Estimation and variable selection for partially functional linear models

Jiang Du,Dengke Xu,Ruiyuan Cao 한국통계학회 2018 Journal of the Korean Statistical Society Vol.47 No.4

In this paper, a new estimation procedure based on composite quantile regression and functional principal component analysis (PCA) method is proposed for the partially functional linear regression models (PFLRMs). The proposed estimation method can simultaneously estimate both the parametric regression coefficients and functional coefficient components without specification of the error distributions. The proposed estimation method is shown to be more efficient empirically for non-normal random error, especially for Cauchy error, and almost as efficient for normal random errors. Furthermore, based on the proposed estimation procedure, we use the penalized composite quantile regression method to study variable selection for parametric part in the PFLRMs. Under certain regularity conditions, consistency, asymptotic normality, and Oracle property of the resulting estimators are derived. Simulation studies and a real data analysis are conducted to assess the finite sample performance of the proposed methods.

Needle Deflection during Insertion into Soft Tissue Based on Virtual Spring Model

Haiyan Du,Yongde Zhang,Jingang Jiang,Yanjiang Zhao 보안공학연구지원센터 2015 International Journal of Multimedia and Ubiquitous Vol.10 No.1

Needle insertion is the most common procedure of minimally invasive interventions. During insertion into a soft tissue, the needle with bevel tip can deflect due to the asymmetric forces acting on the tip of the needle. In this paper, a mechanics-based model is developed to predict the needle deflection. In the model, the needle is considered as a cantilever beam supported by a series of nonlinear springs each of which has stiffness different from each other. The value of stiffness can be calculated by cutting force acting on the needle tip. Based on the model and the analysis of cutting force and friction force, Rayleigh-Ritz method is used to estimate the amount of needle deflection. Experiment shows that the simulation model can accurately predict the deflection of the bevel-tipped needle.

Detection of 881^A→881^G Mutation in Tyrosinase Gene and Associations with the Black Ear Coat Color in Rabbits

Jiang, Y.L.,Fan, X.Z.,Lu, Z.X.,Tang, H.,Xu, J.-Q.,Du, L.-X. Asian Australasian Association of Animal Productio 2002 Animal Bioscience Vol.15 No.10

The tyrosinase gene was selected as a candidate for uncovering genetic mechanism causing 'black ear' coat color in rabbits. A PCR-SSCP detection method was established for the $881^A{\rightarrow}881^G$ mutation located in the central region of the tyrosinase gene between the CuA and CuB binding region signatures, and this was confirmed by sequencing and alignment. Fully consistent associations between the SNP and 'black ear' coat color were observed by analysis in a "black ear" pedigree and on 61 unrelated individuals. This SNP can serve as a molecular marker for use in "back ear" wool rabbit breeding.

Associations of T→A Mutation in the Promoter Region of Myostatin Gene with Birth Weight in Yorkshire Pigs

Jiang, Y-L,Li, N,Fan, X-Z,Xiao, L-R,Xiang, R-L,Hu, X-X,Du, L-X,Wu, C-X Asian Australasian Association of Animal Productio 2002 Animal Bioscience Vol.15 No.11

A T$\longrightarrow$A mutation in the promoter region of porcine myostatin (MSTN) gene has been identified in our previous work. This study analyzed the associations of the myostatin genotypes (TT, TA) caused by this mutation with birth weigh in Yorkshire pigs. Data from 211 unrelated individuals were collected three times from one breeding farm. Detections of the mutation were carried out by PCR-RFLPs approach. The effects of MSTN genotypes (TT and TA) on birth weight were compared by least square means. The results showed that for birth weight of Yorkshire pigs, individuals with TA genotype were significantly higher than those with TT genotype (p<0.05), and the birth weight for pigs with TA genotype were 1.37 kg in average but only 1.25 kg for pigs with TT genotype, indicating a positive effect of birth weight for A allele.

An investigation into the effects of voids, inclusions and minor cracks on major crack propagation by using XFEM

Jiang, Shouyan,Du, Chengbin,Gu, Chongshi Techno-Press 2014 Structural Engineering and Mechanics, An Int'l Jou Vol.49 No.5

For the structures containing multiple discontinuities (voids, inclusions, and cracks), the simulation technologies in the framework of extended finite element method (XFEM) are discussed in details. The level set method is used for representing the location of inner discontinuous interfaces so that the mesh does not need to align with these discontinuities. Several illustrations have been given to verify that the implemented XFEM program is effective. Then, the implemented XFEM program is used to investigate the effects of the voids, inclusions, and minor cracks on the path of major crack propagation. For a plate containing cracks and voids, two possibly crack path can be observed: i) the crack propagates into the void; ii) the crack initially curves towards the void, then, the crack reorients itself and propagates along its original orientation. For a plate with a soft inclusion, the final predicted crack paths tend to close with the inclusion, and an evident difference of crack paths can be observed with different inclusion material properties. However, for a plate with a hard inclusion, the paths tend to away from the inclusion, and a slightly difference of crack paths can only be seen with different inclusion material properties. For a plate with several minor cracks, the trend of crack paths can still be described as that the crack initially curves towards these minor cracks, and then, the crack reorients itself and propagates almost horizontally along its original orientation.

Study on dynamic interaction between crack and inclusion or void by using XFEM

Jiang, Shouyan,Du, Chengbin Techno-Press 2017 Structural Engineering and Mechanics, An Int'l Jou Vol.63 No.3

This paper devoted to study dynamic interaction between crack and inclusion or void by developing the eXtended Finite Element Methods (XFEM). A novel XFEM approximation is presented for these structures containing multi discontinuities (void, inclusion, and crack). The level set methods are used so that elements that include a crack segment, the boundary of a void, or the boundary of an inclusion are not required to conform to discontinuous edges. The investigation covers the effects of a single circular or elliptical void / stiff inclusion, and multi stiff inclusions on the crack propagation path under dynamic loads. Both the void and the inclusion have a significant effect on the dynamic crack propagation path. The crack initially curves towards into the void, then, the crack moves round the void and propagates away the void. If a large void lies in front of crack tip, the crack may propagate into the void. If an enough small void lies in front of crack tip, the void may have a slight or no influence on the crack propagation path. For a stiff inclusion, the crack initially propagates away the inclusion, then, after the crack moves round the inclusion, it starts to propagate along its original path. As ${\delta}$ (the ratio of the elastic modulus of the inclusion to that of the matrix) increases, a larger curvature of the crack path deflection can be observed. However, as ${\delta}$ increases from 2 to 10, the curvature has an evident increase. By comparison, the curvature has a slight increase, as ${\delta}$ increases from 10 to 1000.

Overexpression of cholinergic receptor nicotinic gamma subunit inhibits proliferation and differentiation of bovine preadipocytes

Du Jiawei,Zhao Hui,Song Guibing,Pang Yuan,Jiang Lei,Zan Linsen,Wang Hongbao 아세아·태평양축산학회 2023 Animal Bioscience Vol.36 No.2

Objective: Muscle acetylcholine receptors have five alpha subunits (α, β, δ, ε, or γ), and cholinergic receptor nicotinic gamma subunit (CHRNG) is the γ subunit. It may also play an essential role in biological processes, including cell differentiation, growth, and survival, while the role of CHRNG has not been studied in the literature. Therefore, the purpose of this study is to clarify the effect of CHRNG on the proliferation and differentiation of bovine preadipocytes. Methods: We constructed a CHRNG overexpression adenovirus vector and successfully overexpressed it on bovine preadipocytes. The effects of CHRNG on bovine preadipocyte proliferation were detected by Edu assay, cell counting Kit-8 (CCK-8), real-time fluorescence quantitative polymerase chain reaction (RT-qPCR), Western blot and other techniques. We also performed oil red O, RT-qPCR, Western blot to explore its effect on the differentiation of preadipocytes. Results: The results of Edu proliferation experiments showed that the number of EDUpositive cells in the overexpression group was significantly less. CCK-8 experiments found that the optical density values of the cells in the overexpression group were lower than those of the control group, the mRNA levels of proliferating cell nuclear antigen (PCNA), cyclin A2 (CCNA2), cyclin B1 (CCNB1), cyclin D2 (CCND2) decreased significantly after CHRNG gene overexpression, the mRNA levels of cyclin dependent kinase inhibitor 1A (CDKN1A) increased significantly, and the protein levels of PCNA, CCNB1, CCND2 decreased significantly. Overexpression of CHRNG inhibited the differentiation of bovine preadipocytes. The results of oil red O and triglyceride determination showed that the size and speed of lipid droplets accumulation in the overexpression group were significantly lower. The mRNA and protein levels of peroxisome proliferator activated receptor gamma (PPARγ), CCAAT enhancer binding protein alpha (CEBPα), fatty acid binding protein 4 (FABP4), fatty acid synthase (FASN) decreased significantly. Conclusion: Overexpression of CHRNG in bovine preadipocytes inhibits the proliferation and differentiation of bovine preadipocytes.

Identification of QTLs for seed germination capability after various storage periods using two RIL populations in rice

Jiang, Wenzhu,Lee, Joohyun,Jin, Yong-Mei,Qiao, Yongli,Piao, Rihua,Jang, Sun Mi,Woo, Mi-Ok,Kwon, Soon-Wook,Liu, Xianhu,Pan, Hong-Yu,Du, Xinglin,Koh, Hee-Jong Springer-Verlag 2011 Molecules and cells Vol.31 No.4

Genistein attenuates isoflurane-induced neurotoxicity and improves impaired spatial learning and memory by regulating cAMP/CREB and BDNF-TrkB-PI3K/Akt signaling

Jiang, Tao,Wang, Xiu-qin,Ding, Chuan,Du, Xue-lian The Korean Society of Pharmacology 2017 The Korean Journal of Physiology & Pharmacology Vol.21 No.6

Anesthetics are used extensively in surgeries and related procedures to prevent pain. However, there is some concern regarding neuronal degeneration and cognitive deficits arising from regular anesthetic exposure. Recent studies have indicated that brain-derived neurotrophic factor (BDNF) and cyclic AMP response element-binding protein (CREB) are involved in learning and memory processes. Genistein, a plant-derived isoflavone, has been shown to exhibit neuroprotective effects. The present study was performed to examine the protective effect of genistein against isoflurane-induced neurotoxicity in rats. Neonatal rats were exposed to isoflurane (0.75%, 6 hours) on postnatal day 7 (P7). Separate groups of rat pups were orally administered genistein at doses of 20, 40, or 80 mg/kg body weight from P3 to P15 and then exposed to isoflurane anesthesia on P7. Neuronal apoptosis was detected by TUNEL assay and FluoroJade B staining following isoflurane exposure. Genistein significantly reduced apoptosis in the hippocampus, reduced the expression of proapoptotic factors (Bad, Bax, and cleaved caspase-3), and increased the expression of Bcl-2 and Bcl-xL. RT-PCR analysis revealed enhanced BDNF and TrkB mRNA levels. Genistein effectively upregulated cAMP levels and phosphorylation of CREB and TrkB, leading to activation of cAMP/CREB-BDNF-TrkB signaling. PI3K/Akt signaling was also significantly activated. Genistein administration improved general behavior and enhanced learning and memory in the rats. These observations suggest that genistein exerts neuroprotective effects by suppressing isoflurane-induced neuronal apoptosis and by activating cAMP/CREB-BDNF-TrkB-PI3/Akt signaling.

Synergistic Immunostimulating Activity of Pidotimod and Red Ginseng Acidic Polysaccharide against Cyclophosphamide-induced Immunosuppression

Du, Xiao Fei,Jiang, Cheng Zhe,Wu, Chun Fu,Won, Eun-Kyung,Choung, Se-Young 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.9

We investigated the synergistic effect of combined treatment with red ginseng acidic polysaccharide (RGAP) from Panax ginseng C.A. Meyer and pidotimod in cyclophosphamide-treated mice. The combination of pidotimod and RGAP restored concanavalin A-induced splenic T cell proliferation and LPS-stimulated B cell proliferation significantly. The production of nitric oxide from peritoneal macrophages was increased by the combinations. NK cell activity was increased by RGAP alone or in combination with pidotimod. A synergistic increase in the level of serum IL-12 and interferongamm was observed when the combination of the two was used. RGAP alone or in combination with pidotimod modulated the level of serum C-reactive protein to a near-normal level. These results indicate that combinations of pidotimod and RGAP are synergistic and suggest that combination therapy using pidotimod and RGAP for improving immune activity may provide an additional benefit over the use of the two drugs by themselves.