Low Molecular Weight Polyethylenimine-Mitochondrial Leader Peptide Conjugate for DNA Delivery to Mitochondria

Choi, Joon-Sig,Choi, Min-Ji,Go, Gyeong-Su,Rhee, Byoung-Doo,KimPak, Young-Mi,Bang, In-Seok,Lee, Min-Hyung Korean Chemical Society 2006 Bulletin of the Korean Chemical Society Vol.27 No.9

It has been found that a number of diseases are associated with mutations in the mitochondrial DNA. Therapeutic gene delivery to mitochondria has been suggested as a clinical option for these diseases. In this study, we developed a gene carrier to mitochondria by the conjugation of mitochondrial leader peptide (LP) to polyethylenimine (PEI). Mitochondrial LP conjugated PEI (PEI-LP) was synthesized with low molecular weight PEI (2,000 Da, PEI2K). Gel retardation assay showed that PEI2K-LP formed complexes at a 1.0/1 weight ratio. In addition, PEI2K-LP protected DNA from the enzymatic degradation for at least 60 min, while naked DNA was completely degraded within 20 min. PEI2K-LP was compared with LP conjugated high molecular weight PEI (25,000 Da, PEI25K) in terms of toxicity and delivery efficiency. MTT assay showed that PEI2K-LP had much lower cytotoxicity than PEI25K-LP to 293 cells. In addition, cell-free DNA delivery assay showed that PEI2K-LP delivered more DNA to mitochondria at a 1.8/1 weight ratio than naked DNA or PEI. This result suggests that PEI2K-LP may be useful for the development of mitochondrial gene therapy system with lower cytotoxicity.

상악 구개측 중앙부에 매복된 과잉치

민성진,최형준,,김성오,이제호,곽지윤,최병재 大韓小兒齒科學會 2005 大韓小兒齒科學會誌 Vol.32 No.4

과잉치는 치배 형성기에 정상 치판의 과도한 증식의 결과로 발생하며 산발적일 수도 있고 유전적일 수도 있다. 발생 빈도는 1~3%이며,2:1의 비율로 남아에게 호발한다. 주로 상악 전방부에서 많이 발견되며, 매복, 영구치의 위치 이상, 정중부 이개, 낭종, 비강으로의 맹출 등과 같은 다양한 합병증을 나타낼 수도 있다. 상악 전방부에서 발견되어지는 과잉치는 치축방향에 따라 위치가 결정되며 상악 전방부 과잉치의 1/3 정도는 역위 매복되어 맹출의 가능성이 없지만, 치낭의 치관 부위가 정상적인 관계로 존재하는 한 과잉치의 이동이 일어날 수 있다. 치아의 이동은 경구개, 소구치 부위, 비강까지도 일어날 수 있다. 또한 전상악 수직고경의 성장으로 시간이 지남에 라 외과적 접근이 어려워질 수 있다. 이에 본 두 증례에서는 역위 매복된 상악 전방부의 과잉치가 구개 측 중앙부까지 이동된 것을 발견하여 외과적으로 제거하였다. Supernumerary tooth develops as a result of abnormal proliferation of the dental lamina during the initiation stage of dental development. It could be a sporadic occurrence or a hereditary transmission. Supernumerary tooth occurs with a frequency of 1 to 3%, Generally, there is a 2:1 preference for boys. It is usually found in the anterior portion of the maxilla and may be associated with complications such as impaction, malposition of permanent teeth, formation of diastema, cysts and eruption into nasal cavity. The position of supernumerary tooth found in the anterior portion of the maxilla is determined by the axis of the tooth. One third of supernumerary tooth in the anterior portion of the maxilla has no possibility of eruption due to its invertedly impacted position. However, as long as the coronal part of the follicle remain intact, migration of supernumerary tooth is possible. The migration may occur into the palate, the premolar region, the sinus or the nasal cavity. Also, growth of vertical dimension of maxilla could make surgical approach difficult as time goes by. For this reason, we have found invertedly impacted mesiodens moved to the vault of the palate in the two cases, and extracted supernumerary tooth surgically.

해안 잠재 친수여가공간 개발대상지에 대한 사례연구

민병형,이정환,정지윤 동아대학교 해양자원연구소 2000 硏究論文集 Vol.13 No.1

A study will be exalted of live quality that to use of Sea Front for new digging and development in the conceal of Sea Front as used always for live space. This summarize shows the following result. 1.Busan of decide to befroe time-table to development that seven area selected in the conceal of Sea Front to position of the Young Do , SeuㆍSahagu. 2.This seven area withen , A exercise ground area of a Dong sam don-Young Do of the study to development forms. As the proposed first case and second case to aid first case to will be canvass for superior to aid of SeaㆍCure resort 3.This seven area withen, a excrise ground area of Dong sam dong-Young Do Gu the study to development forms As the proposed first case and second case and first case to will be canvass for superior to aid of SeaㆍCure resort 4.A exercise ground area Dong sam dong - Young Do to aid of SeaㆍCure resort that through SeaㆍCure culture to offer, we will be again the SeaㆍSight-seeing city to used the SeaㆍSight - seeing. 5.SeaㆍCure resort area to aid of in the development plans to very suit for used a place and space to disposition, plans to disposition of institute objects for odd used.

人蔘의 吸濕 平衡含水率

崔秉旻,朴又龍,洪志亨 順天大學校 農業科學硏究所 1992 農業科學硏究 Vol.6 No.-

Adsorption equilibrium moisture content of Peeled, Unpeeled and Taegeuk ginseng was obtained to provide essential data to design storage and drying systems for ginseng by the static method with four levels of temperature(20℃, 30℃, 40℃, 50℃) and five levels of relative humidity from 31% toe 88%. Also, empirical equilibrium moisture content model was developed for users to use easily. Performance of five selected equilibrium moisture content models and the empirical model were fitted with experimental equilibrium moisture content data. Oswin, Henderson and the empirical models showed best fit.

3차원 사이버 스페이스의 VR(가상현실)의 활용

한민형,이지은 용인대학교 조형연구소 2000 조형논총 Vol.5 No.-

Virtual Reality, which is closely related to our daily lives is not only based on imagination anymore. Still, there are mere definite notion and vague substantiality. It is only matter of time for the vitual reality to develope rapidly with our lives. Cyber space is not a two-dimensional, but a three-dimensional space connected with network. We need computers and all sorts of other equipment to great this space. But this caber space is an untouchable imaginary world. In short, our exchanging e-mails with friends are all included in the virtual reality. It is one of the activities of virtual reality which is realized in the unsubstantial space and not through conversation in person. Thus, measuring up to its definition, virtual reality intervenes deeply in our lives. This study hereby investigates the practical use of virtual reality that could be experienced everyday.

심인성 색전 요인이 없는 뇌경색 환자에서 경식도 심초음파와 Acoustic Quantification을 이용한 대동맥 탄성의 평가

이종민,윤호중,임효영,박지원,김휘열,전희경,문건웅,정욱성,채장성,김재형,최규보,홍순조 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.8

상악소구치 과잉치와 하악 절치 과잉치에 대한 치험례

오민형,이광희,김대업,라지영 대한소아치과학회 2006 大韓小兒齒科學會誌 Vol.33 No.3

Gate 전압 변화에 의한 조셉슨 접합 배열에서의 초전도체-부도체 상전이 연구

이지형,차민철 漢陽大學校 工學技術硏究所 1998 工學技術論文集 Vol.7 No.1

2차원 박막의 초전도체-부도체 상전이에 대하여 boson Hubbard model을 통하여 알아본다. 특히 gate 전압을 통하여 boson의 밀도 변화로 유도되는 상전이에 대하여 살펴보고자 한다. 이를 위하여 (2+1)차원을 갖는 대등한 고전적인 모델을 택하여 Monte Carlo 방법을 통해 simulation한다. 이때 계의 크기를 변화시켜가며 물리량들을 측정하여 이로부터 무한 크기의 계에서의 상전이의 임계점을 찾아본다. 그 결과 초전도체 stiffness와 boson의 밀도에 대한 축척 곡선을 얻었으며 동역학적인 임계지수가 z= 2 임을 얻었다. We have studied superconductor-insulator phase transition of thin films through a boson Hubbard model. Especially we are interested in the transition caused by the change of boson density which may be controlled by gate voltage. For this purpose, Monte Carlo simulations of an equivalent (2+1)-dimensional classical model have been performed. Various physical quantities are measured while the size of systems are changed systematically in order to find the transition point in infinite system. As a result, we obtain fine scaling curves for superconductor stiffness and density of bosons. We also find that the dynamical critical exponent z=2.

이맞는 밀도에서 강한 Disorder가 있는 경우의 초전도체-부도체 상전이

이지형,차민철 한양대학교 이학기술연구소 1999 이학기술연구지 Vol.1 No.-

2차원 박막에서 T=0일 때 초전도체-부도체 상전이에 대하여 boson Hubbard model을 통하여 알아본다. 특히 integer filling에서(즉, μ=0인 경우) 강한 disorder가 주어져 있을 때, 임계지수의 값을 구한다. 이를 위하여 (2+1) 차원을 갖는 대등한 고전적인 모델을 택하여 Monte Carlo 방법을 통해 simulation 한다. 이때 동역학적인 임계지수를 가정하고, 계의 크기를 변화시켜가며 superconducting stiffness를 측정하여 임계지수를 찾아본다. 그 결과 μ≠0 이고 강한 disorder인 경우와 마찬가지고 z?2. v?0.9임을 얻었다. We have studied the zero-temperature superconductor-insulator phase transitions through a boson Hubbard model. Especially we are interested in the value of critical exponents when the bosons have integer fillings ( μ=0). For this purpose, Monte Carlo simulations of an equivalent (2+1) dimensional classical model have been performed. Assuming a dynamical critical exponent in advance, we measure the superconducting stiffness in various sizes of the systems in order to find the transition point. As a result, we obtain from finite-size scalings that z?2 and v?0.9, the same values as those for μ≠0 in the presence of strong disorder.

家畜排泄物 콤포스트化 有效利用에 關한 硏究

洪志亨,全炯柱,朴又龍,崔秉旻,金在煐 順天大學校 1983 論文集 Vol.2 No.1

家畜排泄物의 堆肥化 작업체계의 工學的인 局面은 畜産經營의 畜産物 生産性, 에너지保存, 公害防力및 勞動力 節約에 密接한 關係를 갖고 있는 바이다. 그러나 韓國에 있어서 現在 畜産業의 飼養規模와 飼育頭數는 每年 增加하는 傾向을 나타내고 있으나, 傳統的인 家畜排泄物의 堆肥化處理 加工作業上의 問題點에 關한 硏究資料는 거의 없는 實情으로 堆肥化 작업체계 改善確立이 切實히 要求되고 있다. 따라서, 本硏究의 目的은 家畜排泄物 堆肥化 작업체계상의 工學的인 諸問題點을 調査分析하고 이에 對한 現況을 評價하여 改善方案을 模索하기 위한 基本資料를 提供하기 위해 遂行되었으며 調査分析 結果를 要約하면 다음과 같다. 1. 畜産農家의 家畜排泄物 處分方法으로는 大部分이 自然乾燥, 堆積, 嫌氣的 固形堆肥化에 依하여 遂行되고 있어 家畜糞尿에 의한 公害가 深刻해 지고 있었다. 2. 野積堆肥化 가공작업체계에 있어서는 트랙터에 附着된 前後作動式 로우더가 바람직하다. 3. 好氣性 野積堆肥化 加工作業에 따른 良質의 腐熟堆肥를 얻기 위해서는 外部氣溫에 따라 適正通氣量을 維持하기 위해 3∼7日 間隔으로 混合攪拌해야 되며 氣象條件에 의한 影響을 防止하기 위하여 堆肥作業物質의 表面을 비닐 等으로 덮거나, 더운날에는 加水를 해야할 것이 要望된다. 4. 堆肥化 加工作業體系의 基本段階別로 이루어지는 畜産農家는 거의 찾아 볼 수가 없었다. 5. 堆肥化加工 過程은 畜産農家周圍의 콤포스트센타에서 全體農家가 地力增進과 公害防止를 위해 參與할 수 있는 總合的이고 協同的인 加工作業體系로서 一貫性있게 遂行되어야 할 것으로 思料된다. There was almost no livestock farms to follow the basic stage in composting operation system. Composting disposal process should be performed successively an integrate cooperative operation system at compost center which is participated in all farmers near the livestock farms for the purpose of land improvement and pollution abatement. Well matured compost which is resulted from aerobic windrow solid composting should be turned at 3 to 7 days to insure abequate aeration according to the ambient temperature. The compost material should be covered with vinyl sheets etc. to avoid the effect of climatic condition.