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Surveillance and distribution of scrub typhus vectors, chigger mites in South Korea
Jong Yul Roh,E Hyun Shin,Young Ran Ju 한국응용곤충학회 2015 한국응용곤충학회 학술대회논문집 Vol.2015 No.10
To clarify the geographical distribution of scrub typhus vectors in Korea, the first survey of chigger mites was conducted from 2005 to 2007 by collecting wild small mammals twice a year (spring and autumn) at 24 sites nationwide. The two predominant mite species were Leptotrombidium pallidum (52.6%) and L. scutellare (27.1%). However, the proportions of L. scutellare in southern areas, including endemic provinces such as Chungcheongnam-Do, Jeollabuk-Do, Jeollanam-Do, and Gyeongsangnam-Do, were relatively higher than in central Korean regions where L. pallidum was predominant. In autumn, the ratio of L. scutellare increased to 42% while the ratio of L. pallidum decreased. The geographical distribution map of the L. scutellare chigger index was identical to the incidence pattern of scrub typhus, whereas those of overall mites and L. pallidum showed no relationship with case incidence patterns. Distribution mapping analysis shows an identical geographical distribution of L. scutellare and epidemic incidence of scrub typhus in South Korea. The second periodical survey was performed from 2011 to 2013. The result suggests that the distribution of L. scutellare has not been changed remarkably in comparison to the first survey.
( Jong Yul Roh ),( Ming Shun Li ),( Jin Hee Chang ),( Jae Young Choi ),( Hee Jin Shim ),( Sang Chul Shin ),( Kyung Saeng Boo ),( Yeon Ho Je ) 한국잠사학회 2007 International Journal of Industrial Entomology Vol.8 No.1
Expression of a fusion protein between B. thuringiensis crystal protein, Cry1Ac1 and a scorpion insect toxin (AaIT, Androctonus australis Hector insect toxin) in acrystalliferous B. thuringiensis strain (Cry B strain) was examined. The cry1Ac1 gene was cloned in B. thuringiensis-E. coli shuttle vector, pHT3101, under the control of the native cry1Ac1 gene promoter (pProAc) and a gene encoding AaIT was inserted in XhoI site in the middle of the cry1Ac1 gene (pProAc-ScoR). B. thuringiensis Cry-B strain carrying pProAc-ScoR (ProAc-ScoR/CB) produced an inclusion body of irregular shape and the expressed fusion protein is approximately 65 kDa in size. Sporulated cells and spore-crystal mixtures of ProAc-ScoR/CB had insecticidal activity against Plutella xylostella larvae, showing LT50 of ProAc-ScoR/CB (22.59 hrs) lower than that of ProAc/CB (30.06 hrs) at 1×10(7) CFU/cm2. These results suggest that the fusion protein including a B. thuringiensis crystal protein and an AaIT may be functionally expressed in B. thupingiensis. Moreover, we verified the additive toxicity of AaIT, which is a new feasible candidate for insect control.
Roh, Jong-Yul,Li, Ming-Shun,Chang, Jin-Hee,Park, Jae-Young,Shim, Hee-Jin,Woo, Soo-Dong,Boo, Kyung-Saeng,Je, Yeon-Ho Korean Society of Sericultural Science 2003 International Journal of Industrial Entomology Vol.6 No.1
To verify importance of the intervening sequence between the ribosome binding site (RBS) and the initiation codon for expression of Bacillus thuringiensis $\delta$-endotoxin, the pProMu, containing SphI and NcoIsites between RBS and the initiation codon of the cry1Ac gene, and the deletion derivatives of pProMu were constructed and transformed into the B. thuringiensis subsp. kurstaki $Cry^{-B}$ strain. The pProMu-ΔSphIhad identical six bases of intervening sequence to pProAc though the arrangement of sequence was different. Other mutants containing pProMu had 1 or 10 or 14 bases between RBS and the initiation codon. Among deletion mutants, only ProMu-ΔSphI/CB only produced 130 kDa typical bipyramidal crystals like those seen for ProAc/CB. However, ProMu/CB, $ProMu-{\Delta}NcoI$, and ProMu-ΔSphI+NcoIdid not produce Cry1Ac crystals. In conclusion, the results suggest that 6-base intervening sequence was important for expression of cry1-type class gene. Furthermore, spacing effect of the intervening sequences may play an important role in expression of individual crystal proteins in B. thuringiensis without doubt.
Simple Purification of a Foreign Protein Using Polyhedrin Fusion in a Baculovirus Expression System
ROH, Jong Yul,CHOI, Jae Young,KANG, Joong Nam,WANG, Yong,SHIM, Hee Jin,LIU, Qin,TAO, Xueying,XU, Hong Guang,HYUN, Jin-Ho,WOO, Soo Dong,JIN, Byung Rae,JE, Yeon Ho Japan Society for Bioscience, Biotechnology, and A 2010 Bioscience, Biotechnology, and Biochemistry Vol.74 No.8
<P>Previously, we found that expression by translational fusion of the polyhedrin (Polh)-green fluorescence protein (GFP) led to the formation of granular structures, and that these fluorescent granules were easily precipitated by high-speed centrifugation. Here, we developed an easy, fast, mass purification system using this baculovirus expression system (BES). An enhanced GFP (EGFP) fused with the Polh gene at the N-terminus, including a linker and enterokinase (EK) site between Polh and EGFP, was expressed in Sf9 cells. The cells infected by AcPolhEKA-EGFP produced fluorescent granules. The EGFP fusion protein was purified from granule-containing cells in three steps: cell harvest, sonication, and EK digestion. Through final enterokinase digestion, EGFP presented mainly in the supernatant, and this supernatant fraction also showed a pure EGFP band. These results suggest that a combined procedure of Polh fusion expression and enterokinase digestion can be used for rapid and easy purification of other proteins.</P>
Roh, Jong Yul,Je, Yeon Ho,Park, Hyun Woo,Chang, Jin Hee,Jin, Byung Rae,Lee, Dae Weon,Ziwen Yang,Kang, Seok Kwon 한국잠사학회 1998 한국잠사곤충학회지 Vol.40 No.2
A noninsecticidal strain, Bacillus thuringiensis NTB-88, isolated from Korean soil, had a typical bipyramidal parasporal inclusion and its serotype is identical to B. thuringiensis subsp. Morrisoni(H8a8b). To elucidate differences between insecticidal and noninsecticidal strains, we compared strain NTB-88 to other toxic B. thuringiensis subsp. morrisoni strains (HD-12 and PG-14). Restriction endonucleases digested plasmid DNA patterns showed that strain NTB-88 was different from lepidopteran-toxic strain, HD-12, but it was similar to dipteran-toxic strain, PG-14. The gene type of strain NTB-88 was different from those of other insecticidal strains. Furthermore, the NH2-terminal amino acid sequence of crystal protein of strain NTB-88 had no relation to those of the previously known δ-endotoxins in other toxic strains as well as HD-l2 and PG-14 strains. Therefore, the noninsecticidal crystal protein in strain NTB-88 is novel and its property is different from insecticidal ones.
( Jong Yul Roh ),( Ming Shun Li ),( Jin Hee Chang ),( Hee Jin Shim ),( Byung Rae Jin ),( Kyung Saeng Boo ),( Yeon Ho Je ) 한국잠사학회 2007 International Journal of Industrial Entomology Vol.8 No.2
The expression of a fusion protein comprised of the B. thuringiensis crystal protein, Cry1Ac, and enhanced green fluorescent protein (EGFP) in Escherichia coli XLI-blue was examined. Three recombinant plasmids were transformed into E. coli XL1-blue and named as ProAc/Ec, MuEGFP/Ec and ProMu-EGFP/Ec, respectively. All transformants were observed by light and fluorescence microscopy at mid-log phase. The expression in E. coli transformants, ProMu-EGFP/Ec and MuEGFP/Ec, exhibited bright enough fluorescence to be observed. Furthermore, ProMu-EGFP/Ec produced fluorescent inclusions, which may have been recombinant crystals between EGFP and Cry1Ac while MuEGFP/Ec expressed soluble EGFP in cell. In SDS-PAGE, ProAc/Ec had 130 kDa crystal protein band and MuEGFP/Ec had thick 27 kDa EGFP band. However, ProMu-EGFP/Ec had about 150 kDa fusion protein band. Accordingly, these results indicated that a fusion protein between the B. thuringiensis crystal protein and a foreign protein under the lacZ promoter was successfully expressed as granular structure in E. coli. It is suggested that the E. coli expression system by N-terminal fusion of B. thuringiensis crystal protein may be useful as excellent means for fusion expression and characterization of B. thuringiensis fusion crystal protein.