자돈에 있어 게르마늄 용질액의 급여가 성장 및 혈액학적 변화에 미치는 영향

홍종욱,권오석,민병준,조진호,진영걸,손경승,강종옥,김인호 한국국제농업개발학회 2004 韓國國際農業開發學會誌 Vol.16 No.4

본 연구의 목적은 이산화게르마늄을 함유한 게르마늄 용질액을 자돈에 급여하였을 때 성장 및 혈액학적 변화에 미치는 영향을 조사하기 위하여 실시하였다. 3원 교잡종 자돈 60두 (평균체중 11.22±0.10㎏)를 공시하였다. 시험설계는 옥수수-대두박 위주의 대조구(CON)와, 대조구 사료내 게르마늄 용질액을 0.5 ppm 첨가한 구(GC0.5) 및 대조구 사료내 게르마늄 용질액을 1.0 ppm 첨가한 구(GC1.0)로 3개 처리를 하였다. 0-10일간 사양시험기간동안, 일당증체량에 있어서 GC0.5 처리구가 약간 높게 평가되었다(quadratic effect, P<0.02). 그러나 전체 시험기간동안, 일당증체량, 일당사료섭취량 및 사료효율에 있어서 처리구간에 유의적인 차이는 보이지 않았다. 영양소 소화율에 있어서는 GC0.5 처리구가 조회분(linear effect, P<0.01; quadratic effect, P<0.04), 칼슘(linear effect, P<0.01; quadratic effect, P<0.01), 인 (linear effect, P<0.02; quadratic effect, P<0.04) 소화율이 유의적으로 높게 평가되었다. 혈액내 neutrophil 농도에 있어서는 게르마늄 용질액의 첨가 수준이 증가함에 따라 유의적으로 증가하는 경향을 나타내었다(quadratic effect, P<0.02). 결론적으로, 자돈 사료내 게르마늄 용질액의 급여가 성장 및 혈액학적 수치에는 영향을 미치지 못하는 것으로 사료된다. This study was conducted to investigate the effects of dietary germanium colloid on the growth performance and hematological values in nursery pigs. Sixty pigs (11.22(0.10 ㎏ average initial body weight) were used in a 20 d growth assay. Dietary treatments included 1) CON (basal diet), 2) GC0.5 (basal diet+0.5 ppm germanium colloid) and 3) GC1.0 (basal diet+1.0 ppm germanium colloid). For d 0 to 10, pigs fed GC0.5 diet grew faster than pigs fed CON and GC1.0 diets (Quadratic effect, P<0.02). However, through the entire experimental period, no statistical differences were found for aver-age daily gain, average daily feed intake and gain/feed. Apparent digestibilities of crude ash (Linear effect, P<0.01; Quadratic effect, P<0.04), calcium (Linear effect, P<0.01; Quadratic effect, P<0.01) and phosphorus (Linear effect, P<0.02; Quadratic effect, P<0.04) in pigs fed GC0.5 diet were greater than for pigs fed CON and GC1.0. Neutrophil concentration in blood increased as the concentration of germanium colloid in the diets was increased with significant difference (Quadratic effect, P<0.02). In conclusion, growth performance and hematological values were not influenced by dietary germanium colloid.

Production and Characterization of Ethanol- and Protease-Tolerant and Xylooligosaccharides-Producing Endoxylanase from Humicola sp Ly01

( Jun Pei Zhou ),( Qian Wu ),( Rui Zhang ),( Yu Ying Yang ),( Xiang Hua Tang ),( Jun Jun Li ),( Jun Mei Ding ),( Yan Yan Dong ),( Zun Xi Huang ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.6

This paper reports the production and characterization of crude xylanase from the newly isolated Humicola sp. Ly01. The highest (41.8 U/ml) production of the crude xylanase was obtained under the optimized conditions (w/v): 0.5% wheat bran, 0.2% KH2PO4, and 0.5% peptone; initial pH 7.0; incubation time 72 h; 30℃; and 150 rpm. A considerable amount of the crude xylanase was induced using hulless barley bran or soybean meal as the carbon source, but a small amount of the enzyme was produced when supplementary urea was used as the nitrogen source to wheat bran. The crude xylanase showed apparent optimal cellulase-free xylanase activity at 60℃ and pH 6.0, more than 71.8% of the maximum xylanase activity in 3.0-30.0% (v/v) ethanol and more than 82.3% of the initial xylanase activity after incubation in 3.0-30.0% (v/v) ethanol at 30℃ for 2 h. The crude xylanase was moderately resistant to both acid and neutral protease digestion, and released 7.9 and 10.9 μmol/ml reducing sugar from xylan in the simulated gastric and intestinal fluids, respectively. The xylooligosaccharides were the main products of the hydrolysis of xylan by the crude xylanase. These properties suggested the potential of the crude enzyme for being applied in the animal feed industry, xylooligosaccharides production, and high-alcohol conditions such as ethanol production and brewing.

A Genetic Variant in MiR-146a Modifies Digestive System Cancer Risk: a Meta-analysis

Li, Ying-Jun,Zhang, Zhen-Yu,Mao, Ying-Ying,Jin, Ming-Juan,Jing, Fang-Yuan,Ye, Zhen-Hua,Chen, Kun Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.1

MicroRNAs (miRNAs) negatively regulate gene expression and act as tumor suppressors or oncogenes in oncogenesis. The association between a single nucleotide polymorphism (SNP) in miR-146a rs2910164 and susceptibility to digestive system cancers was inconsistent in previous studies. In this study, we conducted a literature search of PubMed to identify all relevant studies published before August 31, 2013. A total of 21 independent case-control studies were included in this updated meta-analysis with 9,558 cases and 10,614 controls. We found that the miR-146a rs2910164 polymorphism was significantly associated with decreased risk of digestive system cancers in an allele model (OR=0.90, 95%CI 0.87-0.94), homozygote model (OR=0.84, 95%CI 0.77-0.91), dominant model (OR=0.90, 95%CI 0.84-0.96), and recessive model (OR=0.85, 95%CI 0.79-0.91), while in a heterozygous model (OR = 0.99, 95% CI 0.89-1.11) the association showed marginal significance. Subgroup analysis by cancer site revealed decreased risk in colorectal cancer above allele model (OR=0.90, 95%CI 0.83-0.97) and homozygote model (OR=0.85, 95%CI 0.72-1.00). Similarly, decreased cancer risk was observed when compared with allele model (OR=0.87, 95%CI 0.81-0.93) and recessive model (OR=0.81, 95%CI 0.72-0.90) in gastric cancer. When stratified by ethnicity, genotyping methods and quality score, decreased cancer risks were also observed. This current meta-analysis indicated that miR-146a rs2910164 polymorphism may decrease the susceptibility to digestive system cancers, especially in Asian populations.

rs12904 Polymorphism in the 3'UTR of EFNA1 is Associated with Colorectal Cancer Susceptibility in a Chinese Population

Mao, Ying-Ying,Jing, Fang-Yuan,Jin, Ming-Juan,Li, Ying-Jun,Ding, Ye,Guo, Jing,Wang, Fen-Juan,Jiang, Long-Fang,Chen, Kun Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.9

Accumulated evidence has indicated that Ephrin A1 (EFNA1) is associated with angiogenesis and tumorigenesis in various types of malignancies, including colorectal cancer (CRC). In the current study, we performed an online search using the public microarray database to investigate whether EFNA1 expression might be altered in CRC tissues. We then conducted a case-control study including 306 subjects (102 cases and 204 well-matched controls) in Xiaoshan County to assess any association between genetic polymorphisms in EFNA1 and CRC susceptibility. Searches in the Oncomine expression profiling database revealed EFNA1 to be overexpressed in CRC tissue compared with adjacent normal tissue. The rs12904 G-A variant located in the 3' untranslated region (UTR) of EFNA1 was observed to be associated with CRC susceptibility. Compared with the AA homozygous genotype, those carrying GA genotype had a decreased risk of developing CRC (odds ratio (OR)=0.469, 95% confidence interval (CI): 0.225-0.977, and P=0.043). The association was stronger among smokers and tea drinkers, however, no statistical evidence of interaction between rs12904 polymorphism and smoking or tea drinking on CRC risk was found. Our results suggest that EFNA1 is involved in colorectal tumorigenesis, and rs12904 A>G polymorphism in the 3' UTR of EFNA1 is associated with CRC susceptibility. Larger studies and further mechanistic investigations are warranted to confirm our findings.

Research on Human-robot Shared Control of Throat Swab Sampling Robot Based on Intention Estimation

Ying-Long Chen,Fu-Jun Song,Peng-Yu Zhao,Yong-Jun Gong,Heng-Fei Yan 제어·로봇·시스템학회 2024 International Journal of Control, Automation, and Vol.22 No.2

With the spread and persistence of COVID-19, pharyngeal swab sampling, is an important link in nucleic acid testing, which is characterized by a high workload and susceptibility to infection. Therefore, it is necessary for medical workers to use medical robots instead of manual site sampling for collaborative sampling. However, the traditional teleoperation has difficulty ensuring the closed-loop performance due to the delay of the actual process, along with the weak control performance; Moreover, a robot cannot accurately plan and track sampling paths due to sensor accuracy and the changes in patient pharyngeal posture. The paper proposes a human-robot shared control strategy based on intention estimation, introducing the human intention as a reference, and the operator and robot work together to solve various significant problems during sampling. The human-robot negotiation based on the method includes the human judgement and perception and the robot into the invasion task. Through, the shared control based on the operator intention estimation, the robot can operate the obstacle avoidance and approach the target contact point remotely. Finally, two kinds of experiments of invasion process of throat swab sampling are implemented: a static target invasive experiment and a dynamic target invasive experiment, aiming at two different sampling conditions. Compared with the robotic independent control sampling, the time consumption in the two experiments is reduced by 34.8% and 41.6%, respectively, and the ultimate target position is basically within the scope of sampling field (where the range of the posterior pharynx wall < 20 mm). Thus, the sampling rate can reach 100%. Compared with independent control sampling by humans, the time consumption of the two experiments is respectively reduced by 15.9% and 42.3% on average, and the target position accuracy and sampling rate are quite close. Experimental results show that the control strategy improves the speed, flexibility, and intelligence of task execution compared to common sampling methods, laying the foundation for low-cost human-robot collaborative sampling.

Ginsenoside Production and Morphological Characterization of Wild Ginseng (Panax ginseng Meyer) Mutant Lines Induced by g-irradiation (^(60)Co) of Adventitious Roots

Jun-Ying Zhang,배태웅,Kyung-Hwan Boo,선현진,송인자,Chi-Hoa Pham,Markkandan Ganesan,양대화,강홍규,Suk-Min Ko,Key-Zung Riu,Pyung-Ok Lim,Hyo-Yeon Lee 고려인삼학회 2011 Journal of Ginseng Research Vol.35 No.3

With the purpose of improving ginsenoside content in adventitious root cultures of Korean wild ginseng (Panax ginseng Meyer),the roots were treated with different dosages of γ-ray (5, 10, 25, 50, 75, 100, and 200 Gy). The growth of adventitious roots was inhibited at over 100 Gy. The irradiated adventitious roots showed significant variation in the morphological parameters and crude saponin content at 50 to100 Gy. Therefore, four mutant cell lines out of the propagation of 35 cell lines treated with 50 Gy and 100Gy were selected on the basis of phenotypic morphology and crude saponin contents relative to the wild type control. The contents of 7 major ginsenosides (Rg_1, Re, Rb_1, Rb_2, Rc, Rf, and Rd) were determined for cell lines 1 and 3 from 100 Gy and lines 2 and 4from 50 Gy treatments. Cell line 2 showed more secondary roots, longer length and superior growth rate than the root controls in flasks and bioreactors. Cell line 1 showed larger average diameter and the growth rate in the bioreactor was comparable with that of the control but greater in the flask cultured roots. Cell lines 1 and 2, especially the former, showed much more ginsenoside contents than the control in flasks and bioreactors. Therefore, we chose cell line 1 for further study of ginsenoside contents. The crude saponin content of line 1 in flask and bioreactor cultures increased by 1.4 and 1.8-fold, respectively, compared to the control. Total contents of 7 ginsenoside types (Rg_1, Re, Rb_1, Rb_2, Rc, Rf, and Rd) increased by 1.8 and 2.3-fold, respectively compared to the control. Crude saponin and ginsenoside contents in the bioreactor culture increased by about 1.4-fold compared to that the flask culture.

Studies on Correlation of Chemical Composition of Jasmine Tea, with establishment of Multiple Linear Regression Equations for Sensory Quality

Jun Zhang,De-Song Tang,Shu-Ying Gong,Ying-Bin Zhang,Ping Chen,Zhi-Lei Gu 한국차학회 2015 한국차학회지 Vol.- No.S

The relationships among the concentrations of chemical compositions, scores of sensory quality and price were studied based on 112 jasmine tea samples. Linear correlation analysis showed that 5 quality attributes (appearance, liquor color, aroma, taste and infused leaves) were positively correlated with each other and to the sum. 5 quality attributes and total quality score were logarithm relevant to price with the coefficients of determination R2 were 0.855, 0.667, 0.836, 0.900, 0.906 and 0.914. The six taste attributes could be grossly divided into two groups. Tenderness, sweetness, freshness and mellowness were grouped showing positive correlation with taste score, total quality score and price, while heaviness and thickness were grouped for opposite correlation. There were positive correlation within the same group and negative correlation between two different groups. The concentrations of polyphenols, amino acid, water extract, gallocatechin (GC), epigallocatechin (EGC), catechin (C), epicatechin (EC), epigallocatechin gallate (EGCG), gallocatechin gallate (GCG) and epicatechin gallate (ECG) in tea infusions were negatively correlated with tenderness, sweetness, freshness, mellowness, taste score, total quality score and price but positively correlated with heaviness and thickness (except some individual components), while gallic acid (GA) was the opposite. Multiple linear regression equations of taste score and total quality score were established. The back substitution was of high correlation between predicted and actual value and acceptance rate was over 90%. The relationships among the concentrations of chemical compositions, scores of sensory quality and price were studied based on 112 jasmine tea samples. Linear correlation analysis showed that 5 quality attributes (appearance, liquor color, aroma, taste and infused leaves) were positively correlated with each other and to the sum. 5 quality attributes and total quality score were logarithm relevant to price with the coefficients of determination R2 were 0.855, 0.667, 0.836, 0.900, 0.906 and 0.914. The six taste attributes could be grossly divided into two groups. Tenderness, sweetness, freshness and mellowness were grouped showing positive correlation with taste score, total quality score and price, while heaviness and thickness were grouped for opposite correlation. There were positive correlation within the same group and negative correlation between two different groups. The concentrations of polyphenols, amino acid, water extract, gallocatechin (GC), epigallocatechin (EGC), catechin (C), epicatechin (EC), epigallocatechin gallate (EGCG), gallocatechin gallate (GCG) and epicatechin gallate (ECG) in tea infusions were negatively correlated with tenderness, sweetness, freshness, mellowness, taste score, total quality score and price but positively correlated with heaviness and thickness (except some individual components), while gallic acid (GA) was the opposite. Multiple linear regression equations of taste score and total quality score were established. The back substitution was of high correlation between predicted and actual value and acceptance rate was over 90%.

Plant regeneration of Korean wild ginseng (Panax ginseng Meyer) mutant lines induced by g-irradiation (60Co) of adventitious roots

Jun-Ying Zhang,Hyeon-Jin Sun,In-Ja Song,배태웅,Hong-Gyu Kang,Suk-Min Ko,Yong-Ik Kwon,김일웅,이재천,Shin-Young Park,Pyung-Ok Lim,Yong Hwan Kim,Hyo-Yeon Lee 고려인삼학회 2014 Journal of Ginseng Research Vol.38 No.3

An efficient in vitro protocol has been established for somatic embryogenesis and plantlet conversionof Korean wild ginseng (Panax ginseng Meyer). Wild-type and mutant adventitious roots derived fromthe ginseng produced calluses on Murashige and Skoog (MS) medium supplemented with 0.5 mg/L2,4-dichlorophenoxyacetic acid and 0.3 mg/L kinetin; 53.3% of the explants formed callus. Embryogeniccallus proliferation and somatic embryo induction occurred on MS medium containing 0.5 mg/L2,4-dichlorophenoxyacetic acid. The induced somatic embryos further developed to maturity on MSmedium with 5 mg/L gibberellic acid, and 85% of them germinated. The germinated embryos weredeveloped to shoots and elongated on MS medium with 5 mg/L gibberellic acid. The shoots developedinto plants with well-developed taproots on one-third strength Schenk and Hildebrandt basal mediumsupplemented with 0.25 mg/L 1-naphthaleneacetic acid. When the plants were transferred to soil, about30% of the regenerated plants developed into normal plants.

Age-Related CD4<SUP>+</SUP>CD25<SUP>+</SUP>Foxp3<SUP>+</SUP> Regulatory T-Cell Responses During Plasmodium berghei ANKA Infection in Mice Susceptible or Resistant to Cerebral Malaria

Ying Shan,Jun Liu,Yan-Yan Pan,Yong-Jun Jiang,Hong Shang,Ya-Ming Cao 대한기생충학열대의학회 2013 The Korean Journal of Parasitology Vol.51 No.3

Sentinel lymph Node mapping versus systematic pelvic lymphadenectomy on the prognosis for patients with intermediate-high-risk Endometrial Cancer confined to the uterus before surgery: trial protocol for a non-inferiority randomized controlled trial (SNE

Jun Guan,Yu Xue,Rong-yu Zang,Ji-hong Liu,Jian-qing Zhu,Ying Zheng,Bo Wang,Hua-ying Wang,Xiao-jun Chen 대한부인종양학회 2021 Journal of Gynecologic Oncology Vol.32 No.4

Background: Sentinel lymph node (SLN) mapping has been recommended as an alternative staging approach to lymphadenectomy for apparent uterine-confined endometrial cancer (EC). However, the prognostic value of SLN mapping alone instead of systematic lymphadenectomy on EC patients remains unclear. Methods: A multi-center, open label, non-inferiority randomized controlled trial has been designed to identify if SLN mapping alone is not inferior to pelvic lymphadenectomy on prognosis of patients with intermediate-high-risk EC clinically confined to uterus. Eligible patients will be 1:1 randomly assigned to accept SLN mapping or pelvic lymphadenectomy. The primary endpoint is the 2-year progression-free survival (PFS). The second points are the 5-year PFS, 5-year overall survival, surgery-related adverse events and life quality. A total of 780 patients will be enrolled from 6 hospitals in China within 3-year period and followed up for 5 years. Trial Registration: ClinicalTrials.gov Identifier: NCT04276532