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Nanomechanical Measurement of Bacterial Adhesion Force Using Soft Nanopillars
Oh, Naeun,Jun, Myeongjun,Lee, Jaejong,Eom, Chi-Yong,Park, Sungsu American Scientific Publishers 2017 Journal of Nanoscience and Nanotechnology Vol.17 No.11
<P>Bacteria adherent to the surface are implicated with biofilm formation and infection. Elastic polymer with tunable mechanical properties, such as PDMS (polydimethyl siloxane), has been used as a substrate for measuring traction force generated by animal cells. In this study, soft pillars (diameter: 900 nm, height: 2 mu m, stiffness: 24.1 nN/mu m) made of PDMS were used to measure adhesion force between Staphylococcus aureus and PDMS surface. The retraction of soft pillars upon S. aureus binding serves as a means for force estimation and is measured by change of the center-to-center (c-t-c) distance between pillars. Surprisingly, the c-t-c distance between two neighbouring pillars was decreased by 2.2% from its original distance upon bacterial adhesion onto the pillars. Based on the result, the average adhesion force of bacterial binding to pillars was calculated to 0.9 +/- 2 nN, which is much higher than expected. This suggests that the soft pillars can be used as a nanomechanical sensor for detecting various bacteria and could be a tool for sensing bacterial adhesion force in nano-newton range.</P>
Ketebo Abdurazak Aman,Park Chanyong,Kim Jaewon,Jun Myeongjun,Park Sungsu 나노기술연구협의회 2021 Nano Convergence Vol.8 No.19
Filamin A (FLNa) belongs to an actin-binding protein family in binding and cross-linking actin filaments into a three-dimensional structure. However, little attention has been given to its mechanobiological role in cancer cells. Here, we quantitatively investigated the role of FLNa by analyzing the following parameters in negative control (NC) and FLNa-knockdown (KD) U87 glioma cells using submicron pillars (900 nm diameter and 2 μm height): traction force (TF), rigidity sensing ability, cell aspect ratio, migration speed, and invasiveness. During the initial phase of cell adhesion (< 1 h), FLNa-KD cells polarized more slowly than did NC cells, which can be explained by the loss of rigidity sensing in FLNa-KD cells. The higher motility of FLNa-KD cells relative to NC cells can be explained by the high TF exerted by FLNa-KD cells when compared to NC cells, while the higher invasiveness of FLNa-KD cells relative to NC cells can be explained by a greater number of filopodia in FLNa-KD cells than in NC cells. Our results suggest that FLNa plays important roles in suppressing motility and invasiveness of U87 cells.