Oxidized LDL Accelerates Cartilage Destruction and Inflammatory Chondrocyte Death in Osteoarthritis by Disrupting the TFEB-Regulated Autophagy-Lysosome Pathway

Jeong Su Lee,Yun Hwan Kim,JooYeon Jhun,Hyun Sik Na,In Gyu Um,Jeong Won Choi,Jin Seok Woo,Seung Hyo Kim,Asode Ananthram Shetty,Seok Jung Kim,Mi-La Cho The Korean Association of Immunobiologists 2024 Immune Network Vol.24 No.3

Osteoarthritis (OA) involves cartilage degeneration, thereby causing inflammation and pain. Cardiovascular diseases, such as dyslipidemia, are risk factors for OA; however, the mechanism is unclear. We investigated the effect of dyslipidemia on the development of OA. Treatment of cartilage cells with low-density lipoprotein (LDL) enhanced abnormal autophagy but suppressed normal autophagy and reduced the activity of transcription factor EB (TFEB), which is important for the function of lysosomes. Treatment of LDL-exposed chondrocytes with rapamycin, which activates TFEB, restored normal autophagy. Also, LDL enhanced the inflammatory death of chondrocytes, an effect reversed by rapamycin. In an animal model of hyperlipidemia-associated OA, dyslipidemia accelerated the development of OA, an effect reversed by treatment with a statin, an anti-dyslipidemia drug, or rapamycin, which activates TFEB. Dyslipidemia reduced the autophagic flux and induced necroptosis in the cartilage tissue of patients with OA. The levels of triglycerides, LDL, and total cholesterol were increased in patients with OA compared to those without OA. The C-reactive protein level of patients with dyslipidemia was higher than that of those without dyslipidemia after total knee replacement arthroplasty. In conclusion, oxidized LDL, an important risk factor of dyslipidemia, inhibited the activity of TFEB and reduced the autophagic flux, thereby inducing necroptosis in chondrocytes.

Chlamydomonas CpSRP54 functions in assembly of light harvesting chlorophyll antenna complex

Jooyeon JEonG,Kwangryul BAEK,Inhwa SonG,EonSeon JIN 한국생물공학회 2017 한국생물공학회 학술대회 Vol.2017 No.4

Isolation and characterization of the γ-type carbonic anhydrase from Chaetoceros neogracile

Jooyeon JEONG,Hancheol JEON,Min Ju KIM,Kwang Suk CHANG,EonSeon JIN 한국생물공학회 2012 한국생물공학회 학술대회 Vol.2012 No.4

Strain engineering by using genome editing technology to improve algal biomass productivity

Jooyeon JEONG,Kwangryul BAEK,SangsSu BAE,EonSeon JIN 한국생물공학회 2016 한국생물공학회 학술대회 Vol.2016 No.10

A survey of attitudes to clinical practice guidelines in general and adherence of the Korea practical guidelines for management of gallbladder polyp

Jooyeon Jeong,Jae Keun Kim,Joon Seong Park,Dong Sup Yoon 한국간담췌외과학회 2014 한국간담췌외과학회지 Vol.18 No.2

Backgrounds/Aims: While clinical practice guidelines are effective tools for improving the quality of patient care and provide specific recommendations for daily practice, the usage of them have been often suboptimal. Therefore, evaluation of physician attitude to guidelines is an important initial step in improving guideline adherence levels. The aim of this study was to survey the attitude on general guidelines and adherence with the Korea Practical Guidelines for gallbladder (GB) polyp two year after their publication and distribution among Korean private clinicians. Methods: To evaluate the survey, questionnaires were sent with a stamp on an addressed envelope to 3,256 private clinicians who were registered at the Seoul Medical Association in April, 2010. From the 3,256 questionnaires, 376 clinicians (11.5%) responded to the survey. Results: A total of 91.0% responders agreed to the statement that general guidelines were useful tools for improving patient care and quality of care. One hundred one responders (26.9%) stated that they were aware of the Korea GB polyp guidelines while 73 physicians (72.3%) founded the guideline had changed their practice and user-friendly. Most of physicians (73.4%) agreed to practical procedures recommended by guidelines. Conclusions: Korean primary physicians were generally positive to the practical guidelines, as propagation of the guideline among primary physicians may improve adherence to guideline and patients care for GB polyps.

The Recent Evolution of Korean Enterprise Bargaining

Jooyeon Jeong 고려대학교 노동문제연구소 1997 노동연구 Vol.14 No.-

Deletion of the chloroplast LTD protein impedes LHCI import and PSI–LHCI assembly in <i>Chlamydomonas reinhardtii</i>

Jeong, Jooyeon,Baek, Kwangryul,Yu, Jihyeon,Kirst, Henning,Betterle, Nico,Shin, Woongghi,Bae, Sangsu,Melis, Anastasios,Jin, EonSeon Oxford University Press 2018 Journal of experimental botany Vol.69 No.5

<▼1><P>The <I>Chlamydomonas reinhardtii</I> LTD null mutant generated by CRISPR–Cas9, displayed aberrant PSI–LHCI holocomplexes, suggesting that the LTD protein may selectively function in PSI–LHCI assembly in green microalgae</P></▼1><▼2><P><B>Abstract</B></P><P>Nuclear-encoded light-harvesting chlorophyll- and carotenoid-binding proteins (LHCPs) are imported into the chloroplast and transported across the stroma to thylakoid membrane assembly sites by the chloroplast signal recognition particle (CpSRP) pathway. The LHCP translocation defect (LTD) protein is essential for the delivery of imported LHCPs to the CpSRP pathway in <I>Arabidopsis</I>. However, the function of the LTD protein in <I>Chlamydomonas reinhardtii</I> has not been investigated. Here, we generated a <I>C. reinhardtii ltd</I> (<I>Crltd</I>) knockout mutant by using CRISPR–Cas9, a new target-specific knockout technology. The <I>Crltd1</I> mutant showed a low chlorophyll content per cell with an unusual increase in appressed thylakoid membranes and enlarged cytosolic vacuoles. Profiling of thylakoid membrane proteins in the <I>Crltd1</I> mutant showed a more severe reduction in the levels of photosystem I (PSI) core proteins and absence of functional LHCI compared with those of photosystem II, resulting in a much smaller PSI pool size and diminished chlorophyll antenna size. The lack of CrLTD did not prevent photoautotrophic growth of the cells. These results are substantially different from those for Arabidopsis <I>ltd</I> null mutant, indicating LTD function in LHCP delivery and PSI assembly may not be as stringent in <I>C. reinhardtii</I> as it is in higher plants.</P></▼2>

기업 근로자의 표준에 대한 관심이 표준교육 수요에 미치는 영향 구조분석: 교육수준, 전문직여부의 조절효과를 중심으로

정주연(Jooyeon Jeong) 표준인증안전학회 2017 표준인증안전학회지 Vol.7 No.3

본 연구에서는 표준 관련 업무를 담당하는 기업의 근로자를 대상으로 표준에 대한 관심, 표준의 중요성 인식, 표준교육수요에 대해 조사하였다. 구체적으로 표준에 대한 관심, 표준의 중요성 인식, 표준교육수요가 기업 근로자의 사회․인구학적 특성에 따라 어떤 차이가 있는가를 조사하였다. 또한, 기업 근로자의 표준에 대한 관심, 표준중요성 인식, 표준교육 수요에 미치는 영향을 공변량구조 분석을 사용하여 검증하였다. 본 연구결과를 정리․종합하면 다음과 같다. 첫째, 표준에 대한 관심과 생활표준에 대한 관심, 표준의 중요성 인식이 표준교육수요에 미치는 영향을 구조분석을 통해 검증한 결과 근로자의 표준에 대한 관심과 생활표준에 대한 관심 수준이 높은 근로자 일수록 표준의 중요성에 대해 높게 인식하고 있는 것으로 나타났다. 또한 표준에 대한 관심과 생활표준에 대한 관심, 표준의 중요성에 대한 인식 수준이 높은 근로자일수록 표준교육에 대한 수요가 많은 것으로 나타났다. 둘째, 근로자의 교육수준 따라 표준관심, 생활표준관심, 표준중요성인식과 표준교육수요와의 관계에서 조절효과를 검증한 결과 근로자의 교육수준에 따른 일부경로에서 차이가 있는 것으로 나타났다. 그러나 근로자의 전문직여부에 따라서는 조절효과가 나타나지 않았다. This study investigated interest on standard, significance recognition on standard, need of education on standard with enterprise workers in related business. Concretely this study investigated differences by enterprise worker’s socio-demographic feature to interest on standard, significance recognition on standard, need of education on standard. Also, this study tested influencing factors of interest on standard, significance recognition on standard, need of education on standard by covariance structure analysis. The results are as follows. First, by testing interest on standard, interest on standard in life, and significance recognition on standard using analysis of structure, enterprise worker had high awareness on importance of standard if the worker has high interest on standard and standard in life. Also, this study showed high need of education on standard if the worker has high interest on standard, standard in life, and importance of standard. Second, testing by regulation effect on relationship between interest on standard, standard in life, importance of standard and need of education on standard using variable of worker’s education level, there was difference according to the education level’s path. However, there was no regulation effect according to specialized job.

국내 금속산업 기업별 노조

정주연(Jooyeon Jeong) 한국고용노사관계학회 2010 産業關係硏究 Vol.20 No.4

본 논문은 국내에서 지배적인 노조 유형인 기업별 노조의 구조 및 기능적 특성과 조합원의 이해를 대표하는 그 능력에서 나타난 한계를 다룬다. 이를 위해 일본을 중심으로 한 선진국들의 기업별 노조에 대한 문헌 검토를 통해서 분석틀을 도출한다. 이 틀을 기초로 국내의 금속산업의 3개 기업별 노조에서 노조 형성의 기원, 조직 원칙, 조합원의 특성, 조직의 안정성과 결정요인, 교섭 능력, 파업의 위력 등의 기준에서 일본과 비교해서 여러 공통점이나 차이점을 드러내고 분석했다. 국내에서 일본과 다른 점은 노조 형성의 기원에서 협력적인 노사관계를 지향하려는 사용자의 의지나 역할이 부족한 점, 재벌그룹차원의 기업별 노조의 연맹의 부재, 조직의 안정성이 사용자의 일방적인 경영전략의 변화에 의해 크게 약화된 점 등이다. 하지만 정규직 근로자 중심의 노조조직의 원칙, 조합원의 높은 사회 및 경제적 지위, 조직의 안정성의 결정에 기업적인 요소들이 중요함, 교섭이나 파업이 사전적이고 전략적인 대응이 아니라 사후적인 대처로 기업별 노조의 한계를 보인다는 것은 두 나라가 유사했다. This paper deals with the structural and functionl features of enterprise unionism as the prevailing union form in Korea as well as its limits in capacity for representation of interests of its rank-and-files. An analytical framework was derived from the literature review of enterprise unions in advanced nations including Japan. That framework illuminates and analyzes similarities and differences of three Korean enteprise unions in metal firms in comparison with the Japanese counterpart in criteria of origins of union formation, organization principles, features of rank-and-files, unions' organization security and its determinants, bargaining capacity, and dispute leverages. In Korea, significant differences were the lack of employers' willingness and roles to pursue cooperative relations with unions, the absence of enterprise union federations at chaebol-level, unilaterally employer-led managerial changes threatening union security. However, their similarities were observed in enterprise unions' organization principle exclusively organizing full-time employees, high economic and social statuses of rank-and-files in enterprise unions of large-sized firms, significance of firm-specific factors influencing organizational security of enterprise unions, limited bargaining and strike weapons owning reactive rather than proactive and strategic capacities.

표준 셀의 지엽적 이동을 통한 Metal2 단락 완화

정주연(Jooyeon Jeong),김태환(Taewhan Kim) 대한전자공학회 2020 대한전자공학회 학술대회 Vol.2020 No.11