http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
둔상에 의해 발생한 잠복기흉의 치료방침 (컴퓨터 단층 촬영 소견에 의한 분류)
조진성 ( Jo Jin Seong ),임용수 ( Im Yong Su ),이근 ( Lee Geun ),현성열 ( Hyeon Seong Yeol ),진욱 ( Jin Ug ),황성연 ( Hwang Seong Yeon ) 대한외상학회 2003 大韓外傷學會誌 Vol.16 No.1
Background: Occult pneumothorax defined as a pneumothorax that is detected by computed tomography (CT) scaning, not routine supine screening chest roentgenograms. Optimal treatment for blunt trauma occult pneumothoraces has not been defined. Methods: Ches
Leveraging Evolutionary Protein Engineering for Efficient Production of Itaconate from Acetate
Jo Hyun MOON,Myung Hyun NOH,Hyun Gyu LIM,Jae-Seong YANG,Gyoo Yeol JUNG 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Utilizing acetate as a carbon source is a promising approach for sustainable development, as it is obtainable from abundant sources. In previous studies, conversion of acetate into itaconate was conducted with rational metabolic engineering strategy, proving the potential of acetate as a promising carbon source. Itaconate, a versatile building block of various valuable products, is produced by cis-aconitate decarboxylase (cad) in one step reaction. However, there are hurdles when expressing cad in E. coli, as it has low activity when heterologously expressed and the pool of substrate, cis-aconitate, is low as it is the intermediate of TCA cycle. In this study, we leveraged evolutionary protein engineering based on recently identified structure of Cad for improving its properties and itaconate high-throughput screening sensor (HTS) for screening the Cad variants with better performances. Thereafter, curing of itaconate HTS sensor were conducted for validation of the screened Cad variants and found out that the screened mutants VR and GT showed 1.28-fold increase in itaconate titer. The results of enzyme assay demonstrated that the mutants VR and GT had 1.25-fold higher catalytic efficiency compared to wild type Cad, even though affinity was slightly lower (Km value 1.42-fold increase). The mutants will be additionally validated in final production strain to maximize the itaconate production.