rnpB 유전자 발현에 RNA 이차구조가 미치는 영향

전은순,이영훈,박충웅,조인호 全北大學校 基礎科學硏究所 1988 基礎科學 Vol.11 No.1

E. coli rnpB gene의 발현에 영향을 미치는 M1 RNA structural gene의 부분을 조사하였다. P-1 promoter 에서 합성되는 RNA의 5' 부분이 이루는 이차구조를 분석한 결과 +1 에서 부터 +72 까지에 존재하는 hairpin loop 구조가 rnpB gene의 발현에 중요한 역할을 하는 것으로 밝혀졌다.

상부 소화관 이물 78예에 대한 임상적 고찰

이승훈,서인석,김은화,최용우,이기세,이용웅 대한소화기학회 1998 대한소화기학회지 Vol.31 No.5

Background/Aims: Impaction of a foreign body in the upper gastrointestinal (UGI) tract is common in children and old ages, It is recommended to remove the foreign bodies if they produce symptoms, are retained in UQI tract for long period, or have the possibilities of producing complications. The purpose of this study is to characterize the climical feature of fareign bodies in UGI tract. Methods: By means of flexible endoscopy, we have examined 87 patients with suspicion of UGI foreign body among the patients visiting our medical center from January 1992 to June 1997. The 78 patients of them were proven to have foreign bodies in UGI tract. Results: They are prevailing in patients over 60s. For the remaining 9 patients, despite having a history of foreign body ingestion and symptoms, subsequent investigation failed to demonstrate the impaction of foreign bodies. The most comman fareign bodies were fish bones and the most common symptom was throat discomfort. The most frequent localization of the impaction was the cricopharyngeal sphincter. In 97% of the cases, the fareign bodies could be removed endoscopically with a few complications. Conclusions: Clmical history is the main indicator in the decision to perform endoscopic removal of suspected foreign bodies. We suggest that endoscopic removal as soon as possible is recommenended even if the foreign body is only suspected.

Enhanced fungal resistance in Arabidopsis expressing wild rice PR-3 (OgChitIVa) encoding chitinase class IV

Jung-Hun Pak,Eun-Sook Chung,Sang-Hyun Shin,Eun-Hee Jeon,Mi-Jin Kim,Hye-Young Lee,Ji-Ung Jeung,Nam-In Hyung,이재헌,Young-Soo Chung 한국식물생명공학회 2009 Plant biotechnology reports Vol.3 No.2

Oryza grandiglumis Chitinase IVa (OgChitIVa) cDNA encoding a class IV chitinase was cloned from wild rice (Oryza grandiglumis). OgChitIVa cDNA contains an open reading frame of 867 nucleotides encoding 288 amino acid residues with a predicted molecular weight of 30.4 kDa and isoelectric point of 8.48. Deduced amino acid sequences of OgChitIVa include the signal peptide and chitin-binding domain in the N-terminal domain and conserved catalytic domain. OgChitIVa showed significant similarity at the amino acid level with related monocotyledonous rice and maize chitinase, but low similarity with dicotyledoneous chitinase. Southern blot analysis showed that OgChitIVa genes are present as two copies in the wild rice genome. It was shown that RNA expression of Og- ChitIVa was induced by defense/stress signaling chemicals, such as jasmonic acid, salicylic acid, and ethephon or cantharidin and endothall or wounding, and yeast extract. It was demonstrated that overexpression of OgChitIVa in Arabidopsis resulted in mild resistance against the fungal pathogen, Botrytis cinerea, by lowering disease rate and necrosis size. RT-PCR analysis showed that PR-1 and PR-2 RNA expression was induced in the transgenic lines. Here, we suggest that a novel OgChitIVa gene may play a role in signal transduction process in defense response against B. cinerea in plants.

Studies on the Effect of Silicon Nutrition on Plant Growth, Mineral Contents and Endogenous Bioactive Gibberellins of Three Rice Cultivars

Jang, Soo-Won,Hamayun, Muhammad,Sohn, Eun-Young,Shin, Dong-Hyun,Kim, Kil-Ung,Lee, In-Jung The Korean Society of Crop Science 2007 Journal of crop science and biotechnology Vol.10 No.1

Silicon is one of the key elements for healthy growth and development in rice crops. We analyzed the effect of silicon(Si) on some growth parameters, plant mineral contents, and bioactive gibberellins in three rice cultivars. Silicon was applied at the rates of 0 kg/0.1ha(control), 40 kg/0.1ha, and 80 kg/0.1ha throughout the course of experiment. Plant growth parameters were enhanced by the application of elevated Si, though plant height and culm length were more favorably affected than the respective dry weights. The plant mineral contents analyzed also increased in treatments where Si was applied without potassium, demonstrating that Si application promotes the absorption of these minerals in rice crops. The endogenous gibberellins measured in our study showed that $GA_1$ is the only bioactive GA form present in rice seedlings. The endogenous $GA_1$ and its precursor $GA_{20}$ contents increased after Si application. However, this increase in endogenous $GA_1$ and $GA_{20}$ contents, and plant growth parameters were different according to the rice cultivars. Our results indicate that Si is a beneficial element in rice nutrition and that different cultivars of Oryza sativa show differential responses to Si nutrition in terms of their growth and development.

Evaluation of Pollen Viability of Nakdongbyeo, Two Transgenic Rice Lines, ItsHybrids with Weedy Rice, and Subsequent Selfed Progenies : F₂ and F₃

Sita Ram Ghimire(기미어 시다람),Eun-Young Sohn(손은영),Dong-Hyun Shin(신동현),In-Jung Lee(이인중),Kil-Ung Kim(김길웅) 한국생명과학회 2009 생명과학회지 Vol.19 No.7

본 연구는 비유전자변형 계통 (낙동벼)과 2개의 다른 promoter (maize ubiquitin과abscisic acid)를 각각 삽입한 유전자변형 벼와 abscisic acid promoter이용한 유전자변형 계통을 잡초성벼 (Oryza sativa)와 인공수정 한 후 다음세대인 F₁, F₂, F₃의 화분활력, 형태형성, 생장차이를 비교하여 평가하였다. 화분이 열개된 후 3-{4,5dimethylthiazolyl-2}-2,5-diphenyl monotetrazolium bromide (MTT)반응을 살펴본 결과, Nakdongbeyo에서 86%, ABC-promoter 이용한 유전자변형 벼에서 75%, maize ubiquitin promoter 이용한 유전자변형 벼에서 62%, F1에서 68%, F₂에서 79% 및 F3에서 78%의 각각 최대 화분활력을 보여주었다. 유전자변형 계통과 잡초성벼와의 교잡종의F₁, F₂, F₃ 세대간의 화분활력을 비교하였을 때 화분 열개된 후 20분까지는 유의한 차이를 보이지 않았으나, 40분에서 90분 사이에서는 F₃의 화분활력이 다른 두 세대 F₁, F₂보다 높게 나타났다. 화분을 열개된 90분 후 F₃ 세대 에서 최대 화분 활력이 36.2% 이었고 F2 세대 에서는 화분 활력이 최소 3.5% 보였다. 따라서 화분에 의해 벼의 유전자가 다른 계통으로 전이되는가를 조사하기 위하여 연차실험을 수행하였는데, 결론적으로 두 유전자변형 계통으로부터 잡초성벼로 유전자가 전이될 위험성이 나타날 것으로 보였다. This experiment was conducted to evaluate pollen viability of Nakdongbyeo, transgenic rice lines, an F1 hybrid from a cross between Milyang weedy rice and ABC-promoter transgenic rice line containing basta-resistant (bar) gene and subsequent selfed progenies, F₂ and F₃. The reaction of pollen with 3-{4,5 dimethylthiazolyl-2}-2,5-diphenyl monotetrazolium bromide (MTT) as a staining chemical immediately after pollen shedding showed maximum pollen viability of 86% in Nakdongbeyo, 75% in ABC-promoter transgenic rice line, 62% in ubiquitin-promoter transgenic line, 68% in F1, 79% in F₂ and 78% in F₃. Viability gradually declined during subsequent observations at 20-minute intervals. However, there was a drastic decline in pollen viability after 40 minutes of pollen shedding. The mean difference of pollen viability among rice lines and time was highly significant, indicating significantly different pollen viabilities at different time intervals. Maximum viability of 36.2% was observed in F₃ and minimum viability of 3.5% was found in F₂ at 90 min after pollen shedding. Results of this experiment on pollen viability and longevity elucidate potential risks of pollen-mediated flow of herbicide-resistant gene from transgenic rice lines and possible integration of it into the weedy rice population.

노년기 정신장애의 전기경련치료

주은정,김희철,강웅구,이남영,박승현,김정민,김용식,정인원,Joo, Eun-Jeong,Kim, Hee Cheol,Kang, Ung Gu,Lee, Nam Young,Park, Seung Hyun,Kim, Jung Min,Kim, Yong Sik,Chung, In Won 대한생물정신의학회 2020 생물정신의학 Vol.27 No.2

Electroconvulsive therapy (ECT) is indicated for various mental disorders (e.g., major depressive disorder, schizophrenia, and bipolar disorder) and the behavioral and psychological symptoms of dementia in elderly patients. Furthermore, ECT is a useful first-line treatment in emergency and crisis situations such as suicide risk, violent behavior, catatonia, and food refusal, which are more frequent in elderly patients. ECT is also effective in the treatment of the motor symptoms of neurological disorders, such as Parkinson's disease and Huntington's disease. Due to the high risk of various physical diseases, the comorbid physical conditions of elderly patients should be individually controlled to optimize ECT treatment. Compared to young adults, in elderly patients the seizure threshold is higher, the seizure duration is shorter, and the anesthetic dose is lower. On the contrary, the response rate in the elderly is both faster and higher. Considering potential cognitive decline and the prevention of further deterioration of cognitive function in elderly patients, in the absence of significant comorbidities, twice weekly sessions and right unilateral electrode placement with a lower seizure threshold and less cognitive effect are preferred to bilateral electrode placement, which has a high risk of adverse cognitive effects. After an acute course of ECT, continuation and maintenance of ECT, combined with prescription of therapeutic drugs, may prevent possible relapse or recurrence of mental disorders. In conclusion, ECT can be used to treat mental disorders in elderly adults, with safety and effectiveness comparable to that in young adults.

Effects of Insulation Layer upon the Thermal Behavior of Linear Motors

In-Ung Eun 대한기계학회 2003 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.17 No.6

A linear motor has many advantages next to conventional feed mechanisms: high transitional speed and acceleration. high control performance. and good positioning accuracy at high speed. Through the omission of a power transfer element. the linear motor shows no wear and no backlash. has a long lifetime. and is easy to assemble. A disadvantage of the linear motor is low efficiency and resultant high-temperature rise in itself and neighboring structUres during operation. This paper presents the thermal behavior of the linear motor as a feed mechanism in machine tools. To improve the thermal behavior. an insulation layer is used. By placing the insulation layer between the primary part and the machine table. both the temperature difference and the temperature fluctuation in the machine table due to a varying motor load are reduced.

Comparison between Asynchronous and Synchronous Linear Motors as to Thermal Behavior

In-Ung Eun 한국정밀공학회 2001 International Journal of Precision Engineering and Vol.2 No.3

OPTIMIZATION OF THE PARAMETERS OF FEEDWATER CONTROL SYSTEM FOR OPR1000 NUCLEAR POWER PLANTS

UNG SOO KIM,IN HO SONG,JONG JOO SOHN,EUN KEE KIM 한국원자력학회 2010 Nuclear Engineering and Technology Vol.42 No.4

In this study, the parameters of the feedwater control system (FWCS) of the OPR1000 type nuclear power plant (NPP)are optimized by response surface methodology (RSM) in order to acquire better level control performance from the FWCS. The objective of the optimization is to minimize the steam generator (SG) water level deviation from the reference level during transients. The objective functions for this optimization are relationships between the SG level deviation and the parameters of the FWCS. However, in this case of FWCS parameter optimization, the objective functions are not available in the form of analytic equations and the responses (the SG level at plant transients) to inputs (FWCS parameters) can be evaluated by computer simulations only. Classical optimization methods cannot be used because the objective function value cannot be calculated directely. Therefore, the simulation optimization methodology is used and the RSM is adopted as the simulation optimization algorithm. Objective functions are evaluated with several typical transients in NPPs using a system simulation computer code that has been utilized for the system performance analysis of actual NPPs. The results show that the optimized parameters have better SG level control performance. The degree of the SG level deviation from the reference level during transients is minimized and consequently the control performance of the FWCS is remarkably improved.

Effects of Insulation Layer upon the Thermal Behavior of Linear Motors

Eun, In-Ung The Korean Society of Mechanical Engineers 2003 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.17 No.6

A linear motor has many advantages next to conventional feed mechanisms: high transitional speed and acceleration, high control performance, and good positioning accuracy at high speed. Through the omission of a power transfer element, the linear motor shows no wear and no backlash, has a long lifetime, and is easy to assemble. A disadvantage of the linear motor is low efficiency and resultant high-temperature rise in itself and neighboring structures during operation. This paper presents the thermal behavior of the linear motor as a feed mechanism in machine tools. To improve the thermal behavior, an insulation layer is used. By placing the insulation layer between the primary part and the machine table, both the temperature difference and the temperature fluctuation in the machine table due to a varying motor load are reduced.