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Jeong, Joojin,Cho, Sang-Yun,Lee, Wang-Hyu,Lee, Kui-jae,Ju, Ho-Jong The Korean Society of Plant Pathology 2015 Plant Pathology Journal Vol.31 No.3
The primary step for efficient control of viral diseases is the development of simple, rapid, and sensitive virus detection. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) has been used to detect viral RNA molecules because of its simplicity and high sensitivity for a number of viruses. RT-LAMP for the detection of Potato virus X (PVX) was developed and compared with conventional reverse transcription polymerase chain reaction (RT-PCR) to demonstrate its advantages over RT-PCR. RT-LAMP reactions were conducted with or without a set of loop primers since one out of six primers showed PVX specificity. Based on real-time monitoring, RT-LAMP detected PVX around 30 min, compared to 120 min for RT-PCR. By adding a fluorescent reagent during the reaction, the extra step of visualization by gel electrophoresis was not necessary. RT-LAMP was conducted using simple inexpensive instruments and a regular incubator to evaluate whether RNA could be amplified at a constant temperature instead of using an expensive thermal cycler. This study shows the potential of RT-LAMP for the diagnosis of viral diseases and PVX epidemiology because of its simplicity and rapidness compared to RT-PCR.
조승연,김주진,임정민,류동주,Cho, Seungyeoun,Kim, Joojin,Lim, Jeong-Min,Ryu, Dong-Joo 한국토지주택공사 토지주택연구원 2015 LHI journal of land, housing, and urban affairs Vol.6 No.4
본 연구는 "도시재생 활성화 및 지원에 관한 특별법(이하 도시재생특별법)"에 의한 도시재생사업에서 공공시행자의 역할을 모색하기 위하여 일본의 도시재생사업을 분석하여 국내에의 시사점을 도출하고자 하는 연구이다. '도시재생법'에서는 공공의 역할이 강조되고 있으나 도시재생활성화계획 수립 이후 사업시행단계에서의 공공의 역할에 대한 언급되지 않고 있어 향후 도시재생사업을 수행함에 있어서 일본 사례를 구체적으로 고찰해보는 것은 중요한 의의가 있는 것으로 판단된다. 구체적으로 일본 도시재생사업에서 공공시행자의 역할을 수행하고 있는 도시재생기구(UR)가 수행한 도쿄의 오오테마치 1-1지구, 시노노메 지구, 시부야역 가구, 오시아게 나리히라바시 4개 지구에 대한 심층 사례분석을 실시하였다. 사업수행체계에서 각 주체가 어떠한 역할을 담당하는지를 고찰해봄으로써 도시재생사업의 성공적인 수행을 위한 공공시행자의 역할을 제시하고자 하였다. This study is focused on the public developer's role of urban regeneration projects in Japan to derive implications for economy-based-urban regeneration programs by the Special Act on Activation and Support for Urban Regeneration (Urban Regeneration Special Act). 4 case studies; Otemachi 1-1, Shinonome, Shibuya Station and Osiage-Narihirabashi were delivered to review public sectors' roles. Central government, local government and public developer play key roles each step. Urban Regeneration Special Act doesn't set up public sector's role to deliver urban regeneration projects after establishment of master plan. These cases build the governance organization to deliver urban regeneration projects among diverse interest groups including private sectors and landowners. Especially, public developers play a key role as a coordinator and a developer throughout whole urban regeneration projects.