가교제 및 pH 조절제가 수용성 아크릴 점착제의 점착 물성에 미치는 효과

서인선,엄홍철,이명천 동국대학교 산업기술연구원 2001 산업기술논문집 Vol.12 No.1

본 연구에서는 점착제의 유지력 개선과 pH 조절을 위해 각각 첨가해주는 가교제와 pH 조절제의 종류 및 첨가량이 수용성 아크릴 점착제의 점착 물성 및 수용성에 미치는 효과를 알아보기 위하여 중합된 점착 시료에 여러 가지 가교제와 pH 조절을 첨가해 준 후 초기점착력, 유지력 및 점착력 등의 점착 물성과 수용성을 측정하였다. 가교제를 0.2 wt% 미만 첨가해주었을 때, 초기 점착력은 감소하였고 유지력과 점착력은 증가하였다. 그러나 0.2 wt% 이상 첨가해주었을 때는 초기 점착력은 증가하였지만 유지력과 점착력은 감소하는 결과를 보였다. 또한 pH 조절제를 첨가해주었을 때 pH 조절제의 농도가 증가함에 따라 초기점착력과 수용성은 감소하였고 점착력과 유지력은 증가하였으며, LiOH, KOH, NaOH 순으로 점착 물성과 수용성에 미치는 영향이 큰 것으로 나타났다. In this paper, the effects of crosslinking agent and pH controlling agent on adhesive properties and water solubility of water soluble acrylic pressure sensitive adhesives were studied by adding the crosslinking agents of pH controlling agents after the polymerization. The tack, cohesive strength, and peel strength were measured to evaluate adhesive properties. Turbidity was also measured to evaluate water solubility of pressure sensitive adhesives. When the crosslinking agent was added less than 0.2 wt%, the tack increased and cohesive strength and peel strength decreased with increasing concentration. When the crosslinking agent was added more than 0.2 wt%, the results were opposite. When the pH controlling agent was added, the tack and water solubility decreased and peel strength and cohesive strength increased with increasing concentration. The influence of pH controlling agents of adhesive properties and water solubility of pressure sensitive adhesives increased in the order of lithium hydroxide, potassium hydroxide and sodium hydroxide.

Isolation and Characterization of Micrococcaceae in Dental Clinic

Kim, Kang-Ju,Yoo, Soo-Kyung,Kim, Hyeon-Jin,Kim, Seon-Ju,Um, In-Woong The Official Publication of Korean Academy of Oral 1993 International Journal of Oral Biology Vol.17 No.1

It has been reported that staphylococci present in clinic and personnel are transmissible to patients. The purpose of this study is to ascertain the distribution. the drug resistance and plasmid pattern in Micrococcaceae isolated in dental clinic. A dental clinic was monitored in the morning in the afternoon and in the evening. S. hemolyticus was isolated from the cover of instruments and S. hominis was isolated from the margin of tray in the morning. S. epidermidis was isolated from light switch and head rest. S. aureus from cuspidor and head rest. and coagulase-negative staphylococci from suction tip in the afternoon. S. aureus was isolated from head rest and coagulase-negative staphylococci was isolated from suction rubber tip in the evening. Micrococci were isolated from the forceps tip and the switch in the evening. Staphylococci isolated in the afternoon showed susceptibility to ceftriaxone. and those isolated in the evening to ampicillin. carbenicillin. tetracyclin. gentamycin. amikacin. cephalothin. ceftriaxone and cefoxitin. Micrococcus isolated in the evening showed susceptibility to 15 antibiotics. In the analysis of plasmid. there were two clear bands about 4.3 kb, in S. aureus isolated from the head rest in the afternoon. These results show that Micrococcaceae can become ambient in dental clinic. and staphylococci isolated then show antibiotic resistance to some drugs.

Quantitative of Biogenic Amines in Commercial Salted and Fermented Sand Lance, Ammodytes personatus, Sauce

In-Seon Um,Tae-Ok Kim,Mi-Ran Park,Tae-Hwan Hong,Na-Young Lee,Yong-Tae-Kim,Jae-Geun Koo,Kwon-Sam Park 한국수산해양기술학회(구 한국어업기술학회) 2015 한국수산해양기술학회 학술발표대회 Vol.2015 No.10

Quantitative of Biogenic Amines in Commercial Salted and Fermented Sand Lance, Ammodytes personatus, Sauce

In-Seon Um,Tae-Ok Kim,Mi-Ran Park,Tae-Hwan Hong,Na-Young Lee,Yong-Tae-Kim,Jae-Geun Koo,Kwon-Sam Park 한국수산과학회 양식분과 2015 한국수산과학회 양식분과 학술대회 Vol.2015 No.10

시판 까나리(Ammodytes personatus) 액젓의 biogenic amines 함량

엄인선 ( In Seon Um ),박권삼 ( Kwon Sam Park ) 한국수산과학회 2015 한국수산과학회지 Vol.48 No.6

This study investigated the quality of seven commercial salted and fermented sand lance Ammodytes personatus sauces by measuring their chemical compositions, bacteria concentrations, and biogenic amine contents. The sauces had a 63.97-67.32% (mean: 65.72%) moisture content, 23.16-24.03% (mean: 23.60%) salinity, 5.51-6.14 (mean: 5.81) pH, 164.76-217.98 mg/100 g (mean: 182.97 mg/100 g) volatile basic nitrogen, 0.94-1.42% (mean: 1.24%) total nitrogen, and 673.77-791.86 mg/100 g (mean: 720.76 mg/100 g) amino nitrogen content. Viable cell counts ranged from 1.2×101 to 2.9×102 CFU/mL, and number of biogenic amine-forming bacteria was very low or not detected. Overall, the tested samples had average levels of histamine of 301.02 mg/kg, cadaverine of 29.78 mg/kg, tyramine of 199.21 mg/kg, putrescine of 183.00 mg/kg, and tyramine of 13.01 mg/kg. This strongly suggests that it is necessary to monitor the biogenic amine contents of commercial salted and fermented sand lance sauces carefully to ensure consumer health.

시판 까나리(Ammodytes personatus) 액젓에서 putrescine 생성균의 분리 및 특성

엄인선 ( In-seon Um ),김태옥 ( Tae-ok Kim ),박권삼 ( Kwon-sam Park ) 한국수산과학회(구 한국수산학회) 2016 한국수산과학회지 Vol.49 No.5

Bacterial decarboxylation of amino acids in food leads to the production of biogenic amines, which can cause reactions in human that include headaches, nausea, palpitations, chills, and severe respiratory distress. The amine putrescine is an especially effective inhibitor of metabolizing enzymes and amplifies histamine intoxication and tyramine poisoning. Using an L-ornithine decarboxylating medium, we isolated 14 putrescine-producing bacteria from sand lance, Ammodytes personatus, sauces. The isolates were identified, using an API kit and 16S rRNA analysis, as Lysinibacillus fusiformis (1 strain), Lysinibacillus xylanilyticus (6 strains), Lysinibacillus macroides (1 strain), Lysinibacillus sphaericus (3 strains), Bacillus fusiformis (1 strain), Paenibacillus favisporus (1 strain), and Staphylococcus caprae (1 strain). These strains produced between 1.66 to 236.97 μg/mL of putrescine after 48 h incubation. Lysinibacillus spp. were the dominant putrescine-producing bacteria in sand lance sauces, which produced 236.97 μg/mL of putrescine from a culture broth containing 0.5% L-ornithine. This is the first report on the isolation and identification of putrescine-producing bacteria from sand lance sauces.

시판 까나리(Ammodytes personatus) 액젓에서 분리한 tryptamine 생성균의 특성

엄인선 ( In-seon Um ),김태옥 ( Tae-ok Kim ),김희대 ( Hee-dai Kim ),박권삼 ( Kwon-sam Park ) 한국수산과학회(구 한국수산학회) 2016 한국수산과학회지 Vol.49 No.6

We isolated seven tryptamine-producing bacteria from commercial salted and fermented sand lance (Ammodytes personatus) sauces using an L-tryptophan decarboxylating medium. These tryptamine-producing bacteria, identified using an API kit and 16S rRNA analysis, included Lysinibacillus xylanilyticus (one strain), Lysinibacillus fusiformis (four strains), and Staphylococcus epidermidis (two strains). Lysinibacillus spp. produced the highest levels of tryptamine in culture broth containing 0.5% L-tryptophan, compared with 1.0% and 2.0% preparations. After 72 h of incubation, Staphylococcus epidermidis produced the highest levels of tryptamine (60.50 μg/mL and 664.86 μg/ mL) in culture broth containing 2.0% L-tryptophan. While Lysinibacillus spp. comprised the dominant tryptamine-producing bacteria in sand lance sauces, Staphylococcus epidermidis also showed high tryptamine-producing active-ity. This is the first report on the isolation and identification of tryptamine-producing bacteria in sand lance sauces.

Comparison of biomechanical properties between acupuncture and non-penetrating sham needle

Chae, Younbyoung,Um, Seo-In,Yi, Seung-Ho,Lee, Hyejung,Chang, Dong-Seon,Yin, Chang Shik,Park, Hi-Joon Elsevier 2011 Complementary Therapies in Medicine Vol.19 No.suppl1

<P><B>Summary</B></P><P><B>Objectives</B></P><P>A non-penetrating sham needle has been considered as a reliable control treatment in the field of acupuncture research. However, some concerns with regard to the credibility of sham needles have also been raised. We investigated whether there were differences in biomechanical properties and acupuncture sensation ratings in the process of needling between verum acupuncture (VA) and sham acupuncture (SA).</P><P><B>Methods</B></P><P>To quantify biomechanical force during needling in a cross-over design, we used a computer-controlled needling system. Fourteen participants received VA or SA at acupuncture point LI4, with no visual impact. They were asked to complete an acupuncture sensation form, including penetration, acute pain and <I>DeQi</I> sensations, and to identify the needling type they received.</P><P><B>Results</B></P><P>We found that there was a significant difference in insertion force between VA and SA (68.5±12.2 vs. 27.2±3.9gf; <I>p</I><0.001<B>)</B>. Participants clearly distinguished between the two kinds of stimulation and rated higher sensory ratings in the VA session (penetration sensation: 4.9±3.1 vs. 1.7±2.3, acute pain: 4.9±2.7 vs. 1.9±2.6, respectively; <I>p</I><0.01).</P><P><B>Conclusions</B></P><P>Our findings demonstrate that differential biomechanical forces such as insertion and pullout force contribute to the sensation of real and non-penetrating sham needles.</P>

Establishment of a humanized mouse model of keloid diseases following the migration of patient immune cells to the lesion: Patient-derived keloid xenograft (PDKX) model

Lee A Ram,Lee Seon-Yeong,Choi Jeong Won,Um In Gyu,Na Hyun Sik,Lee Jung Ho,Cho Mi-La 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Keloid disorder is an abnormal fibroproliferative reaction that can occur on any area of skin, and it can impair the quality of life of affected individuals. To investigate the pathogenesis and develop a treatment strategy, a preclinical animal model of keloid disorder is needed. However, keloid disorder is unique to humans, and the development of an animal model of keloid disorder is highly problematic. We developed the patient-derived keloid xenograft (PDKX), which is a humanized mouse model, and compared it to the traditional mouse xenograft model (transplantation of only keloid lesions). To establish the PDKX model, peripheral mononuclear cells (PBMCs) from ten keloid patients or five healthy control subjects were injected into NOD/SCID/IL-2Rγnull mice, and their keloid lesions were grafted onto the back after the engraftment of immune cells (transplantation of keloid lesions and KP PBMCs or HC PBMCs). Four weeks after surgery, the grafted keloid lesion was subjected to histologic evaluation. Compared to the traditional model, neotissue formed along the margin of the grafted skin, and lymphocyte infiltration and collagen synthesis were significantly elevated in the PDKX model. The neotissue sites resembled the margin areas of keloids in several respects. In detail, the levels of human Th17 cells, IL-17, HIF-1a, and chemokines were significantly elevated in the neotissue of the PDKX model. Furthermore, the weight of the keloid lesion was increased significantly in the PDKX model, which was due to the proinflammatory microenvironment of the keloid lesion. We confirmed that our patient-derived keloid xenograft (PDKX) model mimicked keloid disorder by recapitulating the in vivo microenvironment. This model will contribute to the investigation of cellular mechanisms and therapeutic treatments for keloid disorders.

신경초종 진단에 있어 자기공명영상촬영(MRI)의 진단적 가치

김정일,김엄지,문태용,이인숙,송유선,최경운,Kim, Jeung Il,Kim, Um Ji,Moon, Tae Yong,Lee, In Sook,Song, You Seon,Choi, Kyung Un 대한근골격종양학회 2014 대한골관절종양학회지 Vol.20 No.2

목적: 연부조직 종양 중 지방종과 신경초종(Schwannoma)은 자기공명영상촬영(MRI)만으로 충분한 진단적 가치를 가지는 종양이다. 하지만 신경초종의 경우 종양의 특성상 MRI에서 다양한 형태의 영상으로 나타날 수 있고, 신경주변에 생긴 다른 종양이 MRI상 신경초종으로 오인되는 경우도 드물지 않게 있다. 따라서 MRI상 신경초종으로 의심되는 환자를 대상으로 하여 MRI에서 신경초종을 감별할 수 있는 인자들을 찾아보고자 하였다. 대상 및 방법: 2002년 1월부터 2013년 5월까지 술 전 자기공명영상촬영(MRI)상 신경초종(schwannoma)이 의심된 환자 104예를 대상으로 하였으며 모든 환자에서 수술적 치료를 통한 조직검사를 시행하여 최종 진단 하였다. 조직 검사 결과 신경초종이 나온 경우를 1군으로 하고, 다른 병변이 나온 경우를 2군으로 나누어서 MRI 상 신경초종을 의심할 수 있는 영상의학적 소견을 비교 분석하였다. 결과: 104예의 조직검사 결과 신경초종으로 진단된 경우는 92예이며, 신경초종 외에 다른 병변으로 진단된 경우는 12예였다. MRI 특이 소견중 target sign은 1군에서는 41예(45%), 2군에서는 없었으며, fascicular sign은 1군에서는 47예(51%), 2군에서는 2예(17%), fat split sign은 1군에서는 44예(48%), 2군에서는 5예(42%), nerve entering and exiting sign은 1군에서는 28예(30%), 2군에서는 1예(9%)가 나타났다. 위 네가지 소견 모두 나타나지 않은 경우는 1군에서 8예(9%), 2군에서 6예(50%) 였다. 종양이 신경 주행 경로에 있는 경우는 1군에서 52예(57%), 2군에서 5예(42%)였다. Chi-squared test를 이용한 통계학적 분석에서, 위의 네가지 소견 중 target sign이 신경초종의 진단과 유의한 관계를 보였다(p<0.05). 결론: 신경초종 진단에 MRI가 중추적인 역할을 하고 있지만, 신경초종이 지닌 여러가지 특성으로 인해 MRI상 신경초종으로 의심된 경우라고 하더라도 최종적으로는 다른 종양으로 확진 된 경우가 드물지 않게 있다. 그러므로 MRI상 과녁징후가 보이지 않는 신경초종의 경우에는 다른 종양의 가능성도 염두에 두고 접근을 해야 할 것으로 보인다. Purpose: To diagnose soft tissue tumor, such as lipoma and Schwannoma, magnetic resonance imaging (MRI) is sufficient in most cases. However, various characteristics are found in MRI images of Schwannoma, thus other type of tumors are often misdiagnosed as Schwannoma with MRI images. In this study, we evaluate the diagnostic value of specific MRI findings of Schwannoma. Materials and Methods: From January 2002 to May 2013, 104 patients who are suspected as Schwannoma rith MRI images are included in data, and the final diagnosis is confirmed with biopsy. Patients are divided into group 1 and group 2 who are confirmed as Schwannoma and other disease with biopsy, respectively. Results: 92 patients were diagnosed as Schwannoma (group 1) and 12 patients were diagnosed as other disease (group 2). We investigate the diagnostic value of specific MRI findings of Schwannoma. 41 patients of group 1 (45%) and 0 patients of group 2 (0%) showed target sign, 47 patients of group 1 (51%) and 2 patients of group 2 (17%) showed fascicular sign, 44 patients of group 1 (48%) and 5 patients of group 2 (42%) showed fat split sign, 28 patients of group 1 (30%) and 1 patients of group 2(9%) showed nerve entering and exiting sign, and 8 patients of group 1 (9%) and 6 patients of group 2 (50%) showed none of four specific findings on their MRI images. 52 patients of group 1 (57%) and 5 patients of group 2 (42%) have tumors on the pathway of nerve. Target sign could be considered as the best diagnostic value of the sign we investigate (p<0.05). Conclusion: Although specific MRI findings have powerful diagnostic value, patients are often misdiagnosed as Schwannoma with MRI findings. Therefore, if patients who are suspected as Schwannoma based on MRI findings have no target sign on their MRI images, we should consider the possibility of other disease.