Exploring potential biomarker responses to lithium in Daphnia magna from the perspectives of function and signaling networks

Hyo Jeong Kim1,2,Jun Hyuek Yang,Hyun Soo Kim,Yeo Jin Kim,Wonhee Jang,Young Rok Seo,W. Jang,Y. R. Seo 대한독성 유전단백체 학회 2017 Molecular & cellular toxicology Vol.13 No.1

I ntensive usage of electronic appliances containing lithium batteries causes an accumulation of e-trash. Environmental exposure to lithium batteries contaminates ecosystems. In air and water, the batteries form lithium hydroxide (LiOH) on their surfaces. LiOH enters the aquatic environment and contaminates the aquatic ecosystem by being absorbed into biological organisms. In this study, in order to identify meaningful potential biomarkers that appear in response to lithium, we measured significantly up- and down-regulated genes after LiOH exposure by conducting a microarray. In addition, we explored the functions of differentially expressed daphnia genes, and we conducted a comparative analysis in other species, Daphnia spp. to humans, then analyzed the signaling pathways using the human gene set derived from daphnia sequences that are differentially expressed in response to LiOH using the NCBI-BLAST tool and Pathway studio. As a result, we identified signaling pathways and suggested several potential biomarkers that are up- or down-regulated in response to lithium. This study may contribute to the development of a biomonitoring system which can detect the ecotoxicity of lithium. Furthermore, lithium toxicity in humans can be predicted, so the study may also provide potential biomarkers of lithium exposure in humans.

흰쥐 삼차신경 척수감각핵 미측소핵 세포의 흥분성 시냅스 전도에 대한 nociceptin의 효과

김승록,최정희,송원정,천상우 원광대학교 치의학연구소 2003 圓光齒醫學 Vol.12 No.1

The caudal subnucleus of the spinal trigeminal nucleus (medullary dorsal horn; MDH) is implicated in the processing of nociceptive information in the orofacial region. In the present study, whole cell patch clamp recordings were carried out on MDH neurons in brainstem slice of neonatal rats to investigate the characteristics of spontaneous excitatory postsynaptic currents (sEPSCs) and the roles of nociceptin/orphanin FQ on excitatory synaptic transmission. In the presence of strychnine (1 μM) and bicuculline (20 μM), spontaneous inward currents at -70 mV were observed. The baseline frequency, amplitude and decay time constant of sEPSCs were 2.0 ± 0.5 Hz, 23.0 ± 1.8 pA, 3.8 ± 0.2 ms, respectively. sEPSCs were mediated by non-NMDA and NMDA receptors. The specific AMPA receptor antagonist GYKI-52466 (50 μM) completely blocked the non- NMDA mediated sEPSCs, indicating that they are mediated by an AMPA-preferring receptor. Perfusion of the opioid orphan receptor (ORL_1) adonist nociceptin (3 μM) reduced the frequency of sEPSCs and miniature EPSCs (mEPSCs). The effects of nociceptin were blocked by the selective ORL_1 receptor antagonist [Nphe'] nociceptin(1-13)NH_2 (3 μM) and were not affected by the non-specific opioid receptor antagonist naloxone (10 μM). To investigate the specificity of this synaptic inhibition, we selectively activated the nociceptive C fibers with capsaicin, which induced a strong increase in the frequency of sEPSCs. In the presence of nociceptin, the response to capsaicin was diminished. In conclusion, these results suggest that nociceptin inhibits excitatory synaptic transmission in the MDH by acting on presynaptic ORL_1 receptors.

습동면유 겸용 수용성 금속가공유제의 제조 및 특성 연구

윤을용,노정석,김해원,김선덕,진경록,김재권 한국공업화학회 2004 응용화학 Vol.8 No.2

At processing of a metal process, much problems are happening by mixing between slide-way fluid and water-solubility metal-working fluid(MWF). In this study, measure physical & chemical properties and biodegradable properties of base stock and additives that is used to lubricant, and developed water-soluble metal working fluid by slide-way fluid combined use. Biodegradability of developed combined use fluid for 28 days is displayed lower than 10 ppm, and more superior performance than similar fluids in lubrication special quality test.

LC-ESI-MS/MS를 이용한 생체시료 중 브롬화피나베리움의 고감도 분석 및 이를 이용한 생체이용률 평가 : Applicability to Oral Bioavailability Determination

박석,이예리,김호현,이희주,김윤균,염정록,한상범 한국약제학회 2004 Journal of Pharmaceutical Investigation Vol.34 No.6

A sensitive method for quantification of pinaverium bromide in human plasma was established using liquid chromatography-electrospray ionization tandem mass spectrometrv(LC-ESI-MS/MS). Glimepiride was used as internal standard. Pinaverium bromide and internal standard in plasma sample were extracted using tert-but}lmethvlether(TBME). A centrifuged upper laver was then evaporated and reconstituted with mobile phase of acetonitrile-5 m1VI ammonium formate (8020. pH 3.0). The reconstituted samples were injected into a C_(18) reversed-phase column. Using MS/MS with multiple reaction monitoring (MRM) mode. pinaverium and glimepirde were detected without severe interference from human plasma matrix. Pinaverium produced a protonated precursor ion ([M+H]^(+)) at m/z 510.3 and a corresponding product ion at m/z 228.9. Internal standard produced a protonated precursor ion ([M+H] ^(+)) at m/z 491.5 and a corresponding product ion at m/z 352.0. Detection of pinaverium bromide in human plasma was accurate and precise. with limit of quantitation at 0.5 ng/ml. The method has been successfully applied to bioavailability study of pinaverium bromide tablet in Korean healthy male volunteers. Pharmacokinetic parameters such as AUCr. C_(max) T_(max). K_(el) and t_(l/2) were calculated.

항파상풍 톡소이드 주사후 항파상풍 항체 역가

이한식,하영록,구홍두,장석준,심호식,김승환,이정운 대한응급의학회 1996 대한응급의학회지 Vol.7 No.2

Objective : Tetanus decreased in prevalence since the systemic vaccination began in 1940s. Despite of the improvement in treatment like critical care with ventilatior or antibiotics, the mortality rate resides around 45%. Currently the tetanus prevention protocol recommends immunization in infancy and boosters every tenth years. Thereafter the immunization with toxoid only or toxoid plus immunoglobulin injections is recommended according to the type of injury. In most of ED in Korea, only tetanus immunoglobulin is given without any basis. Previously we proved the effect that passive immunization with the immunoglubulin 250 IU last long for only a month. At this time we measured the effect of the active immunization with toxoid and its effect was compared to the effect of the tetanus immunoglobulin to find out the logical tetanus prevention after an injury. Method: 20 healthy adult volunteers were injected with tetanus toxoid and their anti-tetanus antibody titers measured before the injection, 1 week and 4 weeks after the inection. No volunteers have taken any medication for chronic illness(e.g. hepatits, tuberculosis) or had an tetanus immunization or booster in the last 6 months. Antibody titers were measured by IMMUNOZYM??-TETANUS and t-test was performed on the results, Results: 1. Total 20 volunteers(12 males and 8 females) were participated. 2. Subjects` ages were 21 to 44 years old and there were no relevance to the antibody titer. 3. The antibody titers of before the anti-tetanus toxoid injection and 1 week, 4 weeks after the injection revealed significant difference and the antibody titers of the first and the forth week after the injection also showed a significant difference. Conclusion: Currently in Korea, the tetenus immunoglobulin 250 IU given alone as tetanus prevention was injected, but it did not elevate the anti-tetanus antibody titer for 4 weeks where as tetanus toxoid injected intramuscularly did significantly. Therefore, tetanus toxoid should be given on the dirty injuries with additional tetanus immunoglobulin injection to accomplish the correct method of tetanus, tetanus toxoid prevention.

건강한 한국인 성인 남성에서 레보설피리드 제제의 생체이용률

이정민,최성업,김희규,윤미경,김세희,염정록,최영욱 한국약제학회 2003 Journal of Pharmaceutical Investigation Vol.33 No.3

Pharmacokinetics and oral bioavailability of levosulpiride was determined in Korean healthy male volunteers. Thirty subjects received a single oral dose (25 ㎎) of a tablet in randomized 2×2 cross-over design. The plasma concentratons of levosulpiride were measured by HPLC and compared with those reported in the literature. Pharacokinetic parameters for Isomeric^(??) tablet (levosulpiride 25 ㎎) were revealed as follows: AUC _(inf) 737.1±176.9 ng·hr/ml, C_(max) 56.4±20.1 ng/ml, T_(max) 4.2±1.6hr, K_(a) 1.00±1.09 hr^(-1), K_(el) 0.08±0.02 hr^(-1), and t_(1/2) 8.8±1.9 hr. The rate constant of the absorption phase was obtained based on the first-order kinetics. In the aspect of bioavailability, Isomeric^(??) tablet was bioequivalent to the other product (Levopride^(?)tablet) available in the Korean market. Intersubject variations and race differences were shown in comparison with the published date in the literature, even though there ws a linear relationship between dose and extent of bioavailability.

The Relative and Incremental Information Content of EVA as a Corporate Performance Measure in Korean Capital Market

Kim, Jeong Kyo,Lee, Jeong Rok 釜山 大學校 經營 經濟 硏究所 2000 經營 經濟 硏究 Vol.19 No.1

This paper investigates the relative and incremental information contents of EVA compared to the other performance measures : net income, operating income after taxes, and cash flows from operating activities which are widely used in investment and business communities. The relative information content results show no evidence that EVA has more explanatory power concerning changes in stock returns than earnings and operating cash flow. Contrary to the prior conjecture, in most cases the evidence suggests that net income has higher relative information content than EVA. I can not detect stronger value-relevance for EVA in the sensitivity analysis. On the other hand, the incremental information content results suggest that EVA provides additional explanatory power concerning changes in stock returns beyond earnings and operating cash flow. When the results of two kinds of information content test are taken together, it is concludend that, while EVA provides some incremental information content beyond earnings and operating cash flow, it does not provide greater relative information content than earnings.

The novel prognostic marker, EHMT2, is involved in cell proliferation via HSPD1 regulation in breast cancer

Kim, Seon-Kyu,Kim, Kwangho,Ryu, Jea-Woon,Ryu, Tae-Young,Lim, Jung Hwa,Oh, Jung-Hwa,Min, Jeong-Ki,Jung, Cho-Rok,Hamamoto, Ryuji,Son, Mi-Young,Kim, Dae-Soo,Cho, Hyun-Soo D.A. Spandidos 2018 International journal of oncology Vol.54 No.1

<P>Molecular classifications of breast cancer (BRC), such as human epidermal growth factor receptor 2 (HER2), luminal A and luminal B, have been developed to reduce unnecessary treatment by dividing patients with BRC into low- and high-risk progression groups. However, these methods do not cover all of the pathological characteristics of BRC, and investigations into novel prognostic/therapeutic markers are thus continually required. In this study, we identified the overexpression of the histone methyltransferase, euchromatic histone-lysine N-methyltransferase 2 (EHMT2) in BRC samples (n=1,222) and normal samples (n=113) derived from the TCGA portal by performing a BRC tissue microarray. EHMT2 overexpression was clearly associated with a poor prognosis in multiple cohorts of patients with BRC (total, n=1,644). Furthermore, the knockdown of EHMT2 expression affected cell apoptosis via the downregulation and re-localization of heat shock protein family D (Hsp60) member 1 (HSPD1). In addition, a statistically significant positive correlation between EHMT2 and HSPD1 expression was revealed in the clinical cohorts. On the whole, the findings of this study may assist the development of novel therapeutic strategies and provide a prognostic marker (EHMT2) for patients with BRC.</P>

Photon activation analysis of NIST SRM sediment sample using the electron linear accelerator at Pohang Accelerator Laboratory

Kim, HyoJin,Noh, Sung Jin,Kim, Hyun,Jeong, Dong Hyeok,Yang, Kwangmo,Kim, Guinyun,Kang, Yeong-Rok Springer-Verlag 2018 JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY Vol.316 No.3

Multi-Valued Logic Device Technology; Overview, Status, and Its Future for Peta-Scale Information Density

Kim, Kyung Rok,Jeong, Jae Won,Choi, Young-Eun,Kim, Woo-Seok,Chang, Jiwon The Institute of Semiconductor Engineers 2020 Journal of semiconductor engineering Vol.1 No.1

Complementary metal-oxide-semiconductor (CMOS) technology is now facing a power scaling limit to increase integration density. Since 1970s, multi-valued logic (MVL) has been considered as promising alternative to resolve power scaling challenge for increasing information density up to peta-scale level by reducing the system complexity. Over the past several decades, however, a power-scalable and mass-producible MVL technology has been absent so that MVL circuit and system implementation have been delayed. Recently, compact MVL device researches incorporating multiple-switching characteristics in a single device such as 2D heterojunction-based negative-differential resistance (NDR)/transconductance (NDT) devices and quantum-dot/superlattices-based constant intermediate current have been actively performed. Meanwhile, wafer-scale, energy-efficient and variation-tolerant ternary-CMOS (T-CMOS) technology has been demonstrated through commercial foundry. In this review paper, an overview for MVL development history including recent studies will be presented. Then, the status and its future research direction of MVL technology will be discussed focusing on the T-CMOS technology for peta-scale information processing in semiconductor chip.