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Shuxiang Zheng,Peilian Wei,Lei Huang,Jin Cai,Zhi-nan Xu 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.2
Glucuronic acid is an important biochemicalwith wide applications in the food and medical industries. The myo-inositol oxygenase (MIOX) gene was synthesizedand expressed in Escherichia coli BL 21(DE3). Afteroptimization of induction conditions and the culturetemperature, the highest MIOX activity (45.46 kU/L) wasachieved when 0.1 mM isopropyl-thio-β-D-galactoside(IPTG) was added to cell cultures with an OD600 value 1.0followed by induction at 26oC for 8 hours. The purifiedMIOX enzyme exerted characteristics similar to the nativeform. Conversion of myo-inositol to glucuronic acid wasperformed using whole cells in a pH 8.5 buffer system. Whole cells harboring myo-inositol oxygenase were usedas a biocatalyst to produce 2.13 g/L of glucuronic acid witha conversion rate of 99%. A promising novel process forglucuronic acid production from abundant agriculturalbyproducts is presented.