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Effect of Cyclic AMP on the Two Promoters of Escherichia coli Thioredoxin Gene
Sa, Jae-Hoon,Fuchs, James A.,Lim, Chang-Jin Korean Society for Biochemistry and Molecular Biol 1997 Journal of biochemistry and molecular biology Vol.30 No.5
Thioredoxin is a multi-functional protein which is ubiquitous in microorganisms, animals and plants. Previously, expression of the E. coli thioredoxin gene was found to be negatively regulated by cAMP. In the present study, the effect of cAMP on two separate promoters of the E. coli thioredoxin gene was investigated. Cyclic AMP had a repressible effect on P1 and P1P2 promoter activity of the constructs. This effect was also observed in the cya strain. The P2 promoter construct gave very high -galactosidase activity, and its expression was not affected by exogenous cAMP. It was assumed that a cis-acting negative element, probably the cAMP-CRP binding site, might have been deleted in the P1 promoter construct. Repression of the thioredoxin gene expression by cAMP appeared to be independent of ppGpp.
Effect of Cyclic AMP on the Two Promoters of Escherichia coli Thioredoxin Gene
Lim,Chang-Jin,Fuchs, James A.,Sa,Jae-Hoon The Korea Science and Technology Center 1997 BMB Reports Vol.30 No.5
Thioredoxin is a multi-functional protein which is ubiquitous in microorganisms, animals and plants. Previously, expression of the E. coli thioredoxin gene was found to be negatively regulated by cAMP. In the present study, the effect of cAMP on two separate promoters of the E. coli thioredoxin gene was investigated. Cyclic AMP had a repressible effect on P1 and P1P2 promoter activity of the constructs. This effect was also observed in the cya strain. The P2 promoter construct gave very high β-galactosidase activity, and its expression was not affected by exogenous cAMP. It was assumed that a cis-acting negative element, probably the cAMP-CRP binding site, might have been deleted in the P1 promoter construct. Repression of the thioredoxin gene expression by cAMP appeared to be independent of ppGpp.
Effect of Cyclic AMP on the Two Promoters of Escherichia coli Thioredoxin Gene
( Jae Hoon Sa,James A . Fuchs,Chang Jin Lim ) 생화학분자생물학회 1997 BMB Reports Vol.30 No.5
Thioredoxin is a multi-functional protein which is ubiquitous in microorganisms. animals and plants. Previously. expression of the E. coli thioredoxin gene was found to be negatively regulated by cAMP. In the present study. the effect of cAMP on two separate promoters of the E. coli thioredoxin gene was investigated. Cyclic AMP had a repressible effect on P1 and P1P2 promoter activity of the constructs. This effect was also observed in the cya strain. The P2 promoter construct gave very high β-galactosidase activity. and its expression was not affected by exogenous cAMP It was assumed that a cis-acting negative element. probably the cAMP-CRP binding site, might have been deleted in the P1 promoter construct. Repression of the thioredoxin gene expression by cAMP appeared to be independent of ppGpp.
Effect of cyclic AMP on the Two Promoters of Escgerichia coli Thioredoxin Gene
Jae-Hoon Sa,James A. Fuchs,Chang-Jin Lim 강원대학교 기초과학연구소 1997 기초과학연구 Vol.8 No.-
Thioredoxin is a multi-functional protein which is ubiquitous in microorganisms, animals and plants. Previously, expression of the E. coli thioredoxin gene was found to be negatively regulated by cAMP. In the present study. the effect of cAMP on two separate promoters of the E. coli thioredoxin gene was investigated. Cyclic AMP had a repressible effect on P1 and P1P2 promoter activity of the constructs. This effect was also observed in the cya strain. The P2 promoter construct gave very high β-galactosidase activity, and its expression was not affected by exogenous cAMP. It was assumed that a cis -acting negative element, probably the cAMP-CRP binding site, might have been deleted in the P1 promoter construct. Repression of the thioredoxin gene expression by cAMP appeared to be independent of ppGpp.
Experssion of the Escherichia coli Thioredoxin Gene is Negatively Regulated
Jae-Hoon Sa,Mi Ae Namgung,chung-Jin Lem,James A Fuchs 강원대학교 기초과학연구소 1997 기초과학연구 Vol.8 No.-
Regulation of the Escherichia coli thioredoxin gene (trxA) was studied using trxA-lac translational fusion constructed in the vector pMC1403. Synthes of β of galactosidase from the trxA-Iac fusion was found to be repressed in the presence of lactose. A switch of carbon source from glucose to lactose and an addition of cyclic AMP (cAMP) caused a decrease in βgalactosidase synthesis from the trxA-lac fusion. The repression effect of exogenous cAMP was not observed in the crp mutant strain. The β-galactosidase synthesis from the trxA-lac fusion lacking a plausible cAMP-CRP binding site was not lowered in the presence of lactose or in the addition of cAMP. Expression of the chromosomal trxA gene was reduced by exogenous cAMP. These findings indicate that the expression of the trxA gene is controlled by cAMP in a negative manner