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Indrakant Kumar Singh,Sumit Kumar,Sujata Singh,Archana Singh 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.3
The Mitogen-activated Protein Kinase (MAPK) Cascades play a crucial role in the regulation of defense responses in plants. Herbivory- or wounding-related MAPKs, SA-induced protein kinase (SIPK) and wound-induced protein kinase (WIPK) have been identified to be associated with insect attack. However, function of MAPKs in crop plants in response to herbivory largely remains unknown. Here, we evaluated the expression patterns of fifteen MAPK genes from chickpea in response to infestation by a major pest, Helicoverpa armigera via quantitative realtime PCR (qPCR). Subsequently, we compared the expression patterns of CaMAPKs in response to two related but different processes i.e. insect attack and mechanical wounding. Signaling compounds such as Jasmonates, Salicylic acid and Ethylene also play crucial function in defense response. Therefore, we have also examined the expression profiles of H. armigera-inducible MAPK genes in response to these signaling compounds. Our results revealed that out of fifteen CaMAPKs, seven of the CaMAPKs are upregulated in response to H. armigera and two are downregulated. Additionally, they show differential expression on comparing Helicoverpa-infestation and wounding. Jasmonates and Ethylene mainly regulate CaMAPK genes that may play a role in defense against Helicoverpa-infestation. This study provides insights into the participation of MAPKs in defense against insects.