Low field magnetic resonance imaging characteristics of experimental canine intracranial hemorrhage

Jeong, Jimo,Jung, Yechan,Jeong, Eunseok,Cho, Youngkwon,Lee, Kichang PAKISTAN VETERINARY JOURNAL 2017 Pakistan veterinary journal Vol.37 No.3

Time-dependent low-field MRI characteristics of canine blood: an in vitro study

Jimo Jeong,Sangjun Park,Eunseok Jeong,Namsoo Kim,Min Su Kim,Yechan Jung,Youngkwon Cho,Kichang Lee 대한수의학회 2016 Journal of Veterinary Science Vol.17 No.1

The present study was conducted to assess time-sensitive magnetic resonance (MR) changes in canine blood using low-field MR. Arterial and venous blood samples were collected from eight healthy beagle dogs. Samples were placed in 5-mL tubes and imaged within 3 hours of collection at 1 day intervals from day 1 to day 30. The following sequences were used: T1-weighted (T1W), T2-weighted (T2W), fluid-attenuated inversion recovery (FLAIR), short tau inversion recovery (STIR), and T2-star gradient-echo (T2*-GRE). Visual comparison of the images revealed that four relatively homogenous blood clots and twelve heterogeneous blood clots developed. The margination of the clot and plasma changed significantly on day 2 and day 13. On day 2, heterogeneous blood clots were differentiated into 2 to 3 signal layers in the T2W, T1W, and especially the STIR images. Hypointense signal layers were also detected in the blood clots in STIR images, which have T2 hypo, FLAIR hypo, and T1 hyper intense signals. In all images, these signal layers remained relatively unchanged until day 13. Overall, the results suggest that hematomas are complex on low-field MRI. Accordingly, it may not be feasible to accurately characterize hemorrhages and predict clot age based on low-field MRI.

General parametric dependence of atmospheric pressure argon plasmas

Jeong Seokyong,Lee Jimo,Yun Gunsu 한국물리학회 2023 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.82 No.1

Using a global model for atmospheric pressure plasma, we investigated general dependence of plasma properties on power density and plasma size. We built a global simulation for a pure argon cylindrical plasma and observed changes in plasma properties with the power density and plasma size. The study of the power dependence shows that the density of excited species is in general proportional to the power when the power density is low, whereas the density becomes saturated when the power density becomes high enough. These trends are explained by a generalized form of particle balance equation, implying that the same trends for reactive species density would emerge in various plasma conditions. For the plasma size dependence, the electron density increases and the electron temperature decreases for increasing plasma size. Both become saturated when the plasma size becomes large enough. These trends of electron density and temperature are explained by the relative change of the difusive loss. Our simulation results give a useful insight into the tendency of plasma properties over a wide range of plasma parameters.

Virtual otoscopy for evaluating the inner ear with a fluid-filled tympanic cavity in dogs

조영권,Jimo Jeong,이해범,김민수,김남수,이기창 대한수의학회 2012 Journal of Veterinary Science Vol.13 No.4

The feasibility of virtual otoscopy (VO) imaging was evaluated in five dogs with experimentally induced otitis media, two control dogs, and two canine patients with otitis media. VO images of the tympanic cavity and ossicles were generated with commercially available software using raw computed tomography (CT) data. Eight out of 10 ears inoculated with pathogen exhibited obvious clinical signs associated with otitis externa. CT images revealed soft tissue density material occupying the tympanic bulla compatible with otitis media in three dogs with experimentally induced otitis media and two patients. No remarkable features were observed on the radiographs. Four different VO views (ear canal, tympanic bulla, eustachian tube, and ossicular chain)were created. Promontory, cochlea window, tympanic, and septum bulla as well as ossicles were easily and clearly distinguished except for the incus and stapes of the clinical patients. VO images were not more suitable than images created with conventional CT for accurately diagnosing otitis media in this study. However, it appears that VO could be more feasible for assessing the complex structure of the inner ear in dogs with fluid-filled tympanic cavities since fluid accumulation within the tympanic bulla did not affect the evaluation of bony tissue in the middle ear on VO images.

Diagnostic imaging features of normal anal sacs in dogs and cats

Yechan Jung,Eunseok Jeong,Sangjun Park,Jimo Jeong,Ul Soo Choi,Min Su Kim,Nam-Soo Kim,Kichang Lee 대한수의학회 2016 Journal of Veterinary Science Vol.17 No.3

This study was conducted to provide normal reference features for canine and feline anal sacs using ultrasound, low-field magnetic resonance imaging (MRI) and radiograph contrast as diagnostic imaging tools. A total of ten clinically normal beagle dogs and eight clinically normally cats were included. General radiography with contrast, ultrasonography and low-field MRI scans were performed. The visualization of anal sacs, which are located at distinct sites in dogs and cats, is possible with a contrast study on radiography. Most surfaces of the anal sacs tissue, occasionally appearing as a hyperechoic thin line, were surrounded by the hypoechoic external sphincter muscle on ultrasonography. The normal anal sac contents of dogs and cats had variable echogenicity. Signals of anal sac contents on low-field MRI varied in cats and dogs, and contrast medium using T1-weighted images enhanced the anal sac walls more obviously than that on ultrasonography. In conclusion, this study provides the normal features of anal sacs from dogs and cats on diagnostic imaging. Further studies including anal sac evaluation are expected to investigate disease conditions.

Repositioning of anti-cancer drug candidate, AZD7762, to an anti-allergic drug suppressing IgE-mediated mast cells and allergic responses via the inhibition of Lyn and Fyn

Park, Young Hwan,Kim, Do-Kyun,Kim, Hyun Woo,Kim, Hyuk Soon,Lee, Dajeong,Lee, Min Bum,Min, Keun Young,Koo, Jimo,Kim, Su Jeong,Kang, Changhee,Kim, Young Mi,Kim, Hyung Sik,Choi, Wahn Soo Elsevier 2018 Biochemical pharmacology Vol.154 No.-

<P><B>Abstract</B></P> <P>Mast cells are critical effector cells in IgE-mediated allergic responses. The aim of this study was to investigate the anti-allergic effects of 3-[(aminocarbonyl)amino]-5-(3-fluorophenyl)-N-(3S)-3-piperidinyl-2-thiophenecarboxamide (AZD7762) in vitro and in vivo. AZD7762 inhibited the antigen-stimulated degranulation from RBL-2H3 (IC<SUB>50</SUB>, ∼27.9 nM) and BMMCs (IC<SUB>50</SUB>, ∼99.3 nM) in a dose-dependent manner. AZD7762 also inhibited the production of TNF-α and IL-4. As the mechanism of its action, AZD7762 inhibited the activation of Syk and its downstream signaling proteins, such as Linker of activated T cells (LAT), phospholipase (PL) Cγ1, Akt, and mitogen-activated protein (MAP) kinases (Erk1/2, p38, and JNK) in mast cells. In in vitro protein kinase assay, AZD7762 inhibited the activity of Lyn and Fyn kinases, which are important for the activation of Syk in mast cells. Furthermore, AZD7762 also suppressed the degranulation of LAD2 human mast cells (IC<SUB>50</SUB>, ∼49.9 nM) and activation of Syk in a dose-dependent manner. As observed in experiments with mast cells in vitro, AZD7762 inhibited antigen-mediated passive cutaneous anaphylaxis in mice (ED<SUB>50</SUB>, ∼35.8 mg/kg). Altogether, these results suggest that AZD7762 could be used as a new therapeutic agent for mast cell-mediated allergic diseases.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Polysaccharide isolated from Aloe vera gel suppresses ovalbumin-induced food allergy through inhibition of Th2 immunity in mice

Lee, Dajeong,Kim, Hyuk Soon,Shin, Eunju,Do, Seon-Gil,Lee, Chong-Kil,Kim, Young Mi,Lee, Min Bum,Min, Keun Young,Koo, Jimo,Kim, Su Jeong,Nam, Seung Taek,Kim, Hyun Woo,Park, Young Hwan,Choi, Wahn Soo Elsevier 2018 Biomedicine & pharmacotherapy Vol.101 No.-

<P><B>Abstract</B></P> <P>An allergic reaction occurs when the immune system overreacts to harmless substance called allergen that gains access to the body. Food allergy is a hypersensitive immune reaction to food proteins and the number of patients with food allergy has recently increased. <I>Aloe Vera</I> is used for wellness and medicinal purposes. In particular, <I>Aloe vera</I> has been reported to enhance immunity. However, the effect of <I>Aloe vera</I> on food allergy is not yet known. In this study, we investigated the effects of processed <I>Aloe vera</I> gel (PAG) containing low molecular weight Aloe polysaccharide (AP) on ovalbumin (OVA)-induced food allergy in mice. Allergic symptoms, rectal temperature, and diarrhea were measured in OVA-induced food allergy mice. Other allergic parameters were also analyzed by RT-PCR, ELISA, flow cytometry, and other biochemical methods. As the results, PAG suppressed the decrease of body temperature, diarrhea, and allergic symptoms in OVA-induced food allergy mice. PAG also reduced serum concentrations of type 2 helper T cell (Th2) cytokines (Interleukin-(IL)-4, IL-5, and IL-13) as well as histamine, mast cell protease-1 (MCP-1), and immunoglobulin (Ig)E. PAG blocked the degranulation of mast cells and infiltration of eosinophils in intestine. Furthermore, PAG suppressed the population of Th2 cells in spleen and mesenteric lymph nodes. PAG also increased the production of IL-10 and population of type 1 regulatory T (Tr1) cells in mice with food allergy. Taken together, our findings suggest that PAG suppressed Th2 immune responses through, at least partially, stimulating the secretion of IL-10 in food allergy mice.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Processed <I>Aloe vera</I> gel (PAG) suppresses food allergy in mice. </LI> <LI> PAG inhibits Th2 immune responses in mice. </LI> <LI> PAG increases the production of IL-10 in vitro and in vivo. </LI> <LI> PAG stimulates the activation of Tr1 cells. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>