Expression of selenium-independent glutathione peroxidase 5 (GPx5) in the epididymis of Small Tail Han sheep

Li, Ruilan,Fan, Xiaomei,Zhang, Tong,Song, Huizi,Bian, Xiaona,Nai, Rile,Li, Jinquan,Zhang, Jiaxin Asian Australasian Association of Animal Productio 2018 Animal Bioscience Vol.31 No.10

Objective: Selenium-independent glutathione peroxidase (GPx5) is specifically expressed in the mammalian epididymis and plays an important role in protecting sperm from reactive oxygen species and lipid peroxidation damage. This study investigates GPx5 expression in the epididymis of Small Tail Han sheep. Methods: GPx5 expression was studied in three age groups: lamb (2 to 3 months), young (8 to 10 months), and adult (18 to 24 months). The epididymis of each age group divided into caput, corpus and cauda, respectively. Analysis the expression quantity of GPx5 in epididymis and testis by real-time fluorescent quantitative polymerase chain reaction and Western blot. Finally, GPx5 protein locating in the epididymis by immunohistochemical. Results: The results demonstrate that in the lamb group, the GPx5 mRNA, but not protein, can be detected. GPx5 mRNA and expressed protein were detected in both the young and adult groups. Moreover, both the mRNA and protein levels of GPx5 were significantly higher in the young group than in other two groups. When the different segments of epididymis were investigated, GPx5 mRNA was expressed in each segment of epididymis regardless of age. Additionally, the mRNA level in the caput was significantly higher than that in corpus and cauda within same age group. The GPx5 protein was in the epithelial cells' cytoplasm. However, GPx5 mRNA and protein were not detected in the testis. Conclusion: These results suggest that GPx5 is mainly expressed in the epididymis of Small Tail Han sheep, and that the expression level of GPx5 is associated with age. Additionally, GPx5 was primarily expressed in the epithelial cells of the caput. Taken together, these studies indicate that GPx5 is expressed in the epididymis in all age grades.

Analysis of shear lag effect in the negative moment region of steel-concrete composite beams under fatigue load

Jinquan Zhang,Bing Han,Hui-bing Xie,Wu Tong Yan,Wangwang Li,Jiaping Yu 국제구조공학회 2021 Steel and Composite Structures, An International J Vol.39 No.4

Shear lag effect was a significant mechanical behavior of steel-concrete composite beams, and the effective flange width was needed to consider this effect. However, the effective flange width is mostly determined by static load test. The cyclic vehicle loading cases, which is more practical, was not well considered. This paper focuses on the study of shear lag effect of the concrete slab in the negative moment region under fatigue cyclic load. Two specimens of two-span steel-concrete composite beams were tested under fatigue load and static load respectively to compare the differences in the negative moment region. The reinforcement strain in the negative moment region was measured and the stress was also analyzed under different loads. Based on the OpenSees framework, finite element analysis model of steel-concrete composite beam is established, which is used to simulate transverse reinforcement stress distribution as well as the variation trends under fatigue cycles. With the established model, effects of fatigue stress amplitude, flange width to span ratio, concrete slab thickness and shear connector stiffness on the shear lag effect of concrete slab in negative moment area are analyzed, and the effective flange width ratio of concrete slab under different working conditions is calculated. The simulated results of effective flange width are compared with calculated results of the commonly used specifications, and it is found that the methods in the specifications can better estimate the shear lag effect in concrete slab under static load, but the effective flange width in the negative moment zone under fatigue load has a large deviation.

Mutant IDH1 Enhances Temozolomide Sensitivity via Regulation of the ATM/CHK2 Pathway in Glioma

Lin Lin,Jinquan Cai,Zixiao Tan,Xiangqi Meng,Ruiyan Li,Yang Li,Chuanlu Jiang 대한암학회 2021 Cancer Research and Treatment Vol.53 No.2

Purpose Isocitrate dehydrogenase 1 (IDH1) mutations are the most common genetic abnormalities in low-grade gliomas and secondary glioblastomas. Glioma patients with these mutations had better clinical outcomes. However, the effect of IDH1 mutation on drug sensitivity is still under debate. Materials and MethodsIDH1-R132H mutant cells were established by lentivirus. IDH1-R132H protein expression was confirmed by western blot. The expression of ataxia telangiectasia mutated (ATM) signaling pathway and apoptosis-related proteins were detected by immunofluorescence and western blot. Temozolomide (TMZ) induced cell apoptosis was detected by flow cytometry. Tumor cell proliferation was detected by Cell Counting Kit-8. In vivo nude mice were used to confirm the in vitro roles of IDH1 mutation.ResultsWe established glioma cell lines that expressed IDH1-R132H mutation stably. We found that TMZ inhibited glioma cells proliferation more significantly in IDH1 mutant cells compared to wild type. The IC50 of TMZ in IDH1-R132H mutant group was less than half that of wild-type group (p < 0.01). TMZ significantly induced more DNA damage (quantification of γH2AX expression in IDH1 mutation vs. wild type, p < 0.05) and apoptosis (quantification of AnnexinV+propidium iodide–cells in IDH1 mutation versus wild type, p < 0.01) in IDH1 mutant gliomas compared to wild-type gliomas. The ATM-associated DNA repair signal was impaired in IDH1 mutant cells. Inhibiting the ATM/checkpoint kinase 2DNA repair pathway further sensitized IDH1 mutant glioma cells to chemotherapy. We found that IDH1 mutation significantly inhibited tumor growth in vivo (the tumor size was analyzed statistically, p < 0.05). Moreover, we confirmed that gliomas with IDH1 mutation were more sensitive to TMZ in vivo compared to wild type significantly and the results were consistent with the in vitro experiment. ConclusionThese results provide evidence that combination of TMZ and ATM inhibitor enhances the antitumor effect in IDH1 mutant gliomas.

Adverse effect of DEHP exposure on the serum insulin level of Balb/c mice

Jing Wang,Jinquan Li,Kashif Rafiq Zahid,Kun Wang,Yan Qian,Ping Ma,Shumao Ding,Xu Yang,Xianliang Wang,Xianliang Wang,Xu Yang 대한독성 유전단백체 학회 2016 Molecular & cellular toxicology Vol.12 No.1

Di(2-ethylhexyl) phthalate (DEHP) is a common indoor pollutant in the world, which may cause lots of harmful effects in human including diabetes according to epidemiological studies. To explore the underlying role of DEHP in diabetes-like symptoms, Balb/c mice were chose to be the experimental animals in this paper. They were separated as eight groups as follows: (1) saline+normal diet (vehicle control), (2) 10 mg/kg.day DEHP+normal diet, (3) 50 mg/kg.day DEHP+normal diet, (4) 250 mg/kg.day DEHP+normal diet, (5) streptozotocin (STZ)+high fat diet (diabetes model), (6) 10 mg/kg.day DEHP+ STZ+high fat diet, (7) 50 mg/kg.day DEHP+STZ +high fat diet, (8) 250 mg/kg.day DEHP+STZ+high fat diet. The biomarkers of this experiment include four groups: (1) general indicates: body weight and drinking water, (2) blood biomarkers: serum insulin and fasting glucose, (3) pathological examination: pancreas section and kidney section, and (4) biomarkers of oxidative stress: reactive oxygen species (ROS) and malondialdehyde (MDA) in liver cells. Our study results demonstrate that: (1) at our treatment levels DEHP cannot directly induce diabetes, but reduce serum insulin level in DEHP-exposed non-STZ-treated animals, (2) pathological examination finds that these is a dose-dependent damage in the pancreas in DEHP- exposed STZ-treated groups, and (3) the oxidative mechanism may be involved in this pathological process.

Expression of fox-related genes in the skin follicles of Inner Mongolia cashmere goat

Wenjing Han,Xiaoyan Li,Lele Wang,Honghao Wang,Kun Yang,Zhixin Wang,Ruijun Wang,Rui Su,Zhihong Liu,Yanhong Zhao,Yanjun Zhang,Jinquan Li 아세아·태평양축산학회 2018 Animal Bioscience Vol.31 No.3

Objective: This study investigated the expression of genes in cashmere goats at different periods of their fetal development. Methods: Bioinformatics analysis was used to evaluate data obtained by transcriptome sequencing of fetus skin samples collected from Inner Mongolia cashmere goats on days 45, 55, and 65 of fetal age. Results: We found that FoxN1, FoxE1, and FoxI3 genes of the Fox gene family were probably involved in the growth and development of the follicle and the formation of hair, which is consistent with previous findings. Real-time quantitative polymerase chain reaction detecting system and Western blot analysis were employed to study the relative differentially expressed genes FoxN1, FoxE1, and FoxI3 in the body skin of cashmere goat fetuses and adult individuals. Conclusion: This study provided new fundamental information for further investigation of the genes related to follicle development and exploration of their roles in hair follicle initiation, growth, and development.

Analysis of Operation Characteristics of a Diesel Generator Set with Constant‑Power Pulsed Load

Meng Shi,Puyu Wang,Jinquan Wang,Haichao Li,Ye Xu,Kefeng Huang,Jianke Li 대한전기학회 2019 Journal of Electrical Engineering & Technology Vol.14 No.6

To improve the power quality of a diesel generator set with a pulsed load in a microgrid, this paper studied the operation characteristics of the system and proposed pulsed load conditions for an actual project based on test data. For a low average pulse power and a high peak power, a new mathematical model of the pulsed load in diferent operation modes was proposed. Then, a simulation model of the microgrid was established in MATLAB/Simulink, and the operation of the system was studied considering changes in various pulsed load parameters (the peak power Pk, output duty cycle D and switching period Ts). It was verifed by tests that the simulation model is efective and can refect the operation of the system. Based on the simulation model, the operation characteristics of the system with a bivariate pulsed load were studied, and qualifcation conditions for the diesel generator set in an actual project were proposed.

Estimates of Genetic Parameters and Genetic Trends for Production Traits of Inner Mongolian White Cashmere Goat

Bai, Junyan,Zhang, Qin,Li, Jinquan,Dao, Er-Ji,Jia, Xiaoping Asian Australasian Association of Animal Productio 2006 Animal Bioscience Vol.19 No.1

Two different animal models, which differ in whether or not taking maternal genetic effect into account, for estimating genetic parameters of cashmere weight, live body weight, cashmere thickness, staple length, fiber diameter, and fiber length in Inner Mongolia White Cashmere Goat were compared via likelihood ratio test. The results indicate that maternal genetic effect has significant influence on live body weight and cashmere thickness, but no significant influence on the other traits. Using models suitable for each trait, both genetic parameters and trends were analyzed with the MTDFREML program. Heritability estimates from single trait models for cashmere weight, live body weight, cashmere thickness, staple length, fiber diameter and fiber length were found to be 0.30, 0.07, 0.21, 0.29, 0.28 and 0.21, respectively. Genetic correlation estimates from two-trait models between live body weight and all other traits (-0.06~0.07) was negligible, as were those between fiber diameter and all other traits (-0.01~0.03) except cashmere thickness (0.19). Cashmere weight and staple length had moderate to low genetic correlations with other traits (-0.24~0.39 and -0.24~0.34, respectively) except for live body weight and fiber diameter. Cashmere thickness had a strong genetic correlation with fiber length (0.81), and low genetic correlation with other traits (0.19~0.34) except live body weight. Genetic trend analysis suggests that selection for cashmere weight was very effective, which has led to the slow genetic progress of cashmere thickness and fiber length due to their genetic correlations with cashmere weight. The selection for live body weight was not effective, which was consistent with its low inheritability.

Nanostructured gold films for SERS by block copolymer-templated galvanic displacement reactions.

Wang, Yong,Becker, Michael,Wang, Li,Liu, Jinquan,Scholz, Roland,Peng, Juan,Gö,sele, Ulrich,Christiansen, Silke,Kim, Dong Ha,Steinhart, Martin American Chemical Society 2009 Nano letters Vol.9 No.6

<P>Up to now, little effort has been made to exploit large-area high-throughput patterning by block copolymer (BCP) lithography to generate nanostructured substrates with periods well below 100 nm for surface-enhanced Raman scattering (SERS). We show that simple BCP-templated galvanic displacement reactions yield dense arrays of mushroom-shaped gold nanopillars with a period of 50 nm. The nanoporous BCP films used as templates were obtained by swelling-induced reconstruction of reverse micelle monolayers deposited on silicon wafers. Coupling of adjacent mushroom caps almost impinging on each other combined with their strong local curvature results in a high spatial density of hot spots in the narrow gaps between them. Thus, substrates characterized by high SERS efficiencies are obtained.</P>

SNP Discovery from Transcriptome of Cashmere Goat Skin

Wang, Lele,Zhang, Yanjun,Zhao, Meng,Wang, Ruijun,Su, Rui,Li, Jinquan Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.9

The goat Capra hircus is one of several economically important livestock in China. Advances in molecular genetics have led to the identification of several single nucleotide variation markers associated with genes affecting economic traits. Validation of single nucleotide variations in a whole-transcriptome sequencing is critical for understanding the information of molecular genetics. In this paper, we aim to develop a large amount of convinced single nucleotide polymorphisms (SNPs) for Cashmere goat through transcriptome sequencing. In this study, the transcriptomes of Cashmere goat skin at four stages were measured using RNA-sequencing and 90% to 92% unique-mapped-reads were obtained from total-mapped-reads. A total of 56,231 putative SNPs distributed among 10,057 genes were identified. The average minor allele frequency of total SNPs was 18%. GO and KEGG pathway analysis were conducted to analyze the genes containing SNPs. Our follow up biological validation revealed that 64% of SNPs were true SNPs. Our results show that RNA-sequencing is a fast and efficient method for identification of a large number of SNPs. This work provides significant genetic resources for further research on Cashmere goats, especially for the high density linkage map construction and genome-wide association studies.

SNP Discovery from Transcriptome of Cashmere Goat Skin

Lele Wang,Yanjun Zhang,Meng Zhao,Ruijun Wang,Rui Su,Jinquan Li 아세아·태평양축산학회 2015 Animal Bioscience Vol.28 No.9

The goat Capra hircus is one of several economically important livestock in China. Advances in molecular genetics have led to the identification of several single nucleotide variation markers associated with genes affecting economic traits. Validation of single nucleotide variations in a whole-transcriptome sequencing is critical for understanding the information of molecular genetics. In this paper, we aim to develop a large amount of convinced single nucleotide polymorphisms (SNPs) for Cashmere goat through transcriptome sequencing. In this study, the transcriptomes of Cashmere goat skin at four stages were measured using RNA-sequencing and 90% to 92% unique-mapped-reads were obtained from total-mapped-reads. A total of 56,231 putative SNPs distributed among 10,057 genes were identified. The average minor allele frequency of total SNPs was 18%. GO and KEGG pathway analysis were conducted to analyze the genes containing SNPs. Our follow up biological validation revealed that 64% of SNPs were true SNPs. Our results show that RNA-sequencing is a fast and efficient method for identification of a large number of SNPs. This work provides significant genetic resources for further research on Cashmere goats, especially for the high density linkage map construction and genome-wide association studies.