PLASMA EFFECTS ON HUMAN LIVER CANCER CELLS

Daeyeon Kim,Bomi Gweon,Danbee Kim,Sukhyun Song,Mina Kim,Se Youn Moon,Wonho Choe,Jennifer H. Shin 대한기계학회 2008 대한기계학회 춘추학술대회 Vol.2008 No.5

Low pressure plasma has been widely used in surface treatment of solid materials. In recent years, atmospheric plasma (cold plasma) is introduced in biomedical applications. In general, many types of cellular behaviors depend on the plasma density or intensity. While the plasma-treated cells are detached because of the plasma-induced damage of cell adhesion molecules at low plasma density, the cell membranes are destructed and the necrosis of cells occurs at high plasma density. In this research, we investigate the effects of plasma on cancerous cells. We treat the target human liver cancer cells (HepG2 and SK-Hep1) using a He plasma needle device, which has a pin type plasma jet. We aim to identify the plasma effects on cancerous cells as a function of the treatment time and applied power. This study may contribute to the development of a novel cancer therapy.

Communicating Healthy Food Choice

Sang-Yeon Kim,Jennifer Morey Hawkins,Hayeon Song,Tae-Seop Lim,Emily McCarthy. Cramer,Seokhoon Ahn,Jihyun Kim,Hyun-Joo Kim,Junghyun Kim,Hiroshi Ota 한국언론학회 2016 Asian Communication Research Vol.13 No.1

This research examines cultural influence on the preference of promotive (i.e., trying to eat more healthy foods) and preventive (i.e., trying to avoid unhealthy foods) dietary practices. Survey data were collected from students and older adults in Korea (N = 287) and the U.S. (N = 245). Only students participated in the survey in Japan (N = 145). Consistent with the prediction, Study 1 shows Korean mature adults are more willing to adopt preventive dietary practices, while American counterparts favor promotive eating habits. The pattern of the data was inconsistent with the prediction among student participants across the three nations. Individual-level data from Study 2 (N = 284) confirm that independent self-construal affects promotive dietary practice via increasing promotive regulatory self-focus. The indirect impact of interdependent self-construal on preventive dietary practice was less apparent. Practical implications for doctor-patient communication are discussed.

Toe Tissue Transfer for Reconstruction of Damaged Digits due to Electrical Burns

Kim, Hyung-Do,Hwang, So-Min,Lim, Kwang-Ryeol,Jung, Yong-Hui,Ahn, Sung-Min,Song, Jennifer K. Korean Society of Plastic and Reconstructive Surge 2012 Archives of Plastic Surgery Vol.39 No.2

Background : Electrical burns are one of the most devastating types of injuries, and can be characterized by the conduction of electric current through the deeper soft tissue such as vessels, nerves, muscles, and bones. For that reason, the extent of an electric burn is very frequently underestimated on initial impression. Methods : From July 1999 to June 2006, we performed 15 cases of toe tissue transfer for the reconstruction of finger defects caused by electrical burns. We performed preoperative range of motion exercise, early excision, and coverage of the digital defect with toe tissue transfer. Results : We obtained satisfactory results in both functional and aesthetic aspects in all 15 cases without specific complications. Static two-point discrimination results in the transferred toe cases ranged from 8 to 11 mm, with an average of 9.5 mm. The mean range of motion of the transferred toe was $20^{\circ}$ to $36^{\circ}$ in the distal interphalangeal joint, $16^{\circ}$ to $45^{\circ}$ in the proximal interphalangeal joint, and $15^{\circ}$ to $35^{\circ}$ in the metacarpophalangeal joint. All of the patients were relatively satisfied with the function and appearance of their new digits. Conclusions : The strategic management of electrical injury to the hands can be both challenging and complex. Because the optimal surgical method is free tissue transfer, maintenance of vascular integrity among various physiological changes works as a determining factor for the postoperative outcome following the reconstruction.

Trusting Social Media as a Source of Health Information: Online Surveys Comparing the United States, Korea, and Hong Kong

Song, Hayeon,Omori, Kikuko,Kim, Jihyun,Tenzek, Kelly E,Hawkins, Jennifer Morey,Lin, Wan-Ying,Kim, Yong-Chan,Jung, Joo-Young JMIR Publications Inc. 2016 Journal of medical Internet research Vol.18 No.3

<P><B>Background</B></P><P>The Internet has increasingly become a popular source of health information by connecting individuals with health content, experts, and support. More and more, individuals turn to social media and Internet sites to share health information and experiences. Although online health information seeking occurs worldwide, limited empirical studies exist examining cross-cultural differences in perceptions about user-generated, experience-based information compared to expertise-based information sources.</P><P><B>Objective</B></P><P>To investigate if cultural variations exist in patterns of online health information seeking, specifically in perceptions of online health information sources. It was hypothesized that Koreans and Hongkongers, compared to Americans, would be more likely to trust and use experience-based knowledge shared in social Internet sites, such as social media and online support groups. Conversely, Americans, compared to Koreans and Hongkongers, would value expertise-based knowledge prepared and approved by doctors or professional health providers more.</P><P><B>Methods</B></P><P>Survey questionnaires were developed in English first and then translated into Korean and Chinese. The back-translation method ensured the standardization of questions. Surveys were administered using a standardized recruitment strategy and data collection methods.</P><P><B>Results</B></P><P>A total of 826 participants living in metropolitan areas from the United States (n=301), Korea (n=179), and Hong Kong (n=337) participated in the study. We found significant cultural differences in information processing preferences for online health information. A planned contrast test revealed that Koreans and Hongkongers showed more trust in experience-based health information sources (blogs: <I>t</I><SUB>451.50</SUB>=11.21, <I>P</I><.001; online support group: <I>t</I><SUB>455.71</SUB>=9.30, <I>P</I><.001; social networking sites [SNS]: <I>t</I><SUB>466.75</SUB>=11.36, <I>P</I><.001) and also reported using blogs (<I>t</I><SUB>515.31</SUB>=6.67, <I>P</I><.001) and SNS (<I>t</I><SUB>529.22</SUB>=4.51, <I>P</I><.001) more frequently than Americans. Americans showed a stronger preference for using expertise-based information sources (eg, WebMD and CDC) compared to Koreans and Hongkongers (<I>t</I><SUB>360.02</SUB>=3.01, <I>P</I>=.003). Trust in expertise-based information sources was universal, demonstrating no cultural differences (Brown-Forsythe <I>F</I><SUB>2,654</SUB>=1.82, <I>P</I>=.16). Culture also contributed significantly to differences in searching information on behalf of family members (<I>t</I><SUB>480.38</SUB>=5.99, <I>P</I><.001) as well as to the goals of information searching.</P><P><B>Conclusions</B></P><P>This research found significant cultural differences in information processing preferences for online health information. Further discussion is included regarding effective communication strategies in providing quality health information.</P>

Collaborative effects of electric field and fluid shear stress on fibroblast migration.

Song, Sukhyun,Han, Hana,Ko, Ung Hyun,Kim, Jaemin,Shin, Jennifer H Royal Society of Chemistry 2013 Lab on a chip Vol.13 No.8

<P>Cells are inherently exposed to a number of different biophysical stimuli such as electric fields, shear stress, and tensile or compressive stress from the extracellular environment in vivo. Each of these biophysical cues can work simultaneously or independently to regulate cellular functions and tissue integrity in both physiological and pathological conditions. Thus, it is vital to understand the interaction of multiple stimuli on cells by decoupling and coupling the stimuli in simple combinations and by investigating cellular behaviors in response to these cues. Here, we report a novel microfluidic platform to apply the combinatorial stimulation of an electric field and fluid shear stress by controlling two directional cues independently. An integrated microfluidic platform was developed using soft lithography to monitor the cellular migration in real-time in response to an electric field and fluid shear stress in single, simultaneous, and sequential modes. When each of these stimulations is applied separately, normal human dermal fibroblasts migrate toward the anode and in the direction of fluid flow in a dose-dependent manner. Simultaneous stimulation with an electric field and shear stress, which mimics a wound in vivo, enhances the directional migration of fibroblasts by increasing both directedness and trajectory speed, suggesting the plausible scenario of cooperation between two physical cues to promote wound healing. When an electric field and shear stress are applied sequentially, migration behavior is affected by the applied stimulation as well as pre-existing stimulating conditions. This microfluidic platform can be utilized to understand other microenvironments such as embryogenesis, angiogenesis and tumor metastasis.</P>

Combinatorial effects of fluid shear stress and electric field on dermal fibroblast migration

Sukhyun Song,Hana Han,Jaemin Kim,Unghyun Ko,Jennifer H. Shin 대한기계학회 2011 대한기계학회 춘추학술대회 Vol.2011 No.4

Review of the Nomenclature of the Retaining Ligaments of the Cheek: Frequently Confused Terminology

서의석,Jennifer Kim Song,오태석,권성일,Tanvaa Tansatit,이주헌 대한성형외과학회 2017 Archives of Plastic Surgery Vol.44 No.4

Since the time of its inception within facial anatomy, wide variability in the terminology as well as the location and extent of retaining ligaments has resulted in confusion over nomenclature. Confusion over nomenclature also arises with regard to the subcutaneous ligamentous attachments, and in the anatomic location and extent described, particularly for zygomatic and masseteric ligaments. Certain historical terms—McGregor’s patch, the platysma auricular ligament, parotid cutaneous ligament, platysma auricular fascia, temporoparotid fasica (Lore’s fascia), anterior platysma-cutaneous ligament, and platysma cutaneous ligament—delineate retaining ligaments of related anatomic structures that have been conceptualized in various ways. Confusion around the masseteric cutaneous ligaments arises from inconsistencies in their reported locations in the literature because the size and location of the parotid gland varies so much, and this affects the relationship between the parotid gland and the fascia of the masseter muscle. For the zygomatic ligaments, there is disagreement over how far they extend, with descriptions varying over whether they extend medially beyond the zygomaticus minor muscle. Even the ‘main’ zygomatic ligament’s denotation may vary depending on which subcutaneous plane is used as a reference for naming it. Recent popularity in procedures using threads or injectables has required not only an accurate understanding of the nomenclature of retaining ligaments, but also of their location and extent. The authors have here summarized each retaining ligament with a survey of the different nomenclature that has been introduced by different authors within the most commonly cited published papers.

Toe Tissue Transfer for Reconstruction of Damaged Digits due to Electrical Burns

Hyung-Do Kim,So-Min Hwang,Kwang-Ryeol Lim,Yong-Hui Jung,Sung-Min Ahn,Jennifer K. Song 대한성형외과학회 2012 Archives of Plastic Surgery Vol.39 No.2

Background Electrical burns are one of the most devastating types of injuries, and can be characterized by the conduction of electric current through the deeper soft tissue such as vessels, nerves, muscles, and bones. For that reason, the extent of an electric burn is very frequently underestimated on initial impression. Methods From July 1999 to June 2006, we performed 15 cases of toe tissue transfer for the reconstruction of finger defects caused by electrical burns. We performed preoperative range of motion exercise, early excision, and coverage of the digital defect with toe tissue transfer. Results We obtained satisfactory results in both functional and aesthetic aspects in all 15cases without specific complications. Static two-point discrimination results in the transferred toe cases ranged from 8 to 11 mm, with an average of 9.5 mm. The mean range of motion of the transferred toe was 20° to 36° in the distal interphalangeal joint, 16° to 45° in the proximal interphalangeal joint, and 15° to 35° in the metacarpophalangeal joint. All of the patients were relatively satisfied with the function and appearance of their new digits. Conclusions The strategic management of electrical injury to the hands can be both challenging and complex. Because the optimal surgical method is free tissue transfer, maintenance of vascular integrity among various physiological changes works as a determining factor for the postoperative outcome following the reconstruction.

Does Pre-season Training Improve Body Composition, Physical Fitness, and Isokinetic Muscle Strength in Female Taekwondo Athletes?

( Jong-kook Song ),( Duk-jae Han ),( Hyun-chul Jung ),( Hyo-jung Kang ),( Myong-won Seo ),( Jennifer Ann Clarke ),( Hyun-bae Kim ) 국제태권도학회 2015 Journal of the International Association for Taekw Vol.2 No.1

The aim of this study was to examine the effects of a 6-week pre-season Taekwondo training program on body composition, physical fitness, and isokinetic muscular strength in female athletes. Twenty-two collegiate female athletes with mean ages ranging from 18.8 to 19.4 years participated. They were divided into four groups according to their weight categories: G1 (≤49 kg), G2 (>49 kg-57 kg), G3 (>57-67 kg), and G4 (>67 kg). The training program included 3 sessions a day, 3 days a week for 6 weeks. Body composition parameters were measured by Dual X-Ray Absorptiometry (DXA). Physical fitness tests including sit and reach, grip strength, sit-ups, standing long jump, 50 m shuttle run, and 20 m multi-stage endurance shuttle run were applied. Isokinetic strength was measured by an isokinetic dynamometer. The extensors and flexors of the knee and trunk were tested at three fixed angular velocities (60°·s^-1, 120°·s^-1, and 240°·s^-1). Significant time x group interaction effects were found for fat tissue (p < 0.05). G4 demonstrated significant decreases in fat tissue (p < 0.01) and percent body fat (p < 0.05) after the training program. However, no significant changes were found for body mass, lean tissue, and bone mineral density in all groups. G1 demonstrated significant improvements on the 50 m shuttle run (p < 0.001) and the multistage endurance run (p < 0.001), while G2 showed significant changes for grip strength (p < 0.01), the 50 m shuttle run (p < 0.01), and the multistage endurance run (p < 0.05). G1 demonstrated significant improvement in left extension (p < 0.0001) and left flexion (p < 0.05) at 60°·s^-1, left flexion (p < 0.001) at 120°·s^-1, and all variables at 240°·s^-1. Significant changes were also found in G2 (left and right extension at 60°·s-1 and 120°·s^-1; right and left flexion at 240°·s^-1), and in G3 (left extension at 60°·s^-1, 120°·s^-1, and 240°·s^-1). However, the training had no effect on trunk isokinetic strength in all groups. In conclusion, this study suggests that a pre-season training program may decrease body fat, while improving agility, endurance, and knee muscular strength in specific weight categories of female Taekwondo athletes. However, this specific type of training should not be considered as an alternative training method for trunk strength in female athletes.

세포기질의 강성이 전단응력으로 유도된 혈관내피세포 운동성에 미치는 영향에 대한 연구

송석현(Sukhyun Song),김미나(Mina Kim),김현유(Hyeonyu Kim),신현정(Jennifer H. Shin) 대한기계학회 2009 대한기계학회 춘추학술대회 Vol.2009 No.11

Vascular endothelial cell (VEC) forms a monolayer on the smooth muscle cell layer of blood vessel. Thus, substrate stiffness should be considered as one of the most important element in mimicking the physiological condition of VECs. In this research, the effect of substrate stiffness on shear stress induced cellular motility was investigated. VECs are seeded on polydimethylsiloxane (PDMS) substrate with controlled stiffness, ranging from 1.78 ㎫ to 48 ㎪. Shear stress system based on parallel plate flow chamber is utilized to apply laminar shear stress of 15 dyne/㎠. In order to explain the detailed mechanism of cellular migration, expression and distribution of cytoskeleton related pathway consisting RhoA, Rac1 and Cdc42 are examined. In addition, the regulation of syndecan-4 is observed as a mechanoreceptor. Results reveal that there is an optimal stiffness in maximizing the cell motility.