RIPL peptide-conjugated nanostructured lipid carriers for enhanced intracellular drug delivery to hepsin-expressing cancer cells

Lee, Sang Gon,Kim, Chang Hyun,Sung, Si Woo,Lee, Eun Seok,Goh, Min Su,Yoon, Ho Yub,Kang, Myung Joo,Lee, Sangkil,Choi, Young Wook Dove Medical Press 2018 International journal of nanomedicine Vol.13 No.-

<P><B>Background</B></P><P>To facilitate selective and enhanced drug delivery to hepsin (Hpn)-expressing cancer cells, RIPL peptide (IPLVVPLRRRRRRRRC, 16-mer)-conjugated nanostructured lipid carriers (RIPL-NLCs) were developed.</P><P><B>Methods</B></P><P>NLCs were prepared using a solvent emulsification-evaporation method and the RIPL peptide was conjugated to the maleimide-derivatized NLCs via the thiol-maleimide reaction. Employing a fluorescent probe (DiI), in vitro target-selective intracellular uptake behaviors were observed using fluorescence microscopy and flow cytometry. Separately, docetaxel (DTX) was encapsulated by pre-loading technique, then cytotoxicity and drug release were evaluated. In vivo antitumor efficacy was investigated in BALB/c nude mice with SKOV3 cell tumors after intratumoral injections of different DTX formulations at a dose equivalent to 10 mg/kg DTX.</P><P><B>Results</B></P><P>RIPL-NLCs showed positively charged nanodispersion, whereas NLCs were negatively charged. DTX was successfully encapsulated with an encapsulation efficiency and drug loading capacity of 95–98% and 44-46 µg/mg, respectively. DTX release was diffusion-controlled, revealing the best fit to the Higuchi equation. Cellular uptake of DiI-loaded RIPL-NLCs was 8.3- and 6.2-fold higher than that of DiI-loaded NLCs, in Hpn(+) SKOV3 and LNCaP cells, respectively. The translocation of RIPL-NLCs into SKOV3 cells was time-dependent with internalization within 1 h and distribution throughout the cytoplasm after 2 h. DTX-loaded RIPL-NLCs (DTX-RIPL-NLCs) revealed dose-dependent in vitro cytotoxicity, while drug-free formulations were non-cytotoxic. In SKOV3-bearing xenograft mouse model, DTX-RIPL-NLCs significantly inhibited tumor growth: the inhibition ratios of the DTX solution-treated and DTX-RIPL-NLC-treated groups were 61.4% and 91.2%, respectively, compared to those of the saline-treated group (control).</P><P><B>Conclusion</B></P><P>RIPL-NLCs are good candidates for Hpn-selective drug targeting with a high loading capacity of hydrophobic drug molecules.</P>

Does strength deficit correlate with shoulder function in patients with rotator cuff tears? Characteristics of massive tears

Oh, Joo Han,Yoon, Jong Pil,Kim, Dong Hyun,Chung, Seok Won,Kim, Joon Yub,Lee, Hyun-Joo,Il, Seo,Park, Kyeong Hyeon,Lee, Hoseok Elsevier 2019 Journal of shoulder and elbow surgery Vol.28 No.10

<P><B>Background</B></P> <P>The correlation between shoulder strength deficits and function in rotator cuff tears remains uncertain. This study aimed to determine the correlation between shoulder strength deficits and shoulder function evaluated by various clinical scoring systems.</P> <P><B>Methods</B></P> <P>A total of 262 patients (mean age, 59.67 years [standard deviation, 8.06 years]) who underwent full-thickness rotator cuff repair were included. Patients in group I (n = 188) had small to large rotator cuff tears, whereas those in group II (n = 74) had massive rotator cuff tears. Demographic factors, isokinetic test results, and shoulder function evaluated using various scoring systems were obtained. Correlation differences according to severity of the rotator cuff tear were evaluated.</P> <P><B>Results</B></P> <P>We found weak correlations between shoulder strength deficits (peak torque and total work) and clinical outcomes in patients with rotator cuff tears (<I>r</I> = –0.288). For patients in group I (nonmassive tears), we found a weaker correlation (<I>r</I> = –0.242) according to the tear pattern. However, shoulder strength deficits in group II patients (massive tears) were strongly correlated with American Shoulder and Elbow Surgeons (<I>r</I> = –0.598), Constant (<I>r</I> = –0.582), and Short Form 36 (<I>r</I> = –0.511) scores, especially regarding internal rotator strength deficits.</P> <P><B>Conclusions</B></P> <P>Shoulder strength deficits measured via isokinetic testing and shoulder function were weakly correlated in patients with rotator cuff tears. However, shoulder strength deficits in patients with massive tears considerably worsened shoulder function and systemic disability, but not regional disability. In particular, internal rotator strength deficits were strongly correlated with poor shoulder function.</P>

Echocardiographic left atrial volume index correlates with systolic blood pressure profile in ambulatory blood pressure monitoring (초)

( Hee Seung Lee ),( Woo Ri Park ),( Young Rak Choi ),( Young Kwang Shim ),( Ki Hyun Kim ),( Joo Hee Lee ),( Sang Min Kim ),( Sang Yub Lee ),( Jang Hwan Bae ),( Kyoung Kuk Hwang ),( Dong Woon Kim ),( M 대한내과학회 2010 대한내과학회 추계학술발표논문집 Vol.2010 No.-

원료의 배합 비율에 따른 펄프몰드 구조 및 흡수성 변화 연구

성용주(Yong Joo Sung),김동성(Dong Sung Kim),이주엽(Joo Yub Lee),백민석(Min Seok Baek),이지영(Ji Young Lee) 한국펄프·종이공학회 2018 펄프.종이기술 Vol.50 No.5

The effects of pulp types on the structure and the water absorption properties of pulp mold were investigated. The ONP (old newspaper recycled pulp), Sw-TMP (softwood thermomechanical pulp), Hw-BKP (hardwood bleached kraft pulp) were selected for this study. The pulp mold samples were prepared by using the laboratory wet pulp mold simulator. The pulp mold samples made with ONP showed lower bulkiness, lower Klemm water absorption property, lower maximum water contents and lower saturated water contents than those of the pulp mold made with Hw-BKP, which might be originated from the lower hydrophilicity and the higher ash contents of ONP. The addition of Sw-TMP increased the bulk and the water absorption properties of the pulp mold samples made with ONP. However, in case of the pulp mold samples made with Hw-BKP, the higher addition of Sw-TMP resulted in the lower Klemm water absorption and the lower saturated water contents. These results showed that the structural properties and the water absorption properties of pulp mold could be controlled by the raw materials depending on the fiber properties.

온천수의 아토피피부염 치료효과에 대한 예비임상연구

이준호 ( Joon Ho Lee ),진상윤 ( Sang Yun Jin ),최윤석 ( Yun Seok Choi ),권혁부 ( Hyok Bu Kwon ),이승호 ( Seung Ho Lee ),이중엽 ( Joong Yub Lee ),박병주 ( Byung Joo Park ),이애영 ( Ai Young Lee ) 대한천식알레르기학회 2011 천식 및 알레르기 Vol.31 No.2

Background: Spa has been applied for the alleviation of atopic dermatitis (AD), but there have been few studies on the efficacy and safety of hot spring sea water therapy. Objective: This study aimed to clarify the conditions which have adequate efficacy and safety of hot spring sea water therapy for the treatment of AD. Method: Twenty-eight patients with AD who had mild to moderate symptoms were enrolled and divided into 14 groups according to bathing frequencies, temperatures, durations, and kinds of water. Bathing frequency was 2, 3 or 7 times a week. Temperature was 37˚C or 40˚C, and duration was 15 or 30 minutes. Twelve groups took a bath in hot spring sea water obtained from an island in Korea, 1 group took a bath in salt water and 1 took a bath in tap water. We assessed o-SCORAD index at baseline and every week for 4 weeks. In addition, colony counts of S. aureus were evaluated at baseline and at the last visit. Result: The 3 times-a-week/37˚C/15 minute and twice-a-week/37˚C/30 minute groups showed the largest and continuous decline in o-SCORAD index. The index was also slightly decreased in the salt and tap water groups. The colony counts of S. aureus did not show significant changes after bathing. Conclusion: This pilot study shows the possibility that hot spring sea water therapy might be an efficacious and safe treatment option for treating AD. However, the precise mechanism should be elucidated through further studies. (Korean J Asthma Allergy Clin Immunol 2011;31:124-130)

원발성 간외 담관암의 임상적 고찰 : 예후 인자 분석을 중심으로

김성호,박완,이정희,김명환,이성구,민영일,주광로,방성조,주연호,명승재,공경엽,김해경,이철룡 대한소화기학회 1999 대한소화기학회지 Vol.33 No.1

Background/Aims: The prognosis of extrahepatic bile duct carcinoma is very poor. The value of the gross morphology and location of the tumor in the bile duct as prognostic factors have been contro versial. Thus, we conducted a retrospective analysis of the prognostic factors. Methods: During the period between June, 1989 and December, 1996, 193 cases of extrahepatic bile duct carcinomas were selected and 108 cases of them were curatively resected. We collected data at the point of August 1997. Results: The mean age was 61.9 (17-87) years and the male to temale ratio was 2.5:1. Th frequency of tumor according to the location was 32.1% in the upper part, 31.6% in the middle par and 5.3% in diffuse type. The overall 1,2,3, and 5-year survival rate was 49.9%, 32.2%, 23.4%, and 17.8%, respectively. In the patients with curative surgery (n=108), the 1-, 2-, 3-, and 5- year surviva rate was 70.0%, 48.0%, 37.1% and 27.2%, respectively. In the patients without carative surgery (n=85), the 1-, 2-, 3-, and 5-year survival rate was 24.0%, 12.5%, 7.1%, and 3.0%, respectively. The survival rates of stage I were significantly better than those of stage II, stage III and stage IV-A. Th survival rates of well differentiated histology were significantly better than those of moderate or poo differentiation. Conclusions: The stage, histologic differentiation and curative resectability of the extrahepatic bile duct carcinoma were significant prognostic factors. However, the gross morphology and location of the tumor had no prognostic significance. (Kor J Gastroenterol 1999;33:114 - 123)

양성 갑상선 결절에서의 무수알코올치료

김홍규,김진엽,이은주,안일민,이미헌,이성진,이호규 대한내분비학회 1998 Endocrinology and metabolism Vol.13 No.3

Background: Percutaneous ethanol injection(PEI) performed in guidance of ultrasonography has been used in cases of thyroid cyst and autonomous functiong thyroid nodule(AFTN). We performed this study to determine the feasibility of PEI on the various type of benign cold nodules(solid, pure cyst, complex cyst) and AFTN. Method: Ninety patients(age 41+12 years; 83 women and 7 men) with hot and cold nodule were included in this study. All cases were subjected to FNAB, and sono-guided in cases of complex cyst, at least twice with results of colloid nodule. T4 suppression treatment was done for cold solid nodules for at least 6 months and cases which had partial response(50% or more volume reduction but no further volume decrease on T4 suppression) were included in this study. After PEI, we classified AFTN into three different response groups; complete response (normali- zation of TFT and thyroid scan finding), partial response(normalized freeT4 but suppressed TSH or persistently suppressed scan), or failure group. Each subtypes(solid, pure cyst, complex cyst) of cold nodules were also classified into three groups in accordance with volume reduction; complete response(above 90% of volume reduction), partial response(50-90%), and failure(below 50% or increase in size) group. Results: Overall responses rate of PEI for benign thyroid nodule were complete; 70(78%), partial; 18(20%), and failure; 2(2%). In 27 cold solid nodules, complete response was observed in 23(85%) and partial response in 4(15%). In 11 pure cysts, complete response was observed in 7(64%), partial response in 3(27%), and 1 case(9%) of failure was lost after 1 trial of PEI. In 45 cases of complex cyst, complete response was observed in 36(80%), partial response in 8 (18%), and 1 case(2%) of failure was subjected to operation with pathology report of Hiirthle cell adenoma. In 7 cases of AFTN, complete response was observed in 4(57%) and partial response in 3(43%). We observed complications during PEI therapy such as transient neck pain(n=10), transient unilateral vocal cord palsy(n=l), and intracavitary hemorrhage(n=2), transient hypotension during ethanol injection(n 1). Conclusion: Our data suggest that efficacy of PEI(57%) in AFTN is inferior to conventional therapies like surgery and radioiodine, but still can be an alternative therapeutic modality in selected cases. In cold nodules, especially of solid type and complex cyst, PEI may have feasibility as a therapeutic modality in restricted cases. Further studies of prolonged follow-up for the possibility of neglecting occult malignancy are warranted(J Kor Soc Endocrinol 13:373-383, 1998).

小兒의 先天性 巨大水腎症 1例

李炳燁,金周龍,尹德鎭,이진무,裵寶石 대한소아과학회 1970 小兒科 Vol.13 No.2

Degradation of Phenol with Fenton-like Treatment by Using Heterogeneous Catalyst (Modified Iron Oxide) and Hydrogen Peroxide

Lee, Si-hoon,Oh, Joo-yub,Park, Yoon-chang Korean Chemical Society 2006 Bulletin of the Korean Chemical Society Vol.27 No.4

Goethite, hematite, magnetite and synthesized iron oxide are used as catalysts for Fenton-type oxidation of phenol. The synthesized iron oxides were characterized by X-ray diffraction (XRD), BET, X-ray photoelectron spectroscopy (XPS), and electron paramagnetic resonance (EPR). The catalytic activity of these materials is classified according to the observed rate of phenol oxidation. The effectiveness of the catalysts followed the sequence: ferrous ion > synthesized iron oxide >> magnetite hematite > goethite. According to these results, the most effective iron oxide catalyst had the structure similar to natural hematite. The surface oxidation state of the catalyst was between magnetite and hematite (+2.5 ~ +3.0). Phenol degraded completely in 40 min at neutral pH (pH = 7). Soluble ferric and ferrous ions were not detected in the filtrate from Fenton reaction solution by AAS. The formation of hydroxyl radicals was confirmed by EPR.

A Note On Prolate Spheroidal Wave Functions And Prolate Function Based Numerical Inversion Methods

( Eun Joo Kim ),( Jung Yub Lee ) 한국산업응용수학회(구 한국산업정보응용수학회) 2008 한국산업정보응용수학회 Vol.12 No.1

Polynomials are one of most important and widely used numerical tools in dealing with a smooth function on a bounded domain and trigonometric functions work for smooth periodic functions. However, they are not the best choice if a function has a bounded support in space and in frequency domain. The Prolate Spheroidal wave function (PSWF) of order zero has been known as a best candidate as a basis for band-limited functions. In this paper, we review some basic properties of PSWFs defined as eigenfunctions of bounded Fourier transformation. We also propose numerical inversion schemes based on PSWF and present some numerical examples to show their feasibilities as signal processing tools.