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Characterization of a gene encoding ornithine carbamoyltransferase from rice
Islam Sikdar, Shafiqul,Kim, Jung-Sup The Korean Society of Plant Biotechnology 2009 식물생명공학회지 Vol.36 No.4
Ornithinine carbamoyltransferase (OTC) is an enzyme that catalyzes the key step in arginine biosynthesis in bacteria and plants. OTC is also involved in the urea cycle and deficiency of the enzyme in human leads to disease. The argF gene encoding OTC has been reported in many bacteria and few plants. Here we report the characterization of a gene encoding OTC from rice (OsOTC). Analysis of a cDNA sequence from rice revealed that the full-length open reading frame of OsOTC consisted of 367 amino acids, corresponding to a protein of approximately 39.7 kDa. The predicted amino acid sequence of OsOTC harbor distinct five OTC signature sites and is highly homologous to that of enzymes of plants, animals and many bacterial OTCs. Expression of OsOTC in argF mutants of Escherichia coli showed that the gene was able to functionally complement to the mutant. These results suggest that the OsOTC encode a protein for ornithine carbamoyltransferase in rice.
Characterization of a gene encoding ornithine carbamoyltransferase from rice
Md. Shafiqul Islam Sikdar,김정섭 한국식물생명공학회 2009 식물생명공학회지 Vol.36 No.4
Ornithinine carbamoyltransferase (OTC) is an enzyme that catalyzes the key step in arginine biosynthesis in bacteria and plants. OTC is also involved in the urea cycle and deficiency of the enzyme in human leads to disease. The argF gene encoding OTC has been reported in many bacteria and few plants. Here we report the characterization of a gene encoding OTC from rice (OsOTC). Analysis of a cDNA sequence from rice revealed that the full-length open reading frame of OsOTC consisted of 367 amino acids, corresponding to a protein of approximately 39.7 kDa. The predicted amino acid sequence of OsOTC harbor distinct five OTC signature sites and is highly homologous to that of enzymes of plants, animals and many bacterial OTCs. Expression of OsOTC in argF mutants of Escherichia coli showed that the gene was able to functionally complement to the mutant. These results suggest that the OsOTC encode a protein for ornithine carbamoyltransferase in rice.
Sharmin Nahar,Md. Faruk Hasan,Sikdar Biswanath,Md. Asadul Islam 한국작물학회 2021 Journal of crop science and biotechnology Vol.24 No.5
The present investigation was carried out to investigate the efects of rhizosphere bacteria on growth and rice yields. Also, isolation and identifcation of rhizospheric bacterial treatments rhizosphere soil, mixed bulk, and fertilized soils. Soil analysis showed a maximum pH of 8.46 in rhizosphere soil of Sada dhepa. Nitrogen content of the rhizosphere soil of Latif sayil showed 0.18% maximum result. In-plant growth and yield, 87.37 cm leaf length, 28.83 cm panicle length, and 2.32 gm 100 seed weight in rhizosphere soil of Latif sayil were observed. Five diferent rhizobial bacteria Rhizobium tropici, Rhizobium leguminosarum, Rhizobium freirei, Rhizobium oryzae, Bacillus subtilis were isolated from the rhizosphere. Mixed treatment on Azospirillum largimobile, Azotobacter chroococcum, Rhizobium leguminosarum, Pseudomonas fuorescens were isolated from the rhizosphere. Mixed treatment was signifcantly similar to Rhizobium tropici and Azotobacter chroococcum by a maximum of 82.32% and 86.19%, respectively. Our results indicate that selected rhizobial strains promote rice growth and yields that could be harnessed to practical beneft for the farmer and are consistent with sustainable agricultural practices.