Plant regeneration from callus of Iris odaesanensis Y. N. Lee native to Korea via organogenesis

Bae, Kee-Hwa,Yoo, Kyoung-Hwa,Lee, Mi-Hyun,Jeong, Jae-Hun,Choi, Yong-Eui,Yoon, Eui-Soo The Korean Society of Plant Biotechnology 2013 식물생명공학회지 Vol.40 No.3

Iris odaesanensis Y. N. Lee. is an important endangered and native plant belonging to the family Iridaceae in Korea. This study describes a method for rapid micropropagation of this species via from leaf, rhizome and root explants derived calli. Leaf, rhizome and root explants were cultured on Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxy acetic acid (2,4-D) for callus induction. Rhizome explants yielded calli at a frequency of 72% when cultured at 1.0 mg/l 2,4-D. Calli were maintained at 1.0 mg/l 2,4-D. These calli were transferred to MS medium supplemented with 0, 0.5, 1.0, and 2.0 mg/l 2,4-D in combination with 0, 0.5, 1.0, and 3.0 mg/l BA for adventitious shoot induction. The highest number of adventitious shoot (228.9 per petri-dish) were formed at 1.0 mg/l 2,4-D and 1.0 mg/l BA. WPM medium was the best to convert calli into plantlets, where up to 98.2% of calli were regenerated into plantlets. This in vitro propagation protocol should be useful for conservation of this endangered plant.

간호 학생의 임상실습 효율을 위한 기초 조사 연구

이애경,김정애,주미경,정안순,배경진,김정수,이정애,장은정 경복대학 2000 京福論叢 Vol.4 No.-

임상실습 교육은 간호이론을 실무에 적용하는 기회를 제공하는 과정으로서 임상실습이 간호교육의 중요한비중을 차지하고 있다는 것은 주지의 사실이다. 이러한 중요성에도 불구하고, 임상실습 교육에 즉각적인 해결이 어려운 많은 문제점이 있음도 이미 알고 있는 사실이다 이에 임상실습 교육의 기초 자료로 활용되고 있는 실습목록표에 대한 간호 학생들의 실습목표의 명확한 이해와 더불어 이론과 실습의 연계성을 강화시키고자 하였다. 성인간호, 모성간호, 이동간호 3개 영역 모두에서 공통적으로 기본임상간호 항목이나 기본간호술과 관련된 항목에서 직접수행의 빈도가 높았고 숙련된 기술적인 처치나 전문성을 필요로 하는 항목에서는 관찰과 수행경험이 부족한 것으로 나타났다. Clinical performance is not only the process of providing the opportunity for applying nursing theory into practice but also it is a well-known fact that clinical performance is an important part in nursing education. lnspite of this importance, it is also true that many problem with no immediate solution exist in clinical performance of nursing students. thus, through measuring and analyzing the degree of clinical experience of the nursing students, on the nursing checklist that is being used as the basic guide in clinical education, we tried to clearly understand the objectives of clinical performance and to emphasize the connection between theory and clinical performance. The results of the study showed that the frequency of directly performing tasks was high in all areas of adult health nursing, maternity nursing, and child nursing for the items of basics clinical nursing and items related with basic nursing techniques, and that the experiences of observation and execution were lacking in the items needing skilled treatment or speciality.

Dimethylnitrosamine 유발 급성 간 손상 흰쥐에서 ^(99m)Tc-Lactosylated Serum Albumin을 이용한 간 기능의 평가

정신영,이재태,서명랑,유정아,배진호,안병철,황재석,정재민,하정희,이규보 대한핵의학회 2003 핵의학 분자영상 Vol.37 No.6

목적: ^(99m)Tc-lactosylated serum albumin (^(99m)Tc-LSA)은 간세포에 특이적으로 결합하는 간수용체 영상용 방사성의약품으로 새로이 합성되었다. 간섬유화를 유발하는 dimethylnitrosamine (DMN)을 투여한 간 손상 휜쥐 모델에서 ^(99m)Tc-LSa의 역동학적인 간섭취를 조사하고 간효소치의 변화와 조직학적 소견을 비교하여, LSA의 간섭취가 간기능의 변화를 반영하는지를 연구하였다. 대상 및 방법: SD계 흰쥐에 DMN를 27 mg/kg으로 복강 내 주사하여 급성 간손상을 유도하고 대조군과 비교하였다. DMN을 주사한 흰쥐를 3일(DMN-3), 8일(DMN-8), 21일(DMN-21)에 ^(99m)Tc=LSA (1,665 mg/kg) 29 MBq를 정맥 주사하여, 30분 동안 동적 영상을 획득하고 간과 신장부위에 관심영역을 설정하여 간과 심장부위의 시간방사능 곡선을 얻었다. 간기능 평가를 위해 시간방사능 곡선을 이용하여 간섭취지수와 혈중제거지수를 구하였고 곡선 최적화를 시행하였다. DMN 투여군과 대조군의 간효소치의 변화와 간조직의 광학현미경 소견을 비교하였다. 결과: 대조군에서는 ^(99m)Tc-LSA가 빠르게 간에 섭취되고 혈중에서 제거되었으나 DMN을 처리한 군에서는 간섭취가 낮았다. 간섭취지수의 비교에서 대조군에 비해 DMN 처리군에서 유의하게 간섭취지수가 낮았다(DMN-3: 0.842, DMN-8: 0.898, DMN-21: 0.91, 대조군: 0.96, p<0.05). 혈중제거지수의 비교에서도 대조군에 비해 DMN 처리군에서 혈중제거지수가 유의하게 높았다(DMN-3: 0.731, DMN-8: 0.654, DMN-21: 0.604, 대조군: 0.473, p<0.05). 비선형 회귀분석에서 R_(2) 값은 0.9이상으로 좋은 일치를 보였고, 대조군에ㅓ K값이 DMN처리군에 비해 크고(DMN-3: 0.28, DMN-8: 0.41, DMN-21: 0.46, 대조군: 0.97, p<0.05), T_(1/2)값은 작았다(DMN-3: 2.5, DMN-8: 1.7, DMN-21: 1.5, 대조군: 0.7, p<0.05). 간효소치의 변화는 DMN-3군에서는 대조군에 비해 상승하였으나 DMN-8, DMN-21군에서는 간효소치의 상승이 관찰되지 않았다. 간조직 소견의 경우 DMN-3군에서 중심정맥 주위에 괴사가 관찰되었으나 DMN-8군, DMN-21군에서는 미약한 정도의 염증세포 침윤만이 관찰되었다. 결론: ^(99m)Tc-LSA 간신티그래피의 간섭취 정도는 간손상과 반비례하였으며 간섭취의 변화는 조직학적 손상이 심한 정도와 간손상후 회복되는 과정을 반영하여 주었다. ^(99m)Tc-LSA 간신티그래피가 간손상을 평가하고 간손상후 회복되는 과정을 추적하는 간수용체 영상용 방사성 의약품으로 사용될 수 있을 것으로 생각된다. Objects: ^(99m)Tc-lactosylated human serum albumin(LSA) is a newly synthesized radiopharmaceutical that binds to asialoglycoprotein receptors, which are specifically presented on the hepatocyte membrane. Hepatic uptake and blood clearance of LSA were evaluated in rat with acute hepatic injury induced by dimethylnitrosamine(DMN) and results were compared with corresponding findings of liver enzyme profile and these of histologic changes. Materials and Methods: DMN (27 mg/kg) was injected intraperitoneally in Sprague-Dawley rat to induce acute hepatic injury. At 3(DMN-3), 8(DMN-8), and 21(DMN-21) days after injection of DMN, LSA injected intravenously, and dynamic images of the liver and heart were recorded for 30 minutes. Time-activity curves of the heart and liver were generated from regions of interest drawn over liver and heart area. Degree of hepatic uptake and blood clearance of LSA were evaluated with visual interpretation and semiquantitative analysis using parameters (receptor index : LHL3 and index of blood clearance : HH3), analysis of time-activity curve was also performed with curve fitting using Prism program. Results: Visual assessment of LSA images revealed decreased hepatic uptake in DMN treated rat, compared to control group. In semiquantitative analysis, LHL3 was significantly lower in DMN treated rat group than control rat group (DMN-3:0.842, DMN-8: 0.898, DMN-21: 0.91, Control: 0.96, p<0.05), whereas HH3 was significantly higher than control rat group (DMN-3: 0.731, DMN-8: 0.654, DMN-21: 0.604, Control: 0.473, p<0.05). AST and ALT were significantly higher in DMN-3 group than those of control group. Centrilobular necrosis and infiltration of inflammatory cells were most prominent in DMN-3 group, and were decreased over time. Conclusion: The degree of hepatic uptake of LSA was inversely correlated with liver transaminase and degree of histologic liver injury in rat with acute hepatic injury.

보존적 치료로 회복된 망경색에 의한 급성복증 1예

김정념,이숭환,김정식,김진호,배윤오,박성규,윤상정,한현영,이헌영 충남대학교 의과대학 의학연구소 2003 충남의대잡지 Vol.30 No.1

Omental infarction, the end result of impaired perfusion to the greater omentum, is a rare benign self-limiting clinical entity. The main clinical symptom is non-specific localized abdominal pain with a moderately raised white blood cell and erythrocyte sedimentation rate. These findings often mimic an abdominal surgical emergency. This condition is often misdiagnosed as acute appendicitis or cholecystitis. The characteristic feature of CT scan and ultrasonography provide non-invasive diagnosis in most patients with omental infarction. We report a case of patient whose CT scan showed the characteristic finding of omental infarction. The patient was improved spontaneously only with conservative care.

Bortezomib과 Dexamthasone으로 치료한 골수외 형질세포종 4예

백종현,이은영,장리라,손창배,신은경,서정아,이지숙,이호섭,이상민,신성훈,김양수 고신대학교의과대학 2007 고신대학교 의과대학 학술지 Vol.22 No.2

Despite the use of aggressive local and systemic treatment including autologous stem cell transplantation in multiple myeloma, extramedullary recurrences are common and the prognosis of these patients is poor. Many novel drugs such as thalidomide, lenalidomide and bortezomib improve the response of treatment of multiple myeloma, but some reports failed to describe thalidomide has effect in extramedullary plasmacytoma. Recent data report on the successful treatment plasmacytomas with bortezomib in patients with advanced multiple myeloma. We treated 4 relapsed or refractory extramedullary plasmacytomas with bortezomib at our institution. We recognized all these extramedullary plasmacytomas decreased and showed more than partial response. This report lends support to the efficacy of bortezomib in the treatment of plasmacytoma and describes the safe use of bortezomib. Responses may, however, be of short duration. Therefore, despite our limited experience, we propose that bortezomib may be considered a therapeutic option for such patients who have risk of radiation therapy

일차배양된 설치류 호흡기 상피세포로부터의 점액소 분비에 대한 수종 약물의 영향

이충재,석정호,이재흔,허강민,박지선,배소현,노삼길,박상철 충남대학교 의학연구소 2003 충남의대잡지 Vol.30 No.2

1. PKC activator인 PMA는 일차배양 HTSE세포로부터의 뮤신분비를 0.1μM 농도에서 30%, 1μM 농도에서 80% 가량 증가시켰다. 2. 식물 유래 성분으로, flavonoid의 일종인 TFR은 일치배양 HTSE 세포로부터의 뮤신분비를 10μM 농도에서 50%, 100μM 농도에서 80% 가량 증가시켰다. 3. 양이온성 폴리펩티드인 PLL 및 PLA는 일차 배양 HTSE 세포로부터의 뮤신분비를 0.01 - 10μM 농도에서 용량의존적으로 감소시켰다. 4. 결론적으로, 본 연구에서 얻어진 결과들은 새로운 거담제 및 점액용해제나 단백분해 효소제가 아닌 호흡기 류신의 생성/분비를 조절해 줄 수 있는 신개념의 약물을 개발함에 있어 극히 일부분이나마 단서를 제공하고 있다고 하루 수 있을 것이다. In the present study, we tried to investigate whether phorbol myristate acetate(PMA), trihydroxymethoxy-flavanone rutinoside(TFR) and cationic polypeptides significantly affect mucin release(secretion) from cultured hamster tracheal surface epithelial cells. Confluent primary hamster tracheal surface epithelial (HTSE) cells were metabolically radiolabeled with 3H-glucosamine for 24 hr and chased for 30 min in the presence of each agent to assess the effect on 3H-mucin release. The results were as follows : (1) Both PMA and TFR significantly increased mucin release from cultured HTSE cells ; (2) Cationic polypeptides including po1y-L-lysine(PLL, mw 7,500) and poly-L-arginine(PLA, mw 10,800) significantly inhibited mucin release from cultured HTSE cells, in a dose-dependent manner. This finding suggests us that PMA and TFR be further studied for the possible use as mild expectorants and cationic polypeptides might function as a regulator for hyper-secretion of mucus, both by direct acting on airway mucin-secreting cells, during the treatment of chronic airway diseases.

PVC의 유리전이 온도에 대한 가소제의 영향

이윤배,최정현,원상영 순천향대학교 부설 산업기술연구소 1997 순천향 산업기술연구소논문집 Vol.3 No.1

The plasticizer effect of poly(propylene carbonate) (PPC) on glass transition temperatures of po1y(vinyl chloride) (PVC) has been investigated and compared with dioctyl phthalate (DOP), a common plasticizer of PVC. The decrease in glass transition temperatures of the PPC blended PVC was not significant when compared with that of DOP. For the theoretical calculation of solubility parameters group molar attraction constants have been employed. The PPC performance was within an acceptable range for some type of application.

열대지방사람들의 땀샘의 밀도와 기능

이정범,배준상,민영기,양훈모 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1

Tropical inhabitants are able to tolerate heat through permanent residence in the tropics. To clarify the peripheral mechanisms of the suppressed thermal sweating in tropical natives, sweating responses to acetylcholine (ACh), a primary transmitter of the sudomotor innervation, were compared between healthy Korean and African males. ACh was iontophoretically administered on the forearm. Directly activated and axon reflex-mediated sweat responses were evaluated by quantitative sudomotor axon reflex test (QSART). The sweating of axon reflex (nicotinic receptor) onset-time was 0.42 min shorter (P<0.01) and the sweat volumes (muscarinic receptors) were 75% (P<0.01) and the ACh sweat volumes of axon reflex (nicotinic receptor) 79% higher (P<0.01) in the Koreans than the Africans. Iodine-impregnated paper method revealed that sweat gland density was 45.1% higher (P<0.01) and sweat gland output per single gland was 18.4% larger (P<0.001) in the Koreans compared to the Africans. These results indicate that suppressed thermal sweating in Africans is, at least in part, attributed to the suppressed glandular sensitivity to ACh through both recruitment of sweat glands and sweat output per each gland.

플라톤의 양생법연구

이동건,배정하 東亞大學校附設스포츠科學硏究所 2002 스포츠科學硏究論文集 Vol.20 No.-

The purpose of this study was to investigate the Plato's Yangseng Method. The Yangseng Method means the therapeutic method without medicine. The following paragraph is thought to best summarize Plato's thought of Yangseng. 1. His Yangseng Method has the exercise of the goodness of disposition. 2. The other method is the nutrition of the simplicity. 3. Tnother method is the sex of the well disposition. Therefore, the three requisites for the health of Plato is the goodness of disposition in the Exercise, the Nutrition and the sex.

2000년 이후 주요 학술지에 발표된 떡 연구 고찰

배정숙,이경은 서울여자대학교 자연과학연구소 2013 자연과학연구논문집 Vol.25 No.-

The purposes of the study were to review research on dduk (Korean traditional rice cake) published in two academic journals (Journal of the Korean Society of Food and Cookery Science and Journal of the Korean Society of Food Culture) for year 2000-2012 and to provide research suggestions for future research. A total of 171 articles were reviewed and analyzed by research purpose, research method, type of dduk, and ingredients. All of the research was conducted on dduks which were prepared by steaming or punching. In the same context, sulgidduk and jeungpyeon were the most frequently studied. The research purposes were quality characteristics evaluation and standardized recipe development, functionality of ingredients, customer’s perception on dduk, microbiological assessment, packaging and storage, and review of old literature. Most research used regular rice as a main ingredient and various secondary ingredients were used. Based on the result, several suggestions were provided for future research. The result will guide researchers to plan research projects on dduk and an infrastructure is needed that bridges between the needs of the researchers and practitioners in the dduk industry.