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Lamichhane, Janardan,Lee, Hei Chan,Liou, Kwankyoung,Kim, Chun Gyu,Sohng, Jae Kyoung 한국공업화학회 2004 응용화학 Vol.8 No.2
Rubradirin, as ansamycin antibiotic has been isolated from Streptomyces achromogenes va, rubradiris NRRL 3061. It consists of four distinct structural moieties. Amide bond present in th( structure of rubradirin plays an important role as a linkage between the coumarin ring an( didipicolinic acid moiety of the rubradirin. We disrupted the amide bond present in the structure o rubradirin and then analyzed the production of rubradirin and its mutated product with respect to the wild strain. It was observed that the formation of rubradirin could not take place in mutant. Thy rubransarol containing fractions and the native rubradirin were detected by HPLC.
Kim, Chun-Gyu,Lamichhane, Janardan,Song, Kwang-Il,Nguyen, Van D.,Kim, Dong-Hee,Jeong, Tae-Sung,Kang, Sung-Ho,Kim, Kyoung-Wook,Maharjan, Jyoti,Hong, Young-Soo,Kang, Jae Seon,Yoo, Jin-Cheol,Lee, Jung-Jo Springer-Verlag 2008 Archives of microbiology Vol.189 No.5
<P>The four overlapping cosmids from the rubradirin producer, Streptomyces achromogenes var rubradiris NRRL 3061, have 58 ORFs within a 105.6 kb fragment. These ORFs harbored essential genes responsible for the formation and attachment of four distinct moieties, along with the genes associated with regulatory, resistance, and transport functions. The PKS (rubA) and glycosyltransferase (rubG2) genes were disrupted in order to demonstrate a complete elimination of rubradirin production. The rubradirin biosynthetic pathway was proposed based on the putative functions of the gene products, the functional identification of sugar genes, and the mutant strains.</P>
( Amit Kumar Jha ),( Janardan Lamichhane ),( Jae Kyung Sohng ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.1
Structurally, herboxidiene contains the tetrahydropyran acetic acid moiety and a side chain including a conjugated diene, and has been isolated from Streptomyces chromofuscus ATCC 49982. Its production was significantly elevated nearly 13.5-fold (0.74 g/l) in a medium supplemented with glycerol (medium No. 6A6), and was more efficacious (1.08 g/l; 19.8-fold) in fed-batch fermentation at 36 h in medium No. 6A6, from Streptomyces chromofuscus. For further enhancement, regulatory genes metK1-sp and afsR-sp from Streptomyces peucetius were overexpressed using an expression vector, pIBR25, and similarly ACCase from Streptomyces coelicolor and two genes, metK1-sp and afsR-sp, were also overexpressed using an integration vector, pSET152, under the control of the strong ermE promoter in Streptomyces chromofuscus. Only the recombinant strains S. chromofuscus SIBR, S. chromofuscus GIBR, and S. chromofuscus AFS produced more herboxidiene than the parental strain in optimized medium No. 6A6 with an increment of 1.32-fold (0.976 g/l), 3.85-fold (2.849 g/l), and 1.7-fold(1.258 g/l) respectively.