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Rodriguez,Camille Ann M,Bayron,Jasmin Francesca J. Gomeri,Ira Nicole A. Tabuyo,Jimford U 한국문화관광학회 2021 문화관광연구 Vol.23 No.2
The research is about the Action Plan of Community-Based Ecotourism in Brgy. Alas-As in San Nicolas, Batangas. The study aimed to provide possible recommendations and solutions to build more opportunities for the improvement of community-based ecotourism in Brgy. Alas-As in San Nicolas, Batangas. The Brgy. Alas-As in San Nicolas, Batangas is the leading community that embodies community-based ecotourism. The researchers used ASEAN Community-Based Tourism (CBT) Standard as framework for the study. The research was to apply the ASEAN Community-Based Tourism (CBT) in assessing the effectiveness of community-based ecotourism that also lack in a study conducted by Pookhao in 2014 entitled “Community-based Ecotourism: Transformation of the Local Community.” Quantitative descriptive research and purposive sampling was used with a sample size of 225 respondents through survey questionnaires that were personally distributed by the researchers. Percentage Frequency Distribution, T-Test and Analysis of Variance or ANOVA were used in analyzing the data. Based on the findings gathered, the respondents are mostly 26 to 35 years old, mostly males who are employed and have 10 years and above of residency. The objective of the study was to assess the effectiveness of community-based ecotourism in Brgy. Alas-As in San Nicolas, Batangas and to improve their quality of services, meet visitor s expectations and ensure the protection of the community and their natural and cultural heritage resources with an action plan.
Optimization of Tannase Production by Aspergillus niger in Solid-State Packed-Bed Bioreactor
( Rodriguez Duran Luis ),( Juan C. Contreras Esquivel ),( Raul Rodriguez ),( L. Arely Prado Barragan ),( Cristobal N. Aguilar ) 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.9
Tannin acyl hydrolase, also known as tannase, is an enzyme with important applications in the food, feed, pharmaceutical, and chemical industries. However, despite a growing interest in the catalytic properties of tannase, its practical use is very limited owing to high production costs. Several studies have already demonstrated the advantages of solid-state fermentation (SSF) for the production of fungal tannase, yet the optimal conditions for enzyme production strongly depend on the microbial strain utilized. Therefore, the aim of this study was to improve the tannase production by a locally isolated A. niger strain in an SSF system. The SSF was carried out in packed-bed bioreactors using polyurethane foam as an inert support impregnated with defined culture media. The process parameters influencing the enzyme production were identified using a Plackett-Burman design, where the substrate concentration, initial pH, and incubation temperature were determined as the most significant. These parameters were then further optimized using a Box-Behnken design. The maximum tannase production was obtained with a high tannic acid concentration (50 g/l), relatively low incubation temperature (30oC), and unique low initial pH (4.0). The statistical strategy aided in increasing the enzyme activity nearly 1.97-fold, from 4,030 to 7,955 U/l. Consequently, these findings can lead to the development of a fermentation system that is able to produce large amounts of tannase in economical, compact, and scalable reactors.
SHM by DOFS in civil engineering: a review
Rodriguez, Gerardo,Casas, Joan R.,Villalba, Sergi Techno-Press 2015 Structural monitoring and maintenance Vol.2 No.4
This paper provides an overview of the use of different Distributed Optical Fiber Sensor systems (DOFSs) to perform Structural Health Monitoring (SHM) in the specific case of civil engineering structures. Nowadays, there are several methods available for extracting distributed measurements from optical fiber, and their use have to be according with the aims of the SHM performance. The continuous-in-space data is the common advantage of the different DOFSs over other conventional health monitoring systems and, depending on the particular characteristics of each DOFS, a global and/or local health structural evaluation is possible with different accuracy. Firstly, the fundamentals of different DOFSs and their principal advantages and disadvantages are presented. Then, laboratory and field tests using different DOFSs systems to measure strain in structural elements and civil structures are presented and discussed. Finally, based on the current applications, conclusions and future trends of DOFSs in SHM in civil structures are proposed.
Real-time detection of acetylcholine release from the human endocrine pancreas
Rodriguez-Diaz, Rayner,Dando, Robin,Huang, Y Anthony,Berggren, Per-Olof,Roper, Stephen D,Caicedo, Alejandro Nature Publishing Group, a division of Macmillan P 2012 Nature protocols Vol.7 No.6
Neurons, sensory cells and endocrine cells secrete neurotransmitters and hormones to communicate with other cells and to coordinate organ and system function. Validation that a substance is used as an extracellular signaling molecule by a given cell requires a direct demonstration of its secretion. In this protocol we describe the use of biosensor cells to detect neurotransmitter release from endocrine cells in real-time. Chinese hamster ovary cells expressing the muscarinic acetylcholine (ACh) receptor M3 were used as ACh biosensors to record ACh release from human pancreatic islets. We show how ACh biosensors loaded with the Ca<SUP>2+</SUP> indicator Fura-2 and pressed against isolated human pancreatic islets allow the detection of ACh release. The biosensor approach is simple; the Ca<SUP>2+</SUP> signal generated in the biosensor cell reflects the presence (release) of a neurotransmitter. The technique is versatile because biosensor cells expressing a variety of receptors can be used in many applications. The protocol takes ?? h.
Rodriguez-Perez, Manuel,Villanueva-Cab, Julio,Pal, Umapada Techno-Press 2017 Advances in nano research Vol.5 No.3
Graphene oxide (GO) was prepared by modified Hummer's method to produce reduced graphene oxide (RGO) following standard thermal and chemical reduction processes. Prepared RGO colloids were utilized to fabricate RGO films over glass and FTO coated glass substrates through drop-coating. A systematic study was performed to evaluate the effect of reduction degree on the optical and electrical properties of the RGO film. We demonstrate that both the reduction process (thermal and chemical) produce RGO films of similar optical and electrical behaviors. However, the RGO films fabricated using chemically reduced GO colloid render better performance in dye sensitized solar cells (DSSCs), when they are used as counter electrodes (CEs). It has been demonstrated that RGO films of optimum thicknesses fabricated using RGO colloids prepared using lower concentration of hydrazine reducer have better catalytic performance in DSSCs due to a better catalytic interaction with redox couple. The better catalytic performance of the RGO films fabricated at optimal hydrazine concentration is associated to their higher available surface area and lower grain boundaries.