소 수정란의 공배양 및 동결이 체외발생에 미치는 영향에 관한 연구

이종진,이명헌,남윤이,이만휘,이봉구,김상근 충남대학교 수의과대학 동물의과학연구소 1996 動物醫科學硏究誌 Vol.4 No.-

The studies on the carried out to investigate the effects of co-culture with cumulus cells and oviduct epithelial cells on the in vitro fertilization and cleavage rate of bovine follicular oocytes, and to determine the optimum thawing temperature and equilibration time of frozen bovine embryos and on survival rate and in vitro developmental rate of bovine embryos. The ovaries were obtained from slaughtered Korean native cows. The follicular oocytes were cultured in TCM-199 medium containing 10 IU/㎖의 PMSG(Sigma, USA), 10 IU/㎖의 hCG, 1 ㎍/㎖의 β-estradiol(Sigma, USA) and 10% FCS(Sigma, USA) for 24-48 hrs in incubator with 5% CO_2 in air at 38.5℃ and then matured oocytes were again cultured for 12-18 hrs with motile capacitated sperm by preincubation of heparin. The bovine embryos following dehydration by cryoprotective agents and a various concentrations of sucrose were directly plunged into liquid nitrogen and thawed in 30℃ water. The results are summarized as followes : 1. The in vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured with cumulus cells in TCM-199 media were 75.0%-76.8% and 17.3%-27.6%, respectively. And in-vitro fertilization rates of cumulus-enclosed oocytes(55.4%) were significantly(p<0.05) higher than cumulus-denuded oocytes(23.1%). 2. The in vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured with 1 ×10^4 cells/㎖, 1 ×10^6 cells/㎖, 1 ×10^8 cells/㎖ and 1 ×10^15 cells/㎖ oviduct epithelial cells in TCM-199 media were 74.5%-77.8% and 15.7%-21.2%, respectively. 3. The in-vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured in TCM-199 media containing PMSG, hCG, PMSG + hCG, PMSG + β-estradiol, hCG + β-estradiol 0 to 40 hrs after insemination were 74.0%-77.4% and 18.9% - 23.1, respectively. 4. The survival rates of bovine embryos thawed after rapid freezing in the media containing various kinds of cryoprotective agents added 0.25M and 0.50M sucrose were 14.7% - 35.1% and 17.6% - 31.6%, respectively. The survival rates of bovine embryos thawed after rapid freezing in the freezing media containing a various concentration of sucrose added 1.5M and 2.0M glycerol, 1.5M and 2.0M DMSO, and 1.5M and 2.0M propanediol were 23.5% -31.4% and 20.6% - 34.1%, respectively. 5. The temperature thawed at 30℃ after rapid freezing of bovine embryos resulted in a significantly higher embryos survival rate than did at 20℃ and 35℃. 6. The equilibration time on the survival rates of bovine embryos was attained after short period of time(2.5-5 min.) in the freezing medium higher than ling period of time(10-20min.).

양단힌지 비대칭 포물선아치의 자유진동에 관한 연구

이병구,남궁문,김종만 圓光大學校 1986 論文集 Vol.20 No.2

양단힌지 비대칭 포물선아치의 자유진동을 지배하는 미분방정식가 경계조건을 D'Alembert원리와 평형방정식을 이용하여 유도하였다. 유도된 미분방정식에는 회전관성의 영향을 고려하였다. 위의 미분방벙식을 수치적분하기 위하여 Runge-Kutta Method가 이용되었으며, 고유 진동수 매개변수의 값F를 찾기 위하여 시행착오적 고유치문제가 이용되었다. 상세연구로는 양단힌지 포물선아치의 최저차의 3개의 고유진동수를 해석하였다. 회전관성이 고유진동수에 미치는 영향은 무시할 정도로 작았다. 또한 수치해석의 결과로 고유진동수-아치 지간길이의 관계를 그림에 나타내었다. The governing differential equations and the boundary conditions for the free vibration of hinged ended unsymmetric parabolic arch are derived on the basis of the equilibrium equations and the D'Alembert principle. The effect of rotary inertia as well as extentional and flexural deformations is considered in governing differential equations. A trial eigen value method is used for determining the natural frequencies. The Runge-Kutta method is used in this method to perform the integration of the differential equations. The detailed studies are made of the lowest three vibration frequencies for the hinged ended unsymmetric parabolic arches. The effect of the rotary inertia is analyzed and it's numerical data are presented in table. And as the numerical result the frequency versus the span length of arch is presented in figure.

만성 간질환에서 혈청 Type Ⅳ Collagen 측정의 의의

이헌영,김성걸,정현용,구선회,박종우,강대영 충남대학교 의과대학 지역사회의학연구소 1994 충남의대잡지 Vol.21 No.2

To evaluate the clinical significance of serum type N collagen levels in patients with various liver diseases, serum type IV collagen levels were measured by one-step sandwich enzyme immunoassay using monoclonal antibodies in 80 patients from December 1993 to December 1994 at Chung Nam National University Hospital. The results were as follows : 1. Mean age of patients was 45 years. Men was 2.1 times more than women. 2. Compared to 15 normal controls(159.9±34.0 ng/ml), serum type IV collagens were significantly elevated in patients with chronic hepatitis (254.5±158.0 ng/ml), liver cirrhosis(281.0±195.7 ng/ml) and hepatocellular carcinoma(420.5±241.4 ng/ml) (p<0.005). 3. Serum type IV collagen levels were increased without significance in patients with alcholic liver cirrhosis(331.6±244.5 ng/ml) compared to non-alcholic cirrhosis(230.4±117.8 ng/ml. p>0.05). 4. The serum levels of type IV collagen were not correlated with the levels of alanine amino-trnasferase(ALT), aspartate aminotransferase(AST) and alkaline phosphatase(ALP). The results suggest that the serum type IV collagen levels correlate well with the degree of progression of chronic liver diseases. Therefore the measurement of serum type IV collagen is relatively simple and useful method to reflect the progression of chronic liver diseases.

한약재가 Aspergillus parasiticus R-716의 生育과 aflatoxin 生成에 미치는 影響

구성회,이용욱,정덕화,김종규 한국식품위생학회 1988 식품위생학회지 Vol.3 No.2

한의원에서 오래 前부터 사용되어 오고 있는 몇 종의 한약재를 구입하여 이들을 chloroform으로 추출하여 추출물을 조제한 다음 공시균(Aspergillus parasiticus R-716)의 生育과 aflatoxin 生成에 미치는 影響을 조사한 결과 다음과 같은 結論을 얻었다. 1. 목단 추출물 첨가군에서 가장 현저하게 공시균의 生育이 저해되었고 그외의 첨가군에서도 강황, 봉출, 향부자, 백작액, 백출의 順으로 저해되었다. 2. Aflatoxin 生成은 백출과 강황 추출물 첨가군에서만 저해되었고, 나머지 첨가군에서는 오히려 증가되거나 효과가 적었다. 3. 백출 추출물 0.2ml첨가시 3일째부터 菌體 生成을 시작하여 9일째에 0.953g/30ml이었고, aflatoxin 함량은 792㎍으로서 대조군에 비해 약 50%가 감소되었다. 菌體 g當 aflatoxin 함량은 992㎍으로서 대조군의 1,467㎍에 비해 역시 크게 감소되었으며 NADPH oxidase 活性은 대조군보다 높아 백출 추출물이 공시균의 生育과 aflatoxin 生成에 저해 효과가 있는 것으로 나타났다. The possible effects of some oriental herbs, which have been used to treat cancerlike disease in Korea, on growth and aflatoxin production by Aspergillus parasiticus R-716 were investigated. Zedoaria rhizoma, Curcuma longa, Cyperus rotundus, Angelica gigas, Paeonia albiflora, Paeonia mountan, Atractylis ovata, and Pulsatilla koreana were extracted with chloroform. Among them the extract of Paeonia mountan was remarkably effective on the growth inhibition, and Curcuma longa, Zedoaria rhizoma, Cyperus rotundus, Paeonia albiflora, Atractylis ovata also inhibited the growth. The extract of Atractylis ovata and Curcuma longa, also inhibited the aflatoxin production but the others showed no effect at all or sometimes stimulated effect. With the addition of 0.2ml extract of Atractylis ovata in 30ml SLS medium, the growth was delayed for about 2 days, and after 9 days, mycelium weight was 0.953g, and total aflatoxin was reduced 50%(792㎍)of that produced in the control(1547㎍). Aflatoxin per mycelium weight was decreased 32%(992㎍)of that produced in the control(1467㎍), but NADPH oxidase was higher as compared to the control. The extract of Atractylis ovata appeared to have a inhibitory effect on the growth and aflatoxin production of Aspergillus parasiticus R-716.

인삼 사포닌의 흰쥐 취효소 분비에 대한 devazepide의 억제작용

이상호,이범구,이선미,박종대,조태순 성균관대학교 약학연구소 1999 成均藥硏論文集 Vol.11 No.-

Abstract-Recent studies have suggested that Panax ginseng saponins may stimulate pancreaticobiliary secretion. However, the precise mechanisms underlying the alterations in pancreaticobiliary function associated with ginseng saponins remain uncertain. We studied the effects of ginseng saponins and devazepide, cholecystokinin receptor antagonist, on pancreaticobiliary secretion in male Sprague-Dawley rats. The saponins tested were crude saponin (TS) and panaxatriol saponin (PTS). After single or two weeks administration of saponins, pancreaticobiliary juice of rats was collected for 8hrs. Single administration of TS and PTS did not change the volume of pancreaticobiliary juice compared with control group. In contrast, the pretreatment of devazepide significantly increased the volume of pancreaticobiliary juice. The amylase activity was significantly increased by acute TS treatment, but this increase was inhibited by devazepide pretreatment. In animals with two weeks administration of TS and PTS, the volume of pancreaticobiliary juice was not increased as compared to the control group. However, the volume of pancreaticobiliary juice was significantly increased by devazepide treatment. The amylase activity was significantly increased by two weeks administration TS and PTS, respectively. This increase was inhibited by devazepide treatment. Our findings suggest that ginseng saponins, especially panaxatriol, increase the amylase activity in pancreaticobiliary juice, and this is, in part, caused by release of endogenous cholecystokinin.

이식 탈회골의 골화에 대한 실험적 연구

구순상,이종원,이택호,신태선 大韓成形外科學會 1984 Archives of Plastic Surgery Vol.11 No.2

The most effective osseous graft material is the fresh autogenous bone in clinical practice and experimental studies. However, there are some problems in use of fresh autogenous bone graft, such as donor-site morbidity and the limitation in amount of available autogenous bone. Since the introduction of decalcified bone as grafting material by Senn(1889), decalcified bone has been studied as substitute for autogenous bone. The implantation of decalcified bone segment or powder in soft tissue has been established as a laboratory model for studying bone induction. Bone powders (particle size: 74∼420㎛) of rat and ox were decalcified with 0.5M HCI and were implanted i rat's abdomen subcutaneously. Thereafter, periodic observation was made histologically up to 30 days. Comparative studies were carried out to determine differences in histological sequence of induced osteogenesis and quantity of new bone. The results were as follows; 1. Implantation of decalcified bone powders of rat and ox to subcutaneous site in rats resulted in new bone formation by an enchondral sequence and there were no differences in histological change periodically. 2. There were no differences in quantity of new bone.

강의 현대화 추진 및 활용방안 연구

구경완,이근복,장종국,김재홍,김영환,최제영,이승훈 永同大學校 1997 硏究論叢 Vol.3 No.1

본 연구는 각종 첨단 기자재를 활용한 강의 현대화 방안을 모색함으로서 공학교육의 효율성을 도모하고 학생들의 학습동기를 유발하여 창조적 능력을 갖츤 전문기술 인력을 양성하는 데 도움을 주고자 하는 것이다. 이를 위하여 현행 공학교육의 현황과 문제점을 분석하고 강의 현대화 사례를 조사하여 본 연구의 기초자료로 삼고자 하였으며, 학부·전공별 강의 현대화 추진 실태분석 및 개선방안을 모색한 후 이상의 연구결과를 종합하여 향후의 강의 형대화 추진방향을 걸정하였다. This study aims to help bring up the well-skilled and creative engineers by searching the modernization of education using various high technology facilities. For this, we analyzed the general condition and problems of current engineering education and surveyed the case study of the modernization of education as basic study. After analyzing the existing situation of promotion searching the proper improvement methods of it, we proposed the new direction of the modernization of education from now on.

BGA 솔더 볼 로딩장치의 최적설계

이준환,이종원,구홍모 중앙대학교 생산공학연구소 1999 생산공학연구소 논문집 Vol.8 No.2

최근의 정보화 장비와 멀티미디어의 소형화 추세는 생산 설비중 반도체 패키지 기술레 힘입은 바 크다. BGA(Ball Grid Array types of package)와 CPS(Chip Scale Packages)는 QFP(Quad Flat Packages)를 대체하는 기술로 인정되고 있다. 볼을 탑재하는 장치는 기존에 진공 - 흡입장치가 많이 사용되어 왔다. 시소 타입 로더에서는 플래이트의 각도와 카트리지의 속도가 중요한 요소로 간주된다. 본 연구에서는 이에 대한 최적 속도와 각도를 제안하였다. With the trends of the recent micro-miniaturization of mutimedia and information equipment, electronic components packaging technology is playing a key role in production technology. BGA(Ball Grid Array thpes of package) and CSP(Chip Scale Packages) are replacing QFT(Quad Flat Packages). Vacuum-suction type loader is a popular loading device. For the seesaw type loader, the velocity fo cartridge and the angle of plate are very important elements. In this study, the optimal values of the velocity and the angle are proposed for the seesaw type loader.

성인 급성 신부전의 원인 및 예후인자 분석

구영선,장윤경,양종오,강민규,황평주,김종학,나기량,이강욱,신영태 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.2

Acute renal failure is a frequent complication in hospitalized patients and is strongly related to the mortality. The Clinical outcome and prognostic factors of acute renal failure(ARF) have been analyzed by many authors. The present study describes the etiologic and clinical aspects, as well as other factors related to mortality. All the patients suffering from acute renal failure admitted during the period of January 1993 - August 1998 were included in the study. The average age of the patients was 52±17 years and mortality rate was 24%. The causes of acute renal failure were hemorrhagic fever with renal syndrome(HFRS), sepsis, renal hypoperfusion, urinary tract obstruction, acute tubular necrosis, etc. The etiology of ARF was a significant prognostic factor on mortality in ARF. Other significant prognostic factors were oliguria, organ failure, use of vasoconstrictors, hypotension, serum bicarbonate, premorbid conditions, sepsis, neurologic complications, gastrointestinal bleeding. On the other hands, operation, sex, anemia, thrombocytopenia, hypoalbuminemia, BUN, serum creatinine, and hyperkalemia were not significant factors for the mortality. We conclude that major prognostic factors of acute renal failure arc premorbid conditions, sepsis and multiorgan failure, and they are responsible for persistent high mortality of acute renal failure despite of advances of medical care.

섬유상 담체 종류에 따른 생물학적 오수처리 비교연구

이수구,임병란,한종옥 서울産業大學校 1994 논문집 Vol.40 No.1

This study was performed to investigate the treatability of sewage from department store by attached growth biological treatment method using several fibrous media such as Supermodule made of polyvinylidine chloride and acry fiber(FBC), ringlace made of polyvinylidine chloride(HBC) and polypropylene(PP). The pilot plant was set up in the field of K department store and four consecutive aerated reactors were packed with each media and maintained in the condition of hydraulic retention time 12 hours. Experimental results showed that the effluent BOD concentration was stable even though the influent concentration was largely fluctuated in the range of 300~900㎎/L. BOD removal efficiencies in HBC, PP and FBC systems were 90%, 87% and 94%, respectively. The influent BOD was mainly removed in the first, second and third reactors and the removal rate in the fourth reactor was the lowest due to the low concentration and low influent load. The concentration of biomass attached to fibrous media was the highest in the FBC system compared with HBC and PP systems. Total concentration of volatile solids in the FBC system was 5,900 ㎎/L which was high compared with the other media systems. The production ratios of waste sludge were 9.2, 8.45 and 7.3 gDS/㎥ of influent sewage in HBC, PP and FBC systems, respectively.