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      • Current Status of Industrial Waste Generation and Symbiosis Network in Gyeongsangbuk-do, South Korea

        ( Jong-hun Park ),( In-gyung Jung ),( Jae-gun Seo ),( Sang-hyoun Kim ) 한국폐기물자원순환학회(구 한국폐기물학회) 2015 한국폐기물자원순환학회 춘계학술발표논문집 Vol.2015 No.-

        This work aimed to analyze current status of by-products generation and industrial symbiosis network in Gyeongsangbuk-do, South Korea. Manufacturing sector of the province mainly consists of electronics, machinery, steel, metal-working industries. Gumi and Pohang have the 1st and 2nd largest worker population in the manufacturing sector. 21,950.7 ton/d of waste is generated from the industries in 2011, of which 82.2% is recycled. The industrial waste, which is not recycled, is mainly composed of sludge cake from domestic wastewater treatment plants, waste plastic, incinerator ash, and slag. Pohang is the largest generating city of both total and the non-recycled industrial waste in the province, and most of the waste is from Pohang steel industrial park. Symbiosis map in Pohang steel industrial park shows that most of the current material symbiosis in the industrial park is for waste refractories and slag.

      • Current Status of Industrial Waste Generation and Symbiosis Network in Gyeongsangbuk-do, South Korea

        Jong-Hun Park,In-Gyung Jung,Jae-Gun Seo,Sang-Hyoun Kim 한국폐기물자원순환학회 2015 한국폐기물자원순환학회 학술대회 Vol.2015 No.05

        This work aimed to analyze current status of by-products generation and industrial symbiosis network in Gyeongsangbuk-do, South Korea. Manufacturing sector of the province mainly consists of electronics, machinery, steel, metal-working industries. Gumi and Pohang have the 1st and 2nd largest worker population in the manufacturing sector. 21,950.7 ton/d of waste is generated from the industries in 2011, of which 82.2% is recycled. The industrial waste, which is not recycled, is mainly composed of sludge cake from domestic wastewater treatment plants, waste plastic, incinerator ash, and slag. Pohang is the largest generating city of both total and the non-recycled industrial waste in the province, and most of the waste is from Pohang steel industrial park. Symbiosis map in Pohang steel industrial park shows that most of the current material symbiosis in the industrial park is for waste refractories and slag.

      • SCOPUSKCI등재

        김치유래 젖산균의 균체지방산 분석을 이용한 분류학적 연구

        이정숙,정민철,김우식,이근철,김홍중,박찬선,이헌주,주윤정,이근종,안종석,박완,박용하,민태익 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.2

        표준균주를 포함한 230여개의 김치유래 젖산균에 대한 균체지방산(FAMEs)을 분석하였다. FAMEs profiles는 Euclidian Distance 17.5에 의해 7개의 Major Cluster와 1개의 Single Cluster로 나뉘어졌다. 이중 A, B, C 및 Cluster는 Leuconostoc속으로 분석되어졌고, F는 Lactobacillus속으로 분석되어졌다. 그리고 E와 G cluster는 두개의 Genus가 혼재되어 나타났으며 보충적인 연구가 필요하다. 앞으로 김치유래 젖산균의 균체지방산 분석결과를 기반으로 한 데이타베이스에 95가지 탄소원을 이용하는 수치분류학적 접근방법 및 Pyrolysis Mass Spectrometry 등의 화학적 분석 방법과 분자친화적 연구를 통한 종합적 분류정보 체계가 갖추어지면 젖산균의 신속, 정확한 동정 및 연구에 활발히 이용되어질 것이다. Two hundreds and thirty lactic acid bacteria, mostly isolated from Kimchi, including type strains were sued for analysis of cellular fatty acids. The 230 test strains were recoverd in 7 major and 1 single clusters defined a Euclidian distance of 17.5. These aggregate taxa were equivalent to the genus Leuconostoc (aggregate group A, B, C and D), and the genera Leuconostoc and Lactobacillus (aggregate group G). It is concluded as evident that FAMEs (Fatty Acid Methyl Esters) profile of cell can be used as a criterion in classification of lactic acid bacterial from kimchi. Additional comparative taxonomic studies need to be carried out on well chosen representative strains to determine the most appropriate methods of value.

      • SCOPUSKCI등재

        전기장 인가에 따른 DNA 신장에 관한 AFM 연구

        박진영,한성웅,손세영,송성훈,김종민,Ohtani, Toshio,Muramatsu, Hiroshi,장상목 한국화학공학회 2002 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.40 No.1

        본 연구에서는 마이카 표면상에서 전기장에 의한 극히 낮은 농도의 DNA 신장에 대한 효율적인 방법론을 제시하였다. 전기장에 의한 DNA 신장을 AFM형상으로 분석한 결과, DNA 농도범위가 약 57×10 exp(-3)ng/mL-57×10 exp(-6)ng/mL일 때 매우 잘 적용되며, 신장된 DNA는 두 전극 중앙에 존재하였다. 또한, 일반 마이카, Mg^2+ 처리된 마이카, 그리고 AP-마이카 표면들에 대한 신장 효율의 차를 기술하였다. 동일한 실험조건 하에서 AP-마이카의 표면이 가장 좋은 효율의 DNA 신장 결과를 나타내었다. An effective method of DNA stretching using electric field on mica surface was proposed for extremely low solution concentration of DNA. DNA stretching based on electric field was observed by AFM and well applied on the concentration range from 57×10 exp(-3)ng/mL to 57×10 exp(-6)ng/mL. Stretched DNA existed in the middle of two electrodes. The difference in stretching efficiency for the different surfaces of bare mica, Mg^2+ soaked mica and AP-mica was discussed. The best performance of stretching was found from the surface of AP-mica under the same experimental condition.

      • 維新벼에 나타난 生育後期의 萎週

        朴鍾聲,金文圭,禹基大,劉勝憲 충남대학교 농업과학연구소 1977 農業技術硏究報告 Vol.4 No.1

        1. Chemical properties of soils and inorganic compositions of rice plants were analysed to explain the acute wilting occurred in the late stage of rice variety, Yusin. The results obtained were summarized as follows. 1) The amount of K and S_iO_2 of soils where severe rice wilting occurred was lower than that of soils where rice wilting didn't occurred. 2) The amount of N and FeO of wilted rice plants was higher than that of normal rice plants while the amount of K_2O and SiO_2 and the ratio of K_2O/N of former were lower than that of letter. 2. Severe wilting was occurred only in the rice variety, Yusin, when three varieties… Yusin, Milyang #15, Milyang #23……were interplanted. The amount of organic and inorganic compositions of three varieties and chemical properties of rhizosphere soils were analysed. 1) The rice variety, Yusin, was the lowest and that of the rice variety, Milyang #15, was the highest in the level of total carbohydrate and hemicellulose. 2) Comparatively higher levels of K were observed in rhizosphere soil where Yusin was planted, while the level of the K in the plant of Yusin was the lowest.

      • KCI등재

        연근의 열수 추출물이 흰쥐의 국소 뇌혈류량과 혈압에 미치는 영향

        박성혜,신언환,구재근,이태헌,한종현 동아시아식생활학회 2005 동아시아식생활학회지 Vol.15 No.1

        The purpose of this study was to measure the changes of regional cerebral blood flow(rCBF) and blood pressure(BP) in rats, following the intravenous injection of Nelumbo nucifera water extract. The measurement was continually monitored by laser-doppler flowmeter and pressure transducer in anesthetized adult Sprague-Dawley rats for about 2 to 2 and half hours through the data acquisition system composed of MacLab and Macintosh computer, The results of this experiment were as follows. Nelumbo nucifera significantly increased the changes of rCBF in rats. The rCBF of Nelumbo nucifera was not changed by pretreated propranolol, atropine, L-NNA and indomethacin. But the rCBF of Nelumbo nucifera was decreased by pretreated methylene blue. Nelumbo nucifera decreased the BP significantly, The BP of Nelumbo nucifera was not changed by pretreated propranolol, atropine, L-NNA and indomethacin. But the BP of Nelumbo nucifera was decreased by pretreated methylene blue. These results indicated that Nelumbo nucifera might increase the rCBF and decrease the BP which related to guanylyl cyclase activity.

      • 전자협상에서 협상스타일과 협상전술이 협상성과에 미치는 영향에 관한 실험연구 : An Experimental Study of Electronic Buyer-Supplier Negotiation

        박헌준,이종건,박성훈 한국협상학회 2002 협상연구 Vol.8 No.1

        본 연구는 구매-공급 양자간 전자협상에서 협상자의 위치에 따른 협상전술의 사용, 협상스타일과 협상전술 각각에 따른 협상성과의 차이를 살펴본다. 협상자의 위치는 구매자와 공급자, 협상전술은 일반적 협상전술과 기만적 협상전술, 협상스타일은 경쟁성향과 협력성향으로 구분하였다. 협상실험은 구매-공급자간 전자협상을 10일간 실시하였으며, 18개 구매-공급자 쌍 36명이 참여하였다. 분석결과, 구매자가 공급자보다 일반적 협상전술을 더 많이 사용하는 것으로 나타났으며, 구매자의 협상성과는 구매자의 일반적 협상전술의 사용이 낮은 경우보다 높은 경우에 더 높게 나타났다. 공급자에 있어서는, 공급자의 경쟁성향이 낮은 경우에는 협상성과가 높게 나타났으며, 경쟁성향이 높은 경우에는 협력성향이 높아야 협상성과가 높게 나타나는 것으로 밝혀졌다. 아울러, 본 연구의 연구결과에 대한 이론적·실무적 시사점을 논의하였다. This study examines the effects of negotiation style and bargaining tactics on negotiation outcomes of electronic buyer-supplier negotiation. Using three variables (buyer and supplier negotiators' position, general and deceptive bargaining tactics, competitive and cooperative negotiation style), thirty-six undergraduate students majoring in business formed 18 pairs and participated in electronic buyer-supplier negotiation for 10 days. Results show that buyers use general bargaining tactics more than suppliers and that buyers' negotiation outcomes are higher when they use more general bargaining tactics. Results also indicate that suppliers' low competitive style bring forth high negotiation outcomes and suppliers' high competitive style when used with cooperate style generate high negotiation outcomes. Theoretical and practical implications of this study are discussed.

      • Baculovirus를 利用한 Canine Parvovirus VP2蛋自質의 發現

        朴鍾賢,宋載永,玄芳動,安動濬,姜永源,全茂炯,安壽煥 충남대학교 생물공학연구소 1998 생물공학연구지 Vol.6 No.-

        국내에서 分離된 개파보바이러스주(V20주)의 VP2 遺傳子를 baculovirus system을 이용하여 발현시켜 다음의 結果를 얻었다. 1. 개파보바이러스의 VP2 유전자를 PCR에 의해 增幅하여 1755bp의 VP2遺傳子를 pUC19에 클로닝하여, 클로닝된 遺傳子를 polyhedrin promoter를 가지는 baculovirus expression vector인 pVL1393에 옮겨 VP2 발현벡터인 pVL1393-VP2를 얻을 수 있었다. 2. pVL1393-VP2 plasmid와 baculovirus DNA와의 homologous recombination에 의해 재조합바이러스인 VP2-BV를 얻을 수 있었으며, 그 발현효율은 2.000-5.000 HAU/0.05 ml이었다. 3. 免疫沈澱法에 의해 발현된 단백질은 개파보바이러스의 VP2단백질과 유사한 64 kb에 달하는 것이었으며, 血球凝集能을 지니고 있었다 4. 血球凝集能을 지닌 蛋白質이 여러 陽性血淸에 있어서 抗體수준을 測定할 수 있는지 개파보바이러스항원과의 相關性을 比較한 바 0.94 (n=125. p<0.01)의 相關係數를 보였다 5. 발현 VP2白은 virus-like particles를 形成하였으며, 그 크기로는 개파보바이러스와 비슷한 25 ㎚의 크기를 갖았다. Canine parvovirus(CPV) is a member of autonomous replicating parvoviruses and is aetiologically associated with enteritis and mycoarditis in puppies. The capsids of CPV are composed of three structural proteins: VP1, VP2 and VP3. The VP2 protein is the major component of capsid. The VP2 gene of a canine parvovirus. V20 strain isolated in Korea was cloned into baculovirus expression vector, and subsequently the VP2 protein was expressed by a recombinant baculovirus under the control of polyhedrin promoter. The recombinant VP2 protein expressed in Spodoptera frugiperda 9(Sf9) cells was detected by haemagglutination(HA) test and immunofluorescent antibody assay. Molecular weight of the recombinant VP2 protein expressed was estimated as 64Kd when tested by immunoprecipitation test using anti-CPV monoclonal antibody. In haemagglutination inhibition(Ⅲ) test. 8 HA units of the recombinant VP2 protein antigen was successfully utilized to determine a level of antibody against CPV in various positive sera. The recombinant VP2 protein showed also the capability to form virus like particles similar in size and appearance to the CPV virions.

      • 경주지역 서비스산업에 대한 서비스 만족도 조사

        박종희,하헌국 동국대학교 관광산업연구소 1999 관광·호텔경영연구 Vol.1 No.1

        This study investigated the service quality level of service industry in Kyoungju. Ten different service categories were selected for this study: hotel, restaurant, bank, taxi, local bus, express bus, airlines, hospital, private education institute, and community bureau. To test service quality, five dimensions of service were used: reliability, responsiveness, assurance, empathy and tangibles. In general, people in Kyoungju perceived the service quality of Kyongju as poor. In particular, among 10 different service industry, they evaluated the services of taxi and local bus industry as worst. Tangibles, reliability, assurance, responsiveness and empathy were listed in order of declining relative satisfaction to customers. The main reason of the poor service in Kyoungju is the management's lack of understanding the importance of service.

      • 카드뮴이 마우스 뇌에서 아연의 항상성에 관여하는 유전자발현에 미치는 영향

        박종안,여은영,남상훈,장봉기,이종화,김완종 한국환경독성학회 2004 환경독성보건학회지 Vol.19 No.4

        Metallothionein (MT), a small protein molecule which can bind of release metal tons, is involved in the regulation of cellular metal homeostasis This study was investigated the accumulation of cadmium in blood, tissue (liver, kidney and brain), and the effect of cadmium on several key genes (MT-Ⅰ, MT-Ⅱ, ZnT-1) in zinc metabolism m the mouse Mouses weighing 20-25g were randomly assigned to control and cadmium treated group (Cd group) Cd group was intraperitoneally injected with cadmium 2, 4, 8 mg/kg and control group was administerd with saline Mouses of each group were sacrificed by decapitation 4 hours after the administration of cadmium Cadmium contents in blood, fiver, kidney and brain were increased by a dose-dependent manner Accumulation of cadmium was mainly occurred in liver and kidney Induction of MT-I and MT-11 protein was increased, but ZnT-1 expression was decreased to a dose-dependent manner by the treatment of 2~8mg/kg cadmium These results suggested that cadmium can be transported to brain and alter the expression of several key genes in zinc homeostasis.

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