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- 저자 : Junda Liu (검색결과 3 건)
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Transcriptome analysis of the livers of ducklings hatched normally and with assistance
Yali Liu,Shishan He,Tao Zeng,Xue Du,Junda Shen,Ayong Zhao,Lizhi Lu 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.6
Objective: “Hatchability” is an important economic trait in domestic poultry. Studies on poultry hatchability focus mainly on the genetic background, egg quality, and incubation conditions, whereas the molecular mechanisms behind the phenomenon that some ducklings failed to break their eggshells are poorly understood. Methods: In this study, the transcriptional differences between the livers of normally hatched and assisted ducklings were systematically analyzed. Results: The results showed that the clean reads were de novo assembled into 161,804 and 159,083 unigenes (≥200-bp long) by using Trinity, with an average length of 1,206 bp and 882 bp, respectively. The defined criteria of the absolute value of log2 fold-change ≥1 and false discovery rate≤0.05 were differentially expressed and were significant. As a result, 1,629 unigenes were identified, the assisted ducklings showed 510 significantly upregulated and 1,119 significantly down-regulated unigenes. In general, the metabolic rate in the livers of the assisted ducklings was lower than that in the normal ducklings; however, compared to normal ducklings, glucose- 6-phosphatase and ATP synthase subunit alpha 1 associated with energy metabolism were significantly upregulated in the assisted group. The genes involved in immune defense such as major histocompatibility complex (MHC) class I antigen alpha chain and MHC class II beta chain 1 were downregulated in the assisted ducklings. Conclusion: These data provide abundant sequence resources for studying the functional genome of the livers in ducks and other poultry. In addition, our study provided insight into the molecular mechanism by which the phenomenon of weak embryos is regulated.
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He Dongxin,Zang Qingjing,Li Junda,Liu Hongshun,Xu Zhe,Li Qingquan 대한전기학회 2023 Journal of Electrical Engineering & Technology Vol.18 No.5
The thermal stress at the interface of different materials becomes distorted during the thermal expansion of a cable terminal, leading to cracking and insulation failure. To give a warning of the ultimate thermal stress of cable terminal, this paper presents a warning method for the ultimate thermal stress of the cable terminal based on tailpipe temperature monitoring. Taking a 110 kV gas-insulated switchgear cable terminal as an example, the heat transfer model of the cable terminal is established under the working condition of a through-flow. The relationship between the temperature of the tailpipe, maximum thermal stress of the epoxy casing, and current-carrying capacity of the cable is established based on a data fitting method, the ultimate stress of the epoxy is taken as a threshold value for the detection and calculate the corresponding current-carrying capacity of the cable and temperature of the tailpipe. The results show that the thermal stress concentration in the cable terminal exceeds the limit when the monitoring temperature at the exposed tailpipe reaches 54.25 °C. If the relationship between the external temperature of the cable terminal and maximum thermal stress is known, warning of the ultimate thermal stress of cable terminal by monitoring the temperature of the tailpipe, providing theoretical support for the regulation of the current-carrying capacity of the cable.
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