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고정환 ; 윤옥현 ; 박홍기 ; 전기환 ; 김상민 ; 김학민 ; 박희룡 김천대학교 1999 김천대학교 논문집 Vol.20 No.-
The social education that is provided for the people will be an important part of the national growth in the 21 century. This study was taken to present the desirable direction that Kimcheon College Continuing Education has to make progress as a central organization with perceiving the importance of continuing education for the Kimcheon citizen. To achieve the objective of the study, examined a sample of 592 citizen and students from Kimcheon City and Kimcheon College. The survey revealed the following results : 1. Offer the effective operation of education policies and curriculums. 2. Perform the role of foreign language education center. 3. Introduce the saving credit system. 4. Operate the special lectures for the students of the high school graduating class after the national scholastic achievement test for university and college entrance. 5. Suppert the special skills education for high school students after-school hours. 6. Administer the new employment program. These results showed the desirable direction that the Kimcheon College Continuing Education has to take some actions for the Kimcheon citizen.
Developments of cancer biomakers : a glyco-oriented approach
Jeong-Heon Ko 한국당과학회 2009 한국당과학회 학술대회 Vol.2009 No.1
Cancer is often difficult to early diagnose, but early diagnosis is a crucial factor for good outcome in the cancer. N-acetylglucosaminyltransferase V catalyzes an addition of b1,6-N-acetylglucosamine (GlcNAc) to the core N-glycan, many lines of evidence have demonstrated the role of N-acetylglucosaminyltransferase V (GnT-V) in the pathogenecity of colon cancer cell. One of the related example is an aberrant TIMP-1-mediated cancer progression in which deterioration of the quality of TIMP-1 is induced by glycosyl alterations through GnT-V catalysis, leading to mitigated MMP inhibition responsible for an strengthened invasive/metastatic potential of cancer cells. Accordingly the identification of target proteins for GnT-V has been an important subject for cancer biomarker discovery. We developed a protocol in which L-PHA, a lectin recognizing b1,6-GlcNAc, is conjugated to avidin-agarose complex to capture b1,6-GlcNAc-carrying serological glycoproteins and the captured glycoproteins were identified using an LTQ-FTICR mass spectrometer. Candidate proteins showing differential amounts between normal and cancer sera were confirmed by western blot analysis and will be subject to validation for valid biomarker for colon cancer.
Development of cancer glyco-biomarkers and its applications
Ko, Jeong Heon 한국당과학회 2016 한국당과학회 학술대회 Vol.2016 No.07
Cancer is often difficult to achieve early diagnosis, which is, however, a decisive requisite for favorable outcome in cancer treatments. Cancer biomarkers have been sought for several purposes preferably in blood and pinpointing cancer cells-derived aberrant glycoproteins would be a well-grounded approach to cancer biomarker discovery. One of the glycosyltransferases responsible for aberrant glycosylation in cancer is N-acetylglucosaminyltransferase V (GnT-V), which catalyzes an addition of b1,6-N-acetylglucosamine (GlcNAc) to the core N-glycan, and many lines of evidence have demonstrated the role of N-acetylglucosaminyltransferase V (GnT-V) in cancer development. Tissue inhibitor of metalloproteinase-1 (TIMP-1) and protein tyrosine phosphatase kappa (PTPk) were suggested to be involved in cancer malignancy upon aberrantly glycosylation by GnT-V. In addition to GnT-V, several glycosyltransferase co-works to render the altered glycan structures in cancer cells including sialyl Lewis antigen and core-fucosylation, which prompted us to mine serological biomarker candidates in cancer sera. Immunodepleted on an immune-LC column, serological proteins were enriched by carbohydrate beads conjugated with lectins like L-PHA, DSA, E-selectin, AAL, Con-A. The fraction refractive to lectin enrichments was resolved on an SDS-PAGE gel, and fractionated by molecular mass. Both the captured glycoproteins and gel-separated proteins were tryptic-digested for sequence determination in an LTQ-FTICR mass spectrometer. Candidate proteins showing high sensitivity and specificity during the discovery phase were selected and the panel of biomarker candidates is currently under in-depth analyses for validation.
Jeong, Hyun-Ja,Lee, Ju-Young,Kim, Joon-Bae,Go, Hoyeon,Ko, Seong-Gyu,Seo, Young-Wan,Jeong, Sejin,Park, Jinhan,Na, Ho-Jeong,Um, Jae-Young,Kim, Hyung-Min,Hong, Seung-Heon Gordon and Breach 2008 INTERNATIONAL JOURNAL OF NEUROSCIENCE - Vol.118 No.10
<P>KI0477959 (Herbkines) has been used for the purpose of development of physical strength in wasting diseases, like cancer. In the present study, apoptosis-inducing activities of butanol fraction of KI0477959 were studied in human leukemia cell line, HL-60 cells. KI0477959 increased cytotoxicity but had less effect on human peripheral blood mononuclear cells. KI0477959-induced apoptosis was accompanied by activation of caspase-3 and specific proteolytic cleavage of poly-ADP-ribose polymerase. Increased apoptosis was reduced by treatment with p38 and extracellular signal-regulated protein kinase (ERK) inhibitors. These results suggest that KI0477959 induces apoptosis through activation of caspase-3, p38, and ERK in HL-60 cells.</P>
Achievements and vision of the global network for cancer biomarker development
Jeong-Heon Ko 한국당과학회 2011 한국당과학회 학술대회 Vol.2011 No.1
Our center, Daejeon-KRIBB-FHCRC Research Cooperation Center, was established to develop ‘cancer biomarkers’ in collaboration with Fred Hutchinson Cancer Research Center (FHCRC) in Feb. 2005 with supports from the Daejeon Metropolitan City. Cancer is often difficult to achieve early diagnosis, which is, however, a decisive requisite for favorable outcome in cancer treatments. In order to develop the cancer biomarkers, we’ve tried to pinpoint cancer cells-originating aberrant glycoproteins which would be a well-grounded approach to cancer biomarker discovery ultimately using blood. One of the glycosyltransferases responsible for aberrant glycosylation in cancer is N-acetylglucosaminyltransferase V (GnT-V), which catalyzes an addition of b1,6-N-acetylglucosamine (GlcNAc) to the core N-glycan, and many lines of evidence have demonstrated the role of N-acetylglucosaminyltransferase V (GnT-V) in cancer development. Tissue inhibitor of metalloproteinase-1 (TIMP-1) and protein tyrosine phosphatase kappa (PTPk) were good models involved in cancer malignancy upon aberrantly glycosylation. Basically by adopting multi-lectins enrichment strategy, candidate proteins showing high sensitivity and specificity during the discovery phase were selected and the panel of biomarker candidates is currently under in-depth analyses for validation. Validation is a time-consuming step for biomarker developments requiring confirmation of the biomarker candidates through multiple pairs of clinical samples. That is why a sensitive, multiplexing validation method is necessary. To address this challenge, we are developing as a DNA-tagged antibody-based ‘New’ validation method, enabling a multiplexed validation of biomarkers. Furthermore, the collaboration with the FHCRC leads to an expansion of the ‘epigenetic’ studies to ‘glycogenome’, requiring incessant collaborations. Recently, Daejeon Metropolitan City became a ‘sister city’ for Sapporo City in Japan. It’s noteworthy that these relationships prompt to establish a ‘north pacific network of cancer biomarker development’ among the KRIBB, FHCRC and Sapporo Medical University.