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Characteristics of Cell Lines Established from Human Gastric Carcinoma
Park, J G,Oie, H K,LaRocca, R V,Johnson, B E,Bang, Y J,Kim, J P,Israel, M A,Gazdar, A F 한국유전학회 1988 Genes & Genomics Vol.10 No.4
Four gastric carcinoma cell lines were established from one primary and three metastatic tumors. All lines expressed human form of the 4 enzyme tested and were free of contamination with mycoplasma or murine viruses. Population doubling times ranged from 26-47 h. Cloning efficiency ranged from 1.9-27%. Three of 4 cultures displayed both adherent and floating subpopulations. Cell line, NCI-N87, grew as adherent monolayers. We examined for expression of DDC, an enzyme characteristic of NE cells. Relatively high levels of enzyme activity (>10units/㎎ protein; 1unit = 1nM CO2/m) was detected in 2 lines but all lines didn't show cytoplasmic dense core ("neurosecretory") granules, mRNA expression of chromogranin A, and didn't reacted with natural killer cell antigens, Leu-7 and NKH-1. Double minute chromosomes(DMs) were found in all 4 cell lines. Homogeneously staining regions(HSRs) were present in the minor subline of SNU-16. One line, SNU-16, has c-myc gene amplification. N-myc, L-myc, myb and EGF-R gene were not amplified. All of 4 lines expressed c-myc mRNA and erb B mRNA. N-myc, L-myc, v-sis, IGF-2 and GRP were not expressed. In this report we describe the establishment and characterization of 4 continuous cell lines derived from human primary and metastatic gastric carcinomas. However a modest number of gastric carcinoma lines have been described, our report describes three interesting or unique features, including expression and secretion of high concentration of 3 gastrointestinal cell associated antigens; high frequency of expression of the NE cell marker DDC; and possibly unique line with c-myc gene amplification and subline having HSRs at 4 separate chromosomal locations.
LEE, J.-S.,KIM, H.-S.,HAHM, K.-B.,SOHN, M.-W.,YOO, M.,JOHNSON, J. A,SURH, Y.-J. Wiley (Blackwell Publishing) 2007 Annals of the New York Academy of Sciences Vol.1095 No.1
<P>The Helicobacter pylori were identified by Marshall and Warren in 1984. H. pylori survive in the forbidding harsh acid environment of the stomach and duodenum by hiding in the mucus layer and neutralizing gastric acid in its local surrounding environment. Multiple lines of evidence suggest that H. pylori infection is one of the primary causes of gastritis and peptic ulcer, which are provoked by oxidative stress and inflammation. More than 50% of the world's population is infected by this bacterium. The H. pylori-induced inflammation has been implicated in the pathogenesis and progression of gastric cancer. DA-6034 (7-carboxymethyloxy-3',4',5-trimethoxy flavone) is a synthetic flavonoid known to possess anti-inflammatory activity. It has been reported that oral administration of DA-6034 suppresses the inflammatory bowel disease (IBD) in animal models. In this article, we attempted to examine the effect of DA-6034 on H. pylori-induced inflammation in human gastric cancer (AGS) cells by targeting NF-kappaB and extracellular signal-regulated kinase (ERK), a representative MAPK.</P>
Choi, S.H.,Park, S.K.,Johnson, B.J.,Chung, K.Y.,Choi, C.W.,Kim, K. H.,Kim, W.Y.,Smith, S.B. Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.3
We previously demonstrated that bovine subcutaneous preadipocytes promote adipogenic gene expression in muscle satellite cells in a co-culture system. Herein we hypothesize that saturated fatty acids would promote adipogenic/lipogenic gene expression, whereas mono- and polyunsaturated fatty acids would have the opposite effect. Bovine semimembranosus satellite cells (BSC) and intramuscular preadipocytes (IPA) were isolated from crossbred steers and cultured with 10% fetal bovine serum (FBS)/Dulbecco's Modified Eagle Medium (DMEM) and 1% antibiotics during the 3-d proliferation period. After proliferation, cells were treated for 3 d with 3% horse serum/DMEM (BSC) or 5% FBS/DMEM (IPA) with antibiotics. Media also contained $10{\mu}g/mL$ insulin and $10{\mu}g/mL$ pioglitazone. Subsequently, differentiating BSC and IPA were cultured in their respective media with $40{\mu}M$ palmitic, stearic, oleic, or linoleic acid for 4 d. Finally, BSC and IPA were single- or co-cultured for an additional 2 h. All fatty acid treatments increased (p = 0.001) carnitine palmitoyltransferase-1 beta ($CPT1{\beta}$) gene expression, but the increase in $CPT1{\beta}$ gene expression was especially pronounced in IPA incubated with palmitic and stearic acid (6- to 17-fold increases). Oleic and linoleic acid decreased (p = 0.001) stearoyl-CoA desaturase (SCD) gene expression over 80% in both BSC and IPA. Conversely, palmitic and stearic acid increased SCD gene expression three fold in co-cultured in IPA, and stearic acid increased $AMPK{\alpha}$ gene expression in single- and co-cultured BSC and IPA. Consistent with our hypothesis, saturated fatty acids, especially stearic acid, promoted adipogenic and lipogenic gene expression, whereas unsaturated fatty acids decreased expression of those genes associated with fatty acid metabolism.
S. H. Choi,박성권,B.J. Johnson,K.Y. Chung,C.W. Choi,김경훈,W.Y. Kim,S.B. Smith 아세아·태평양축산학회 2015 Animal Bioscience Vol.28 No.3
We previously demonstrated that bovine subcutaneous preadipocytes promote adipogenic gene expression in muscle satellite cells in a co-culture system. Herein we hypothesize that saturated fatty acids would promote adipogenic/lipogenic gene expression, whereas mono- and polyunsaturated fatty acids would have the opposite effect. Bovine semimembranosus satellite cells (BSC) and intramuscular preadipocytes (IPA) were isolated from crossbred steers and cultured with 10% fetal bovine serum (FBS)/ Dulbecco’s Modified Eagle Medium (DMEM) and 1% antibiotics during the 3-d proliferation period. After proliferation, cells were treated for 3 d with 3% horse serum/DMEM (BSC) or 5% FBS/DMEM (IPA) with antibiotics. Media also contained 10 μg/mL insulin and 10 μg/mL pioglitazone. Subsequently, differentiating BSC and IPA were cultured in their respective media with 40 μM palmitic, stearic, oleic, or linoleic acid for 4 d. Finally, BSC and IPA were single- or co-cultured for an additional 2 h. All fatty acid treatments increased (p = 0.001) carnitine palmitoyltransferase-1 beta (CPT1β) gene expression, but the increase in CPT1β gene expression was especially pronounced in IPA incubated with palmitic and stearic acid (6- to 17- fold increases). Oleic and linoleic acid decreased (p = 0.001) stearoyl-CoA desaturase (SCD) gene expression over 80% in both BSC and IPA. Conversely, palmitic and stearic acid increased SCD gene expression three fold in co-cultured in IPA, and stearic acid increased AMPKα gene expression in single- and co-cultured BSC and IPA. Consistent with our hypothesis, saturated fatty acids, especially stearic acid, promoted adipogenic and lipogenic gene expression, whereas unsaturated fatty acids decreased expression of those genes associated with fatty acid metabolism.
Epigenetic regulation of RNA polymerase III transcription in early breast tumorigenesis
Park, J-L,Lee, Y-S,Song, M-J,Hong, S-H,Ahn, J-H,Seo, E-H,Shin, S-P,Lee, S-J,Johnson, B H,Stampfer, M R,Kim, H-P,Kim, S-Y,Lee, Y S The Author(s) 2017 Oncogene Vol.36 No.49
RNA polymerase III (Pol III) transcribes medium-sized non-coding RNAs (collectively termed Pol III genes). Emerging diverse roles of Pol III genes suggest that individual Pol III genes are exquisitely regulated by transcription and epigenetic factors. Here we report global Pol III expression/methylation profiles and molecular mechanisms of Pol III regulation that have not been as extensively studied, using nc886 as a representative Pol III gene. In a human mammary epithelial cell system that recapitulates early breast tumorigenesis, the fraction of actively transcribed Pol III genes increases reaching a plateau during immortalization. Hyper-methylation of Pol III genes inhibits Pol III binding to DNA via inducing repressed chromatin and is a determinant for the Pol III repertoire. When Pol III genes are hypo-methylated, MYC amplifies their transcription, regardless of its recognition DNA motif. Thus, Pol III expression during tumorigenesis is delineated by methylation and magnified by MYC.
Control of Spin Precession in a Spin-Injected Field Effect Transistor
Koo, H. C.,Kwon, J. H.,Eom, J.,Chang, J.,Han, S. H.,Johnson, M. American Association for the Advancement of Scienc 2009 Science Vol.325 No.5947
<P>Spintronics increases the functionality of information processing while seeking to overcome some of the limitations of conventional electronics. The spin-injected field effect transistor, a lateral semiconducting channel with two ferromagnetic electrodes, lies at the foundation of spintronics research. We demonstrated a spin-injected field effect transistor in a high-mobility InAs heterostructure with empirically calibrated electrical injection and detection of ballistic spin-polarized electrons. We observed and fit to theory an oscillatory channel conductance as a function of monotonically increasing gate voltage.</P>
Spatiotemporal Evolution of the Primary Glioblastoma Genome
Kim, J.,Lee, I.H.,Cho, H.,Park, C.K.,Jung, Y.S.,Kim, Y.,Nam, S.,Kim, B.,Johnson, Mark D.,Kong, D.S.,Seol, H.,Lee, J.I.,Joo, K.,Yoon, Y.,Park, W.Y.,Lee, J.,Park, Peter J.,Nam, D.H. Cell Press 2015 CANCER CELL Vol. No.
Tumor recurrence following treatment is the major cause of mortality for glioblastoma multiforme (GBM) patients. Thus, insights on the evolutionary process at recurrence are critical for improved patient care. Here, we describe our genomic analyses of the initial and recurrent tumor specimens from each of 38 GBM patients. A substantial divergence in the landscape of driver alterations was associated with distant appearance of a recurrent tumor from the initial tumor, suggesting that the genomic profile of the initial tumor can mislead targeted therapies for the distally recurred tumor. In addition, in contrast to IDH1-mutated gliomas, IDH1-wild-type primary GBMs rarely developed hypermutation following temozolomide (TMZ) treatment, indicating low risk for TMZ-induced hypermutation for these tumors under the standard regimen.