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Jiong-feng Zhao,Tao-Wang,Jianping Lin,Lirong Yang,Mian-bin Wu 한국생물공학회 2019 Biotechnology and Bioprocess Engineering Vol.24 No.2
Early research on the nutritional value of 1,3- diacylglycerols (1,3-DAGs) has resulted in a significant interest in their synthesis. 1,3-DAGs can be produced chemically and biologically. In this work, a regioselective lipase from Rhizopus oryzae was efficiently immobilized on nanosized magnetite particles (NSM) in an oriented way, resulting in significant enhancement of activity. The specific hydrolytic and esterification activities of the immobilized enzyme were 1,660% and 260% of those of the free enzyme, respectively. The immobilized enzyme was then used to catalyze the esterification of oleic acid with glycerol in a solvent-free system for preparation of 1,3-DAG in a 1 L reactor. The catalytic process was studied in detail, the final concentration of 1,3-DAG reached >76% under the optimal condition when the molar ratio of oleic acid to glycerol was 2.8:1. The regioselectivities of free and immobilized enzyme were both >97%. The immobilized enzyme was reused for 55 cycles with only ~30% activity loss at 30ºC. The purity of 1,3-DAG was up to ~95% (w/w) after a simple purification step with the recovery ratio ~85%. This is the first report of efficient 1,3-DAG purification by neutralization without acyl migration.
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Lan-Lan Yan,Jiong-Feng Liang,Yan-gang Zhao 사단법인 한국계산역학회 2019 Computers and Concrete, An International Journal Vol.23 No.3
The use of recycled aggregate concrete for the purpose of environmental and resource conservation has gained increasing interest in construction engineering. Nevertheless, few studies have reported on the bonding performance of the bars in recycled aggregate concrete after exposed to high temperatures. In this paper, 72 pull-out specimens and 36 cubic specimens with different recycled coarse aggregate content (i.e., 0%, 50%,100%) were cast to evaluate the bond behavior between recycled aggregate concrete and steel bar after various temperatures (20°C, 200°C, 400°C, 600°C). The results show that the recycled aggregate concrete pull-out specimens exhibited similar bond stress-slip curves at both ambient and high temperature. The bond strength declined gradually with the increase of the temperature. On the basis of a regression analysis of the experimental data, a revised bond strength mode and peak slip ratios relationship model were proposed to predict the post-heating bond-slip behavior between recycled aggregate concrete and steel bar.
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