Cloning of porcine chemerin, ChemR23 and GPR1 and their involvement in regulation of Lipogenesis

( Jianfeng Huang ),( Jian Zhang ),( Ting Lei ),( Xiaodong Chen ),( Yan Zhang ),( Lulu Zhou ),( An Yu ),( Zhilong Chen ),( Ronghua Zhou ),( Zaiqing Yang ) 생화학분자생물학회 (구 한국생화학분자생물학회) 2010 BMB Reports Vol.43 No.7

Chemerin is a novel adipokine which is abundant in adipose tissue to promote adipocyte differentiation and with significant relativity to BMI and insulin sensitivity. We report here the molecular characterization of porcine chemerin and its receptors ChemR23 and GPR1, as well as their transcriptional regulation during lipogenesis. Chemerin was mainly expressed in liver, intestine, kidney and adipose tissue, consistent with the expression pattern of GPR1, but not ChemR23, which was predominantly present in spleen and temperately in adipose tissue. We further investigated the lipogenesis-related transcriptional activation of PPARγ and KLF15 on chemerin and its receptors. The data showed that KLF15, but not PPARγ, can up-regulate the mRNA level of chemerin, ChemR23 and GPR1, which was consistent with the results of luciferase assay that confirmed the effect of KLF15 on ChemR23 promoter. Taken together, our data provide basic molecular information for the further investigation on the function of chemerin in lipogenesis. [BMB reports 2010; 43(7): 491-498]

Phase lag compensation for improving the stability of LCL‑type converters under weak grid condition

Jianfeng Wang,Guobing Pan,Jing Ouyang,Chengyao Liu,Yinghao Zhou,Gong Fei 전력전자학회 2022 JOURNAL OF POWER ELECTRONICS Vol.22 No.5

LCL-type converters are widely used in the sustainable energy generation system due to their flexible current control strategies and high efficiency. The LCL filter has a resonance peak, which needs to be handled appropriately; otherwise, it causes system instability. Single-loop feedback control strategy is very popular at present; it does not require additional sensors to measure the state variables for active damping. Under weak grid condition, the resonance peak of LCL filter shifts to the left evidently and greatly reduces the stability margin; thus, maintaining the stability of single-loop control is a challenging task. This paper presents an improved grid current feedback (GCF) single-loop control strategy, which is designed to raise the stability margin of system to adapt weak grid condition. Low pass filter controller is used for phase lag compensation. A phase margin design method is proposed to ensure system stability, and the corresponding discrete method in z-domain has been proposed in detail. Experiments performed on a three-phase converter platform are finally presented to verify the effectiveness of the proposed phase lag compensation for GCF control strategy.

Tissue-Specific CRISPR/Cas9 System of Cotton Pollen with GhPLIMP2b and GhMYB24 Promoters

Lei Jianfeng,Dai Peihong,Li Jiyang,Yang Mi,Li Xiuqin,Zhang Wanqi,Zhou Guantong,WangzhenGuo,Liu Xiaodong 한국식물학회 2021 Journal of Plant Biology Vol.64 No.1

CRISPR/Cas9 technology is a powerful tool for improving crop genetic traits. However, CRISPR/Cas9 system for efficient expression of cotton germ cells has still not been established. In this study, we developed a tissue-specific vectors to drive Cas9 expression with GhPLIMP2b and GhMYB24 promoters and established the effective method to transform cotton pollen by Agrobacterium vacuum infiltration. GhPLIMP2b and GhMYB24 promoters of cotton pollen were cloned into Cas9 expression vectors. The sgRNAs targetting to CLA1, ERA1 and GGB (drought-resistant negative regulation genes) were designed and constructed into GhPLIMP2b and GhMYB24 promoter vectors. Cotton pollens were tranformed by Agrobacterium vacuum infiltration with GhPLIMP2b and GhMYB24 promoter vectors. The results of clone sequencing shown that mutation types of the sequence were mainly base substitutions wth the frequency from 3.29 to 6.45%. Eleven potential off-target sites were chosen and two sites were observed. Our results indicated that GhPLIM2bP::Cas9 and GhMYB24P::Cas9 editing vectors achieved targeted edition of endogenous genes in cotton pollen, but there were a few off-target effects. This study provides an effective gene-editing system for the rapid acquisition of cotton mutants using pollen as a transgenic receptor.

Gear fault diagnosis under variable conditions with intrinsic time-scale decomposition-singular value decomposition and support vector machine

Zhanqiang Xing,Jianfeng Qu,Yi Chai,Qiu Tang,Yuming Zhou 대한기계학회 2017 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.31 No.2

The gear vibration signal is nonlinear and non-stationary, gear fault diagnosis under variable conditions has always been unsatisfactory. To solve this problem, an intelligent fault diagnosis method based on Intrinsic time-scale decomposition (ITD)-Singular value decomposition (SVD) and Support vector machine (SVM) is proposed in this paper. The ITD method is adopted to decompose the vibration signal of gearbox into several Proper rotation components (PRCs). Subsequently, the singular value decomposition is proposed to obtain the singular value vectors of the proper rotation components and improve the robustness of feature extraction under variable conditions. Finally, the Support vector machine is applied to classify the fault type of gear. According to the experimental results, the performance of ITD-SVD exceeds those of the time-frequency analysis methods with EMD and WPT combined with SVD for feature extraction, and the classifier of SVM outperforms those for K-nearest neighbors (K-NN) and Back propagation (BP). Moreover, the proposed approach can accurately diagnose and identify different fault types of gear under variable conditions.

Preparation of cationic functional polymer poly(Acryloxyethyltrimethyl ammonium chloride)/SiO2 and its adsorption characteristics for heparin

Jiying Men,Jianfeng Guo,Weihong Zhou,Nanyan Dong,Xilun Pang,Baojiao Gao 한국화학공학회 2017 Korean Journal of Chemical Engineering Vol.34 No.7

Ion-exchange is a widely used purification technology in the heparin manufacturing process. To improve the rate and efficiency, the cationic monomer Acryloxyethyltrimethyl ammonium chloride (DAC) was grafted on silica gel particles by using a surface-initiated graft-polymerization technique, and a novel adsorption polymer of PDAC/SiO2 was prepared. The adsorption experiments of PDAC/SiO2 for heparin show that there are strong electrostatic interactions between heparin with a high density of negative charges and PDAC/SiO2 that exists in many quaternary ammonium cations. Comparing the adsorption kinetics of PDAC/SiO2 with D201 anionic strong alkali exchange resin, PDAC/SiO2 shows higher adsorption capacity and faster adsorption rate. The pH and temperature of solutions have a great influence on the adsorption amount, and there is a maximum adsorption capacity of 121mg/g when pH=7 at 25 oC. The adsorption of PDAC/SiO2 for heparin is an exothermic process and driven by entropy.

A Novel Fixed Twin-Rotor Unmanned Aerial Vehicle with Variable Angle Louver Rudder

Yixin Zhang,Jianfeng Zhou,Shaoping Wang,Mengqi Yang,Shaoshi Li 제어로봇시스템학회 2021 제어로봇시스템학회 국제학술대회 논문집 Vol.2021 No.10

A novel fixed twin-rotor unmanned aerial vehicle (UAV) based on the variable angle louver rudder (VALR) is developed in this work. In view of the mechanical characteristics under the action of the louver rudder, specific kinematics and dynamics models are derived. The aerodynamic characteristics of the UAV are studied through computational fluid dynamics simulation, and a ground measurement system is designed to analyze the aerodynamic characteristics of the prototype. An attitude control model is proposed, and maneuvers such as take-off, landing, hovering, and braking are realized through flight experiments, which verify the feasibility of the prototype design. This research shows that the new layout with the VALR system, combined with the advantages of rudder control and vector control, can reduce the structural complexity and deadweight of the UAV while ensuring flight stability and improving maneuverability. Moreover, the novel UAV has higher energy efficiency and economy.

Laparoscopic surgery contributes to a decrease in short-term complications in surgical ulcerative colitis patients during 2008–2017: a multicenter retrospective study in China

Zerong Cai,Xiaosheng He,Jianfeng Gong,Peng Du,Wenjian Meng,Wei Zhou,Jinbo Jiang,Bin Wu,Weitang Yuan,Qi Xue,Lianwen Yuan,Jinhai Wang,Jiandong Tai,Jie Liang,Weiming Zhu,Ping Lan,Xiaojian Wu 대한장연구학회 2023 Intestinal Research Vol.21 No.2

Background/Aims: The aim of this study was to analyze the chronological changes in postoperative complications in surgical ulcerative colitis patients over the past decade in China and to investigate the potential parameters that contributed to the changes. Methods: Ulcerative colitis patients who underwent surgery during 2008–2017 were retrospectively enrolled from 13 hospitals in China. Postoperative complications were compared among different operation years. Risk factors for complications were identified by logistic regression analysis. Results: A total of 446 surgical ulcerative colitis patients were analyzed. Fewer short-term complications (24.8% vs. 41.0%, P=0.001) and more laparoscopic surgeries (66.4% vs. 25.0%, P<0.001) were found among patients who received surgery during 2014–2017 than 2008–2013. Logistic regression suggested that independent protective factors against short-term complications were a higher preoperative body mass index (odds ratio [OR], 0.870; 95% confidence interval [CI], 0.785–0.964; P=0.008), laparoscopic surgery (OR, 0.391; 95% CI, 0.217–0.705; P=0.002) and elective surgery (OR, 0.213; 95% CI, 0.067–0.675; P=0.009). The chronological decrease in short-term complications was associated with an increase in laparoscopic surgery. Conclusions: Our data revealed a downward trend of short-term postoperative complications among surgical ulcerative colitis patients in China during the past decade, which may be due to the promotion of minimally invasive techniques among Chinese surgeons.

Study of the Effectiveness of the RVEs for Random Short Fiber Reinforced Elastomer Composites

Lili Chen,Boqin Gu,Jianfeng Zhou,Jiahui Tao 한국섬유공학회 2019 Fibers and polymers Vol.20 No.7

The effectiveness of the representative volume elements (RVEs) established by a modified random sequentialadsorption method for random short fiber reinforced elastomer composites (SFECs) was studied. The RVE is considered tobe effective when the RVE’s fiber orientation is isotropic. And the effectiveness of the RVEs was verified by evaluating themechanical properties in different loading directions based on the finite element method. The results show that the fibernumber N=4000 can be regarded as a steady threshold for the isotropy of the fiber orientation in RVEs with various fiberaspect ratios and dilute volume fraction.

The effect of Glomus intraradices on the physiological properties of Panax ginseng and on rhizospheric microbial diversity

Lei Tian,Shaohua Shi,Lina Ma,Xue Zhou,Shasha Luo,Jianfeng Zhang,Baohui Lu,Chunjie Tian 고려인삼학회 2019 Journal of Ginseng Research Vol.43 No.1

Background: Glomus intraradices is a species of arbuscular mycorrhizal fungi that, as an obligate endomycorrhiza, can form mutually beneficial associations with plants. Panax ginseng is a popular traditional Chinese medicine; however, problems associated with ginseng planting, such as pesticide residues, reduce the ginseng quality. Methods: In this experiment, we studied the effect of inoculating G. intraradices on several physiological properties and microbial communities of ginseng. UV-Visible Spectrum method was used to detect physical properties. Denaturing gradient gel electrophoresis method was used to analyze microbial communities. Results: The results indicated that inoculation with G. intraradices can improve the colonization rate of lateral ginseng roots, increase the levels of monomeric and total ginsenosides, and improve root activity as well as polyphenol oxidase and catalase activities. We also studied the bacterial and fungal communities in ginseng rhizospheric soil. In our study, G. intraradices inoculation improved the abundance and Shannon diversity of bacteria, whereas fungi showed a reciprocal effect. Furthermore, we found that G. intraradices inoculation might increase some beneficial bacterial species and decreased pathogenic fungi in rhizospheric soil of ginseng. Conclusion: Our results showed that G. intraradices can benefit ginseng planting which may have some instructive and practical significance for planting ginseng in farmland.

The effect of Glomus intraradices on the physiological properties of Panax ginseng and on rhizospheric microbial diversity

Tian, Lei,Shi, Shaohua,Ma, Lina,Zhou, Xue,Luo, Shasha,Zhang, Jianfeng,Lu, Baohui,Tian, Chunjie The Korean Society of Ginseng 2019 Journal of Ginseng Research Vol.43 No.1

Background: Glomus intraradices is a species of arbuscular mycorrhizal fungi that, as an obligate endomycorrhiza, can form mutually beneficial associations with plants. Panax ginseng is a popular traditional Chinese medicine; however, problems associated with ginseng planting, such as pesticide residues, reduce the ginseng quality. Methods: In this experiment, we studied the effect of inoculating G. intraradices on several physiological properties and microbial communities of ginseng. UV-Visible Spectrum method was used to detect physical properties. Denaturing gradient gel electrophoresis method was used to analyze microbial communities. Results: The results indicated that inoculation with G. intraradices can improve the colonization rate of lateral ginseng roots, increase the levels of monomeric and total ginsenosides, and improve root activity as well as polyphenol oxidase and catalase activities. We also studied the bacterial and fungal communities in ginseng rhizospheric soil. In our study, G. intraradices inoculation improved the abundance and Shannon diversity of bacteria, whereas fungi showed a reciprocal effect. Furthermore, we found that G. intraradices inoculation might increase some beneficial bacterial species and decreased pathogenic fungi in rhizospheric soil of ginseng. Conclusion: Our results showed that G. intraradices can benefit ginseng planting which may have some instructive and practical significance for planting ginseng in farmland.