내시경적 절제로 진단된 폴립 형태의 심재성 낭종성 위염 2예

허종현,조영화,성용완,유재훈,구동영,김갑식,노지훈,문원 고신대학교의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.2

Gastritis cystica profunda (GCP) is a disease characterized by hyperplastic and significant extension of cystic dilatation of the gastric mucous glands, which results in a cystic lesion in gastric submucosa. It often occurs on the part of gastroenterostomy, but can be found in the stomach without any previous surgery. GCP has variable gross finding including solitary polyps, diffuse ones, submucosal tumors, and rare giant gastric mucosal fold. It is difficult to tell GCP from a cancerous lesion by gross finding that the disease demands a necessary tissue biopsy, though GCP is commonly showed as a benign in the progress. We report a case of GCP in polypoid types diagnosed by endoscopic polypectomy.

내시경적 밴드 결찰술로 치료한 출혈을 동반한 위 혈관이형성증 1예

김갑식,허종현,성용완,조영화,구동영,유재훈,노지훈,문원 고신대학교의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.2

Gastric angiodysplasia is vascular ectasia in mucosa and submucosa of the gastric wall. It is an uncommon cause of upper gastrointesinal hemorrhage that may occur in the stomach or duodenum. There are many kinds of endoscopic treatments, such as argon plasma coagulation, electrocoagulation, heat probe, and submucosal injection of hypertonic saline mixed with epinephrine for gastric angiodysplasia. However, these treatment methods are associated with high rate of rebleeding. In order to avoid the recurrence, endoscopic band ligation has recently been used as an alternative method for endoscopic treatment of gastric angiodysplasia. We encountered a case of gastric angiodysplasia that presented with overt bleeding and anemia, and was successfully treated with endoscopic band ligation.

흰민들레 (Taraxacum coreanum) 추출물이 급성 수은 중독된 생쥐의 간에 미치는 효과

정민주,윤중식,허진,노영복,최영복,김종세,이현화 韓國電子顯微鏡學會 2008 Applied microscopy Vol.38 No.1

본 연구는 급성 수은 독성에 대한 민들레의 효과를 알아보기 위하여 시도되었다. 30 g 내외의 생쥐를 대상으로 대조군, 수은(5mg/kg) 투여군, 수은 투여 후 민들레(3 g/kg)를 구강투여 한 후 다시 24, 48, 72, 96시간, 1주일군으로 세분하여 간장 손상 억제 효과를 알아보기 위하여 생화학적 및 조직학적 실험을 실시하였다. 혈액중 aspartate amiotransferase (AST)와 alanine aminotransferase (ALT)의 수치는 민들레 투여군이 수은 투여군보다 감소되었다. 또한 간조직의 SOD와 catalase 활성도 역시 민들레 투여군이 수은 투여군에 비하여 감소하였으나 통계학적 유의성은 없었다. 간 조직의 광학현미경적 관찰에서 심한 조직괴사가 관찰되지만 민들레 투여군에서는 문맥주위의 약간의 괴사와 심한 호중구 침윤현상이 관찰되었다. 전자현미경적 관찰 결과, 간장에서 수은 투여군은 간세포의 핵이 함입되어 불규칙했으며 미토콘드리아와 조면소포체의 수조가 팽대되고, 리보솜의 탈락이 관찰되었다. 민들레 투여군은 핵이 정상적인 상태로 관찰되었고, 전자밀도가 높은 미토콘드리아가 분포되어 있었으며, 리보솜이 부착된 상태로 층판구조를 형성하는 조면소포체가 관찰되었다. 이상의 연구 결과로 보아 민들레가 수은으로 유발된 간손상을 보호하는 효과가 있는 것으로 사료되며 민들레에 대한 다양한 연구가 필요할 것으로 사료된다. Dandelion has been frequently used as a remedy for women’s disease, inflammatory diseases and disorders of the liver and gallbladder. Dandelion extracts water extract, an herbal medication, may have an effect on the activity of hepatic antioxidant enzymes in diabetic rat. This study aims demonstrate the effect of dandelion extracts, one of the natural chelator, on the biochemical and enzyme activity changes in the mouse liver caused by HgCl₂. Mice approximately 30 gm in weight were grouped into the control, mercury chloride-treated, and the dandelion extractstreated after mercury chloride groups. HgCl₂ (5 mg/kg) and dandelion extracts (3 g/kg) were delivered orally. Serum AST and ALT were measured, enzyme activity of liver were examined by spectrophotometer and ultrastructural alteration of liver were examined by light and electron microscopy. Dandelion extracts were decreased the increase of serum AST and ALT level induced by mercury. The catalase activity was decreased in the dandelion extracts group. The activity of SOD was dereased, but did not show significant differences. Mercury chloride-treated hepatic cell were irregular nucleus, enlarged and reduced number of mitochodria, enlarged rough endoplasmic reticulum, loss of ribosomes. Cells treated with dandelion extracts were similar to those of the control group. In conclusion, dandelion extracts may protect the mercury-induced toxicity on Liver.

"2보 전진과 1보 후퇴"-기업과실치사 및 기업살인법에 관하여 울리는 경종

노종화 ( Jong Hwa Roh ),강태승 ( Tae Seung Kang ) 서울대학교 BK21 법학연구단 공익인권법연구센터 2014 공익과 인권 Vol.14 No.-

Chromosomal cephalosporinase in Enterobacter hormaechei as an ancestor of ACT-1 plasmid-mediated AmpC β-lactamase

Roh, Kyoung Ho,Song, Wonkeun,Chung, Hae-Sun,Lee, Yang Soon,Yum, Jong Hwa,Yi, Ha Na,Chun, Jong Sik,Yong, Dongeun,Lee, Kyungwon,Chong, Yunsop Microbiology Society 2012 Journal of medical microbiology Vol.61 No.1

<P>In this study of the diversity of AmpC β-lactamase in clinical isolates of Enterobacter spp., a strain was found carrying the plasmid-mediated AmpC β-lactamase ACT-1 gene on its chromosome. The strain was identified as Enterobacter hormaechei using phylogenetic analysis of 16S rRNA and hsp60 genes. In addition, the species was confirmed by DNA-DNA hybridization. The genetic environment of the bla(ACT-1) gene was characterized, including the ampR and ampG genes, using a two-step PCR. The amino acid sequences of AmpR at serine 35, arginine 86, glycine 102, aspartic acid 135 and tyrosine 264 were conserved. Measurement of the transcription level of the bla(ACT-1) gene using real-time quantitative PCR showed that it increased 1.98-fold following cefoxitin induction. These results suggest that the plasmid-mediated bla(ACT-1) gene originated from the chromosome of E. hormaechei.</P>

Decrease in Carbamylation of Rubisco by High CO, Concentration is Due to Decrease of Rubisco Activase in Kidney Bean

Roh, Kwang Soo,Kim, In Seob,Kim, Byung Weon,Song, Jong Suk,Chung, Hwa Sook,Song, Seung Dal 한국식물학회 1997 Journal of Plant Biology Vol.40 No.2

Decrease in rubisco activation at high CO_2 concentration was caused by decrease in carbamylation of rubisco (Roh et al., 1996). However, it is unclear whether decrease in carbamylation rate at high CO_2 concentration is due to decrease in activity itself or content of rubisco activase. To clarify this ambiguity, investigation was performed to determine effects of CO_2 concentration on rubisco activase with kidney bean (Phaseolus vulgaris L.) leaves grown at normal CO_2 (350 ppm) and high CO_2 (650 ppm) concentration. The analysis of Western blotting showed that the 50 and 14.5 kD polypeptides were identified immunochemically as the large and small subunits of rubisco in the preparation, respectively. For the 14.5 kD small subunit, the degree of intensity at high CO_2 concentration was similar to that at normal CO_2 concentration. For the 50 kD large sububit, however, the intensity of a band at high CO_2 concentration was significantly higher than that at normal CO_2 concentration, indicating that only the large subunit is affected by high CO_2 concentration. The analysis of Western immunoblotting showed two major polypeptides at 46 and 42 kD which were identified as rubisco activase subunits. The intensities of two bands were shown to be higher at normal CO_2 than high CO_2 concentration. These data indicate that decrease of carbamylation resulting from increase of CO_2 concentration was caused by rubisco activase. Finally, by employing ATP hydrolysis assay and ELISA, we also observed a significant decrease in both activity and content of rubisco activase as CO_2 concentration was raised from normal to high Co_2 concentration. These results suggest that decrease in rubisco carbamylation at high CO_2 concentration is caused by activity itself and/or content of rubisco activase.

Decrease of the Activation and Carbamylation of Rubisco by High CO₂ in Kidney Bean

Roh, Kwang Soo(노광수),Jae Ki Kim(김재기),Seung Dal Song(송승달),Hwa Sook Chung(정화숙),Jong Suk Song(송종석) 한국생물공학회 1996 KSBB Journal Vol.11 No.3

Influence of Benomyl on Photosynthetic Capacity in Soybean Leaves

Roh, Kwang-Soo,Oh, Mi-Jung,Song, Seung-Dal,Chung, Hwa-Sook,Song, Jong-Suk The Korean Society for Biotechnology and Bioengine 2001 Biotechnology and Bioprocess Engineering Vol.6 No.2

This investigation was performed to study the influence of benomyl on photosynthetic pigments and enzymes in soybean leaves. Chlorophyll and pheophytin levels were reduced by benomyl 45 days after greening. These results indicate that chlorophyll a and b, and pheophytin must be controlled by benomyl. SDS-PAGE analysis showed that 50 and 14.5 kD polypeptides represented as large and small subunits of rubisco. In the both of these subunits, the band intensity of the control was significantly higher than that after benomyl treatment, indicating that these two subunits are affected by benomyl. Benomyl strongly inhibited both the activity and content of rubisco as its concentration was gradually increased. However, it remains unclear whether this reduction of rubisco level was due to a reduced level of rubisco activase. Two major polypeptides of 46 and 42 kD were identified as rubisco activase subunits by SDS-PAGE. The intensity of these two bands was shown to be higher in the control than after benomyl treatment. These results indicate that the rubisco decrease resulting from increased benomyl concentrations was caused by rubisco activase. A significant decrease in both the activity and content of rubisco activase by benomyl was also observed. There results suggest that the decrease in rubisco level caused by benomyl is accompanied by a decrease in both the activity and content of rubisco activase.

Immunoblot Analysis of the Expression of Genes for Barley Rubisco Activase in E. coli

Roh, Kwang Soo,Kwon, Mo Sun,Do, Young Hwan,Song, Seung Dal,Chung, Hwa Sook,Song, Jong Suk 한국식물학회 1998 Journal of Plant Biology Vol.41 No.3

Rubisco activity during photosynthesis is regulated by the rubisco activase, which facilitates the dissociation of RuBP and other inhibitory sugar phosphates from the active site of rubisco in an ATP-dependent reaction. In this paper, barley Rca genes (RcaA1, RcaA2 and RcaB) were expressed in E. coli and the activity of rubisco activase expressed was assayed biochemically by chromatography. Then the protein was identified electrophoretically by SDS-PAGE and detected immunologically by Western blot analysis using polyclonal antibodies raised against the kidney bean rubisco activase as probe. The band pattern of purified proteins on the polyacrylamide gel showed two polypeptides of 46 kD and 42 kD. Anti-rubisco activase antibodies reacted specifically with both polypeptides of 46 kD and 42 kD present in the crude extracts of E. coli transformants. Therefore, it was found that the genes of barley rubisco activase was successfully expressed in E. coli as active forms of 46 kD and 42 kD.

Exogenous GA_3 Increases Rubisco Activation in Soybean Leaves

Roh, Kwang Soo,Im, Eun Jung,Yeo, Sang Eun,Oh, Mi Jung,Song, Jong Suk,Chung, Hwa Sook,Song, Seung Dal 한국식물학회 2001 Journal of Plant Biology Vol.44 No.1

We studied the effect of exogenous applications of GA_3 on rubisco activation in soybean leaves. Activity at 0.1μM GA_3 was significantly greater than in leaves receiving no treatment Rubisco content snowed patterns of change similar to that for activity. These data suggest that activity was associated with the amount of rubisco protein, and that the activation and induction of rubisco was promoted by GA_3. The degree of intensity of the 50- and 14.5-kD polypeptides (identified as the large and small subunit, respectively, of rubisco by SDS-PAGE analysis) at 0.1μM GA_3 was significantly higher than that for the control, indicating that GA_3 affected both subunits. The stimulation effects of rubisco activation by GA_3 seem to be caused by the expression of rubisco genes at the transcriptional level. Assuming that these GA_3 effects were related to rubisco activase, we also determined the activity and content of that enzyme. Its activity at 0.1μM GA_3 increased more than did the control. A similar pattern of change was observed for rubisco activase content The intensity of two 46- and 42-kD polypeptide bands at GA_3 was higher than for the corresponding bands at the control. Therefore, the change in rubisco activase levels may lead to altered levels of rubisco.