water extract of cinnamon. The cinnamon extract delayed and decreased the increment of carbohydrate degradation through the inhibition of glucoamylase activity in vitro. Fifty volunteers were subjected to the intake of the herbal extracts by taking twice a day for 60 days. As a result, the treated subjects lost 3kg of body weight and 3.5kg of body fat mass after the treatment. Furthermore, the body mass index and waist size were significantly decreased during the experimental period. Above results suggested that the administration of the dietary additives composed of cinnamon and natural herbal extract improves the obesity by the decrement of body weight and body fat mass.

廢棄物埋立地 浸出水에서 黃酸鹽還元菌의 分布와 重金屬 不溶化役割

정권,신재영,정일현,고견택일유,유영식 한국환경위생학회 1997 한국환경보건학회지 Vol.23 No.3

This study, collaborated Gifu University. Japan, was performed to analyze chemical pollutants and microorganism and to clarify the distribution of sulfate-reducing bacteria and their insolubilization of heavy metal ions in leachates sampled seasonally between 1994 and 1996 from Nanjido waste landfill site, sampled 4 times between 1995 and 1996 from Pusan and Daejeon waste landfill site. and sampled 1 time between 1992 and 1994 from Hokkaido. Nagoya, Osaka and Hukuoka waste landfill site in Japan. The results were as follows: 1. The temperatures of internal leachate and leachate effluent were 40℃ and 30℃, respectively, and the pH values of both leachates were about 8.0 at Nanjido waste landfill site. The concentration of SO_(4)^(-2)gradually increased with the degree of stabilization and that of NO_(3)-N was detected in a part of sampling sites at one and half years, and in all sampling sites at 3 years after completion of landfill. 2. The organic substances in leachate of Nanjido waste landfill site decreased with the degree of stabilization and they were very fluctuated with measuring point and time. The concentration of organic substance and heavy metals in internal leachate were higher than in leachate effluent and those of Cd, Hg. and Pb were lower than detection limit except a part of samples in 1996. 3. APCs in internal leachate and leachate effluent were not much different and the minimum of APCs in internal leachate and leachate effluent were 1.0 x 10^(4)/ml and 4.0 x 10^(1)ml. respectively. 4. The maximums of SRBs in Nanjido, Pusan, and Daejeon waste landfill site were 9180 MPN/ml, 24000 MPN/ml, and 348 MPN/ml, respectively and the maximum of SRBs in Japan waste landfill site was 9300 MPN/ml. 5. During 2-week-SRB culture, the values of MPN were high at 50℃ for initial culture period and at 30℃ for last culture period. MPN started to appear at first day and rapidly increased between 7th day and 9th day. 6. Cadmium and copper were insolubilized by SRB within 6 hr and iron and zinc were done within 48 hr. The rates of insolubilization of Cd. Cu. Fe. Zn, T-Cr were 100%. 99.5%. 95.0%. 99.8%. 16.1% after 48 hr treatment with SRB. respectively.

환경친화형 수분산성 불소 아크릴레이트 공중합체에 의한 섬유 표면개질

유수용,김정두,문명준,서차수,주창식,이민규 한국환경과학회 2004 한국환경과학회지 Vol.13 No.10

Waterborne fluorinated acrylate copolymer (WFAC) for surface modification of textile was synthesized from perfluoroalkyl ethyl acrylate, octadecyl acrylate, glycidyl methacrylate, surfactant and 3,3 methyl-methoxy butanol. The structures of the synthesized WFAC were determinated by FT-IR and ^(19)F-NMR analysis. The thermal stability investigated with DSC and TGA was decreased with increasing the content of fluorinated acrylate in the copolymer. However, the particle sizes of WFAC were increased with increasing the content of fluorinated acrylate in the copolymer. The surface energies calculated by contact angles of WFAC were in the range of 29.80~3.41 dyne/cm. On the observing SEM of the textile surface treated with WFAC, the textile was swollen and compacted with increasing the concentration of water repellency agent. WFAC synthesized in this study showed a good water repellency.

3-Cyano 및 3-Carbomethoxy Chromone과 Allenylstannane과의 반응

鄭大一,宋有淨,崔舜圭,李龍均,安賢淑,윤구식 東亞大學校附設基礎科學硏究所 1998 基礎科學硏究論文集 Vol.15 No.2

t-Butyldimethylsilyl triflate 존재에서 3-Cyano 및 3-Carbomethoxy Chromone(1a,b)과 Allenylstannane(2) 과의 반응에서 Chromone의 C-2위치에 propargyl기가 도입된 유도체(3a,b)를 합성하였다. 합성한 유도체의 상대적 구조는 ¹H-NMR을 통해 분석하였으며, T.S구조의 모델을 통하여 가능한 메카니즘을 제시하였다. Reaction of 3-Cyano and 3-Carbomethoxy Chromone(1a,b) with Allenylstannane(2) in the presence of tert-butyldimethylsilyl triflate afforded the corresponding the propargylation product(3a,b) in the moderate yield. The relative stereochemistry of 3a,b are obtained on the basis of ¹H-NMR analysis. And the possible reaction mechanism are suggested by the molecular models in the transition state.

전분 충전 아크릴레이트 필름의 α-Amylase에 의한 생분해

김정두,유수용,감상규,주창식,이민규 한국환경과학회 2004 한국환경과학회지 Vol.13 No.9

The biodegradability of vinyl acetate acrylate resin and corn starch blend was studied by determination of the reduced sugars produced after enzymatic hydrolysis. The starch hydrolysis reaction by a-amylase was achieved within 5 minutes. Optimal ranges of temperature and pH for the starch hydrolysis by a-amylase were around 80 ℃ and 6.5-7.2, respectively. The biodegradability of the starch-filled acrylate films increased as the content of starch increased. The biodegradation of starch in the starch-filled acrylate film by a-amylase was about 48.6% of that of pure starch. This value of biodegradable starch-filled acrylate film gave a good result with enzymatic shortcut test. The surface morphologies of the starch-filled acrylate film after enzymatic hydrolysis were investigated by scanning electron microscopy (SEM).

비만 개선 효과를 지닌 탄수화물 및 지방 흡수 억제 기능성 식이조성물 개발

윤유식,최선미,홍순복,홍정미,김정원,이홍석,홍성길 한국조리과학회 2002 한국식품조리과학회지 Vol.18 No.3

In a previous study, we developed a new food additive as an egg yolk antibody (IgY) against carbohydrate digestion enzymes for the regulation of blood glucose level and weight control. The IgY delayed and decreased the increment of blood glucose level after administration of sucrose in human being by 30% in 20∼30 min. We also developed a lipase inhibitor as a water extract of two kinds of herb, Platycodon grandiflorum and Solanum melongena. Twenty three volunteers were subjected to the intake of the egg yolk IgY plus the herbal extracts for 50 days. In average, the treated subjects appeared to lose 1.96 ㎏ of body weight and 3.4 ㎏ of body fat mass during the treated period. Furthermore, Panniculus adiposus and breech size were significantly decreased during the experimental period. Above results suggested that the administration of the dietary additives composed of egg yolk IgY and natural herbal extract improve the obesity by the decrement of body weight and body fat mass.

합성 Goethite에 의한 인산이온, 황산이온 및 구리이온의 흡착 특성

김정두,유수용,문명준,감상규,주창식,이민규 한국환경과학회 2003 한국환경과학회지 Vol.12 No.9

Adsorption of phosphate, sulfate, and copper ion to goethite was investigated. Goethite was prepared in the alkaline solution. In the single adsorbate systems, the final equilibrium plateau reached within 20 min. The adsorption isotherms of the individual ions could be well described by the Langmuir equation. The maximum adsorption capacities (q_(max)) were calculated as 0.483 mmol/g and 0.239 mmol/g at pH 3 for phosphate and sulfate ion, and 0.117 mmol/g at pH 6 for copper ion, respectively. In competitive adsorption system with phosphate and sulfate, phosphate ion was a stronger competitor for adsorption on goethite than sulfate ion, which was consistent with higher affinity of phosphate ion for the surface compared to sulfate ion. The existence of sulfate ion enhanced the adsorption of copper ion but the adsorption of sulfate was inhibited when copper ion was present.

A Study on DNA Sequences and Mutation of Integrase Region of Korean-type Bovine Leukemia Virus (BLV) pol Gene

권오식,--,--,-- THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 2004 Journal of biomedical laboratory sciences Vol.10 No.1

Bovine leukemia virus (BLV) is a causative agent for lymphoma disease in cattle including cows worldwide. BLV shares similar virion structure and characteristics with other retroviruses. The pol gene of the BLV genome produced reverse transcriptase (RT) and integrase (IN) for important roles for BLV genome integration into host cell chromosomes that is known to be coded in the 3' side of the BLV pol gene (one third portion). In this study, we have sequenced 978 bp in the 3'side of the BLV pol gene from BLV 10C3 in order to determine the BLV IN region of it. And we compared it to the nucleotide sequences of an Australian BLV isolate. As a result, nucleotide sequences of the IN region of the Korean-type BLV pol gene were mutated at a rate of 3.7%. We can confirm that the typical mutations are such as Arg (AGG) → Lys(AAG), Thr (ACG) → Met (ATG), Ile (ATT) → Val (GTT), Asn (ACC) → His (CAC), Phe (TTT) → Leu (TTG) and Asn (ACC) → Asp (GAC). From the analysis of the sequencing data, we were able to determine the zinc-finger-like "HHCC" motif in the amino terminus of BLV IN, that was H-X_(3)-H-X_(25)-C-X_(2)-C. It was also found the DD35E motif in the IN catalytic domain as D-X_(56)-D-X_(35)-E. It fits very well to the consensus sequences of retroviral IN as well as HHCC motif.

주정중독 환자와 정상음주자에 대한 감정상태와 자기 지각, 가정환경, 사회적 지지에 관한 비교 연구

이정식,한광수,이유상,한일우,신용구 용인정신의학연구소 1997 용인정신의학보 Vol.4 No.1

마산연안지역의 국지풍 순환 및 대기오염물질 확산에 관한 연구

김유근,이화운,문윤섭,김해동,원경미,정우식,오인보 부산대학교 환경문제연구소 1997 環境硏究報 Vol.15 No.1

To investigate a horizontal windrose and the mixing height in Masan coastal area, a local atmospheric flow and the concentration of air pollutants were analyzed by abserved data from AWS, airsonde and air sampler. The land-sea breeze model and the ISCST2 model of U. S. EPA(Environmental Protection Agency) were used to predict the concentration of air pollutants such as SO2 and TSP. As a result, during spring the principal wind direction in the Masan region is mainly represented by windrose of the WNW and NW, which can be induced by land breeze and the SE and SSW of sea breeze even if weak wind speed. The mixing height during the measurement was marked with the range from 400 m(0900 LST) to 1450 m(1500 LST), and the height of an inverse layer was presented as the range from 30 m(2100 LST) to 150 m(0300 LST). It is very important to cosider the local wind field for the accurate diffusion and movement of air pollutants in coastal urban area. The atmospheric flow was simulated by the two-dimensional local wind model upon considering the orographic characteristics such as land-sea contrast, mountain valley and urban building. The result of air quality simulated by ISCST2 support that the dispersion pattern of the pollutants in that area was followed as local circulation system in coastal urban area.