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RISS 인기검색어
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- 저자 : Jonghoon Yang (검색결과 79 건)
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Yang, Kiwoung,Robin, Arif Hasan Khan,Yi, Go-Eun,Lee, Jonghoon,Chung, Mi-Young,Yang, Tae-Jin,Nou, Ill-Sup MDPI 2015 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.16 No.12
<P>Ribosomal DNA (rDNA) of plants is present in high copy number and shows variation between and within species in the length of the intergenic spacer (IGS). The 45S rDNA of flowering plants includes the 5.8S, 18S and 25S rDNA genes, the internal transcribed spacer (ITS1 and ITS2), and the intergenic spacer 45S-IGS (25S-18S). This study identified six different types of 45S-IGS, A to F, which at 363 bp, 1121 bp, 1717 bp, 1969 bp, 2036 bp and 2111 bp in length, respectively, were much shorter than the reported reference IGS sequences in <I>B. oleracea</I> var. <I>alboglabra</I>. The shortest two IGS types, A and B, lacked the transcription initiation site, non-transcribed spacer, and external transcribed spacer. Functional behavior of those two IGS types in relation to rRNA synthesis is a subject of further investigation. The other four IGSs had subtle variations in the transcription termination site, guanine-cytosine (GC) content, and number of tandem repeats, but the external transcribed spacers of these four IGSs were quite similar in length. The 45S IGSs were found to follow Mendelian inheritance in a population of 15 F<SUB>1</SUB>s and their 30 inbred parental lines, which suggests that these sequences could be useful for development of new breeding tools. In addition, this study represents the first report of intra-specific (within subspecies) variation of the 45S IGS in <I>B. oleracea.</I></P>
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Jonghoon Lee,Junki Lee,Jee Young Park,Tae-Jin Yang 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
Chloroplasts are plant-specific organelles, which have their own genome. Most of the plant chloroplast genomes (CP genome) are highly conserved in terms of its gene contents and genome structures, and they exist in cells with abundant copy numbers. Because of numerous copy numbers, the complete chloroplast sequence assembly pipeline with small amount of whole genome resequencing data, produced by NGS technique, was established in our laboratory. From 14 accessions of cabbage (Brassica oleracea L.) resequencing data produced by Illumina Hi-seq 2000, CP genomes were assembled and compared to each other. 18 sequence variance regions were detected, and 6 HRM(High Resolution Melting curves) markers were developed. Approximately 1 Gb of whole genome sequencing data of 10 Brassica rapa and 2 Brassica napus were also obtained from Institute of Vegetables and Flowers, Chinese Academy of Agricultural Science. With these resequencing data, all CP genomes from these accessions were assembled. Total 27 complete CP genomes of B.oleracea, B.rapa, B.napus, and brassico-raphanus which is a novel allotetraploid species between B.rapa and Raphanus sativus, were compared in sequence level. Phylogenetic analysis based on the comparison revealed that B.rapa could be the maternal species when rapeseeds and brassico-raphanus became allotetraploid species. Additionally, CP genome of B.napus cv.M083 is closer to B.rapa accessions than the other B.napus accessions, thus B.napus could have several different origins.
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Kiwoung Yang,Nasar Uddin Ahmed,Jonghoon Lee,Junki Lee,Ill-Sup Nou,Tae-Jin Yang 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
Mitochondria are essential organelles of eukaryotic cells and plant cells contain varying numbers of mitochondrial genome sequences. Sizes and shapes of mitochondria differ within a tissue or in the same cells. Previously sequenced complete mitochondrial genome (NC_016118) of Brassica oleracea size was 360,271 bp, where segmental duplication (repeat block) was 141,800 bp. In this study, we resequenced this whole mitochondrial genome by using WGS (whole genome sequencing) and assembled organelles genome method (unpublished). Newly sequenced mitochondrial genome length was 219,975 bp and circle form. A new sequence segment of approximately 4,800 bp was obtained compared to the previous genome sequence without any large repeat block. Newly obtained mitochondria genome sequence was compared with recently reported mitochondria genome sequences of various species (B. oleracea, B. juncea, B. rapa, B. napus and B. carinata) and subspecies (cabbage, cauliflower, brussels sprouts, kohlrabi, broccoli and kale) by PCR using primers specifying different region of genome sequences. PCR analysis results have also confirmed the variation between previous and newly sequenced mitochondrial genome circles form. Thus, the results suggest new B. oleracea mitotype, including evolutionary events such as inheritance, rearrangement, genome compaction, and diversity
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인공지능으로부터 설명된 자아 〈eXplainable Human〉
양종훈(Jonghoon Yang),석정현(Jeonghyun Seok),김나은(Naeun Kim),탁나영(Nayeong Tak),한혜지(Hyeji Han),주다영(Da Young Ju) 한국HCI학회 2023 한국HCI학회 학술대회 Vol.2023 No.2
본 작품은 관객과 인공지능 간의 대화를 통해 자신의 자아에 대해 설명받는 인터랙티브 미디어아트이다. 최근 인공지능 기술이 발전함에 따라 미디어아트에서도 인공지능을 활용하는 시도가 증가하고 있다[1]. 이러한 흐름 속 본 작품은 흔히 떠오르는 정교한 인공지능의 모습과 달리 단편적 데이터로 이루어져 명확한 한계를 의도적으로 드러내는 인공지능을 제작하여 그 신뢰성에 대한 의문을 던지는 것으로 시작된다. 또한 이런 인공지능의 한계를 작품의 메시지로 활용하여 단순하게 설명되지 않는 자아의 입체성과 모호성을 표현하였다. 특히 인공지능의 한 분야인 XAI(eXplainable AI)를 모티브로 삼아 이러한 자아의 설명 가능성을 다루고자 하였다. 작품 속 질문과 답변을 주고받는 과정에서 인공지능 대형언어모델인 GPT-3 가 활용되었으며 이는 자아에 대한 작품의 마지막 답변을 내놓는데 반영된다. 작품에 참여한 관객은 인공지능과 대화하며 자신의 자아에 대해 설명받고 이에 대한 일치와 충돌을 경험하게 된다. 그리고 이를 통해 자아는 설명 가능한가에 대한 질문을 관객에게 던진다.
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