Intravitreal Tanibirumab, a Fully Human Monoclonal Antibody Against Vascular Endothelial Growth Factor Receptor 2, Partially Suppresses and Regresses Laser-Induced Choroidal Neovascularization in a Rat Model

Kim, Jaeryung,Kim, Tae Eun,Kim, Ju-A,Yun, Ji-Hyun,Sohn, Seongsoo,Shim, Sang Ryeol,Lee, Sang Hoon,Kim, Sang Jin MARY ANN LIEBERT, INC 2014 JOURNAL OF OCULAR PHARMACOLOGY AND THERAPEUTICS Vol.30 No.10

<P>Purpose: The study investigated the effect of intravitreally administered tanibirumab, a fully human monoclonal antibody against vascular endothelial growth factor receptor 2, in a rat model of laser-induced choroidal neovascularization (CNV). Methods: CNV was induced by laser photocoagulation on day 0 in the eyes of Brown Norway rats. Intravitreal injection of tanibirumab or phosphate-buffered saline (PBS) was done on day 0 (prevention arm) or day 7 (treatment arm). Seven days after injection, the eyes were enucleated and retinal pigment epithelium-choroid-sclera flat mounts were prepared. Areas of CNV were determined in the flat mounts using tetramethylrhodamine isothiocyanate Bandeiraea simplicifolia (BS) isolectin labeling and intravenously administered fluorescein isothiocyanate-dextran and quantified using an image analysis program. Results: In the prevention arm, the mean area of CNV measured by BS isolectin labeling was reduced by 28.2% and 53.9% in tanibirumab-treated eyes (20 and 60 mu g, respectively) compared with PBS-treated control eyes on day 7 (P=0.038 and P<0.001, respectively). In the treatment arm, the mean area of CNV measured by BS isolectin labeling was reduced by 28.7% and 46.0% in tanibirumab-treated eyes (20 and 60 mu g, respectively) compared with PBS-treated control eyes on day 14 (P=0.048 and P<0.001, respectively). Conclusions: Intravitreally administered tanibirumab partially suppressed the formation of new CNV and partially regressed preformed laser-induced CNV in the rat model. Tanibirumab may be a feasible treatment for CNV associated with age-related macular degeneration or other causes.</P>

YAP/TAZ regulates sprouting angiogenesis and vascular barrier maturation

Kim, Jongshin,Kim, Yoo Hyung,Kim, Jaeryung,Park, Do Young,Bae, Hosung,Lee, Da-Hye,Kim, Kyun Hoo,Hong, Seon Pyo,Jang, Seung Pil,Kubota, Yoshiaki American Society for Clinical Investigation 2017 The Journal of clinical investigation Vol.127 No.9

The correlation between retinal sensitivity assessed by microperimetry and contrast sensitivity in diabetic macular oedema

Kim, Young Ho,Yun, Cheolmin,Kim, Jee Taek,Kim, Seong-Woo,Oh, Jaeryung,Huh, Kuhl British Medical Association 2014 British journal of ophthalmology Vol.98 No.12

<P><B>Aim</B></P><P>To investigate the relationship between contrast sensitivity (CS) and retinal sensitivity (RS) assessed by microperimetry (MP) in diabetic retinopathy (DR) with clinically significant macular oedema (CSME).</P><P><B>Methods</B></P><P>A retrospective study was performed with 35 eyes of 35 patients with DR and CSME. Retinal thickness (RT) and MP were tested with the spectral SD-optical coherence topography/scanning laser ophthalmoscope system. Mean central RT at the fovea centre's 1 mm zone (CRT) and at the fixation centre's 1 mm zone (FCRT) was measured. RS was tested at the fixation centre, within 2° and 4° areas. CS was measured with six target sizes (6.3°, 4.0°, 2.5°, 1.6°, 1.0°, 0.64°) with a contrast glare tester.</P><P><B>Results</B></P><P>The mean CRT and FCRT were 344.3±136.2 and 359.9±135.5 μm, respectively. Mean log CSs (−log10) with the six target sizes ranged from 0.19 to 1.32. The mean RS at the fixation centre, within 2°, and within 4° area were 8.51±4.81 dB, 8.58±3.88 dB and 9.22±3.56 dB, respectively. RS at all tested areas were significantly correlated to log CS with all target sizes (range, r=0.366–0.755; p=0.0001–0.030). CRT and FCRT were not significantly correlated to log CS or RS.</P><P><B>Conclusions</B></P><P>CS and RS showed moderately significant correlations in CSME. However, neither CS nor RS was correlated with RT in patients with CSME. It could be that CS and MP are complementary to each other and are useful tools in the evaluation of functional vision.</P>

Retinal Vein Occlusion is Associated with Low Blood High-Density Lipoprotein Cholesterol: A Nationwide Cohort Study

Kim, Jaeryung,Lim, Dong Hui,Han, Kyungdo,Kang, Se Woong,Ham, Don-Il,Kim, Sang Jin,Chung, Tae-Young Elsevier 2019 American journal of ophthalmology Vol.205 No.-

<P><B>Purpose</B></P> <P>To investigate association between the development of retinal vein occlusion (RVO) and blood high-density lipoprotein cholesterol (HDL-C).</P> <P><B>Design</B></P> <P>A retrospective, nationwide, population-based cohort study.</P> <P><B>Methods</B></P> <P>This study was set in the Republic of Korea and included 23,149,403 people ≥20 years of age who underwent the Korean National Health Screening Program examination between January 2009 and December 2012. Among them, the RVO group was composed of patients with an initial diagnosis of RVO made between 2009 and 2015 (n = 117,639). The earliest claim with an RVO diagnostic code was considered as the incident time. The predictive value of HDL-C level for RVO was analyzed using hazard ratios. The primary outcome measure was the incident cases of RVO.</P> <P><B>Results</B></P> <P>Subjects with RVO were generally older; had high body mass index, waist circumference, fasting blood glucose, blood pressure, total and low-density lipoprotein cholesterol, and triglyceride values, and low glomerular filtration rate and HDL-C values; and were more likely to experience diabetes mellitus and hypertension compared with the non-RVO group. The fully adjusted hazard ratio of RVO was 1.12 (95% confidence interval 1.10–1.14) in the lowest quartile of HDL-C versus in the highest quartile. The association between the development of RVO and HDL-C was higher those with a younger age, male sex, current smoking habit, diabetes mellitus, and hypercholesterolemia. In addition, we observed a significant synergistic effect of low HDL-C level with obesity and hypertension.</P> <P><B>Conclusion</B></P> <P>This is the first nationwide population-based epidemiologic study evaluating the association between HDL-C level and the risk of RVO development. A significant association between low HDL-C and RVO development was found.</P>

COMBINED INTRAVITREAL BEVACIZUMAB INJECTION AND ZONE I SPARING LASER PHOTOCOAGULATION IN PATIENTS WITH ZONE I RETINOPATHY OF PREMATURITY

Kim, Jaeryung,Kim, Sang Jin,Chang, Yun Sil,Park, Won Soon by Ophthalmic Communications Society, Inc. 2014 Retina Vol.34 No.1

PURPOSE:: To investigate the anatomical outcome of combined intravitreal bevacizumab injection and Zone I sparing laser ablation in patients with Type 1 retinopathy of prematurity in Zone I. METHODS:: The medical records of consecutive 18 eyes of 10 infants, who underwent combined intravitreal bevacizumab (0.25 mg) injection and Zone I sparing laser ablation for the treatment of Type 1 retinopathy of prematurity in Zone I, were retrospectively reviewed. Laser photocoagulation was performed on the avascular retina anterior to the margin of Zone I extending to the ora serrata. Anatomical outcomes including progression to stage 4/5, macular changes, and vitreous organization were reviewed. RESULTS:: The mean gestational age at birth and the birth weight of included patients were 24.0 weeks and 628 g, respectively. The timing of bevacizumab injection ranged from postmenstrual age 33 to 35 weeks (mean, 34.3 weeks). Postmenstrual age at last follow-up ranged from 74 to 107 weeks (mean, 83.6 weeks). All 18 eyes demonstrated prompt regression of neovascular pathology and plus disease without recurrence. Previously avascular Zone I retina was vascularized in all eyes after the treatment. All eyes showed excellent anatomical outcome with intact macula, but one eye showed mild vitreous organization above the vascular/avascular junction. CONCLUSION:: Combined intravitreal bevacizumab injection and Zone I sparing laser ablation for Type 1 retinopathy of prematurity in Zone I seem to be effective treatment options. Possible advantages include lower dose of anti–vascular endothelial growth factor, less recurrence than monotherapy, and preservation of central visual field.

결절맥락막혈관병증에서 인도시아닌그린 혈관조영술과 빛간섭단층혈관조영술의 비교

김종현(Jonghyun Kim),안소민(So Min Ahn),윤철민(Cheolmin Yun),김성우(Seong-Woo Kim),오재령(Jaeryung Oh) 대한안과학회 2021 대한안과학회지 Vol.62 No.9

목적: 결절맥락막혈관병증에서 인도시아닌그린혈관조영술과 빛간섭단층혈관조영술의 비교를 통해 진단적 가치를 평가하고 차이가 발생하는 요인을 알아보고자 한다. 대상과 방법: 43명 환자들의 인도시아닌그린혈관조영술과 빛간섭단층혈관조영술검사에서 결절 개수와 크기, 분지혈관망 유무의 일치율과 크기를 비교하였다. 두 검사에서 결절 개수 일치 여부에 따라 그룹 1은 두 검사에서 결절 개수가 같은 군으로, 그룹 2는 개수가 다른 군으로 분류하였다. 결과: 결절의 개수와 면적은 인도시아닌그린혈관조영술에서는 평균 1.47 ± 0.83개, 0.27 ± 0.42 mm2, 빛간섭단층혈관조영술검사에서는 평균 1.07 ± 0.91개, 0.17 ± 0.15 mm2로 유의미한 차이를 보였다(각각 p<0.001, p=0.023). 분지혈관망은 인도시아닌그린혈관조영술로 33안(76.7%)에서 평균 면적 3.61 ± 2.59 mm2, 빛간섭단층혈관조영술검사로 29안(67.4%)에서 평균 면적 2.74 ± 2.76 mm2로 측정되어 유의미한 차이를 보였다(p=0.002). 중심망막두께와 중심맥락막두께는 그룹 2에서 유의미하게 두꺼웠다(각각 p<0.001). 그룹 2에서 망막하액(p=0.009), 망막하출혈(p=0.035)이 유의미하게 많이 발견되었고, 결절의 높이(p=0.022), 결절의 폭(p=0.042)이 유의미하게 크게 측정되었다. 결론: 빛간섭단층혈관조영술은 결절맥락막혈관병증을 진단하는 데 인도시아닌그린혈관조영술과 차이를 보이나 진단에 도움을 줄 수 있는 보조적인 진단 방법이며, 망막하액과 망막하출혈이 존재하고 결절의 높이와 폭이 큰 경우 결절맥락막혈관병증 진단에 영향을 미칠 수 있다. Purpose: To assess the diagnostic value of optical coherence tomography angiography (OCTA), and the factors affecting the diagnosis of polypoidal choroidal vasculopathy (PCV) by OCTA and indocyanine green angiography (ICGA). Methods: The numbers and area of polyps, and the presence and area of a branched vascular network (BVN) as revealed by ICGA and OCTA, were retrospectively analyzed in 43 patients with active PCV. The patients were divided into two groups according to whether the number of polyps matched between the two methods: group 1, equal number of polyps revealed by ICGA and OCTA; group 2, different number of polyps revealed by ICGA and OCTA. Results: In 43 PCV patients, the total number of polyps was 1.47 ± 0.83 in ICGA and 1.07 ± 0.91 in OCTA (p < 0.001), and the polyp area was 0.27 ± 0.42 mm2 in ICGA and 0.17 ± 0.15 mm2 in OCTA (p = 0.023). BVN was found in 33 eyes (76.7%) by ICGA and 29 eyes (67.4%) by OCTA (p < 0.001). The BVN area was 3.61 ± 2.59 mm2 in ICGA and 2.74 ± 2.76 mm2 in OCTA (p = 0.002). Central retinal thickness and central choroidal thickness were significantly greater in group 2 than group 1 (p < 0.001, respectively). Subretinal fluid (SRF) (p = 0.009) and subretinal hemorrhage (SRH) (p = 0.005) were significantly more prevalent in group 2 than group 1. Polyp height (p = 0.022) and diameter (p = 0.042) were significantly greater in group 2 than group 1. Conclusions: OCTA is a supplementary diagnostic technique for detecting PCV. The presence of SRF and SHR, and large polyp height and diameter, were associated with the polyp detection rate of OCTA for PCV.

점안약제에 사용된 여과 장치의 세균여과 효과

김균형,오재령,김준헌,김효명,Kyun Hyung Kim,Jaeryung Oh Ph,D,Joon-Heon Kim,Hyo-Myung Kim Ph,D 대한안과학회 2007 대한안과학회지 Vol.48 No.10

Purpose: A filtering system (ABAK system, Thea, France) was developed and has been used to prevent the abuse of preservatives and to decrease the complications that may result from them. However, the bacteria filtering effect of the system has not been reported yet. In this study, we attempt to verify its efficacy. Methods: Staphylococcus epidermidis was diluted to two different concentrations, 107 and 105 CFU (Colony-Forming-Unit)/ml. To determine the inward-filtering effect of the system (reverse direction), 0.5 ml of each bacterial concentration was aspirated through the ABAK system, and the solutions that filtered through were cultivated. The results were compared with the controls in which the same amounts of bacterial solutions were dropped from a dropper by squeezing the bottle. For the outward-effect (forward direction), 1ml of bacterial solution from each concentration was put into the bottle with a syringe. Solutions were re-collected by filtering them out through the ABAK system by squeezing the bottle and also by aspirating them from the bottle with a syringe. Both solutions were cultivated, and the results were compared. Each test was repeated 5 times. Results: In control solutions that did not pass through the filter, bacteria were cultivated consistently in repeated tests. However, bacteria were not cultivated in solutions that had passed the filter in both concentrations and in both directions. Conclusions: Filtering systems prevent solutions from contamination, and even if the bottles are polluted with Staphylococcus epidermidis initially, the bacteria would be filtered out. The effectiveness of the filtering systems was also demonstrated in high bacterial concentrations.

COMPARISON OF CHOROIDAL THICKNESS AMONG PATIENTS WITH HEALTHY EYES, EARLY AGE-RELATED MACULOPATHY, NEOVASCULAR AGE-RELATED MACULAR DEGENERATION, CENTRAL SEROUS CHORIORETINOPATHY, AND POLYPOIDAL CHOROIDAL VASCULOPATHY

Kim, Seong-Woo,Oh, Jaeryung,Kwon, Soon-Sun,Yoo, Junho,Huh, Kuhl The Ophthalmic Communications Society, Inc. 2011 Retina Vol.31 No.9

PURPOSE:: To compare choroidal thicknesses among eyes with early age-related maculopathy (ARM), neovascular age-related macular degeneration, polypoidal choroidal vasculopathy, and central serous chorioretinopathy. METHODS:: Patients with age-related maculopathy (37 eyes), neovascular age-related macular degeneration (24 eyes), polypoidal choroidal vasculopathy (12 eyes), and central serous chorioretinopathy (31 eyes) underwent spectral-domain optical coherence tomography evaluations using a choroid scanning protocol. A horizontal linear section comprising 50 averaged scans was obtained of each macula. The choroidal thickness was measured from the outer border of the retinal pigment epithelium to the inner scleral border. Twenty-nine subjects with healthy eyes served as a control group. Analysis of covariance tests were performed to evaluate the effects of various diagnoses on choroidal thickness after removal of variance (covariates = gender, age, and refractive error). RESULTS:: Among the different covariates, age was associated with choroidal thickness (fovea: F = 12.067, P = 0.001). After controlling for age differences, the choroid was thicker in polypoidal choroidal vasculopathy (319.92 ± 68.66 μm) and central serous chorioretinopathy (367.81 ± 105.56 μm) patients than in controls (241.97 ± 66.37 μm) and age-related maculopathy patients (186.62 ± 64.02 μm). However, there were no significant differences in mean choroidal thickness between neovascular age-related macular degeneration (226.46 ± 102.87 μm) and any of the other diagnoses. CONCLUSION:: The choroid was thicker in eyes with polypoidal choroidal vasculopathy or central serous chorioretinopathy than in control or age-related maculopathy groups.

Evaluation of the Safety and Efficacy of Selective Retina Therapy Laser Treatment in Patients with Central Serous Chorioretinopathy

Jaeryung Oh,Chang Ki Yoon,Bo Hee Kim,Hyeong Gon Yu 대한안과학회 2021 Korean Journal of Ophthalmology Vol.35 No.1

Purpose: To assess the safety and efficacy of selective retina therapy (SRT) using a Q-switched neodymium-doped yttriumlithium fluoride laser with feedback systems in patients with idiopathic central serous chorioretinopathy (CSC). Methods: This randomized clinical trial enrolled patients having at least 3-month symptom of CSC. From month 3 visit, allsubjects in both groups were eligible for SRT retreatment if they showed persistent or recurrent subretinal fluid (SRF). The primaryoutcome was complete resolution of SRF by optical coherence tomography at 3 months after treatment. The secondaryoutcomes were changes in SRF, central macular thickness (CMT) and best-corrected visual acuity at the 1-, 3-, and 6-monthexaminations. Results: Sixty-eight CSC patients were included (SRT, 31; control, 37). After 1 and 3 months, complete resolution of SRF wasachieved in 25.8% and 54.8% of SRT group and 17.6% and 35.1% of controls. The differences were not statistically significant(p = 0.424 and p = 0.142, respectively). However, mixed model for repeated measures analyses showed that the reduction ofSRF and CMT were observed earlier in SRT group than in the sham group (least squares mean difference, -59.7 μm; 95% confidenceinterval, -98.2 to -21.2; p = 0.0029; least squares mean difference -67.0 μm; 95% confidence interval, -104.8 to -29.2;p = 0.0007, respectively). Significant reduction of SRF (≥50% reduction from baseline) was more frequently observed in SRTgroup (80.6%) than the sham group (44.1%) at month 1 (p = 0.007). Early reduction of SRF and CMT was more abundant inSRT group with symptom duration less than 6 months. Treatment related serious adverse events were not observed. Conclusions: SRT using a Q-switched neodymium-doped yttrium lithium fluoride laser with feedback system was safe in thistrial and effective for early resolution of SRF in the CSC patients. Early intervention with SRT can be a safe alternative for patientswith acute symptomatic CSC.

Correlations among various functional and morphological tests in resolved central serous chorioretinopathy.

Kim, Seong-Woo,Oh, Jaeryung,Huh, Kuhl British Medical Association 2012 British journal of ophthalmology Vol.96 No.3

<P>To find the explanatory parameters for best corrected visual acuity (BCVA) and microperimetry (MP) in resolved central serous chorioretinopathy.</P>