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Reduced Graphene Oxide-Based Assay for Real-Time Monitoring of Cancer Cell Viability
Jing Wu,Yang Tang,Zengliang Shi,Zhengshan Tian,Junhua Wang,Chi Yang,Jitao Li,Zhongdang Xiao,Chunxiang Xu 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2015 NANO Vol.10 No.7
Viability of cancer cell is an important indicator of physiological state and function of cells, which can be effected by the change of pH in the medium solution, due to the increase of carbon oxide and lactic acid caused by respiration. Although many methods have been developed to detect the viability of cells, mostly based on cytochemical staining and polymerase chain reaction (PCR) technology are time consuming. In this paper, an electronic device was made by thermal reduced graphene oxide (RGO) for detection of cancer cell viability in real-time. This electronic device could be used to monitor the metabolic activity and viability of cancer cells based on the change in pH value. As the pH decreases, colon cancer cells loose viability and the current decreases. This RGO device is simple, sensitive and label-free and could serve as a platform for detection of cells and drug testing.
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Liu, HongLing,Wu, JunHua,Min, Ji Hyun,Hou, Peng,Song, Ah-Young,Kim, Young Keun IOP Pub 2011 Nanotechnology Vol.22 No.5
<P>The Fe<SUB>3</SUB>O<SUB>4</SUB>–Ca<SUB>3</SUB>(PO<SUB>4</SUB>)<SUB>2</SUB> core–shell nanoparticles were prepared by one-pot non-aqueous nanoemulsion with the assistance of a biocompatible triblock copolymer, poly(ethylene glycol)-<I>block</I>-poly(propylene glycol)-<I>block</I>-poly(ethylene glycol) (PEO–PPO–PEO), integrating the magnetic properties of Fe<SUB>3</SUB>O<SUB>4</SUB> and the bioactive functions of Ca<SUB>3</SUB>(PO<SUB>4</SUB>)<SUB>2</SUB> into single entities. The Fe<SUB>3</SUB>O<SUB>4</SUB> nanoparticles were pre-formed first by thermal reduction of Fe(acac)<SUB>3</SUB> and then the Ca<SUB>3</SUB>(PO<SUB>4</SUB>)<SUB>2</SUB> layer was coated by simultaneous deposition of Ca<SUP>2 + </SUP> and PO<SUB>4</SUB><SUP>3 − </SUP>. The characterization shows that the combination of the two materials into a core–shell nanostructure retains the magnetic properties and the Ca<SUB>3</SUB>(PO<SUB>4</SUB>)<SUB>2</SUB> shell forms an hcp phase (<I>a</I> = 7.490 Å, <I>c</I> = 9.534 Å) on the Fe<SUB>3</SUB>O<SUB>4</SUB> surface. The magnetic hysteresis curves of the nanoparticles were further elucidated by the Langevin equation, giving an estimation of the effective magnetic dimension of the nanoparticles and reflecting the enhanced susceptibility response as a result of the surface covering. Fourier transform infrared (FTIR) analysis provides the characteristic vibrations of Ca<SUB>3</SUB>(PO<SUB>4</SUB>)<SUB>2</SUB> and the presence of the polymer surfactant on the nanoparticle surface. Moreover, the nanoparticles could be directly transferred to water and the aqueous dispersion–collection process of the nanoparticles was demonstrated for application readiness of such core–shell nanostructures in an aqueous medium. Thus, the construction of Fe<SUB>3</SUB>O<SUB>4</SUB> and Ca<SUB>3</SUB>(PO<SUB>4</SUB>)<SUB>2</SUB> in the core–shell nanostructure has conspicuously led to enhanced performance and multi-functionalities, offering various possible applications of the nanoparticles.</P>
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The standards of obstetrics and gynecology core outcome sets: A scoping review
Shi Jiyuan,Gao Ya,Wu Shuang,Niu MingMing,Chen Yamin,Yan Meili,Song Ziwei,Feng Hui,Zhang Junhua,Tian Jinhui 한국한의학연구원 2022 Integrative Medicine Research Vol.11 No.1
Background: Core outcome sets (COSs) are the minimum outcomes which should be measured and reported by researchers investigating a specific condition. The definition of standards of COSs vary across different health-related areas. This investigated the characteristics of COSs regarding obstetrics and gynecology (OG) and examined the reports and designs of standards of OG COSs. Methods: A comprehensive search was conduced on the COMET database on December 20, 2019 to identify systematic reviews on COSs. Two reviewers independently evaluated whether the reported OG COS met the reporting requirements as stipulated in the Core Outcome Set-STAndards for Reporting (COS-STAR) statement checklist and the minimum design recommendations as outlined in the Core Outcome Set-STAndards for Development (COS-STAD) checklist. Results: Forty-four OG COSs related to 26 topics were identified. None of them met all the 25 standards of COS-STAR statement which representing 18 items considered essential for transparent and complete reporting list for all COS studies (range: 6.0-24.0, median: 14.0). The compliance rates to 16 standards of methods and result sections ranged from 27.3%–68.2%. Total COS-STAR compliance items for OG COSs with the prior protocol was significantly higher than without prior protocol (MD = 3.846, 95% CI: 0.835–6.858, P = 0.012). None of the OG COSs met all the 12 criteria in the COS-STAD minimum standards (range: 3.0-11.0, median: 5.0). The compliance rates for all three standards of stakeholders involved and all four standards of the consensus process were lower than 60%. Conclusions: Methodological and reporting standards of OG COSs should be improved. Background: Core outcome sets (COSs) are the minimum outcomes which should be measured and reported by researchers investigating a specific condition. The definition of standards of COSs vary across different health-related areas. This investigated the characteristics of COSs regarding obstetrics and gynecology (OG) and examined the reports and designs of standards of OG COSs. Methods: A comprehensive search was conduced on the COMET database on December 20, 2019 to identify systematic reviews on COSs. Two reviewers independently evaluated whether the reported OG COS met the reporting requirements as stipulated in the Core Outcome Set-STAndards for Reporting (COS-STAR) statement checklist and the minimum design recommendations as outlined in the Core Outcome Set-STAndards for Development (COS-STAD) checklist. Results: Forty-four OG COSs related to 26 topics were identified. None of them met all the 25 standards of COS-STAR statement which representing 18 items considered essential for transparent and complete reporting list for all COS studies (range: 6.0-24.0, median: 14.0). The compliance rates to 16 standards of methods and result sections ranged from 27.3%–68.2%. Total COS-STAR compliance items for OG COSs with the prior protocol was significantly higher than without prior protocol (MD = 3.846, 95% CI: 0.835–6.858, P = 0.012). None of the OG COSs met all the 12 criteria in the COS-STAD minimum standards (range: 3.0-11.0, median: 5.0). The compliance rates for all three standards of stakeholders involved and all four standards of the consensus process were lower than 60%. Conclusions: Methodological and reporting standards of OG COSs should be improved.
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Autofluorescence generation and elimination: a lesson from glutaraldehyde
Lee, Kwahun,Choi, Sungmoon,Yang, Chun,Wu, Hai-Chen,Yu, Junhua The Royal Society of Chemistry 2013 Chemical communications Vol.49 No.29
<P>Glutaraldehyde causes especially high autofluorescence. It reacted with proteins and peptides to generate visible to near-IR emitters. A model indicated that ethylenediamine and a secondary amine in the molecule were key components for the formation of emissive species. The mechanism enables us to control the generation and elimination of autofluorescence.</P> <P>Graphic Abstract</P><P>Ways to control the generation and elimination of autofluorescence formed between glutaraldehyde and biomolecules. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c3cc40799c'> </P>
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Yanqiu Chen,Jin Zhang,Peishi Yu,Jian Wu,Weibo Wang,Junhua Zhao 한국강구조학회 2020 International Journal of Steel Structures Vol.20 No.4
The stress and strain concentration in some component has been a signifi cant topic for structural safety assessment. However, the evaluation of the stress and strain concentration based on the widely existing solutions for linear elastic material under static loading would lead to remarkable errors when the material undergoes the dynamic loading, since the coupling eff ect induced by the nonlinearity of the stress–strain relationship and the strain-rate strengthening could be of signifi cant infl uence. Here we analyzed the strain-rate dependent concentration of stress and strain for a visco-plastic plate with an elliptic hole subjected to dynamic loading. The three-dimensional stress and strain are calculated by fi nite element analyses based on a rate-dependent Johnson–Cook model in which the material constants are set according to a typical visco-plastic standard 45 carbon steel. Our results show that both the plastic stress and strain concentration factors signifi cantly depend on not only the strain but also the strain rate. With the increment of the remote strain rate, the stress concentration monotonically increases, while the strain concentration shows decreasing tendency.
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PENG AN,FANG ZUO,XINHUA LI,YUANPENG WU,JUNHUA ZHANG,ZHAOHUI ZHENG,XIAOBIN DING,YUXING PENG 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2013 NANO Vol.8 No.6
A biomimetic and facile approach for integrating Fe3O4 and Au with polydopamine (PDA) was proposed to construct gold-coated Fe3O4 nanoparticles (Fe3O4@Au–PDA) with a core–shell structure by coupling in situ reduction with a seed-mediated method in aqueous solution at room temperature. The morphology, structure and composition of the core–shell structured Fe3O4@Au–PDA nanoparticles were characterized by transmission electron microscopy (TEM), X-ray powder diffraction (XRD) and X-ray photoelectron spectrometry (XPS). The formation process of Au shell was assessed using a UV-Vis spectrophotometer. More importantly, according to investigating changes in PDA molecules by Fourier transform infrared spectroscopy (FTIR) and in preparation process of the zeta-potential data of nanoparticles, the mechanism of core–shell structure formation was proposed. Firstly, PDA-coated Fe3O4 are obtained using dopamine (DA) self-polymerization to form thin and surface-adherent PDA films onto the surface of a Fe3O4 "core". Then, Au seeds are attached on the surface of PDA-coated Fe3O4 via electrostatic interaction in order to serve as nucleation centers catalyzing the reduction of Au3+ to Au0 by the catechol groups in PDA. Accompanied by the deposition of Au, PDA films transfer from the surface of Fe3O4 to that of Au as stabilizing agent. In order to confirm the reasonableness of this mechanism, two verification experiments were conducted. The presence of PDA on the surface of Fe3O4@Au–PDA nanoparticles was confirmed by the finding that glycine or ethylenediamine could be grafted onto Fe3O4@Au–PDA nanoparticles through Schiff base reaction. In addition, Fe3O4@Au–DA nanoparticles, in which DA was substituted for PDA, were prepared using the same method as that for Fe3O4@Au–PDA nanoparticles and characterized by UV-Vis, TEM and FTIR. The results validated that DA possesses multiple functions of attaching Au seeds as well as acting as both reductant and stabilizing agent, the same functions as those of PDA.
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Liu, Kai,Ng, Joseph Kee-Yin,Wang, Junhua,Lee, Victor C. S.,Wu, Weiwei,Son, Sang Hyuk IEEE 2016 IEEE transactions on intelligent transportation sy Vol.17 No.6
<P>Vehicle-to-vehicle/vehicle-to-infrastructure (referred to as V2X) communications have potential to revolutionize current road transportation systems with respect to vehicle safety, transportation efficiency, and travel experience. This paper puts the first effort on applying network coding in cooperative V2X communication environments to improve bandwidth efficiency and enhance data service performance. Specifically, we investigate new arising challenges on network-coding-assisted data dissemination by considering both communication constraints and application requirements in vehicular networks. We present the system model and give an insight into the characteristics of cooperative data dissemination with network coding. On this basis, we formulate the problem and propose a network-coding-assisted scheduling algorithm to enable the hybrid of vehicle-to-vehicle (V2V) and vehicle-to-infrastructure (V2I) communications and exploit their joint effects on providing efficient data services. We design a cache strategy that allows vehicles to retrieve their unrequested data items. This strategy not only increases the opportunity of data sharing among vehicles but also gives higher probability of packet decoding, which in turn enhances the data service performance. We give an intensive analysis on the scheduling overhead, which shows the scalability of the algorithm. Finally, we build the simulation model and conduct a comprehensive performance evaluation to demonstrate the superiority of the proposed solution.</P>
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The synthesis and characterization of polymer-coated FeAu multifunctional nanoparticles
Liu, HongLing,Hou, Peng,Zhang, WengXing,Kim, Young Keun,Wu, JunHua IOP Pub 2010 Nanotechnology Vol.21 No.33
<P>We report the one-pot nanoemulsion synthesis of FeAu magnetic-optical multifunctional nanoparticles coated by the biocompatible triblock copolymer, poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol) (PEO-PPO-PEO). The FTIR study confirms the PEO-PPO-PEO molecules on the surface of the resulting nanoparticles. The structural characterization identifies the crystallographic parameter 4.072 Å of the cubic phase and the morphology analysis gives the nanoparticle shape, size and size distribution, showing the high crystallinity of the FeAu nanoparticles and an average particle size of ∼ 6.5 nm. In addition there is direct confirmation of the alloying by elemental point probing of an individual nanoparticle. Following the visual demonstration of a rapid, efficient and reversible dispersion–collection process of the nanoparticles in solution, the magnetic measurement manifests a soft ferromagnetic behavior of the nanoparticles with a small coercivity of ∼ 60 Oe at room temperature. The corresponding magnetic hysteresis curves were effectively assessed by modified bi-phase Langevin equations, which were satisfactorily explained in terms of a bimodal particle size distribution. The UV–vis studies display the broadband absorption of the PEO-PPO-PEO-coated nanoparticles with the maximum surface plasmon resonance around 585 nm. The characterization and analysis, therefore, shows the unification of iron and gold into one alloy nanostructure entity covered by the biocompatible triblock copolymer thin film, preserving the optical and magnetic properties of the individual constituents. This gives the prospect of enhanced performance in applications. </P>
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