僧朗을 相承한 中國三論의 歷史性

金芿石 東國大學校 1964 論文集 Vol.1 No.-

해면질 장골이식을 이용한 치조열의 치료

류재만,김잉곤,엄기일,최봉근 大韓成形外科學會 1993 Archives of Plastic Surgery Vol.20 No.1

In a complete primary unilateral cleft of the primary and secondary palates, there is frequently a significant bony defect in the maxilla. The most significant skeletal changes in patients with clefts are maxillary retrusion and collapse of the maxillary segment. The alveolar cleft in the disrupted dental arch frequently accentuates facial disfigurement because the anterior maxillary teeth may be malpositioned, malformed, or congenitally absent. Defects or hypoplastic pyriform aperture is a major external factor which causes the unilateral cleft lip nise deformity. It also causes deformities of alar cartilage and nasal tip and septal deviation, which has been well explained with the concept of 'Tilted Tropod' by hogan and Converse in 1971. The reason for closing alveolar clefts with a bone graft is to provide the maxillary arch stability and to create bony matrix through which the teeth can erupt and to construct the plateform of the alar base for improving nasal symmetry. For the closing of the alveolar cleft, an autogenous rib bone, a tibial bone or recently calvarial bone have been used, but serious donor site morbidity and poor source of recently calvarial bone have been used, but serious donor site morbidity and poor source of cancellous bone turned out to be problems. The authors used a cancellous iliac bone graft on 20 patients for the recovery of the alveolar cleft. Of these, 18 patients were in permanent dentition and 2 patients were in mixed dentition. Approximately 95 percent of the authors' patients have undergone successful closure of the alveolar defect and noticed teeth eruption through the grafted bone in all patients who grafted in mixed dentition. We performed external rhinoplasty upon the symmetric aperture corrected with cancellous iliac bone graft and achieved satisfactory aesthetic results, thus we represent our methods and cases with review of the literature.

음부를 포함한 다발성 다유방증의 치험례

이신규,김잉곤,최회윤,류재만,조삼현 대한성형외과학회 1992 Archives of Plastic Surgery Vol.19 No.3

Accessory breast tissue is developed from persistence of breast tissue along the milk line which first beomes apparent at the 6th week of embryo and usually atrophies except for the thoracic area until the 9th week. It is much more common than amastia and occurs in approximately from 2 to 6 percent of the population. But multiple polymastia involving labia major is quite rare. We experienced one case of multiple polymastia involving labia majora & both axilla and 6 cases of polymastia & polyhelia, so we are willing to report our cases with a review of literature.

Bleomycin이 모낭의 기질세포에 미치는 영향에 관한 전자현미경적 연구

이영만,김잉곤,류재만 한양대학교 의과대학 1993 한양의대 학술지 Vol.13 No.2

Bleomycin, one of antibiotic drug, produced by a strain of Streptomyces verticillus is used effectively in the treatment of various cancer. It is well known that bleomycin acts on the cancer cell by binding to nuclear DNA, causing single-strand scission and in turn inhibition DNA synthesis. Although bleomycin is very beneficial to cancer patient, it may damage ot normal cells. The author has undertaken this study to pursue the morphological changes of hair folliclis caused by bleomycin injection. Healthy male mice of DDY strain weighing about 20 gm were used in this experiment. Animals of the experimental group were intraperitoneally administrated with 15mg/kg bleomycin diluted in 0.2ml distilled water for injection by bid for 7days. The experimental animals were sacrificed at 1,3days, and 1,2 weeks after administration of bleomycin and specimens, skin of abdomen, were rejected. Some specimens were fixed 10% formalin for 24 hours and stained with hematoxylin and eosin. The others were prefixed in 2% glutaraldehyde-2.5% paraformaldehyde solution at 4℃ during 2-4 hours and postfixed in 1% osmium tetroxide solution. Ultrathin sections were made and double stained with urany1 acetate and lead citrate. These preparations were observed with light microscope and electron microscope (Hitachi H-600), respectively. The results were as follows: 1.The number and length of the hair folliclis were decreased and number of matrix cells were also reduced in skin in 1 day, 3 day and 1 week groups after administration of bleomycin. In 2 week group, hair follicles were similar to those of the normal control group. 2.Swollen mitochondria, mitochondria with indistinct double membranes and mitochondrial cristae, and degenerated mitochondria with horse-shoe shape were recognized cristae, and degenerated mitochondria with horse-shoe shape were recognized in 1 day, 3 day and 1 weed groups after bleomycin administration. In 2week group, swollen mitocondria and mitochondria with disorganized mitochondrial cristae and double membranes were observed. 3.The amounts of free ribosomes were markedly reduced in 1 day group after administration of bleomycin and slightly decreased in 3 day and 1 week groups. In 2 week group, distribution of free fibosomes were similar to that of the normal control gorup. 4.The cisternae of the rough endoplasmic reticulum with detachment of membrane-bound ribosomes were slihtly dilated in 1 day, 3 day groups after administration of bleomycin. In 2 week group, rough endoplasmic reticulum were similar to that of the normal control group. 5.A number of autophagic vacuoles were observed in 1 day, 3 day groups after administration of bleomycin and whorled membranous body was seen in 1 week group. Consequently, it is suggested that bleomycin would induce degenerative changes on the matrix cells of the hair follicle in mouse skin, but these damage are recovered as time goes by.

복벽 성형술의 문제점 및 분류

최희윤,류재만,김잉곤,차상면,엄기일 大韓成形外科學會 1991 Archives of Plastic Surgery Vol.18 No.2

Some patients requesting abdominal contour surgery may have all their deformity below the semicircular line. These patients were treated by the traditional procedure, but these patients has nerve satisfied because of long and wide scar. From a study of the deformities of each layer of the abdominal wall, we have categorized three types of abdominoplasties. For each type, we used a different surgical technique, aiming to sculpture the abdomen and treat each layer according to the deformity present in each patient. By classifying the various problems and by using the different approaches, only the specific deformities of the abdominal wall need be corrected. The results were judged good excellent by the surgeons and patients because of the more natural appearance of the final results.

Ortho-Chlorobenzylidene Malononitrile(CS) 이 흰쥐 피부조직에 미치는 조직학적 영향에 관한 연구

이우용,최봉근,김잉곤,엄기일,정호삼,정호삼,류재만 한양대학교 의과대학 1998 한양의대 학술지 Vol.18 No.1

This study was undertaken to describe the fine structural changes of cytoplasmic organelles in epidermal cells, and the changes of volume and localization of collagen type Ⅰ in the dermis of rat skin by CS(Ortho-chlorobenzylidene malononitrile) application. The experimental animals, 100∼150gm of body weight rats(Sprague-Dawley strain), were sacrificed at 12 hours, 24 hours, 3 day and 5 day after CS application on the hair-shaved back skin. The specimens were prepared for ultrastructural findings, ultrathin sections were stained with uranyl acetate and lead citrate double stain and observed with Hitachi-600 electron microscrope. The others were incubated with rabbit anti rat type Ⅰ collagen antibody as primary antibody, and Goat anti rabbit IgG antibodies as secondary antibody And advidin-biotin-conjugated(ABC) peroxidase procedure was used. All of specimens for immune reactions were observed with light microscope. The results obtained were as follows. 1 Keratinocytes appeared fully necrotized after 3 days of CS application. The epidermis showed degeneration of basal lamina, enlargement of intercellular spaces and necrotic keratinocytes with vacuole and vesicles. And large keratohyalin granules were seen in the shrunken epidermal cells. 2 At 3 or 5 day after CS treatment, the normal cytoplasmic organelles of keratinocytes began to appear on the basal and prickel cells. 3 At 12 hour an 24 hour after CS treatment. The immune reaction of type Ⅰ collagen showed weak reaction in reticular layer of dermis. But at 3 day and 5 day after CS treatment, moderate immune reaction were seen in throughout the dermis. These results suggest that CS application to rak skin may induce changes of keratinocytes and reductive changes of type Ⅰ collagen in the dermis.

절제된 백서의 좌골 신경에서 절제 원위부의 변화에 따른 신경재생의 비교 실험연구

김정태,엄기일,김잉곤,류재만,이혜성 大韓成形外科學會 1991 Archives of Plastic Surgery Vol.18 No.2

성숙 백서 좌골신경의 분지전 근위부에서 7㎜가량을 절제하여 신경 간격을 만든뒤 실라스틱도관 근위단에 절단된 신경의 근위부를 관통고정하고 원위부에는 아무것도 열결하지 않거나 좌골신경 절편, 종골건 절편, 안면신경 절편을 각각 관통고정시켜 제4주 및 8주에 신경 축색수를 대조군과 혹은 각 실험군끼리 비교하여 다음과 같은 결론을 얻었다. 1. 신경 절편을 연결헌 2,4군의 신경재생은 대조군과 거의 차이가 없었고(P>0.05) 아무것도 연결하지 않은 1군, 건절편을 연결한 3군에 비해 현격한 신경재생을 보였다.(P<0.01). 2. 신경 절편의공여부에 관계없이 2,4군의 실험군에서 각각 비슷한 신경재생을 보여 일단 신경이 손상되면 신경 성장인자가 손상된 신경단으로 부터 분비되며 종류에 관계없이 그 근위부의 신경 재생을 자극할 수 있음을 알 수 있었다. 3. 건절편을 연결한 3군의 실험군에서는 일부 신경재생이 일어나는 것으로 보아 여러가지 가능성을 제시할 수 있었다. 이상에서 같이 신경 재생에 대한 기전은 아직까지 밝혀지지 않고 많은 논란의 대상이 되고 있는 것이 사실이나 저자들은 이에 조금이라도 접근해보고자 본 실험을 시도하였고 많은 의문점이 제시되었으나 만족할 만한 결과를 얻었다. 앞으로 보다 더 세밀한 비교관찰과 많은 개체수를 이용한 실험이 필요하겠고 축색수 이외에도 수초의 두께, 수초와 축색의 비율, 유수 및 무수신경수의 비율등도 함께 비교하는 정확한 결과의 분석이 필요하리라 생각된다. An experimental model was presented for studying nerve regeneration over constant gaps of various distal stumps such as, empty tube(GroupⅠ), sciatic nerve segment(GroupⅡ), calcaneal tendon segment(Group Ⅲ), facial nerve segment(Group Ⅳ), to ascertain how the regenerating fibers of the proximal stump of the severed sciatic nerves of the rats are oriented and processed. In this experiment, the silastic tube was used as a guide to observe the sprouting of the regenerating fibers from proximal stump and then the regenerating fibers in the tubes were obtained and analyzed by light microcope, electron microscope and axon counts at 4 weeks and 8 weeks with the evoked action potential at 8 weeks. The results were as follows. 1. The axon counts of experimental group Ⅱ and Ⅳ with nerve segment stump were similar to those of their control groups(P>0.05) and were greater than those of experimental groupⅠ and Ⅲ(P<0.01). 2. The axon counts between the experimental group Ⅱ and Ⅳ were nearly indistinguishable and so nerve segments, irrespective of donor site in the same rat, induced the regenerating stimulus to their proximal stump of resected sciatic nerve. 3. In experimental group Ⅲ, the obtaining results of axon counts were not negligible, in spite of tendon stump, and the author could propose various possibilities. In conclusion, we could approach to the regenerating process of the nerve through the analysis of the above results but yet can not explain the exact mechanism. Anyway when the results are applied to the clinical situation, we would be helped much.

흰쥐태아 구개돌기의 융합에 관여하는 E-cadherin과 세포자사에 관한 연구

엄기일,차지훈,김우신,김잉곤,정호삼 大韓成形外科學會 2001 Archives of Plastic Surgery Vol.28 No.5

Yarrowia lipolytica TH65가 생산하는 Alkaline Proteinase의 정제 및 특성

유춘발,김창화,진영호,진익렬 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.3

최적조건에서 생산한 효소를 40~65% ammonium sulfate 분획, DEAE-cellulose chromatography, Sephadex G-100과 Sephadex G-75 gel filtration을 이용하여 수율 14%, 15배 정제하였다. 정제효소의 분자량은 SDS-PAGE로 측정한 결과 31,500 정도였고, 최적온도와 최적 pH는 각각 40℃와 8.5~9.0이었다. 대부분의 2가 금속이온은 효소활성을 저해하였으며, 특히 Hg^2+, Zn^2+, Co^2+ 등은 강하게 저해하였다. PMSF는 효소활성을 완전히 저해시켰으며, EDTA, EGTA 및 phenanthroline은 부분적으로 저해시켰으나 Ca^2+을 첨가하면 모두 효소활성이 복원되는 것으로 나타나 metalloprotease는 아닌 것으로 생각되었다. 이상의 결과로 보아 본 효모 Yarrowia lipolytica TH65가 생산하는 효소는 alkaline serine proteinase로 생각된다. An alkaline proteinase produced by Yarrowia lipolytica TH65 was purified by 40~65% ammonium sulfate fractionation, DEAE-cellulose chromatography, and gel filtration with Sephadex G-100 and Sephadex G-75. The purified enzyme was shown as a single ban on SDS-PAGE, and its molecular weight 31,500. Optimum temperature and pH were 40℃ and 8.5~9.0, respectively, and the enzyme was stable below 40℃ and in the pH range of 6~8. The enzyme was strongly inhibited by divalent ions, completely by PMSF, and partially by EDTA, EGTA, and phenanthroline. But the inhibitory effect in the presence of EDTA, EGTA and phenanthroline could be reversed by addition of Ca^2+. Thus, these results indicated that the purified enzyme was an alkaline serine proteinase (E.C. 3.4.21.14).

Microform Cleft Lip 환자의 분류에 따른 수술적 치료

박철수,엄기일,황세휘,안덕균,김잉곤 大韓成形外科學會 1999 Archives of Plastic Surgery Vol.26 No.3

The microform cleft lip is the mildest expression of cleft lip and nose deformity, but it has no specific definition, classification, and few methods have been reported for its correction. It is characterized by deformity of the nostril, skin striae of the upper lip, notching of peaked Cupid's bow, deformity of the vermilion, and anomaly of the upper lateral incisior and alveolar ridge on the affected region. Sixty-three microform cleft lip patients were operated on between Dec. 1993 and Sep. 1998 in our department(29 males and 34 females). The age of the patients ranged from 5 months to 30 years(Mean 9 years). We classified and treated the microform cleft lip as follows: Class Ⅰ: Cleft lip nose with very slight lip deformity Class Ⅱ: Minimal lip deformity without vermilion notching ClassⅢ: Mild lip deformity with slight vermilion notching. The goals in the correction of a microform cleft lip are to obtain an esthetically pleasing upper lip and nose, and to reestablish muscle continuity for improved function. To attain these goals, we used the above classification and satisfactory results were obtained by treating the microform cleft according to the classification.