Proteomic Analysis between U87MG and U343MG-A Cell Lines: Searching for Candidate Proteins for Glioma Invasion

Jian Pei,Kyung-Sub Moon,SangO Pan,Kyung-Hwa Lee,Hyang-Hwa Ryu,Tae-Young Jung,In-Young Kim,Woo-Yeol Jang,Chae-Hun Jung,Shin Jung 대한뇌종양학회 2014 Brain Tumor Research and Treatment Vol.2 No.1

Background To investigate the molecular basis for invasion of malignant gliomas, proteomic analysis approach was carried out using two human glioma cell lines, U87MG and U343MG-A that demonstrate different motility and invasiveness in in vitro experiments. Methods High-resolution two-dimensional gel electrophoresis and matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry analysis were performed. Results Nine distinct protein spots that were recognized with significant alteration between the two cell lines. Five of these protein spots were up-regulated in U87MG and four were up-regulated in U343MG-A. Conclusion Among these proteins, cathepsin D was shown to be one of the important proteins which are related with glioma invasion. However, further studies are necessary to reveal the exact role and mechanism of cathepsin D in glioma invasion.

A new recombined porcine reproductive and respiratory syndrome virus virulent strain in China

Jian-guo Dong,Lin-yang Yu,Pei-pei Wang,Le-yi Zhang,Yan-ling Liu,Peng-shuai Liang,Chang-xu Song 대한수의학회 2018 Journal of Veterinary Science Vol.19 No.1

Porcine reproductive and respiratory syndrome (PRRS) is one of the most important swine diseases worldwide. In the present study, a new virulent strain of PRRS virus (PRRSV), GDsg, was isolated in Guangdong province, China, and caused high fever, high morbidity, and high mortality in sows and piglets. The genome of this new strain was 15,413 nucleotides (nt) long, and comparative analysis revealed that GDsg shared 82.4% to 94% identity with type 2 PRRSV strains, but only 61.5% identity with type 1 PRRSV Lelystad virus strain. Phylogenetic analysis indicated that type 2 PRRSV isolates include five subgenotypes (I, II, III, IV, and V), which are represented by NADC30, VR-2332, GM2, CH-1a, and HuN4, respectively. Moreover, GDsg belongs to a newly emerging type 2 PRRSV subgenotype III. More interestingly, the newly isolated GDsg strain has multiple discontinuous nt deletions, 131 (19 + 18 + 94) at position 1404–1540 and a 107 nt insertion in the NSP2 region. Most importantly, the GDsg strain was identified as a virus recombined between low pathogenic field strain QYYZ and vaccine strain JXA1-P80. In conclusion, a new independent subgenotype and recombinant PRRSV strain has emerged in China and could be a new threat to the swine industry of China.

Anti-tumor Activity and Apoptosis-regulation Mechanisms of Bufalin in Various Cancers: New Hope for Cancer Patients

Yin, Pei-Hao,Liu, Xuan,Qiu, Yan-Yan,Cai, Jian-Feng,Qin, Jian-Min,Zhu, Hui-Rong,Li, Qi Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.11

The induction of apoptosis in target cells is a key mechanism for most anti-tumor therapies. Bufalin is a cardiotonic steroid that has the potential to induce differentiation and apoptosis of tumor cells. Research on bufalin has so far mainly involved leukemia, prostate cancer, gastric cancer and liver cancer, and has been confined to in vitro studies. The bufadienolides bufalin and cinobufagin have been shown to induce apoptosis in a wide spectrum of cancer cell. The present article reviews the anticancer effects of bufalin. It induces apoptosis of lung cancer cells via the PI3K/Akt pathway and also suppressed the proliferation of human non-small cell lung cancer A549 cell line in a time and dose dependent manner. Bufalin, bufotalin and gamabufotalin, key bufadienolides, significantly sensitize human breast cancer cells with differing ER-alpha status to apoptosis induction by the TNF-related apoptosis-inducing ligand (TRAIL). In addition, bufadienolides induce prostate cancer cell apoptosis more significantly than that in breast epithelial cell lines. Similar effects have been observed with hepatocellular carcinoma (HCC) but the detailed molecular mechanisms of inducing apoptosis in this case are still unclear. Bufalin exerts profound effects on leukemia therapy in vitro. Results of multiple studies indicate that bufalin has marked anti-tumor activities through its ability to induce apoptosis. Large-scale randomized, double-blind, placebo or positive drug parallel controlled studies are now required to confirm the efficacy and apoptosis-inducing potential of bufalin in various cancers in the cliniucal setting.

Dong Hai Bridge Sea-Crossing Project in Shanghai, China : General Concept, Design, Construction and Durability 설계, 시공, 내구성 및 일반사항

Lin, Yuan-Pei,Zhang, Zeng-huan,Tang, Wei,Lu, Yong-Cheng,Ding, Jian-Kang,Zhang, Jian-Ying Korean Group of IABSE;Korea wind Engineering Resea 2006 Proceedings of the International Symposium on Sea- Vol.2006 No.-

Dong Hai Bridge in Shanghai is the longest in world and the first sea-crossing project in china, the total length of which is 32.5km and the width of the bridge is 31.5m. In this paper the general concepts of design, construction and durability are introduced. 상하이의 동해대교는 전장 32.5km 폭 31.5m 의 세계 최장 교량이며, 중국에서는 최초의 해상 횡단 프로젝트이다. 이논문에서는 동해대교의 설계, 건설, 그리고 내구성에 대한 기본 개념을 소개한다.

Design and Analysis of Spider Bionic Flow Field for Proton Exchange Membrane Fuel Cell

Jian Yao,Fayi Ya,Xuejian Pei The Korean Electrochemical Society 2023 Journal of electrochemical science and technology Vol.14 No.1

Proton exchange membrane fuel cell (PEMFC) is a portable and clean power generation device. The structural arrangement of the flow field has a significant influence on the delivery efficiency of PEMFC. In this article, a new bionic flow channel is designed based on the inspiration of a spider shape. The branch channel width and branch corner are studied as the focus, and its simulation is carried out by the method of computational fluid dynamics (CFD). The results show that when channel width/rib width and corner of the branch are 1.5 and 130° , respectively, it is the best numerical combination and the cell comprehensive performance is excellent. The final model using this numerical combination is compared with the traditional flow channel model to verify the advancement of this scheme.

An Effective Guidewire Looping Technique for the Recanalization of Occlusive Segments of Infrapopliteal Vessels

Jian-bo Wang,Jun-gong Zhao,Ming-hua Li,Yue-qi Zhu,Jue Wang,Pei-lei Zhang 대한영상의학회 2010 Korean Journal of Radiology Vol.11 No.4

Objective: To determine the efficacy, safety and primary follow-up results of a guidewire looping technique for the treatment of infrapopliteal arteries. Materials and Methods: From October 2006 to May 2008, an intraluminal angioplasty of the infrapopliteal arteries was attempted in 200 consecutive patients. Altogether, 417 infrapopliteal lesions, with lengths varying from 2 cm to 32 cm, were treated as part of this study, including 305 lesions in the anterior tibial arteries, 89 in the posterior tibial arteries, and 23 in the peroneal arteries. The ‘U’-shaped guidewire technique was attempted in 393 lesions from 361 limbs. The tip of a hydrophilic 0.035-inch guidewire was formed into a ‘U’ shape with the aid of a 4-Fr catheter and collateral branch vessel to recanalize the completely occluded long segment lesions. Results: A successful angioplasty with at least one artery recanalized directly to the malleolar or dorsal foot was achieved in 322 limbs (89%). The looping technique had a success rate of 90% (352 of 393 lesions). After the procedure, the rest pain was relieved in 58 of 69 patients, while 207 of 245 limbs (85%) showed improvement for intermittent claudication. Complete wound healing was noted in 21 of 54 patients, while 20 of 54 patients showed an improvement in the wound size or depth. A total of 38 major immediate procedure-related complications were noted, including retroperitoneal hematoma, distal emboli, and vessel rupture. Conclusion: The results of this study suggests that the guidewire looping technique is a safe and effective method for the recanalization of the occluded lesions in infrapopliteal vessels. Objective: To determine the efficacy, safety and primary follow-up results of a guidewire looping technique for the treatment of infrapopliteal arteries. Materials and Methods: From October 2006 to May 2008, an intraluminal angioplasty of the infrapopliteal arteries was attempted in 200 consecutive patients. Altogether, 417 infrapopliteal lesions, with lengths varying from 2 cm to 32 cm, were treated as part of this study, including 305 lesions in the anterior tibial arteries, 89 in the posterior tibial arteries, and 23 in the peroneal arteries. The ‘U’-shaped guidewire technique was attempted in 393 lesions from 361 limbs. The tip of a hydrophilic 0.035-inch guidewire was formed into a ‘U’ shape with the aid of a 4-Fr catheter and collateral branch vessel to recanalize the completely occluded long segment lesions. Results: A successful angioplasty with at least one artery recanalized directly to the malleolar or dorsal foot was achieved in 322 limbs (89%). The looping technique had a success rate of 90% (352 of 393 lesions). After the procedure, the rest pain was relieved in 58 of 69 patients, while 207 of 245 limbs (85%) showed improvement for intermittent claudication. Complete wound healing was noted in 21 of 54 patients, while 20 of 54 patients showed an improvement in the wound size or depth. A total of 38 major immediate procedure-related complications were noted, including retroperitoneal hematoma, distal emboli, and vessel rupture. Conclusion: The results of this study suggests that the guidewire looping technique is a safe and effective method for the recanalization of the occluded lesions in infrapopliteal vessels.

Alterations of Amino Acid Level in Depressed Rat Brain

Pei Yang,Xuechun Li,Jian Ni,Jingchen Tian,Fu Jing,Changhai Qu,Longfei Lin,Hui Zhang 대한약리학회 2014 The Korean Journal of Physiology & Pharmacology Vol.18 No.5

Amino-acid neurotransmitter system dysfunction plays a major role in the pathophysiology ofdepression. Several studies have demonstrated the potential of amino acids as a source of neuro-specificbiomarkers could be used in future diagnosis of depression. Only partial amino acids such as glycineand asparagine were determined from certain parts of rats’ brain included hippocampi and cerebralcortex in previous studies. However, according to systematic biology, amino acids in different areaof brain are interacted and interrelated. Hence, the determination of 34 amino acids through entirerats’ brain was conducted in this study in order to demonstrate more possibilities for biomarkers ofdepression by discovering other potential amino acids in more areas of rats’ brain. As a result, 4 aminoacids (L-aspartic acid, L-glutamine, taurine and γ -amino-n-butyric acid) among 34 were typicallyidentified as potentially primary biomarkers of depression by data statistics. Meanwhile, anantidepressant called Fluoxetine was employed to verify other potential amino acids which were notidentified by data statistics. Eventually, we found L-α -amino-adipic acid could also become a newpotentially secondary biomarker of depression after drug validation. In conclusion, we suggested thatL-aspartic acid, L-glutamine, taurine, γ-amino-n-butyric acid and L-α-amino-adipic acid might becomepotential biomarkers for future diagnosis of depression and development of antidepressant.

Alterations of Amino Acid Level in Depressed Rat Brain

Yang, Pei,Li, Xuechun,Ni, Jian,Tian, Jingchen,Jing, Fu,Qu, Changhai,Lin, Longfei,Zhang, Hui The Korean Society of Pharmacology 2014 The Korean Journal of Physiology & Pharmacology Vol.18 No.5

Amino-acid neurotransmitter system dysfunction plays a major role in the pathophysiology of depression. Several studies have demonstrated the potential of amino acids as a source of neuro-specific biomarkers could be used in future diagnosis of depression. Only partial amino acids such as glycine and asparagine were determined from certain parts of rats' brain included hippocampi and cerebral cortex in previous studies. However, according to systematic biology, amino acids in different area of brain are interacted and interrelated. Hence, the determination of 34 amino acids through entire rats' brain was conducted in this study in order to demonstrate more possibilities for biomarkers of depression by discovering other potential amino acids in more areas of rats' brain. As a result, 4 amino acids (L-aspartic acid, L-glutamine, taurine and ${\gamma}$-amino-n-butyric acid) among 34 were typically identified as potentially primary biomarkers of depression by data statistics. Meanwhile, an antidepressant called Fluoxetine was employed to verify other potential amino acids which were not identified by data statistics. Eventually, we found L-${\alpha}$-amino-adipic acid could also become a new potentially secondary biomarker of depression after drug validation. In conclusion, we suggested that L-aspartic acid, L-glutamine, taurine, ${\gamma}$-amino-n-butyric acid and L-${\alpha}$-amino-adipic acid might become potential biomarkers for future diagnosis of depression and development of antidepressant.

Enhancing membrane wetting resistance through superhydrophobic modification by polydimethylsilane-grafted-SiO2 nanoparticles

Moau Jian Toh,Pei Ching Oh,Abdul Latif Ahmada,Julien Caille 한국화학공학회 2019 Korean Journal of Chemical Engineering Vol.36 No.11

Membrane gas-liquid separation technology has been widely employed in membrane filtration, distillation, and gas absorption, attributed to its high mass transfer efficiency. However, hydrophobic membranes may suffer from pore wetting at low operational pressure difference, leading to the deterioration of removal flux. Hence, anti-wetting strategy via membrane surface modification to improve its intrinsic hydrophobicity needs to be investigated. In this work, modified superhydrophobic polyvinylidene fluoride-co-hexafluoropropylene (PVDF-HFP) membrane was synthesized via non-solvent induced phase separation. Polydimethylsilane-grafted-silica (PGS) nanoparticles with non-polar Si-O-Si bonds were used as surface modifier in coagulation bath to enhance membrane surface hydrophobicity. Results demonstrated that the addition of nanoparticles improved the surface roughness via formation of hierarchical structure. Additionally, the deposition of nanoparticles on polymer spherulites significantly reduced the surface free energy. As a result, modified membranes achieved superhydrophobicity with water contact angle exceeding 150o. The stability tests also showed that the deposition layer of modified membrane was mechanically and thermally robust. This superhydrophobic modification by PGS nanoparticles is an advanced and facile approach to alleviate membrane wetting.

Fluid-structure coupling effects on periodically transient flow of a single-blade sewage centrifugal pump

Ji Pei,Shouqi Yuan,Jian-Ping Yuan 대한기계학회 2013 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.27 No.7

A partitioned fluid-structure interaction (FSI) solving strategy that depends on problem characteristics is applied to quantitatively obtain the coupling effects of a fluid-structure system in a single-blade centrifugal pump on the unsteady flow. A two-way coupling method is employed to realize strong FSI effects in the calculation procedure. The successful impeller oscillation measurement using two proximity sensors validated the FSI simulation accuracy in a complicated and practical fluid-structure system having a rotating component. The results show that the hydrodynamic force deviation can be observed in the results for the coupled versus uncoupled cases. Additionally,the coupled unsteady pressure is larger than the uncoupled value for every monitoring point at every impeller rotation position. Comparison results for different monitoring points under an overload condition and partial-load condition display the same regularities. To some extent, this interaction mechanism would affect the accuracy and reliability of the unsteady flow and rotor deflection analysis.