독성물질의 세포사 기전 및 세포사 유발물질의 검색법 개발에 관한 연구(Ⅰ) : 독성물질로 인한 파킨슨병 모델에서의 세포사 기전 연구 Study on the cell-death mechanisms of toxin-induced parkinsonism

강태석,김종민,서경원,김영옥,김준규,오재호,이윤동,김규봉,오정자,송연정,임종준,전범석,문전옥,최광식 식품의약품안전청 2000 식품의약품안전청 연보 Vol.4 No.-

MPTP 독성물질이 도파민성 신경세포에 선택적으로 작용하여 산화성 손상에 의한 신경세포사를 일으키는 것을 이용하여 파킨슨병의 동물모델을 만들고, 이를 통해서 아폼토시스를 비롯한 포사의 기전에 대한 연구 및 너코틴의 신경세포 보호효과 여부를 판정하는 실험을 병행하고자 하였다. 파킨슨꾐의 동물모델을 MPTf 독성 물질을 이용하여 확립하였으며, MPTP(30mgag, i.p.)를 투여한 후 1, 2,3, 4, 5일째 흑질 조직을 채춰하여 tarm로 박걸하여 tyrosine hydroxylase 면역조직화학염색을 수행하여 cell countif우한 결과, control은 57.635ce11s, 1일째 친.OfDells,2일째 57.9±6cells,3일릴 없.3±죠ells, 4일째 49.0츠3cells, 5일째 39.4±Scells료 4, 3일째 뚜렷한 신경세포 수의 감소를 보였다. 신경세포사 기전 규명을 위한 아폼토시스 분걱에서는 벼PTP 투여 후 1, 2, 3, 4, 5일째 조직을 채취하여 Hoechst staining, TUNEL staining을 수곡하였는데 양성 반응을 보인 신경세포는 관찰되지 않아. 아폼토시스로 인한 세포사가 관찰되지 않았다. bIPTP 파킨슨병 동물모델에서 nicotine 보호효과 탐색에 관한 실험은 nicat푸e 0.2mgAg을 5일 퐁안 투여 후 리『fP(30mgag)를 CS7Bt/6 마은스에 복강 내주사로 nicotine과 병용 투여한 후 1, 2, 3, 4, 5일째 뇌를 적출하땄다. 신경세포사가 뚜렷이 관찰되기 시작하는 4, 5일째의 신경세포 수의 감소 정도를 20. 30% 정도 약화시키는 경향을 보였으나, nicotine 보호효과에 대한 추가 실헝이 현재 수행 중에 있다. The cause of Parkinson's disease (PD) is largely unknown. However, free radical toxicit? may plaf a role ip. the degeneration of substantia nigra, which is the Hajorfocus of pathological damages in PD. Recently, a neuroprotective effect of nicotine in PD has been suggested. Therefore, the mechanism of neurodegenerafion and protective potential o( nicotine in PD were investigated in the experimental modeB of Pll using a neurotoxin, C57BL/6mice were administered with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP, 30 mg/kg,j.p.). The degree of neurodegenerafion was determined by immunohistochemical stainiHB oftyrosine hydroxylase (TH). TH-positive cells on nigral sections were found 56.0 ±4, 57.9 ±6,52.315ce11s, 49.0±3cells, and 39,4±Scells at days 1, 2, 3, 4, 5, respectively (controls : 57.6±Scells). Hoechst and TUNEL staining showed no evidence of apoptosis. The exandnation on themice co-adrunistered with nicotine(0.2mgAg) and MPTP(30mgag) revealed a tendency ofnicotine protective effects. At days 4 and 5, the degree of TH-positive cells was decreased by20-30%, In corclusiffn, the role of apoptosis was not evidenced in this MPTP modeB of PB.The possible proteccon by nicotine should be elucidated with further studies.

[논문]실시간 정밀 3차원 지형측량기법을 이용한 저수량산출시스템 개발

송석진,장용구,김상석,강인준 釜山大學校生産技術硏究所 2004 生産技術硏究所論文集 Vol.63 No.-

현재 고정밀도 저수량 측량에 사용되는 측량장비에는 수평위치결정방법으로 실시간 DGPS측량장비가 많이 활용되고 있고, 수심측량에는 디지털 음향측심기를 많이 사용하고 있다. 본 연구에서는 현재, 수심측량 결과처리 시 문제가 되고 있는 소프트웨어의 가격부담을 줄이고 수심측량과 내업처리에 의한 저수량산출을 통합처리 할 수 있는 국내 자체개발의 저가형 외 · 내업 일체형 저수량산출시스댐을 개발하였다.

성인 여성의 골밀도와 관련된 요인

도준형,강복수,주리,김석범,김상규 한국모자보건학회 2000 한국모자보건학회지 Vol.4 No.2

前腕部 經穴 取穴에서 骨度分寸法과 一夫法의 比較 硏究

박히준,채윤병,차웅석,박종배,이혜정,이향숙,인창식,고형균,김수영,최일환,김강식,문정배,배기태,유경환,육근영,정병주,손인철,임사비나 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2004 東西醫學硏究所 論文集 Vol.2004 No.-

Background : The cun measurement System, an essential and convenient method in locating acupoints, has been widely used in the practice of acupuncture. However, traditional cun measurement has been criticized for its lack of reliability. Objectives : The purposes of this study are to determine if one cun measured by the directional methods have a consistency with that of proportional methods and to investigate which factors are related with these differences, especially in forearm. Methods : The distance between the elbow crease and the wrist crease of forearm was compared to a reference value of one cun obtained by the directional method. In this method, one cun is one third of the distance between index finger and Small finger of a subject, measured at proximal interphalangeal joint. In addition, to investigate the factors influencing the differences between these two methods, we measured the height and body weight and calculated body mass index (BMI). Finally we analyzed the factors correlated with these lengths by linear regression test. Results : The results showed that one cun obtained by the directional methods were significantly different from one cun by the proportional methods in forearm. It was demonstrated that the length acquired with the directional method was more correlated with body weight and body mass index, while the length obtained by the proportional method was more correlated with the height.

사회환경교육의 교육과정 표준화 모형 및 통합프로그램 개발연구

박석순,최돈형,성효현,허명,강명희,이숙임,남상준 이화여자대학교 환경문제연구소 1998 이화환경연구 Vol.2 No.-

This paper presents the development of standardized modules and an integrated program which will be used for social environmental education. The study objectives are to develop modules which are based on various environmental topics, to relate the regional environmental topics to the educational modules and program, and to include the demand of students in the modules and program. Literature and questionary surveys, as well as, module development method were utilized as research tools. The titles of developed modules are "The Green Plant Earth," "Unexpected Resources: Save Resources and Recycle", "Water as the Mother of All Lives", "The Blue Sky with Clean Air", and "Soil as the Home of All Living and Non-living Things". It is hoped that the developed modules and program are utilized as course materials and curriculum for social environmental education in various educational institutes. The study results will be materialized as multimedia in near futher.

Multiple myeloma 환자에서 Bortezomib치료 후 발생한 마비성 장폐색증 1례

김준영,이원식,손창학,박성길,진한영,박석주,조영완,정은욱,강명주,박정하,주영돈 인제대학교 2006 仁濟醫學 Vol.27 No.-

Bortezomib is the first clinically available proteasome inhibitor that is clinically effective in the treatment of multiple myeloma. A proteasome inhibitor acts through multiple mechanisms to arrest tumor growth, tumor spread, and angiogenesis. The main adverse effects of bortezomib are gastrointestinal symptoms, cytopenia, fatigue, and peripheral neuropathy. To date, severe paralytic ileus has not been reported as a toxic effect of bortezomib treatment in multiple myeloma. Bortezomib is a novel agent that has only been used clinically for 30 months, so a need exists to further evaluate its toxicity. We report a case of grade Ⅲ (NCI CTCAE v3.0) or grade Ⅳ(SWOG toxicity criteria) paralytic ileus in a 65-year-old man with relapsed multiple myeloma who underwent one cycle of single-agent bortezomib treatment scheduled in a 21-day cycle (1.3 mg/㎥ as a single i.v. bolus on days 1, 4, 8, and 11).

카르복시 폴리술폰 아이오노머의 기계적 성질

유지강,이용혁,김희석,김준섭,박기주,D. Nguyen,A. Eisenberg 조선대학교 생산기술연구소 1997 生産技術硏究 Vol.19 No.1

The dynamic mechanical properties of carboxylated polysulfone ionomers were studied by using DMTA. It was found that at 25 mol% of ions, the ionomer shows two glass transitions: one at ca. 200 ℃ and the other at ca. 235 ℃. It was also found that with increasing an ion content to ca. 37 mol%, a matrix T_(g) shifts to ca. 210 ℃, but a cluster T_(g) remains at the same temperature; however, the sizes of tan δ peaks for each transitions do not change with the ion content. This result means that even at those high ion contents, there is sufficient amount of a unclustered material in the samples, and the increase in the ion content does not increase the volume fraction of a cluster phase. In addition, the difference in two T_(g)s of this ionomer system was found to be ca. 40 ℃, which is smaller than that in poly(styrene-co-methacrylate) ionomer system. This finding implies that in the polysulfone ionomers weak ionic interactions in a multiplet resulting from steric hindrance due to the bulkiness of benzene ring to which the ionic group is attached and the tension on polymer chains surrounding the multiplet due to chain rigidity affect the size and stability of the multiplet significantly.

스타이렌 아이오노머의 기계적인 성질에 미치는 혼합 양이온의 영향

김희석,나연화,이용혁,이법원,유지강,박기주,김준섭 조선대학교 생산기술연구소 1998 生産技術硏究 Vol.20 No.1

The styrene-co-methacrylate ionomers neutralized with Na +, Ba2 +, and mixed cation of both were prepared. The dynamic mechanical properties of two cation-containing ionomers were measured and compared to those of the ionomers neutralized with only one type of cations. We found that in the ionomers with one type of cations the difference in the type of cation does not change the glass transition temperatures. It was found, however, that the cluster Tg of the mixed cation ionomer is lower than that of the single cation ionomers. Thus it was postulated that in the two-cation system the two cations in a solution have sufficient dynamics to form new type of multiplet containing both cations, which may be slightly bigger than that found in the single cation multiplet. The mechanical properties also imply that the strength of ion interactions in the multiplets of the two cation ionomer is thought to be weaker than that of the one cation ionomers. These two effects are believed to contribute the lower in the cluster glass transition of mixed cation system.

고온 초전도체의 국부�치 에너지에 따른 온도분포 해석

주민석,배준한,고태국,강연일 연세대학교 산업기술연구소 1994 논문집 Vol.26 No.1

The transient one-dimensional heat conduction equation in a high Tc bulk superconductor with an localized hot zone has been analyzed numerically. After the heater energy is introduced in a few miliseconds, the development of the normal zone and full quench time are calculated with respect to distance as a fuction of three parameters including : transport current, heater current, heating time. The analysis of this model is performed using finite difference method under modified adiabatic condition. The results of computer simulation are also presented in this paper. Full quench occurs when transport currents exceed 1.15[A] in given heater current and heating time. A temperature of superconductor rises abruptly in heating region when heater energy is increased. If heater energy is same, heating time is dominant factor for quench. Given heater energy, ohmic heat dissipation is dominant part for quench. But, heating energy is dominant part for quench than conduction. When heater energy is large, quench is not affected by transport currents.

Virus Associated Hemophagocytic Syndrome 3례

전동석,황종문,김준식,서은숙,강진무 啓明大學校 醫科大學 1990 계명의대학술지 Vol.9 No.3

Virus associated hemophagocytic syndrome is characterized by a benign generalized histiocytic proliferation with marked hemophagocytosis associated with systemic viral infectioin. The syndrome is a non-neoplastic, potentially reversible process and manifested fever, severe constitutional symptoms, hepatosplenomegaly, and lymphadenopathy, and characterized by laboratory findings of pancytopenia, hemophagocytosis and abnormal liver function test and coagulopathy. Immunosuppressive and cytotoxic therapy are contraindicated because of increasing risk of fatal outcome. The authors experienced three cases of virus associated hemophagocytic syndrome diagnosed by clinical features, bone marrow aspiration and lymph node biopsy. All cases had higt fever, hepatosplenomegaly and lymphoadenopathy and showed granulocytopenia in peripheral blood and phagocytized histiocytes in bone marrow. The brief review of the literatures was made.